UniProtKB - P58750 (PIM3_MOUSE)
Protein
Serine/threonine-protein kinase pim-3
Gene
Pim3
Organism
Mus musculus (Mouse)
Status
Functioni
Proto-oncogene with serine/threonine kinase activity that can prevent apoptosis and promote cell survival and protein translation. May contribute to tumorigenesis through: the delivery of survival signaling through phosphorylation of BAD which induces release of the anti-apoptotic protein Bcl-X(L), the regulation of cell cycle progression and protein synthesis and by regulation of MYC transcriptional activity. Additionally to this role on tumorigenesis, can also negatively regulate insulin secretion by inhibiting the activation of MAPK1/3 (ERK1/2), through SOCS6. Involved also in the control of energy metabolism and regulation of AMPK activity in modulating MYC and PPARGC1A protein levels and cell growth.3 Publications
Catalytic activityi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 69 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 170 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 46 – 54 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- protein serine/threonine kinase activity Source: UniProtKB
- protein serine kinase activity Source: UniProtKB-EC
- protein threonine kinase activity Source: UniProtKB-EC
GO - Biological processi
- apoptotic process Source: UniProtKB-KW
- cell cycle Source: UniProtKB-KW
- histone phosphorylation Source: MGI
- negative regulation of apoptotic process Source: UniProtKB
- negative regulation of insulin secretion involved in cellular response to glucose stimulus Source: UniProtKB
- protein autophosphorylation Source: MGI
- protein phosphorylation Source: UniProtKB
- regulation of mitotic cell cycle Source: UniProtKB
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Biological process | Apoptosis, Cell cycle |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Pim3 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1355297, Pim3 |
Pathology & Biotechi
Disruption phenotypei
Mice are viable and fertile, with a profound reduction in body size at birth and throughout postnatal life due to a reduction in the number of cells rather than cell size. Deficient mice have also an increased glucose tolerance.2 Publications
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 38 | K → R: Enhances insulin secretion after glucose stimulation. 1 Publication | 1 |
Keywords - Diseasei
Proto-oncogenePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000086534 | 1 – 326 | Serine/threonine-protein kinase pim-3Add BLAST | 326 |
Post-translational modificationi
Ubiquitinated, leading to proteasomal degradation.By similarity
Phosphorylated. Interaction with PPP2CA promotes dephosphorylation (By similarity).By similarity
Keywords - PTMi
Phosphoprotein, Ubl conjugationProteomic databases
PaxDbi | P58750 |
PRIDEi | P58750 |
PTM databases
iPTMneti | P58750 |
PhosphoSitePlusi | P58750 |
Expressioni
Tissue specificityi
Detected in pancreas but exclusively in beta-cells.1 Publication
Inductioni
By glucose in pancreatic-beta-cells.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000035828, Expressed in paneth cell and 302 other tissues |
ExpressionAtlasi | P58750, baseline and differential |
Genevisiblei | P58750, MM |
Interactioni
Subunit structurei
Interacts with BAD (By similarity).
Interacts with PPP2CA; this interaction promotes dephosphorylation of PIM3, ubiquitination and proteasomal degradation (By similarity).
Interacts with SOCS6.
By similarity1 PublicationProtein-protein interaction databases
BioGRIDi | 230194, 1 interactor |
STRINGi | 10090.ENSMUSP00000044603 |
Miscellaneous databases
RNActi | P58750, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 40 – 293 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 254 |
Sequence similaritiesi
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PIM subfamily.Curated
Phylogenomic databases
eggNOGi | KOG0583, Eukaryota |
GeneTreei | ENSGT00940000153394 |
HOGENOMi | CLU_000288_63_0_1 |
InParanoidi | P58750 |
OMAi | QICDHPW |
OrthoDBi | 930292at2759 |
PhylomeDBi | P58750 |
TreeFami | TF320810 |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR017348, PIM1/2/3 IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
PIRSFi | PIRSF037993, STPK_Pim-1, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
i Sequence
Sequence statusi: Complete.
P58750-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLLSKFGSLA HLCGPGGVDH LPVKILQPAK ADKESFEKVY QVGAVLGSGG
60 70 80 90 100
FGTVYAGSRI ADGLPVAVKH VVKERVTEWG SLGGVAVPLE VVLLRKVGAA
110 120 130 140 150
GGARGVIRLL DWFERPDGFL LVLERPEPAQ DLFDFITERG ALDEPLARRF
160 170 180 190 200
FAQVLAAVRH CHNCGVVHRD IKDENLLVDL RSGELKLIDF GSGAVLKDTV
210 220 230 240 250
YTDFDGTRVY SPPEWIRYHR YHGRSATVWS LGVLLYDMVC GDIPFEQDEE
260 270 280 290 300
ILRGRLFFRR RVSPECQQLI EWCLSLRPSE RPSLDQIAAH PWMLGTEGSV
310 320
PENCDLRLCA LDTDDGASTT SSSESL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC017621 mRNA Translation: AAH17621.1 BC026639 mRNA Translation: AAH26639.1 |
CCDSi | CCDS27734.1 |
RefSeqi | NP_663453.1, NM_145478.2 |
Genome annotation databases
Ensembli | ENSMUST00000042818; ENSMUSP00000044603; ENSMUSG00000035828 |
GeneIDi | 223775 |
KEGGi | mmu:223775 |
UCSCi | uc007xeq.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC017621 mRNA Translation: AAH17621.1 BC026639 mRNA Translation: AAH26639.1 |
CCDSi | CCDS27734.1 |
RefSeqi | NP_663453.1, NM_145478.2 |
3D structure databases
SMRi | P58750 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 230194, 1 interactor |
STRINGi | 10090.ENSMUSP00000044603 |
PTM databases
iPTMneti | P58750 |
PhosphoSitePlusi | P58750 |
Proteomic databases
PaxDbi | P58750 |
PRIDEi | P58750 |
Protocols and materials databases
Antibodypediai | 28269, 150 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000042818; ENSMUSP00000044603; ENSMUSG00000035828 |
GeneIDi | 223775 |
KEGGi | mmu:223775 |
UCSCi | uc007xeq.1, mouse |
Organism-specific databases
CTDi | 415116 |
MGIi | MGI:1355297, Pim3 |
Phylogenomic databases
eggNOGi | KOG0583, Eukaryota |
GeneTreei | ENSGT00940000153394 |
HOGENOMi | CLU_000288_63_0_1 |
InParanoidi | P58750 |
OMAi | QICDHPW |
OrthoDBi | 930292at2759 |
PhylomeDBi | P58750 |
TreeFami | TF320810 |
Miscellaneous databases
BioGRID-ORCSi | 223775, 2 hits in 18 CRISPR screens |
ChiTaRSi | Pim3, mouse |
PROi | PR:P58750 |
RNActi | P58750, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000035828, Expressed in paneth cell and 302 other tissues |
ExpressionAtlasi | P58750, baseline and differential |
Genevisiblei | P58750, MM |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR017348, PIM1/2/3 IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
PIRSFi | PIRSF037993, STPK_Pim-1, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PIM3_MOUSE | |
Accessioni | P58750Primary (citable) accession number: P58750 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 27, 2002 |
Last sequence update: | March 27, 2002 | |
Last modified: | February 10, 2021 | |
This is version 151 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - Human and mouse protein kinases
Human and mouse protein kinases: classification and index