Delta-conotoxin PVIA precursor - Conus purpurascens (Purple cone)

Functionⁱ

Delta-conotoxins bind to site 6 of voltage-gated sodium channels (Nav) and inhibit the inactivation process. This toxin shows weak effects on rNav1.2/SCN2A (EC(50)=2.9 µM), rNav1.4/SCN4A (EC(50)=5.2 µM), hNav1.7/SCN9A (EC(50)=1.9 µM) and rNav1.7/SCN9A (EC(50)=6.4 µM) (PubMed:10627583). In vivo, this toxin shows different effects. In mice, injection of this toxin causes hyperactivity, rapid running, limb extension, and death. In fish, the peptide elicites spurts of rapid swimming, with twisted motions, quivering fins and the lockjaw extended mouth syndrome. Rigid paralysis and death are observed at higher doses. In mollusks, this peptide is inactive. Injection of this peptide together with the kappa-conotoxin PVIIA causes the sudden tetanus of prey (STOP) syndrome, which is a single, lethal "fin-pop" in envenomed fish.2 Publications

GO - Molecular functionⁱ

sodium channel inhibitor activity Source: InterPro
toxin activity Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Ion channel impairing toxin, Neurotoxin, Toxin, Voltage-gated sodium channel impairing toxin

Molecular function

Ion channel impairing toxin, Neurotoxin, Toxin, Voltage-gated sodium channel impairing toxin

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Delta-conotoxin PVIA1 Publication Alternative name(s): Lockjaw peptide1 Publication
Organismⁱ	Conus purpurascens (Purple cone)
Taxonomic identifierⁱ	41690 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Lophotrochozoa › Mollusca › Gastropoda › Caenogastropoda › Neogastropoda › Conoidea › Conidae › Conus › Chelyconus

Organism-specific databases

ConoServerⁱ	1563 PVIA precursor

ConoServerⁱ

1563 PVIA precursor

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Secreted

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Length
Mutagenesisⁱ	59	T → A: No loss of activity. 1 Publication	1
Mutagenesisⁱ	60	F → A: Loss of biological activity. However, coinjection of this mutant protects against the wild-type conotoxin, suggesting that the mutant binds competitively to the wild-type conotoxin ligand site. Such a protective effect does not occur for all physiological targets. 1 Publication	1
Mutagenesisⁱ	63	I → A: Loss of activity. 1 Publication	1
Mutagenesisⁱ	64	K → A: No loss of activity. 1 Publication	1

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Length
Signal peptideⁱ	1 – 22	Sequence analysisAdd BLAST	22
PropeptideⁱPRO_0000034898	23 – 49	1 PublicationAdd BLAST	27
PeptideⁱPRO_0000034899	52 – 80	Delta-conotoxin PVIA1 PublicationAdd BLAST	29

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Disulfide bondⁱ	54 ↔ 69	By similarity
Modified residueⁱ	57	4-hydroxyproline1 Publication	1
Disulfide bondⁱ	61 ↔ 73	By similarity
Modified residueⁱ	65	4-hydroxyproline1 Publication	1
Disulfide bondⁱ	68 ↔ 78	By similarity
Modified residueⁱ	80	Glycine amide; in form delta-conotoxin PVIA1 Publication	1

Post-translational modificationⁱ

The difference between delta-conotoxin PVIA and [deamido]-delta-conotoxin PVIA lies in the state of amidation of Gly-80.

Keywords - PTMⁱ

Amidation, Cleavage on pair of basic residues, Disulfide bond, Hydroxylation

Expressionⁱ

Tissue specificityⁱ

Expressed by the venom duct.1 Publication

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P58913
SMRⁱ	P58913
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domainⁱ

The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin.By similarity

The cysteine framework is VI/VII (C-C-CC-C-C).Curated

Sequence similaritiesⁱ

Belongs to the conotoxin O1 superfamily.Curated

Keywords - Domainⁱ

Knottin, Signal

Family and domain databases

InterProⁱ	View protein in InterPro IPR004214 Conotoxin IPR012322 Conotoxin_d-typ_CS
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF02950 Conotoxin, 1 hit
PROSITEⁱ	View protein in PROSITE PS60005 DELTA_CONOTOXIN, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

P58913-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MKLTCVMIVA VLFLTAWTFV TADDSKNGLE NHFWKARDEM KNREASKLDK 
        60         70         80 
KEACYAPGTF CGIKPGLCCS EFCLPGVCFG G

Length:81

Mass (Da):8,945

Last modified:July 26, 2002 - v1

Checksum:ⁱACCFE9E1C65ABFEC

GO

Mass spectrometryⁱ

Molecular mass is 2997.3 Da from positions 52 - 80. 1 Publication

Sequence databases

PIRⁱ	A58651

PIRⁱ

A58651

Similar proteinsⁱ

50% Identity

Protein	Similar proteins		Species	Score	Length	Source
P58913	Delta-conotoxin-like MVIB		CONMA		84	UniRef50_P58913
Conotoxin ViKr92		CONVR		81
Omega-conotoxin-like TxMKLT1-031		CONTE		82
Mu-conotoxin MrVIB	)	CONMR		82
Delta-conotoxin-like Ac6.1		CONAH		83
+47

Cross-referencesⁱ

Sequence databases

PIRⁱ	A58651

PIRⁱ

A58651

3D structure databases

ProteinModelPortalⁱ	P58913
SMRⁱ	P58913
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Organism-specific databases

ConoServerⁱ	1563 PVIA precursor

ConoServerⁱ

1563 PVIA precursor

Family and domain databases

InterProⁱ	View protein in InterPro IPR004214 Conotoxin IPR012322 Conotoxin_d-typ_CS
Pfamⁱ	View protein in Pfam PF02950 Conotoxin, 1 hit
PROSITEⁱ	View protein in PROSITE PS60005 DELTA_CONOTOXIN, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	O16A_CONPU
Accessionⁱ	P58913Primary (citable) accession number: P58913
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	July 26, 2002
	Last sequence update:	July 26, 2002
	Last modified:	October 10, 2018
	This is version 67 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Animal Toxin Annotation Program

UniProtKB

UniParc

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Supporting data

UniProtKB - P58913 (O16A_CONPU)

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Delta-conotoxin PVIA

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted

Extracellular region or secreted

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Family and domain databases

<p>This subsection of the ‘Sequence’ section reports information derived from mass spectrometry experiments done on the entire protein or on biologically active derived peptide(s).<p><a href='/help/mass_spectrometry' target='_top'>More...</a></p>Mass spectrometryi

Sequence databases

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Sequence databases

3D structure databases

Protocols and materials databases

Organism-specific databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

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Functionⁱ

GO - Molecular functionⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Mass spectrometryⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ