UniProtKB - P59632 (AP3A_SARS)
ORF3a protein
3a
Functioni
Forms homotetrameric potassium sensitive ion channels (viroporin) and may modulate virus release. Up-regulates expression of fibrinogen subunits FGA, FGB and FGG in host lung epithelial cells. Induces apoptosis in cell culture. Down-regulates the type 1 interferon receptor by inducing serine phosphorylation within the IFN alpha-receptor subunit 1 (IFNAR1) degradation motif and increasing IFNAR1 ubiquitination.
4 PublicationsSites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 133 | Involved in polymerization | 1 |
GO - Molecular functioni
- identical protein binding Source: IntAct
- ion channel activity Source: UniProtKB-KW
GO - Biological processi
- inorganic cation transmembrane transport Source: ComplexPortal
- pore formation by virus in membrane of host cell Source: UniProtKB-KW
- protein complex oligomerization Source: UniProtKB-KW
- suppression by virus of host interferon receptor activity Source: UniProtKB-KW
- suppression by virus of host type I interferon-mediated signaling pathway Source: UniProtKB-KW
Keywordsi
Enzyme and pathway databases
Reactomei | R-HSA-9678110, Attachment and Entry R-HSA-9679509, Virion Assembly and Release R-HSA-9683673, Maturation of protein 3a R-HSA-9683701, Translation of Structural Proteins R-HSA-9686347, Microbial modulation of RIPK1-mediated regulated necrosis |
SIGNORi | P59632 |
Protein family/group databases
TCDBi | 1.A.57.1.1, the human sars coronavirus viroporin (sars-vp) |
Names & Taxonomyi
Protein namesi | Recommended name: ORF3a proteinAlternative name(s): Accessory protein 3a Protein 3a Protein U274 Protein X1 |
Gene namesi | ORF Names:3a |
Organismi | Severe acute respiratory syndrome coronavirus (SARS-CoV) |
Taxonomic identifieri | 694009 [NCBI] |
Taxonomic lineagei | Viruses › Riboviria › Orthornavirae › Pisuviricota › Pisoniviricetes › Nidovirales › Cornidovirineae › Coronaviridae › Orthocoronavirinae › Betacoronavirus › Sarbecovirus |
Virus hosti | Homo sapiens (Human) [TaxID: 9606] Paguma larvata (Masked palm civet) [TaxID: 9675] |
Proteomesi |
|
Subcellular locationi
- Virion
- Host Golgi apparatus membrane ; Multi-pass membrane protein
- Host cell membrane ; Multi-pass membrane protein
- Secreted
- Host cytoplasm Note: The cell surface expressed protein can undergo endocytosis. The protein is secreted in association with membranous structures.
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 34 | Extracellular1 PublicationAdd BLAST | 34 | |
Transmembranei | 35 – 55 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 56 – 78 | CytoplasmicSequence analysisAdd BLAST | 23 | |
Transmembranei | 79 – 99 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 100 – 104 | ExtracellularSequence analysis | 5 | |
Transmembranei | 105 – 125 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 126 – 274 | Cytoplasmic1 PublicationAdd BLAST | 149 |
Keywords - Cellular componenti
Host cell membrane, Host cytoplasm, Host Golgi apparatus, Host membrane, Membrane, Secreted, VirionPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 27 | S → G: Complete loss of O-glycosylation; when associated with A-28; A-32 and A-34. 1 Publication | 1 | |
Mutagenesisi | 28 | T → A: Complete loss of O-glycosylation; when associated with A-27; A-32 and A-34. 1 Publication | 1 | |
Mutagenesisi | 32 | T → A: Complete loss of O-glycosylation; when associated with A-27; A-28 and A-34. 1 Publication | 1 | |
Mutagenesisi | 34 | T → A: Complete loss of O-glycosylation; when associated with A-27; A-28 and A-32. 1 Publication | 1 | |
Mutagenesisi | 81 | C → A: No effect on polymerization. 1 Publication | 1 | |
Mutagenesisi | 117 | C → A: No effect on polymerization. 1 Publication | 1 | |
Mutagenesisi | 121 | C → A: No effect on polymerization. 1 Publication | 1 | |
Mutagenesisi | 127 | C → A: No effect on polymerization. 1 Publication | 1 | |
Mutagenesisi | 130 | C → A: No effect on polymerization. 1 Publication | 1 | |
Mutagenesisi | 133 | C → A: Almost complete loss of polymerization ability. 1 Publication | 1 | |
Mutagenesisi | 148 | C → A: No effect on polymerization. 1 Publication | 1 | |
Mutagenesisi | 157 | C → A: No effect on polymerization. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000106131 | 1 – 274 | ORF3a proteinAdd BLAST | 274 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 27 | O-linked (GalNAc...) serine; by hostSequence analysis | 1 | |
Glycosylationi | 28 | O-linked (GalNAc...) threonine; by host1 Publication | 1 | |
Glycosylationi | 32 | O-linked (GalNAc...) threonine; by host1 Publication | 1 | |
Glycosylationi | 34 | O-linked (GalNAc...) threonine; by hostSequence analysis | 1 |
Post-translational modificationi
Keywords - PTMi
GlycoproteinProteomic databases
PRIDEi | P59632 |
PTM databases
GlyGeni | P59632, 4 sites |
iPTMneti | P59632 |
Interactioni
Subunit structurei
Homotetramer composed of two homodimers linked non covalently.
Interacts with M, S and E proteins.
Also interacts with the accessory protein 7a.
3 PublicationsBinary interactionsi
P59632
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 4383917, 67 interactors |
ComplexPortali | CPX-6095, SARS-CoV 3a complex |
DIPi | DIP-61251N |
IntActi | P59632, 56 interactors |
MINTi | P59632 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 33 – 141 | CoV 3a-like viroporin TMPROSITE-ProRule annotationAdd BLAST | 109 | |
Domaini | 145 – 237 | CoV 3a-like viroporin CDPROSITE-ProRule annotationAdd BLAST | 93 |
Domaini
Keywords - Domaini
Transmembrane, Transmembrane helixFamily and domain databases
CDDi | cd21648, SARS-CoV-like_ORF3a, 1 hit |
InterProi | View protein in InterPro IPR024407, Protein_3a_bCoV |
Pfami | View protein in Pfam PF11289, bCoV_viroporin, 1 hit |
PROSITEi | View protein in PROSITE PS51967, COV_VIROPORIN_3A_CD, 1 hit PS51966, COV_VIROPORIN_3A_TM, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MDLFMRFFTL GSITAQPVKI DNASPASTVH ATATIPLQAS LPFGWLVIGV
60 70 80 90 100
AFLAVFQSAT KIIALNKRWQ LALYKGFQFI CNLLLLFVTI YSHLLLVAAG
110 120 130 140 150
MEAQFLYLYA LIYFLQCINA CRIIMRCWLC WKCKSKNPLL YDANYFVCWH
160 170 180 190 200
THNYDYCIPY NSVTDTIVVT EGDGISTPKL KEDYQIGGYS EDRHSGVKDY
210 220 230 240 250
VVVHGYFTEV YYQLESTQIT TDTGIENATF FIFNKLVKDP PNVQIHTIDG
260 270
SSGVANPAMD PIYDEPTTTT SVPL
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 11 | G → R in strain: Isolate Tor2, Isolate BJ02 and Isolate BJ03. | 1 | |
Natural varianti | 20 | I → T in strain: Isolate Shanghai LY. | 1 | |
Natural varianti | 29 | V → A in strain: Isolate Shanghai QXC1. | 1 | |
Natural varianti | 101 | M → K in strain: Isolate HKU-39849. | 1 | |
Natural varianti | 129 | L → F in strain: Isolate TWK. | 1 | |
Natural varianti | 136 | K → Q in strain: Isolate BJ01. | 1 | |
Natural varianti | 171 | E → A in strain: Isolate GD01. | 1 | |
Natural varianti | 193 | R → W in strain: Isolate GD01. | 1 | |
Natural varianti | 222 | D → N in strain: Isolate Shanghai QXC1. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY278741 Genomic RNA Translation: AAP13446.1 AY274119 Genomic RNA Translation: AAP41038.1 AY282752 Genomic RNA No translation available. AY278554 Genomic RNA Translation: AAP13568.1 AY278491 Genomic RNA No translation available. AY278487 Genomic RNA No translation available. AY278488 Genomic RNA Translation: AAP30031.1 AY278489 Genomic RNA Translation: AAP51228.1 AY278490 Genomic RNA No translation available. AY279354 Genomic RNA No translation available. AY291451 Genomic RNA Translation: AAP37018.1 AY310120 Genomic RNA Translation: AAP50486.1 AY291315 Genomic RNA Translation: AAP33698.1 AY338174 Genomic RNA Translation: AAQ01598.1 AY338175 Genomic RNA Translation: AAQ01610.1 AY348314 Genomic RNA Translation: AAP97883.1 AP006557 Genomic RNA Translation: BAC81349.1 AP006558 Genomic RNA Translation: BAC81363.1 AP006559 Genomic RNA Translation: BAC81377.1 AP006560 Genomic RNA Translation: BAC81391.1 AP006561 Genomic RNA Translation: BAC81405.1 AY323977 Genomic RNA Translation: AAP72975.1 AY427439 Genomic RNA Translation: AAQ94061.1 AY463059 Genomic RNA Translation: AAP82984.2 AY322207 Genomic RNA Translation: AAP82969.1 |
RefSeqi | NP_828852.2, NC_004718.3 |
Genome annotation databases
GeneIDi | 1489669 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY278741 Genomic RNA Translation: AAP13446.1 AY274119 Genomic RNA Translation: AAP41038.1 AY282752 Genomic RNA No translation available. AY278554 Genomic RNA Translation: AAP13568.1 AY278491 Genomic RNA No translation available. AY278487 Genomic RNA No translation available. AY278488 Genomic RNA Translation: AAP30031.1 AY278489 Genomic RNA Translation: AAP51228.1 AY278490 Genomic RNA No translation available. AY279354 Genomic RNA No translation available. AY291451 Genomic RNA Translation: AAP37018.1 AY310120 Genomic RNA Translation: AAP50486.1 AY291315 Genomic RNA Translation: AAP33698.1 AY338174 Genomic RNA Translation: AAQ01598.1 AY338175 Genomic RNA Translation: AAQ01610.1 AY348314 Genomic RNA Translation: AAP97883.1 AP006557 Genomic RNA Translation: BAC81349.1 AP006558 Genomic RNA Translation: BAC81363.1 AP006559 Genomic RNA Translation: BAC81377.1 AP006560 Genomic RNA Translation: BAC81391.1 AP006561 Genomic RNA Translation: BAC81405.1 AY323977 Genomic RNA Translation: AAP72975.1 AY427439 Genomic RNA Translation: AAQ94061.1 AY463059 Genomic RNA Translation: AAP82984.2 AY322207 Genomic RNA Translation: AAP82969.1 |
RefSeqi | NP_828852.2, NC_004718.3 |
3D structure databases
SMRi | P59632 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4383917, 67 interactors |
ComplexPortali | CPX-6095, SARS-CoV 3a complex |
DIPi | DIP-61251N |
IntActi | P59632, 56 interactors |
MINTi | P59632 |
Protein family/group databases
TCDBi | 1.A.57.1.1, the human sars coronavirus viroporin (sars-vp) |
PTM databases
GlyGeni | P59632, 4 sites |
iPTMneti | P59632 |
Proteomic databases
PRIDEi | P59632 |
Genome annotation databases
GeneIDi | 1489669 |
Enzyme and pathway databases
Reactomei | R-HSA-9678110, Attachment and Entry R-HSA-9679509, Virion Assembly and Release R-HSA-9683673, Maturation of protein 3a R-HSA-9683701, Translation of Structural Proteins R-HSA-9686347, Microbial modulation of RIPK1-mediated regulated necrosis |
SIGNORi | P59632 |
Family and domain databases
CDDi | cd21648, SARS-CoV-like_ORF3a, 1 hit |
InterProi | View protein in InterPro IPR024407, Protein_3a_bCoV |
Pfami | View protein in Pfam PF11289, bCoV_viroporin, 1 hit |
PROSITEi | View protein in PROSITE PS51967, COV_VIROPORIN_3A_CD, 1 hit PS51966, COV_VIROPORIN_3A_TM, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | AP3A_SARS | |
Accessioni | P59632Primary (citable) accession number: P59632 Secondary accession number(s): Q7T6R6 Q80BV5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 23, 2003 |
Last sequence update: | April 23, 2003 | |
Last modified: | February 23, 2022 | |
This is version 104 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |