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Protein

Tropomyosin alpha-1 chain

Gene

Tpm1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments.By similarity

Miscellaneous

The sequences of cardiac and skeletal muscles are identical.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActin-binding, Muscle protein

Enzyme and pathway databases

ReactomeiR-MMU-390522 Striated Muscle Contraction
R-MMU-445355 Smooth Muscle Contraction

Names & Taxonomyi

Protein namesi
Recommended name:
Tropomyosin alpha-1 chain
Alternative name(s):
Alpha-tropomyosin
Tropomyosin-1
Gene namesi
Name:Tpm1
Synonyms:Tpm-1, Tpma
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:98809 Tpm1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002056211 – 284Tropomyosin alpha-1 chainAdd BLAST284

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei45PhosphoserineBy similarity1
Modified residuei174PhosphoserineCombined sources1
Modified residuei186PhosphoserineCombined sources1
Modified residuei206PhosphoserineCombined sources1
Modified residuei252PhosphoserineCombined sources1
Modified residuei261PhosphotyrosineBy similarity1
Modified residuei271PhosphoserineCombined sources1
Modified residuei283PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated at Ser-283 by DAPK1 in response to oxidative stress and this phosphorylation enhances stress fiber formation in endothelial cells.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PeptideAtlasiP58771
PRIDEiP58771

2D gel databases

SWISS-2DPAGEiP58771

PTM databases

iPTMnetiP58771
PhosphoSitePlusiP58771

Expressioni

Inductioni

Induced in stimulated quiescent cells.1 Publication

Gene expression databases

BgeeiENSMUSG00000032366 Expressed in 311 organ(s), highest expression level in muscle of leg
CleanExiMM_TPM1
ExpressionAtlasiP58771 baseline and differential
GenevisibleiP58771 MM

Interactioni

Subunit structurei

Homodimer. Heterodimer of an alpha (TPM1, TPM3 or TPM4) and a beta (TPM2) chain. Interacts with HRG (via the HRR domain); the interaction contributes to the antiangiogenic properties of the histidine/proline-rich region (HRR) of HRG. Interacts (via N-terminus) with LMOD2 (via N-terminus) and TMOD1 (via N-terminus).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
ACTA1P681352EBI-298478,EBI-367540From Oryctolagus cuniculus.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204291, 103 interactors
DIPiDIP-300N
IntActiP58771, 109 interactors
MINTiP58771

Structurei

3D structure databases

ProteinModelPortaliP58771
SMRiP58771
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili1 – 284By similarityAdd BLAST284

Domaini

The molecule is in a coiled coil structure that is formed by 2 polypeptide chains. The sequence exhibits a prominent seven-residues periodicity.

Sequence similaritiesi

Belongs to the tropomyosin family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

GeneTreeiENSGT00550000074494
HOGENOMiHOG000231521
HOVERGENiHBG107404
InParanoidiP58771
KOiK10373
PhylomeDBiP58771
TreeFamiTF351519

Family and domain databases

InterProiView protein in InterPro
IPR000533 Tropomyosin
PfamiView protein in Pfam
PF00261 Tropomyosin, 1 hit
PRINTSiPR00194 TROPOMYOSIN
PROSITEiView protein in PROSITE
PS00326 TROPOMYOSIN, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Note: Additional isoforms seem to exist.

This entry has 2 described isoforms and 16 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: P58771-1) [UniParc]FASTAAdd to basket
Also known as: Skeletal muscle

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MDAIKKKMQM LKLDKENALD RAEQAEADKK AAEDRSKQLE DELVSLQKKL
60 70 80 90 100
KGTEDELDKY SEALKDAQEK LELAEKKATD AEADVASLNR RIQLVEEELD
110 120 130 140 150
RAQERLATAL QKLEEAEKAA DESERGMKVI ESRAQKDEEK MEIQEIQLKE
160 170 180 190 200
AKHIAEDADR KYEEVARKLV IIESDLERAE ERAELSEGKC AELEEELKTV
210 220 230 240 250
TNNLKSLEAQ AEKYSQKEDK YEEEIKVLSD KLKEAETRAE FAERSVTKLE
260 270 280
KSIDDLEDEL YAQKLKYKAI SEELDHALND MTSI
Length:284
Mass (Da):32,681
Last modified:July 21, 1986 - v1
Checksum:iE25609F597A72F4D
GO
Isoform 2 (identifier: P58771-2) [UniParc]FASTAAdd to basket
Also known as: Fibroblast

The sequence of this isoform differs from the canonical sequence as follows:
     258-284: DELYAQKLKYKAISEELDHALNDMTSI → EKVAHAKEENLSMHQMLDQTLLELNNM

Show »
Length:284
Mass (Da):32,709
Checksum:iCD755ABFAEA04540
GO

Computationally mapped potential isoform sequencesi

There are 16 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9Q450E9Q450_MOUSE
Tropomyosin alpha-1 chain
Tpm1
284Annotation score:
E9Q452E9Q452_MOUSE
Tropomyosin alpha-1 chain
Tpm1
281Annotation score:
E9Q455E9Q455_MOUSE
Tropomyosin alpha-1 chain
Tpm1
251Annotation score:
Q8BP43Q8BP43_MOUSE
Tropomyosin 1, alpha, isoform CRA_c
Tpm1 mCG_1234
284Annotation score:
E9Q454E9Q454_MOUSE
Tropomyosin alpha-1 chain
Tpm1
287Annotation score:
E9Q448E9Q448_MOUSE
Tropomyosin alpha-1 chain
Tpm1
284Annotation score:
E9Q453E9Q453_MOUSE
Tropomyosin alpha-1 chain
Tpm1
245Annotation score:
F8WID5F8WID5_MOUSE
Tropomyosin alpha-1 chain
Tpm1
326Annotation score:
B7ZNL3B7ZNL3_MOUSE
Tpm1 protein
Tpm1 mCG_1234
284Annotation score:
G5E8R2G5E8R2_MOUSE
Tropomyosin 1, alpha, isoform CRA_k
Tpm1 mCG_1234
248Annotation score:
There are more potential isoformsShow all

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_006580258 – 284DELYA…DMTSI → EKVAHAKEENLSMHQMLDQT LLELNNM in isoform 2. 1 PublicationAdd BLAST27

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64831 mRNA Translation: CAA46043.1
M22479 mRNA Translation: AAA40483.1
CCDSiCCDS23311.1 [P58771-2]
CCDS52845.1 [P58771-1]
PIRiA31380 A60597
RefSeqiNP_001157720.1, NM_001164248.1 [P58771-1]
NP_077745.2, NM_024427.4 [P58771-2]
UniGeneiMm.121878

Genome annotation databases

EnsembliENSMUST00000113685; ENSMUSP00000109315; ENSMUSG00000032366 [P58771-1]
ENSMUST00000113707; ENSMUSP00000109337; ENSMUSG00000032366 [P58771-2]
GeneIDi22003
KEGGimmu:22003
UCSCiuc009qfq.2 mouse [P58771-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64831 mRNA Translation: CAA46043.1
M22479 mRNA Translation: AAA40483.1
CCDSiCCDS23311.1 [P58771-2]
CCDS52845.1 [P58771-1]
PIRiA31380 A60597
RefSeqiNP_001157720.1, NM_001164248.1 [P58771-1]
NP_077745.2, NM_024427.4 [P58771-2]
UniGeneiMm.121878

3D structure databases

ProteinModelPortaliP58771
SMRiP58771
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204291, 103 interactors
DIPiDIP-300N
IntActiP58771, 109 interactors
MINTiP58771

PTM databases

iPTMnetiP58771
PhosphoSitePlusiP58771

2D gel databases

SWISS-2DPAGEiP58771

Proteomic databases

PeptideAtlasiP58771
PRIDEiP58771

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000113685; ENSMUSP00000109315; ENSMUSG00000032366 [P58771-1]
ENSMUST00000113707; ENSMUSP00000109337; ENSMUSG00000032366 [P58771-2]
GeneIDi22003
KEGGimmu:22003
UCSCiuc009qfq.2 mouse [P58771-1]

Organism-specific databases

CTDi7168
MGIiMGI:98809 Tpm1

Phylogenomic databases

GeneTreeiENSGT00550000074494
HOGENOMiHOG000231521
HOVERGENiHBG107404
InParanoidiP58771
KOiK10373
PhylomeDBiP58771
TreeFamiTF351519

Enzyme and pathway databases

ReactomeiR-MMU-390522 Striated Muscle Contraction
R-MMU-445355 Smooth Muscle Contraction

Miscellaneous databases

ChiTaRSiTpm1 mouse
PROiPR:P58771
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000032366 Expressed in 311 organ(s), highest expression level in muscle of leg
CleanExiMM_TPM1
ExpressionAtlasiP58771 baseline and differential
GenevisibleiP58771 MM

Family and domain databases

InterProiView protein in InterPro
IPR000533 Tropomyosin
PfamiView protein in Pfam
PF00261 Tropomyosin, 1 hit
PRINTSiPR00194 TROPOMYOSIN
PROSITEiView protein in PROSITE
PS00326 TROPOMYOSIN, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTPM1_MOUSE
AccessioniPrimary (citable) accession number: P58771
Secondary accession number(s): P02558
, P19354, P46902, P99034
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: November 7, 2018
This is version 139 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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