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Protein

Eukaryotic translation initiation factor 5

Gene

Eif5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex (40S.mRNA.Met-tRNA[F].eIF-2.GTP) with the subsequent joining of a 60S ribosomal subunit resulting in the release of eIF-2 and the guanine nucleotide. The subsequent joining of a 60S ribosomal subunit results in the formation of a functional 80S initiation complex (80S.mRNA.Met-tRNA[F]).

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi27 – 34GTPSequence analysis8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionInitiation factor
Biological processProtein biosynthesis
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-72702 Ribosomal scanning and start codon recognition

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 5
Short name:
eIF-5
Gene namesi
Name:Eif5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:95309 Eif5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002125171 – 429Eukaryotic translation initiation factor 5Add BLAST429

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei10PhosphoserineBy similarity1
Modified residuei225PhosphothreonineBy similarity1
Modified residuei227PhosphoserineCombined sources1
Modified residuei387PhosphoserineCombined sources1
Modified residuei388PhosphoserineCombined sources1
Modified residuei408PhosphoserineBy similarity1
Cross-linki411Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki416Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei417PhosphoserineCombined sources1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP59325
PaxDbiP59325
PeptideAtlasiP59325
PRIDEiP59325

PTM databases

CarbonylDBiP59325
iPTMnetiP59325
PhosphoSitePlusiP59325
SwissPalmiP59325

Expressioni

Gene expression databases

BgeeiENSMUSG00000021282 Expressed in 307 organ(s), highest expression level in ear vesicle
CleanExiMM_EIF5
ExpressionAtlasiP59325 baseline and differential
GenevisibleiP59325 MM

Interactioni

Subunit structurei

Interacts with FMR1; this interaction occurs in a RNA-dependent manner.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi229973, 1 interactor
IntActiP59325, 3 interactors
MINTiP59325
STRINGi10090.ENSMUSP00000061616

Structurei

3D structure databases

ProteinModelPortaliP59325
SMRiP59325
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini231 – 390W2PROSITE-ProRule annotationAdd BLAST160

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi194 – 200Asp/Glu-rich (highly acidic)7
Compositional biasi382 – 400Asp/Glu-rich (highly acidic)Add BLAST19
Compositional biasi421 – 427Asp-rich (acidic)7

Sequence similaritiesi

Belongs to the eIF-2-beta/eIF-5 family.Curated

Phylogenomic databases

eggNOGiKOG2767 Eukaryota
COG1601 LUCA
GeneTreeiENSGT00390000016478
HOGENOMiHOG000214327
HOVERGENiHBG006132
InParanoidiP59325
KOiK03262
OMAiDDKFYRY
OrthoDBiEOG091G0AKW
PhylomeDBiP59325
TreeFamiTF101533

Family and domain databases

Gene3Di1.25.40.180, 1 hit
InterProiView protein in InterPro
IPR016024 ARM-type_fold
IPR016021 MIF4-like_sf
IPR002735 Transl_init_fac_IF2/IF5
IPR016189 Transl_init_fac_IF2/IF5_N
IPR016190 Transl_init_fac_IF2/IF5_Zn-bd
IPR003307 W2_domain
PfamiView protein in Pfam
PF01873 eIF-5_eIF-2B, 1 hit
PF02020 W2, 1 hit
SMARTiView protein in SMART
SM00653 eIF2B_5, 1 hit
SM00515 eIF5C, 1 hit
SUPFAMiSSF100966 SSF100966, 1 hit
SSF48371 SSF48371, 1 hit
SSF75689 SSF75689, 1 hit
PROSITEiView protein in PROSITE
PS51363 W2, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 7 potential isoforms that are computationally mapped.Show allAlign All

P59325-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSVNVNRSVS DQFYRYKMPR LIAKVEGKGN GIKTVIVNMV DVAKALNRPP
60 70 80 90 100
TYPTKYFGCE LGAQTQFDVK NDRYIVNGSH EANKLQDMLD GFIKKFVLCP
110 120 130 140 150
ECENPETDLH VNPKKQTIGN SCKACGYRGM LDTHHKLCTF ILKNPPENSD
160 170 180 190 200
IGTGKKEKEK KNRKGKDKEN GSVSTSETPP PPPPNEISPP HAVEEEEDDD
210 220 230 240 250
WGEDTTEEAQ RRRMDEISDH AKGLTLSDDL ERTVEERVNI LFDFVKKKKE
260 270 280 290 300
EGIIDSSDKE IVAEAERLDV KAMGPLVLTE VLFDEKIREQ IKKYRRHFLR
310 320 330 340 350
FCHNNKKAQR YLLHGLECVV AMHQAQLISK IPHILKEMYD ADLLEEEVII
360 370 380 390 400
SWSEKASKKY VSKELAKEIR VKAEPFIKWL KEAEEESSGG EEEDEDENIE
410 420
VVYSKTASVP KVETVKSDNK DDDIDIDAI
Length:429
Mass (Da):48,968
Last modified:February 12, 2003 - v1
Checksum:iC77DD5DFBB5C1DEF
GO

Computationally mapped potential isoform sequencesi

There are 7 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q8BVV6Q8BVV6_MOUSE
Eukaryotic translation initiation f...
Eif5
375Annotation score:
A0A1Y7VNP4A0A1Y7VNP4_MOUSE
Eukaryotic translation initiation f...
Eif5
146Annotation score:
A0A1Y7VJG2A0A1Y7VJG2_MOUSE
Eukaryotic translation initiation f...
Eif5
159Annotation score:
A0A1Y7VLQ0A0A1Y7VLQ0_MOUSE
Eukaryotic translation initiation f...
Eif5
53Annotation score:
A0A1Y7VLL6A0A1Y7VLL6_MOUSE
Eukaryotic translation initiation f...
Eif5
34Annotation score:
A0A1Y7VLK2A0A1Y7VLK2_MOUSE
Eukaryotic translation initiation f...
Eif5
90Annotation score:
A0A1Y7VKT5A0A1Y7VKT5_MOUSE
Eukaryotic translation initiation f...
Eif5
36Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC039275 mRNA Translation: AAH39275.1
BC042622 mRNA No translation available.
CCDSiCCDS26180.1
RefSeqiNP_775539.1, NM_173363.5
NP_829887.1, NM_178041.2
UniGeneiMm.271222
Mm.470192

Genome annotation databases

EnsembliENSMUST00000050993; ENSMUSP00000061616; ENSMUSG00000021282
ENSMUST00000166123; ENSMUSP00000126825; ENSMUSG00000021282
ENSMUST00000222375; ENSMUSP00000152791; ENSMUSG00000021282
GeneIDi217869
KEGGimmu:217869
UCSCiuc007pdd.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC039275 mRNA Translation: AAH39275.1
BC042622 mRNA No translation available.
CCDSiCCDS26180.1
RefSeqiNP_775539.1, NM_173363.5
NP_829887.1, NM_178041.2
UniGeneiMm.271222
Mm.470192

3D structure databases

ProteinModelPortaliP59325
SMRiP59325
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi229973, 1 interactor
IntActiP59325, 3 interactors
MINTiP59325
STRINGi10090.ENSMUSP00000061616

PTM databases

CarbonylDBiP59325
iPTMnetiP59325
PhosphoSitePlusiP59325
SwissPalmiP59325

Proteomic databases

EPDiP59325
PaxDbiP59325
PeptideAtlasiP59325
PRIDEiP59325

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000050993; ENSMUSP00000061616; ENSMUSG00000021282
ENSMUST00000166123; ENSMUSP00000126825; ENSMUSG00000021282
ENSMUST00000222375; ENSMUSP00000152791; ENSMUSG00000021282
GeneIDi217869
KEGGimmu:217869
UCSCiuc007pdd.2 mouse

Organism-specific databases

CTDi1983
MGIiMGI:95309 Eif5

Phylogenomic databases

eggNOGiKOG2767 Eukaryota
COG1601 LUCA
GeneTreeiENSGT00390000016478
HOGENOMiHOG000214327
HOVERGENiHBG006132
InParanoidiP59325
KOiK03262
OMAiDDKFYRY
OrthoDBiEOG091G0AKW
PhylomeDBiP59325
TreeFamiTF101533

Enzyme and pathway databases

ReactomeiR-MMU-72702 Ribosomal scanning and start codon recognition

Miscellaneous databases

ChiTaRSiEif5 mouse
PROiPR:P59325
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000021282 Expressed in 307 organ(s), highest expression level in ear vesicle
CleanExiMM_EIF5
ExpressionAtlasiP59325 baseline and differential
GenevisibleiP59325 MM

Family and domain databases

Gene3Di1.25.40.180, 1 hit
InterProiView protein in InterPro
IPR016024 ARM-type_fold
IPR016021 MIF4-like_sf
IPR002735 Transl_init_fac_IF2/IF5
IPR016189 Transl_init_fac_IF2/IF5_N
IPR016190 Transl_init_fac_IF2/IF5_Zn-bd
IPR003307 W2_domain
PfamiView protein in Pfam
PF01873 eIF-5_eIF-2B, 1 hit
PF02020 W2, 1 hit
SMARTiView protein in SMART
SM00653 eIF2B_5, 1 hit
SM00515 eIF5C, 1 hit
SUPFAMiSSF100966 SSF100966, 1 hit
SSF48371 SSF48371, 1 hit
SSF75689 SSF75689, 1 hit
PROSITEiView protein in PROSITE
PS51363 W2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiIF5_MOUSE
AccessioniPrimary (citable) accession number: P59325
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 12, 2003
Last sequence update: February 12, 2003
Last modified: September 12, 2018
This is version 131 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Translation initiation factors
    List of translation initiation factor entries
  3. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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