UniProtKB - P59712 (NCAP_CVBQ)
Protein
Nucleoprotein
Gene
N
Organism
Bovine coronavirus (strain Quebec) (BCoV) (BCV)
Status
Functioni
Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication.UniRule annotation
GO - Molecular functioni
- RNA binding Source: UniProtKB-UniRule
Keywordsi
Molecular function | Ribonucleoprotein, RNA-binding, Viral nucleoprotein |
Biological process | Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: NucleoproteinUniRule annotationAlternative name(s): Nucleocapsid proteinUniRule annotation Short name: NCUniRule annotation Short name: Protein NUniRule annotation |
Gene namesi | Name:NUniRule annotation ORF Names:7a |
Organismi | Bovine coronavirus (strain Quebec) (BCoV) (BCV) |
Taxonomic identifieri | 11133 [NCBI] |
Taxonomic lineagei | Viruses › Riboviria › Orthornavirae › Pisuviricota › Pisoniviricetes › Nidovirales › Cornidovirineae › Coronaviridae › Orthocoronavirinae › Betacoronavirus › Embecovirus › |
Virus hosti | Bos taurus (Bovine) [TaxID: 9913] |
Proteomesi |
|
Subcellular locationi
- Virion UniRule annotation
- Host endoplasmic reticulum-Golgi intermediate compartment UniRule annotation
- Host Golgi apparatus UniRule annotation Note: Located inside the virion, complexed with the viral RNA. Probably associates with ER-derived membranes where it participates in viral RNA synthesis and virus budding.UniRule annotation
GO - Cellular componenti
- host cell endoplasmic reticulum-Golgi intermediate compartment Source: UniProtKB-SubCell
- host cell Golgi apparatus Source: UniProtKB-SubCell
- viral nucleocapsid Source: UniProtKB-UniRule
Keywords - Cellular componenti
Host Golgi apparatus, VirionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000105996 | 1 – 448 | NucleoproteinAdd BLAST | 448 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 167 | Phosphoserine; by hostUniRule annotation | 1 | |
Modified residuei | 174 | Phosphothreonine; by hostUniRule annotation | 1 | |
Modified residuei | 191 | Phosphoserine; by hostUniRule annotation | 1 | |
Modified residuei | 390 | Phosphoserine; by hostUniRule annotation | 1 | |
Modified residuei | 423 | Phosphoserine; by hostUniRule annotation | 1 | |
Modified residuei | 427 | Phosphothreonine; by hostUniRule annotation | 1 |
Post-translational modificationi
ADP-ribosylated. The ADP-ribosylation is retained in the virion during infection.UniRule annotation
Phosphorylated on serine and threonine residues.UniRule annotation
Keywords - PTMi
ADP-ribosylation, PhosphoproteinInteractioni
Subunit structurei
Homooligomer. Both monomeric and oligomeric forms interact with RNA.
Interacts with protein M.
Interacts with NSP3; this interaction serves to tether the genome to the newly translated replicase-transcriptase complex at a very early stage of infection.
UniRule annotationFamily & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 61 – 190 | CoV N NTDPROSITE-ProRule annotationAdd BLAST | 130 | |
Domaini | 259 – 384 | CoV N CTDPROSITE-ProRule annotationAdd BLAST | 126 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 52 – 194 | RNA-bindingUniRule annotationAdd BLAST | 143 | |
Regioni | 266 – 384 | DimerizationUniRule annotationAdd BLAST | 119 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 1 – 19 | Ser-richAdd BLAST | 19 | |
Compositional biasi | 191 – 219 | Ser-richAdd BLAST | 29 |
Sequence similaritiesi
Belongs to the betacoronavirus nucleocapsid protein family.UniRule annotation
Family and domain databases
HAMAPi | MF_04096, BETA_CORONA_NCAP, 1 hit |
InterProi | View protein in InterPro IPR043505, NCAP_bCoV IPR001218, Nucleocap_CoV IPR037179, Nucleocapsid_C IPR037195, Nucleocapsid_N |
Pfami | View protein in Pfam PF00937, CoV_nucleocap, 1 hit |
PIRSFi | PIRSF003888, Corona_nucleocap, 1 hit |
SUPFAMi | SSF103068, SSF103068, 1 hit SSF110304, SSF110304, 1 hit |
PROSITEi | View protein in PROSITE PS51929, COV_N_CTD, 1 hit PS51928, COV_N_NTD, 1 hit |
i Sequence
Sequence statusi: Complete.
P59712-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSFTPGKQSS SRASFGNRSG NGILKWADQS DQSRNVQTRG RRAQPKQTAT
60 70 80 90 100
SQLPSGGNVV PYYSWFSGIT QFQKGKEFEF AEGQGVPIAP GVPATEAKGY
110 120 130 140 150
WYRHNRRSFK TADGNQRQLL PRWYFYYLGT GPHAKDQYGT DIDGVFWVAS
160 170 180 190 200
NQADVNTPAD ILDRDPSSDE AIPTRFPPGT VLPQGYYIEG SGRSAPNSRS
210 220 230 240 250
TSRASSRASS AGSRSRANSG NRTPTSGVTP DMADQIASLV LAKLGKDATK
260 270 280 290 300
PQQVTKQTAK EIRQKILNKP RQKRSPNKQC TVQQCFGKRG PNQNFGGGEM
310 320 330 340 350
LKLGTSDPQF PILAELAPTA GAFFFGSRLE LAKVQNLSGN LDEPQKDVYE
360 370 380 390 400
LRYNGAIRFD STLSGFETIM KVLNENLNAY QQQDGMMNMS PKPQRQRGQK
410 420 430 440
NGQGENDNIS VAAPKSRVQQ NKSRELTAED ISLLKKMDEP YTEDTSEI
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF220295 Genomic RNA Translation: AAL40406.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF220295 Genomic RNA Translation: AAL40406.1 |
3D structure databases
SMRi | P59712 |
ModBasei | Search... |
Family and domain databases
HAMAPi | MF_04096, BETA_CORONA_NCAP, 1 hit |
InterProi | View protein in InterPro IPR043505, NCAP_bCoV IPR001218, Nucleocap_CoV IPR037179, Nucleocapsid_C IPR037195, Nucleocapsid_N |
Pfami | View protein in Pfam PF00937, CoV_nucleocap, 1 hit |
PIRSFi | PIRSF003888, Corona_nucleocap, 1 hit |
SUPFAMi | SSF103068, SSF103068, 1 hit SSF110304, SSF110304, 1 hit |
PROSITEi | View protein in PROSITE PS51929, COV_N_CTD, 1 hit PS51928, COV_N_NTD, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NCAP_CVBQ | |
Accessioni | P59712Primary (citable) accession number: P59712 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 16, 2003 |
Last sequence update: | June 16, 2003 | |
Last modified: | October 7, 2020 | |
This is version 69 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |