UniProtKB - P59216 (GNAO_CRILO)
Guanine nucleotide-binding protein G(o) subunit alpha
GNAO1
Functioni
Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(o) protein function is not clear. Stimulated by RGS14 (By similarity).
By similaritySites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 47 | MagnesiumBy similarity | 1 | |
Metal bindingi | 182 | MagnesiumBy similarity | 1 | |
Binding sitei | 326 | GTP; via amide nitrogenBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 40 – 47 | GTPBy similarity | 8 | |
Nucleotide bindingi | 176 – 182 | GTPBy similarity | 7 | |
Nucleotide bindingi | 201 – 205 | GTPBy similarity | 5 | |
Nucleotide bindingi | 270 – 273 | GTPBy similarity | 4 |
GO - Molecular functioni
- G-protein beta/gamma-subunit complex binding Source: InterPro
- GTPase activity Source: InterPro
- GTP binding Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
Keywordsi
Molecular function | Transducer |
Ligand | GTP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Guanine nucleotide-binding protein G(o) subunit alpha |
Gene namesi | Name:GNAO1 Synonyms:GNA0, GNAO |
Organismi | Cricetulus longicaudatus (Long-tailed dwarf hamster) (Chinese hamster) |
Taxonomic identifieri | 10030 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Cricetidae › Cricetinae › Cricetulus |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity
Other locations
- Membrane By similarity; Lipid-anchor By similarity
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000203700 | 2 – 354 | Guanine nucleotide-binding protein G(o) subunit alphaAdd BLAST | 353 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 2 | N-myristoyl glycineBy similarity | 1 | |
Lipidationi | 3 | S-palmitoyl cysteineBy similarity | 1 | |
Modified residuei | 205 | 5-glutamyl histamineBy similarity | 1 | |
Modified residuei | 346 | Deamidated asparagine; in form Alpha-3By similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Lipoprotein, Myristate, PalmitateProteomic databases
PRIDEi | P59216 |
Interactioni
Subunit structurei
Interacts with RGS14 (By similarity). G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.
By similarityFamily & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 32 – 354 | G-alphaPROSITE-ProRule annotationAdd BLAST | 323 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 35 – 48 | G1 motifPROSITE-ProRule annotationAdd BLAST | 14 | |
Regioni | 174 – 182 | G2 motifPROSITE-ProRule annotation | 9 | |
Regioni | 197 – 206 | G3 motifPROSITE-ProRule annotation | 10 | |
Regioni | 266 – 273 | G4 motifPROSITE-ProRule annotation | 8 | |
Regioni | 324 – 329 | G5 motifPROSITE-ProRule annotation | 6 |
Sequence similaritiesi
Family and domain databases
CDDi | cd00066, G-alpha, 1 hit |
Gene3Di | 1.10.400.10, 1 hit 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR001408, Gprotein_alpha_I IPR001019, Gprotein_alpha_su IPR011025, GproteinA_insert IPR027417, P-loop_NTPase |
PANTHERi | PTHR10218, PTHR10218, 1 hit |
Pfami | View protein in Pfam PF00503, G-alpha, 1 hit |
PRINTSi | PR00318, GPROTEINA PR00441, GPROTEINAI |
SMARTi | View protein in SMART SM00275, G_alpha, 1 hit |
SUPFAMi | SSF47895, SSF47895, 1 hit SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS51882, G_ALPHA, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MGCTLSAEER AALERSKAIE KNLKEDGISA AKDVKLLLLG AGESGKSTIV
60 70 80 90 100
KQMKIIHEDG FSGEDVKQYK PVVYSNTIQS LAAIVRAMDT LGVEYGDKER
110 120 130 140 150
KADSKMVCDV VSRMEDTEPF SAELLSAMMR LWGDSGIQEC FNRSREYQLN
160 170 180 190 200
DSAKYYLDSL DRIGAADYQP TEQDILRTRV KTTGIVETHF TFKNLHFRLF
210 220 230 240 250
DVGGQRSERK KWIHCFEDVT AIIFCVALSG YDQVLHEDET TNRMHESLML
260 270 280 290 300
FDSICNNKFF IDTSIILFLN KKDLFGEKIK KSPLTICFPE YPGSNTYEDA
310 320 330 340 350
AAYIQTQFES KNRSPNKEIY CHMTCATDTN NIQVVFDAVT DIIIANNLRG
CGLY
The sequence of this isoform differs from the canonical sequence as follows:
249-354: MLFDSICNNK...ANNLRGCGLY → KLFDSICNNK...AKNLRGCGLY
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_031249 | 249 – 354 | MLFDS…GCGLY → KLFDSICNNKWFTDTSIILF LNKKDIFEEKITRSPLTICF PEYTGPSAFTEAVAHIQGQY ESKNKSAHKEIYTHFTCATD TNNIQFVFDAVTDVIIAKNL RGCGLY in isoform Alpha-2. 1 PublicationAdd BLAST | 106 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M33661 mRNA Translation: AAA36987.1 M33662 mRNA Translation: AAA36988.1 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M33661 mRNA Translation: AAA36987.1 M33662 mRNA Translation: AAA36988.1 |
3D structure databases
SMRi | P59216 |
ModBasei | Search... |
Proteomic databases
PRIDEi | P59216 |
Family and domain databases
CDDi | cd00066, G-alpha, 1 hit |
Gene3Di | 1.10.400.10, 1 hit 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR001408, Gprotein_alpha_I IPR001019, Gprotein_alpha_su IPR011025, GproteinA_insert IPR027417, P-loop_NTPase |
PANTHERi | PTHR10218, PTHR10218, 1 hit |
Pfami | View protein in Pfam PF00503, G-alpha, 1 hit |
PRINTSi | PR00318, GPROTEINA PR00441, GPROTEINAI |
SMARTi | View protein in SMART SM00275, G_alpha, 1 hit |
SUPFAMi | SSF47895, SSF47895, 1 hit SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS51882, G_ALPHA, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | GNAO_CRILO | |
Accessioni | P59216Primary (citable) accession number: P59216 Secondary accession number(s): P04900, P17806 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 13, 1987 |
Last sequence update: | January 23, 2007 | |
Last modified: | September 29, 2021 | |
This is version 90 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |