UniProtKB - P59923 (ZN445_HUMAN)
Zinc finger protein 445
ZNF445
Functioni
Transcription regulator required to maintain maternal and paternal gene imprinting, a process by which gene expression is restricted in a parent of origin-specific manner by epigenetic modification of genomic DNA and chromatin, including DNA methylation. Acts by controlling DNA methylation during the earliest multicellular stages of development at multiple imprinting control regions (ICRs) (PubMed:30602440).
Acts together with ZFP57, but seems to be the major factor in human early embryonic imprinting maintenance. In contrast, in mice, ZFP57 plays the predominant role in imprinting maintenance (PubMed:30602440).
1 PublicationRegions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 485 – 507 | C2H2-type 1PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 513 – 535 | C2H2-type 2PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 541 – 563 | C2H2-type 3PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 597 – 619 | C2H2-type 4PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 625 – 647 | C2H2-type 5PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 681 – 703 | C2H2-type 6PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 709 – 731 | C2H2-type 7PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 762 – 784 | C2H2-type 8PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 790 – 812 | C2H2-type 9PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 840 – 862 | C2H2-type 10PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 868 – 890 | C2H2-type 11PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 896 – 918 | C2H2-type 12PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 978 – 1000 | C2H2-type 13PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 1006 – 1028 | C2H2-type 14PROSITE-ProRule annotationAdd BLAST | 23 |
GO - Molecular functioni
- chromatin binding Source: Ensembl
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: GO_Central
- double-stranded methylated DNA binding Source: UniProtKB
- metal ion binding Source: UniProtKB-KW
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: GO_Central
GO - Biological processi
- maintenance of DNA methylation Source: UniProtKB
- regulation of genetic imprinting Source: UniProtKB
- regulation of transcription by RNA polymerase II Source: GO_Central
Keywordsi
Molecular function | DNA-binding |
Biological process | Transcription, Transcription regulation |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
PathwayCommonsi | P59923 |
Reactomei | R-HSA-212436, Generic Transcription Pathway |
SignaLinki | P59923 |
Names & Taxonomyi
Protein namesi | Recommended name: Zinc finger protein 445CuratedShort name: ZFP4451 Publication Alternative name(s): Zinc finger protein 168 Zinc finger protein with KRAB and SCAN domains 15 |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:21018, ZNF445 |
MIMi | 619508, gene |
neXtProti | NX_P59923 |
VEuPathDBi | HostDB:ENSG00000185219 |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Note: Binds various differentially methylated regions (DMR).1 Publication
Nucleus
- nucleus Source: UniProtKB
Keywords - Cellular componenti
NucleusPathology & Biotechi
Organism-specific databases
OpenTargetsi | ENSG00000185219 |
PharmGKBi | PA134904986 |
Miscellaneous databases
Pharosi | P59923, Tdark |
Genetic variation databases
BioMutai | ZNF445 |
DMDMi | 45593854 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000047594 | 1 – 1031 | Zinc finger protein 445Add BLAST | 1031 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 28 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)Combined sources | ||
Cross-linki | 317 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Cross-linki | 374 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Cross-linki | 375 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Cross-linki | 399 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Cross-linki | 567 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Cross-linki | 654 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Cross-linki | 736 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Cross-linki | 758 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Cross-linki | 938 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Cross-linki | 956 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Cross-linki | 975 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources |
Keywords - PTMi
Isopeptide bond, Ubl conjugationProteomic databases
EPDi | P59923 |
jPOSTi | P59923 |
MassIVEi | P59923 |
MaxQBi | P59923 |
PaxDbi | P59923 |
PeptideAtlasi | P59923 |
PRIDEi | P59923 |
ProteomicsDBi | 57172 |
PTM databases
iPTMneti | P59923 |
PhosphoSitePlusi | P59923 |
Expressioni
Developmental stagei
Gene expression databases
Bgeei | ENSG00000185219, Expressed in amniotic fluid and 225 other tissues |
ExpressionAtlasi | P59923, baseline and differential |
Genevisiblei | P59923, HS |
Organism-specific databases
HPAi | ENSG00000185219, Low tissue specificity |
Interactioni
Protein-protein interaction databases
BioGRIDi | 131672, 56 interactors |
IntActi | P59923, 21 interactors |
MINTi | P59923 |
STRINGi | 9606.ENSP00000413073 |
Miscellaneous databases
RNActi | P59923, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 55 – 137 | SCAN boxPROSITE-ProRule annotationAdd BLAST | 83 | |
Domaini | 234 – 304 | KRABPROSITE-ProRule annotationAdd BLAST | 71 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 43 | DisorderedSequence analysisAdd BLAST | 43 | |
Regioni | 911 – 939 | DisorderedSequence analysisAdd BLAST | 29 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 21 – 36 | Basic and acidic residuesSequence analysisAdd BLAST | 16 |
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 485 – 507 | C2H2-type 1PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 513 – 535 | C2H2-type 2PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 541 – 563 | C2H2-type 3PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 597 – 619 | C2H2-type 4PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 625 – 647 | C2H2-type 5PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 681 – 703 | C2H2-type 6PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 709 – 731 | C2H2-type 7PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 762 – 784 | C2H2-type 8PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 790 – 812 | C2H2-type 9PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 840 – 862 | C2H2-type 10PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 868 – 890 | C2H2-type 11PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 896 – 918 | C2H2-type 12PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 978 – 1000 | C2H2-type 13PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 1006 – 1028 | C2H2-type 14PROSITE-ProRule annotationAdd BLAST | 23 |
Keywords - Domaini
Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | KOG1721, Eukaryota |
GeneTreei | ENSGT00900000141186 |
HOGENOMi | CLU_002678_23_0_1 |
InParanoidi | P59923 |
OMAi | LHQETHT |
OrthoDBi | 1318335at2759 |
PhylomeDBi | P59923 |
TreeFami | TF350827 |
Family and domain databases
CDDi | cd07765, KRAB_A-box, 1 hit cd07936, SCAN, 1 hit |
Gene3Di | 1.10.4020.10, 1 hit |
InterProi | View protein in InterPro IPR001909, KRAB IPR036051, KRAB_dom_sf IPR003309, SCAN_dom IPR038269, SCAN_sf IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type |
Pfami | View protein in Pfam PF01352, KRAB, 1 hit PF02023, SCAN, 1 hit PF00096, zf-C2H2, 11 hits |
SMARTi | View protein in SMART SM00349, KRAB, 1 hit SM00431, SCAN, 1 hit SM00355, ZnF_C2H2, 14 hits |
SUPFAMi | SSF109640, SSF109640, 1 hit SSF57667, SSF57667, 9 hits |
PROSITEi | View protein in PROSITE PS50805, KRAB, 1 hit PS50804, SCAN_BOX, 1 hit PS00028, ZINC_FINGER_C2H2_1, 14 hits PS50157, ZINC_FINGER_C2H2_2, 14 hits |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MPPGRWHAAY PAQAQSSRER GRLQTVKKEE EDESYTPVQA ARPQTLNRPG
60 70 80 90 100
QELFRQLFRQ LRYHESSGPL ETLSRLRELC RWWLRPDVLS KAQILELLVL
110 120 130 140 150
EQFLSILPGE LRVWVQLHNP ESGEEAVALL EELQRDLDGT SWRDPGPAQS
160 170 180 190 200
PDVHWMGTGA LRSAQIWSLA SPLRSSSALG DHLEPPYEIE ARDFLAGQSD
210 220 230 240 250
TPAAQMPALF PREGCPGDQV TPTRSLTAQL QETMTFKDVE VTFSQDEWGW
260 270 280 290 300
LDSAQRNLYR DVMLENYRNM ASLVGPFTKP ALISWLEARE PWGLNMQAAQ
310 320 330 340 350
PKGNPVAAPT GDDLQSKTNK FILNQEPLEE AETLAVSSGC PATSVSEGIG
360 370 380 390 400
LRESFQQKSR QKDQCENPIQ VRVKKEETNF SHRTGKDSEV SGSNSLDLKH
410 420 430 440 450
VTYLRVSGRK ESLKHGCGKH FRMSSHHYDY KKYGKGLRHM IGGFSLHQRI
460 470 480 490 500
HSGLKGNKKD VCGKDFSLSS HHQRGQSLHT VGVSFKCSDC GRTFSHSSHL
510 520 530 540 550
AYHQRLHTQE KAFKCRVCGK AFRWSSNCAR HEKIHTGVKP YKCDLCEKAF
560 570 580 590 600
RRLSAYRLHR ETHAKKKFLE LNQYRAALTY SSGFDHHLGD QSGEKLFDCS
610 620 630 640 650
QCRKSFHCKS YVLEHQRIHT QEKPYKCTKC RKTFRWRSNF TRHMRLHEEE
660 670 680 690 700
KFYKQDECRE GFRQSPDCSQ PQGAPAVEKT FLCQQCGKTF TRKKTLVDHQ
710 720 730 740 750
RIHTGEKPYQ CSDCGKDFAY RSAFIVHKKK HAMKRKPEGG PSFSQDTVFQ
760 770 780 790 800
VPQSSHSKEE PYKCSQCGKA FRNHSFLLIH QRVHTGEKPY KCRECGKAFR
810 820 830 840 850
WSSNLYRHQR IHSLQKQYDC HESEKTPNVE PKILTGEKRF WCQECGKTFT
860 870 880 890 900
RKRTLLDHKG IHSGEKRYKC NLCGKSYDRN YRLVNHQRIH STERPFKCQW
910 920 930 940 950
CGKEFIGRHT LSSHQRKHTR AAQAERSPPA RSSSQDTKLR LQKLKPSEEM
960 970 980 990 1000
PLEDCKEACS QSSRLTGLQD ISIGKKCHKC SICGKTFNKS SQLISHKRFH
1010 1020 1030
TRERPFKCSK CGKTFRWSSN LARHMKNHIR D
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A087X0J9 | A0A087X0J9_HUMAN | Zinc finger protein 445 | ZNF445 | 273 | Annotation score: |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_052832 | 428 | Y → C. Corresponds to variant dbSNP:rs11710965Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY262260 mRNA Translation: AAP36990.1 AY295873 mRNA Translation: AAP50258.1 BC101486 mRNA Translation: AAI01487.1 |
CCDSi | CCDS2713.1 |
RefSeqi | NP_852466.1, NM_181489.5 XP_005265159.1, XM_005265102.2 XP_011531976.1, XM_011533674.2 |
Genome annotation databases
Ensembli | ENST00000396077; ENSP00000379387; ENSG00000185219 ENST00000425708; ENSP00000413073; ENSG00000185219 ENST00000634579; ENSP00000489139; ENSG00000283034 ENST00000635479; ENSP00000489368; ENSG00000283034 |
GeneIDi | 353274 |
KEGGi | hsa:353274 |
MANE-Selecti | ENST00000396077.8; ENSP00000379387.2; NM_181489.6; NP_852466.1 |
UCSCi | uc003cnf.3, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY262260 mRNA Translation: AAP36990.1 AY295873 mRNA Translation: AAP50258.1 BC101486 mRNA Translation: AAI01487.1 |
CCDSi | CCDS2713.1 |
RefSeqi | NP_852466.1, NM_181489.5 XP_005265159.1, XM_005265102.2 XP_011531976.1, XM_011533674.2 |
3D structure databases
SMRi | P59923 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 131672, 56 interactors |
IntActi | P59923, 21 interactors |
MINTi | P59923 |
STRINGi | 9606.ENSP00000413073 |
PTM databases
iPTMneti | P59923 |
PhosphoSitePlusi | P59923 |
Genetic variation databases
BioMutai | ZNF445 |
DMDMi | 45593854 |
Proteomic databases
EPDi | P59923 |
jPOSTi | P59923 |
MassIVEi | P59923 |
MaxQBi | P59923 |
PaxDbi | P59923 |
PeptideAtlasi | P59923 |
PRIDEi | P59923 |
ProteomicsDBi | 57172 |
Protocols and materials databases
Antibodypediai | 29430, 48 antibodies from 13 providers |
DNASUi | 353274 |
Genome annotation databases
Ensembli | ENST00000396077; ENSP00000379387; ENSG00000185219 ENST00000425708; ENSP00000413073; ENSG00000185219 ENST00000634579; ENSP00000489139; ENSG00000283034 ENST00000635479; ENSP00000489368; ENSG00000283034 |
GeneIDi | 353274 |
KEGGi | hsa:353274 |
MANE-Selecti | ENST00000396077.8; ENSP00000379387.2; NM_181489.6; NP_852466.1 |
UCSCi | uc003cnf.3, human |
Organism-specific databases
CTDi | 353274 |
GeneCardsi | ZNF445 |
HGNCi | HGNC:21018, ZNF445 |
HPAi | ENSG00000185219, Low tissue specificity |
MIMi | 619508, gene |
neXtProti | NX_P59923 |
OpenTargetsi | ENSG00000185219 |
PharmGKBi | PA134904986 |
VEuPathDBi | HostDB:ENSG00000185219 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1721, Eukaryota |
GeneTreei | ENSGT00900000141186 |
HOGENOMi | CLU_002678_23_0_1 |
InParanoidi | P59923 |
OMAi | LHQETHT |
OrthoDBi | 1318335at2759 |
PhylomeDBi | P59923 |
TreeFami | TF350827 |
Enzyme and pathway databases
PathwayCommonsi | P59923 |
Reactomei | R-HSA-212436, Generic Transcription Pathway |
SignaLinki | P59923 |
Miscellaneous databases
BioGRID-ORCSi | 353274, 18 hits in 1078 CRISPR screens |
ChiTaRSi | ZNF445, human |
GenomeRNAii | 353274 |
Pharosi | P59923, Tdark |
PROi | PR:P59923 |
RNActi | P59923, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000185219, Expressed in amniotic fluid and 225 other tissues |
ExpressionAtlasi | P59923, baseline and differential |
Genevisiblei | P59923, HS |
Family and domain databases
CDDi | cd07765, KRAB_A-box, 1 hit cd07936, SCAN, 1 hit |
Gene3Di | 1.10.4020.10, 1 hit |
InterProi | View protein in InterPro IPR001909, KRAB IPR036051, KRAB_dom_sf IPR003309, SCAN_dom IPR038269, SCAN_sf IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type |
Pfami | View protein in Pfam PF01352, KRAB, 1 hit PF02023, SCAN, 1 hit PF00096, zf-C2H2, 11 hits |
SMARTi | View protein in SMART SM00349, KRAB, 1 hit SM00431, SCAN, 1 hit SM00355, ZnF_C2H2, 14 hits |
SUPFAMi | SSF109640, SSF109640, 1 hit SSF57667, SSF57667, 9 hits |
PROSITEi | View protein in PROSITE PS50805, KRAB, 1 hit PS50804, SCAN_BOX, 1 hit PS00028, ZINC_FINGER_C2H2_1, 14 hits PS50157, ZINC_FINGER_C2H2_2, 14 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | ZN445_HUMAN | |
Accessioni | P59923Primary (citable) accession number: P59923 Secondary accession number(s): Q3MJD1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 10, 2003 |
Last sequence update: | October 10, 2003 | |
Last modified: | February 23, 2022 | |
This is version 154 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 3
Human chromosome 3: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families