UniProtKB - P58811 (CA1A_CONTU)
Rho-conotoxin TIA
Functioni
Allosteric inhibitor of alpha-1B adrenergic receptors (ADRA1B). Binds to an allosteric modulatory site on transmembrane helix 6 and 7 at the base of extracellular loop 3 of ADRA1B (PubMed:23184947).
Also weekly inhibits alpha-1A (ADRA1A) and alpha-1D (ADRA1D) adrenergic receptors in a competive manner (PubMed:15194691).
Potently inhibits contractions of vas deferens, spleen and aorta in response to noradrenaline (PubMed:15680270).
May also inhibits nicotinic acetylcholine receptors with a possible distinct nAChR binding mode from other alpha-conotoxins, due to a different three residue motif (lacks the Ser-Xaa-Pro motif) (By similarity).
By similarity5 PublicationsMiscellaneous
GO - Molecular functioni
- acetylcholine receptor inhibitor activity Source: InterPro
- toxin activity Source: UniProtKB-KW
Keywordsi
Molecular function | Acetylcholine receptor inhibiting toxin, G-protein coupled receptor impairing toxin, Neurotoxin, Postsynaptic neurotoxin, Toxin |
Protein family/group databases
TCDBi | 8.B.32.1.9, the nicotinic acetylcholine receptor-targeting alpha-conotoxin (a-conotoxin) family |
Names & Taxonomyi
Protein namesi | Recommended name: Rho-conotoxin TIA1 PublicationShort name: Rho-TIA1 Publication |
Organismi | Conus tulipa (Fish-hunting cone snail) (Tulip cone) |
Taxonomic identifieri | 6495 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Spiralia › Lophotrochozoa › Mollusca › Gastropoda › Caenogastropoda › Neogastropoda › Conoidea › Conidae › Conus › Gastridium |
Subcellular locationi
Extracellular region or secreted
- Secreted 1 Publication
Extracellular region or secreted
- extracellular region Source: UniProtKB-SubCell
Keywords - Cellular componenti
SecretedPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 40 | N → A: Little loss in inhibition. 1 Publication | 1 | |
Mutagenesisi | 41 | W → A: Important loss in inhibition. 1 Publication | 1 | |
Mutagenesisi | 42 | R → A: Most important loss in inhibition. 1 Publication | 1 | |
Mutagenesisi | 45 | L → A: Little loss in inhibition. 1 Publication | 1 | |
Mutagenesisi | 46 | I → A: Important loss in inhibition, and change from non-competitive to competitive antagonist of alpha-1B receptor subtype. 2 Publications | 1 | |
Mutagenesisi | 50 | R → A: Little loss in inhibition. 1 Publication | 1 | |
Mutagenesisi | 56 | F → A or N: Selectivity increase for alpha-1B subtype. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 16 | Sequence analysisAdd BLAST | 16 | |
PropeptideiPRO_0000404204 | 17 – 38 | 1 PublicationAdd BLAST | 22 | |
PeptideiPRO_0000044510 | 39 – 57 | Rho-conotoxin TIA1 PublicationAdd BLAST | 19 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 43 ↔ 49 | 2 PublicationsImported | ||
Disulfide bondi | 44 ↔ 57 | 2 PublicationsImported | ||
Modified residuei | 57 | Cysteine amide1 Publication | 1 |
Keywords - PTMi
Amidation, Disulfide bondExpressioni
Tissue specificityi
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | P58811 |
BMRBi | P58811 |
SMRi | P58811 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P58811 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 40 – 42 | Interaction with ADRA1B | 3 | |
Regioni | 45 – 47 | Lacks the Ser-Xaa-Pro motif that is crucial for potent interaction with nAChRCurated | 3 |
Domaini
Sequence similaritiesi
Keywords - Domaini
SignalFamily and domain databases
InterProi | View protein in InterPro IPR009958, Conotoxin_a-typ |
Pfami | View protein in Pfam PF07365, Toxin_8, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MFTVFLLVVL ATTGVSFTLD RASDGGNAVA KKSDVTARFN WRCCLIPACR
RNHKKFCG
Sequence cautioni
Mass spectrometryi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | GQ981400 mRNA Translation: ADN79119.1 Different initiation. |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | GQ981400 mRNA Translation: ADN79119.1 Different initiation. |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1IEN | NMR | - | A | 39-57 | [»] | |
2LR9 | NMR | - | A | 39-57 | [»] | |
AlphaFoldDBi | P58811 | |||||
BMRBi | P58811 | |||||
SMRi | P58811 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein family/group databases
TCDBi | 8.B.32.1.9, the nicotinic acetylcholine receptor-targeting alpha-conotoxin (a-conotoxin) family |
Miscellaneous databases
EvolutionaryTracei | P58811 |
Family and domain databases
InterProi | View protein in InterPro IPR009958, Conotoxin_a-typ |
Pfami | View protein in Pfam PF07365, Toxin_8, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CA1A_CONTU | |
Accessioni | P58811Primary (citable) accession number: P58811 Secondary accession number(s): E2DEK8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 2, 2002 |
Last sequence update: | February 8, 2011 | |
Last modified: | May 25, 2022 | |
This is version 74 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Animal Toxin Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencingDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families