UniProtKB - P58913 (O16A_CONPU)
Protein
Delta-conotoxin PVIA
Gene
N/A
Organism
Conus purpurascens (Purple cone)
Status
Functioni
Delta-conotoxins bind to site 6 of voltage-gated sodium channels (Nav) and inhibit the inactivation process. This toxin shows weak effects on rNav1.2/SCN2A (EC(50)=2.9 µM), rNav1.4/SCN4A (EC(50)=5.2 µM), hNav1.7/SCN9A (EC(50)=1.9 µM) and rNav1.7/SCN9A (EC(50)=6.4 µM) (PubMed:10627583). In vivo, this toxin shows different effects. In mice, injection of this toxin causes hyperactivity, rapid running, limb extension, and death. In fish, the peptide elicites spurts of rapid swimming, with twisted motions, quivering fins and the lockjaw extended mouth syndrome. Rigid paralysis and death are observed at higher doses. In mollusks, this peptide is inactive. Injection of this peptide together with the kappa-conotoxin PVIIA causes the sudden tetanus of prey (STOP) syndrome, which is a single, lethal "fin-pop" in envenomed fish.2 Publications
GO - Molecular functioni
- sodium channel inhibitor activity Source: InterPro
- toxin activity Source: UniProtKB-KW
Keywordsi
Molecular function | Ion channel impairing toxin, Neurotoxin, Toxin, Voltage-gated sodium channel impairing toxin |
Names & Taxonomyi
Protein namesi | Recommended name: Delta-conotoxin PVIA1 PublicationAlternative name(s): Lockjaw peptide1 Publication |
Organismi | Conus purpurascens (Purple cone) |
Taxonomic identifieri | 41690 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Lophotrochozoa › Mollusca › Gastropoda › Caenogastropoda › Neogastropoda › Conoidea › Conidae › Conus › Chelyconus |
Organism-specific databases
ConoServeri | 1563 PVIA precursor |
Subcellular locationi
Extracellular region or secreted
- Secreted 1 Publication
Extracellular region or secreted
- extracellular region Source: UniProtKB-SubCell
Keywords - Cellular componenti
SecretedPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 59 | T → A: No loss of activity. 1 Publication | 1 | |
Mutagenesisi | 60 | F → A: Loss of biological activity. However, coinjection of this mutant protects against the wild-type conotoxin, suggesting that the mutant binds competitively to the wild-type conotoxin ligand site. Such a protective effect does not occur for all physiological targets. 1 Publication | 1 | |
Mutagenesisi | 63 | I → A: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 64 | K → A: No loss of activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 22 | Sequence analysisAdd BLAST | 22 | |
PropeptideiPRO_0000034898 | 23 – 49 | 1 PublicationAdd BLAST | 27 | |
PeptideiPRO_0000034899 | 52 – 80 | Delta-conotoxin PVIA1 PublicationAdd BLAST | 29 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 54 ↔ 69 | By similarity | ||
Modified residuei | 57 | 4-hydroxyproline1 Publication | 1 | |
Disulfide bondi | 61 ↔ 73 | By similarity | ||
Modified residuei | 65 | 4-hydroxyproline1 Publication | 1 | |
Disulfide bondi | 68 ↔ 78 | By similarity | ||
Modified residuei | 80 | Glycine amide; in form delta-conotoxin PVIA1 Publication | 1 |
Post-translational modificationi
The difference between delta-conotoxin PVIA and [deamido]-delta-conotoxin PVIA lies in the state of amidation of Gly-80.
Keywords - PTMi
Amidation, Cleavage on pair of basic residues, Disulfide bond, HydroxylationExpressioni
Tissue specificityi
Expressed by the venom duct.1 Publication
Structurei
3D structure databases
ProteinModelPortali | P58913 |
SMRi | P58913 |
ModBasei | Search... |
MobiDBi | Search... |
Family & Domainsi
Domaini
The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin.By similarity
The cysteine framework is VI/VII (C-C-CC-C-C).Curated
Sequence similaritiesi
Belongs to the conotoxin O1 superfamily.Curated
Keywords - Domaini
Knottin, SignalFamily and domain databases
InterProi | View protein in InterPro IPR004214 Conotoxin IPR012322 Conotoxin_d-typ_CS |
Pfami | View protein in Pfam PF02950 Conotoxin, 1 hit |
PROSITEi | View protein in PROSITE PS60005 DELTA_CONOTOXIN, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P58913-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKLTCVMIVA VLFLTAWTFV TADDSKNGLE NHFWKARDEM KNREASKLDK
60 70 80
KEACYAPGTF CGIKPGLCCS EFCLPGVCFG G
Mass spectrometryi
Sequence databases
PIRi | A58651 |
Similar proteinsi
Cross-referencesi
Sequence databases
PIRi | A58651 |
3D structure databases
ProteinModelPortali | P58913 |
SMRi | P58913 |
ModBasei | Search... |
MobiDBi | Search... |
Protocols and materials databases
Structural Biology Knowledgebase | Search... |
Organism-specific databases
ConoServeri | 1563 PVIA precursor |
Family and domain databases
InterProi | View protein in InterPro IPR004214 Conotoxin IPR012322 Conotoxin_d-typ_CS |
Pfami | View protein in Pfam PF02950 Conotoxin, 1 hit |
PROSITEi | View protein in PROSITE PS60005 DELTA_CONOTOXIN, 1 hit |
ProtoNeti | Search... |
Entry informationi
Entry namei | O16A_CONPU | |
Accessioni | P58913Primary (citable) accession number: P58913 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 26, 2002 |
Last sequence update: | July 26, 2002 | |
Last modified: | October 10, 2018 | |
This is version 67 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Animal Toxin Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencingDocuments
- SIMILARITY comments
Index of protein domains and families