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Protein

Delta-conotoxin PVIA

Gene
N/A
Organism
Conus purpurascens (Purple cone)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Delta-conotoxins bind to site 6 of voltage-gated sodium channels (Nav) and inhibit the inactivation process. This toxin shows weak effects on rNav1.2/SCN2A (EC(50)=2.9 µM), rNav1.4/SCN4A (EC(50)=5.2 µM), hNav1.7/SCN9A (EC(50)=1.9 µM) and rNav1.7/SCN9A (EC(50)=6.4 µM) (PubMed:10627583). In vivo, this toxin shows different effects. In mice, injection of this toxin causes hyperactivity, rapid running, limb extension, and death. In fish, the peptide elicites spurts of rapid swimming, with twisted motions, quivering fins and the lockjaw extended mouth syndrome. Rigid paralysis and death are observed at higher doses. In mollusks, this peptide is inactive. Injection of this peptide together with the kappa-conotoxin PVIIA causes the sudden tetanus of prey (STOP) syndrome, which is a single, lethal "fin-pop" in envenomed fish.2 Publications

GO - Molecular functioni

Keywordsi

Molecular functionIon channel impairing toxin, Neurotoxin, Toxin, Voltage-gated sodium channel impairing toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Delta-conotoxin PVIA1 Publication
Alternative name(s):
Lockjaw peptide1 Publication
OrganismiConus purpurascens (Purple cone)
Taxonomic identifieri41690 [NCBI]
Taxonomic lineageiEukaryotaMetazoaLophotrochozoaMolluscaGastropodaCaenogastropodaNeogastropodaConoideaConidaeConusChelyconus

Organism-specific databases

ConoServeri1563 PVIA precursor

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi59T → A: No loss of activity. 1 Publication1
Mutagenesisi60F → A: Loss of biological activity. However, coinjection of this mutant protects against the wild-type conotoxin, suggesting that the mutant binds competitively to the wild-type conotoxin ligand site. Such a protective effect does not occur for all physiological targets. 1 Publication1
Mutagenesisi63I → A: Loss of activity. 1 Publication1
Mutagenesisi64K → A: No loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
PropeptideiPRO_000003489823 – 491 PublicationAdd BLAST27
PeptideiPRO_000003489952 – 80Delta-conotoxin PVIA1 PublicationAdd BLAST29

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi54 ↔ 69By similarity
Modified residuei574-hydroxyproline1 Publication1
Disulfide bondi61 ↔ 73By similarity
Modified residuei654-hydroxyproline1 Publication1
Disulfide bondi68 ↔ 78By similarity
Modified residuei80Glycine amide; in form delta-conotoxin PVIA1 Publication1

Post-translational modificationi

The difference between delta-conotoxin PVIA and [deamido]-delta-conotoxin PVIA lies in the state of amidation of Gly-80.

Keywords - PTMi

Amidation, Cleavage on pair of basic residues, Disulfide bond, Hydroxylation

Expressioni

Tissue specificityi

Expressed by the venom duct.1 Publication

Structurei

3D structure databases

ProteinModelPortaliP58913
SMRiP58913
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin.By similarity
The cysteine framework is VI/VII (C-C-CC-C-C).Curated

Sequence similaritiesi

Belongs to the conotoxin O1 superfamily.Curated

Keywords - Domaini

Knottin, Signal

Family and domain databases

InterProiView protein in InterPro
IPR004214 Conotoxin
IPR012322 Conotoxin_d-typ_CS
PfamiView protein in Pfam
PF02950 Conotoxin, 1 hit
PROSITEiView protein in PROSITE
PS60005 DELTA_CONOTOXIN, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P58913-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKLTCVMIVA VLFLTAWTFV TADDSKNGLE NHFWKARDEM KNREASKLDK
60 70 80
KEACYAPGTF CGIKPGLCCS EFCLPGVCFG G
Length:81
Mass (Da):8,945
Last modified:July 26, 2002 - v1
Checksum:iACCFE9E1C65ABFEC
GO

Mass spectrometryi

Molecular mass is 2997.3 Da from positions 52 - 80. 1 Publication

Sequence databases

PIRiA58651

Similar proteinsi

Cross-referencesi

Sequence databases

PIRiA58651

3D structure databases

ProteinModelPortaliP58913
SMRiP58913
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

ConoServeri1563 PVIA precursor

Family and domain databases

InterProiView protein in InterPro
IPR004214 Conotoxin
IPR012322 Conotoxin_d-typ_CS
PfamiView protein in Pfam
PF02950 Conotoxin, 1 hit
PROSITEiView protein in PROSITE
PS60005 DELTA_CONOTOXIN, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiO16A_CONPU
AccessioniPrimary (citable) accession number: P58913
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 26, 2002
Last sequence update: July 26, 2002
Last modified: October 10, 2018
This is version 67 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health

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