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UniProtKB - P59078 (SYM_BRUSU)
Protein
Methionine--tRNA ligase
Gene
metG
Organism
Brucella suis biovar 1 (strain 1330)
Status
Functioni
Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation.
UniRule annotationCatalytic activityi
- EC:6.1.1.10UniRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 303 | ATPUniRule annotation | 1 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-UniRule
- methionine-tRNA ligase activity Source: UniProtKB-UniRule
GO - Biological processi
- methionyl-tRNA aminoacylation Source: UniProtKB-UniRule
Keywordsi
Molecular function | Aminoacyl-tRNA synthetase, Ligase |
Biological process | Protein biosynthesis |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Methionine--tRNA ligaseUniRule annotation (EC:6.1.1.10UniRule annotation)Alternative name(s): Methionyl-tRNA synthetaseUniRule annotation Short name: MetRSUniRule annotation |
Gene namesi | Name:metGUniRule annotation Ordered Locus Names:BR0995, BS1330_I0991 |
Organismi | Brucella suis biovar 1 (strain 1330) |
Taxonomic identifieri | 204722 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Alphaproteobacteria › Hyphomicrobiales › Brucellaceae › Brucella/Ochrobactrum group › Brucella › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotation
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000139213 | 1 – 515 | Methionine--tRNA ligaseAdd BLAST | 515 |
Interactioni
Subunit structurei
Monomer.
UniRule annotationStructurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P59078 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 13 – 23 | 'HIGH' regionAdd BLAST | 11 | |
Motifi | 300 – 304 | 'KMSKS' region | 5 |
Sequence similaritiesi
Phylogenomic databases
HOGENOMi | CLU_009710_9_4_5 |
OMAi | TYVWFDA |
PhylomeDBi | P59078 |
Family and domain databases
CDDi | cd07957, Anticodon_Ia_Met, 1 hit cd00814, MetRS_core, 1 hit |
Gene3Di | 3.40.50.620, 1 hit |
HAMAPi | MF_01228, Met_tRNA_synth_type2, 1 hit |
InterProi | View protein in InterPro IPR001412, aa-tRNA-synth_I_CS IPR041872, Anticodon_Met IPR014758, Met-tRNA_synth IPR023457, Met-tRNA_synth_2 IPR015413, Methionyl/Leucyl_tRNA_Synth IPR033911, MetRS_core IPR014729, Rossmann-like_a/b/a_fold IPR009080, tRNAsynth_Ia_anticodon-bd |
PANTHERi | PTHR43326, PTHR43326, 1 hit |
Pfami | View protein in Pfam PF19303, Anticodon_3, 1 hit PF09334, tRNA-synt_1g, 1 hit |
PRINTSi | PR01041, TRNASYNTHMET |
SUPFAMi | SSF47323, SSF47323, 1 hit |
TIGRFAMsi | TIGR00398, metG, 1 hit |
PROSITEi | View protein in PROSITE PS00178, AA_TRNA_LIGASE_I, 1 hit |
i Sequence
Sequence statusi: Complete.
P59078-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSREKYYITT AIAYPNGKPH IGHAYELIAT DAMARFQRLN GMDVYFLTGT
60 70 80 90 100
DEHGIKMLQS ARKEGITPRE LADRNTSAFR RMAEVLNSSN DDYIRTSEER
110 120 130 140 150
HYKASQAIWQ AMVANGDIYK GGYAGWYSVR DEAYYGEEET EVRADGVRYG
160 170 180 190 200
PQGTPVEWVE EESYFFRLSA YQDKLLDLYE NNPGFIMPAE RRNEIVSFVK
210 220 230 240 250
SGLKDLSISR TTFDWGIPVP GDEKHVMYVW VDALTNYITA LGYPDTTDER
260 270 280 290 300
WAYWPANAHI IGKDISRFHA VYWPAFLMSA QLPLPKRVFA HGFLFNRGEK
310 320 330 340 350
MSKSVGNVID PFELVERYGL DQLRYFLMRE VPFGQDGSYS HEAIVNRTNA
360 370 380 390 400
DLANDLGNLA QRSLSMIAKN CEGKVPQPGA FSEADKAILD QADAALETAR
410 420 430 440 450
KAMDDQALHL ALGAIFAVVA EANRYFAGQE PWALRKTDPA RMGTVLYVTA
460 470 480 490 500
EVLRRVGIMV QPFIPQSAEK LLDILAVPAD KRQFADVLAS PLAGGTDLPA
510
PQPVFPRYVE ADEQN
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE014291 Genomic DNA Translation: AAN29918.1 CP002997 Genomic DNA Translation: AEM18335.1 |
RefSeqi | WP_004689678.1, NZ_KN046804.1 |
Genome annotation databases
EnsemblBacteriai | AEM18335; AEM18335; BS1330_I0991 |
GeneIDi | 45052047 |
KEGGi | bms:BR0995 bsi:BS1330_I0991 |
PATRICi | fig|204722.21.peg.732 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE014291 Genomic DNA Translation: AAN29918.1 CP002997 Genomic DNA Translation: AEM18335.1 |
RefSeqi | WP_004689678.1, NZ_KN046804.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5K0S | X-ray | 2.45 | A/B/C | 1-515 | [»] | |
5K0T | X-ray | 2.60 | A/B/C | 1-515 | [»] | |
SMRi | P59078 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Genome annotation databases
EnsemblBacteriai | AEM18335; AEM18335; BS1330_I0991 |
GeneIDi | 45052047 |
KEGGi | bms:BR0995 bsi:BS1330_I0991 |
PATRICi | fig|204722.21.peg.732 |
Phylogenomic databases
HOGENOMi | CLU_009710_9_4_5 |
OMAi | TYVWFDA |
PhylomeDBi | P59078 |
Family and domain databases
CDDi | cd07957, Anticodon_Ia_Met, 1 hit cd00814, MetRS_core, 1 hit |
Gene3Di | 3.40.50.620, 1 hit |
HAMAPi | MF_01228, Met_tRNA_synth_type2, 1 hit |
InterProi | View protein in InterPro IPR001412, aa-tRNA-synth_I_CS IPR041872, Anticodon_Met IPR014758, Met-tRNA_synth IPR023457, Met-tRNA_synth_2 IPR015413, Methionyl/Leucyl_tRNA_Synth IPR033911, MetRS_core IPR014729, Rossmann-like_a/b/a_fold IPR009080, tRNAsynth_Ia_anticodon-bd |
PANTHERi | PTHR43326, PTHR43326, 1 hit |
Pfami | View protein in Pfam PF19303, Anticodon_3, 1 hit PF09334, tRNA-synt_1g, 1 hit |
PRINTSi | PR01041, TRNASYNTHMET |
SUPFAMi | SSF47323, SSF47323, 1 hit |
TIGRFAMsi | TIGR00398, metG, 1 hit |
PROSITEi | View protein in PROSITE PS00178, AA_TRNA_LIGASE_I, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | SYM_BRUSU | |
Accessioni | P59078Primary (citable) accession number: P59078 Secondary accession number(s): G0K9R9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 8, 2002 |
Last sequence update: | November 8, 2002 | |
Last modified: | February 23, 2022 | |
This is version 109 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structureDocuments
- Aminoacyl-tRNA synthetases
List of aminoacyl-tRNA synthetase entries - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families