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Protein

L-gulonolactone oxidase

Gene

Gulo

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Oxidizes L-gulono-1,4-lactone to hydrogen peroxide and L-xylo-hexulonolactone which spontaneously isomerizes to L-ascorbate.1 Publication

Catalytic activityi

L-gulono-1,4-lactone + O2 = L-ascorbate + H2O2.1 Publication

Cofactori

FAD1 Publication

Pathwayi: L-ascorbate biosynthesis via UDP-alpha-D-glucuronate pathway

This protein is involved in step 4 of the subpathway that synthesizes L-ascorbate from UDP-alpha-D-glucuronate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. no protein annotated in this organism
  3. Regucalcin (Rgn)
  4. L-gulonolactone oxidase (Gulo)
This subpathway is part of the pathway L-ascorbate biosynthesis via UDP-alpha-D-glucuronate pathway, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-ascorbate from UDP-alpha-D-glucuronate, the pathway L-ascorbate biosynthesis via UDP-alpha-D-glucuronate pathway and in Cofactor biosynthesis.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processAscorbate biosynthesis
LigandFAD, Flavoprotein

Enzyme and pathway databases

BRENDAi1.1.3.8 3474
UniPathwayi
UPA00991;UER00939

Names & Taxonomyi

Protein namesi
Recommended name:
L-gulonolactone oxidase (EC:1.1.3.8)
Short name:
LGO
Alternative name(s):
L-gulono-gamma-lactone oxidase
Short name:
GLO
Gene namesi
Name:Gulo
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:1353434 Gulo

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei251 – 273HelicalSequence analysisAdd BLAST23

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001281591 – 440L-gulonolactone oxidaseAdd BLAST440

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei54Pros-8alpha-FAD histidineBy similarity1

Proteomic databases

MaxQBiP58710
PaxDbiP58710
PeptideAtlasiP58710
PRIDEiP58710

PTM databases

iPTMnetiP58710
PhosphoSitePlusiP58710
SwissPalmiP58710

Expressioni

Tissue specificityi

Highly expressed in liver.1 Publication

Gene expression databases

BgeeiENSMUSG00000034450 Expressed in 47 organ(s), highest expression level in liver
CleanExiMM_GULO
GenevisibleiP58710 MM

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000060912

Structurei

3D structure databases

ProteinModelPortaliP58710
SMRiP58710
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini17 – 187FAD-binding PCMH-typePROSITE-ProRule annotationAdd BLAST171

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4730 Eukaryota
COG0277 LUCA
GeneTreeiENSGT00510000049722
HOGENOMiHOG000252847
HOVERGENiHBG005834
InParanoidiP58710
KOiK00103
OMAiSDIACTD
OrthoDBiEOG091G0FC4
TreeFamiTF328994

Family and domain databases

Gene3Di3.30.43.10, 1 hit
3.30.465.10, 1 hit
InterProiView protein in InterPro
IPR007173 ALO
IPR016166 FAD-bd_PCMH
IPR036318 FAD-bd_PCMH-like_sf
IPR016167 FAD-bd_PCMH_sub1
IPR016169 FAD-bd_PCMH_sub2
IPR010031 FAD_lactone_oxidase
IPR006094 Oxid_FAD_bind_N
IPR006093 Oxy_OxRdtase_FAD_BS
IPR030654 Sugar_lactone_oxidase
PANTHERiPTHR43762 PTHR43762, 1 hit
PfamiView protein in Pfam
PF04030 ALO, 1 hit
PF01565 FAD_binding_4, 1 hit
PIRSFiPIRSF000136 LGO_GLO, 1 hit
SUPFAMiSSF56176 SSF56176, 1 hit
TIGRFAMsiTIGR01678 FAD_lactone_ox, 1 hit
PROSITEiView protein in PROSITE
PS51387 FAD_PCMH, 1 hit
PS00862 OX2_COVAL_FAD, 1 hit

Sequencei

Sequence statusi: Complete.

P58710-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MVHGYKGVQF QNWAKTYGCS PEMYYQPTSV GEVREVLALA RQQNKKVKVV
60 70 80 90 100
GGGHSPSDIA CTDGFMIHMG KMNRVLQVDK EKKQVTVEAG ILLTDLHPQL
110 120 130 140 150
DKHGLALSNL GAVSDVTVGG VIGSGTHNTG IKHGILATQV VALTLMKADG
160 170 180 190 200
TVLECSESSN ADVFQAARVH LGCLGVILTV TLQCVPQFHL LETSFPSTLK
210 220 230 240 250
EVLDNLDSHL KKSEYFRFLW FPHSENVSII YQDHTNKEPS SASNWFWDYA
260 270 280 290 300
IGFYLLEFLL WTSTYLPRLV GWINRFFFWL LFNCKKESSN LSHKIFSYEC
310 320 330 340 350
RFKQHVQDWA IPREKTKEAL LELKAMLEAH PKVVAHYPVE VRFTRGDDIL
360 370 380 390 400
LSPCFQRDSC YMNIIMYRPY GKDVPRLDYW LAYETIMKKF GGRPHWAKAH
410 420 430 440
NCTRKDFEKM YPAFHKFCDI REKLDPTGMF LNSYLEKVFY
Length:440
Mass (Da):50,478
Last modified:July 27, 2011 - v3
Checksum:iD109B480E08E773B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti160N → K in AAH19856 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY453064 mRNA Translation: AAR15891.1
AK077740 mRNA Translation: BAC36988.1
AK167460 mRNA Translation: BAE39545.1
BC019856 mRNA Translation: AAH19856.1
BC028828 mRNA Translation: AAH28828.1
CCDSiCCDS36958.1
RefSeqiNP_848862.1, NM_178747.3
XP_006519129.1, XM_006519066.1
UniGeneiMm.26207

Genome annotation databases

EnsembliENSMUST00000059970; ENSMUSP00000060912; ENSMUSG00000034450
GeneIDi268756
KEGGimmu:268756
UCSCiuc007ujt.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY453064 mRNA Translation: AAR15891.1
AK077740 mRNA Translation: BAC36988.1
AK167460 mRNA Translation: BAE39545.1
BC019856 mRNA Translation: AAH19856.1
BC028828 mRNA Translation: AAH28828.1
CCDSiCCDS36958.1
RefSeqiNP_848862.1, NM_178747.3
XP_006519129.1, XM_006519066.1
UniGeneiMm.26207

3D structure databases

ProteinModelPortaliP58710
SMRiP58710
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000060912

PTM databases

iPTMnetiP58710
PhosphoSitePlusiP58710
SwissPalmiP58710

Proteomic databases

MaxQBiP58710
PaxDbiP58710
PeptideAtlasiP58710
PRIDEiP58710

Protocols and materials databases

DNASUi268756
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000059970; ENSMUSP00000060912; ENSMUSG00000034450
GeneIDi268756
KEGGimmu:268756
UCSCiuc007ujt.1 mouse

Organism-specific databases

CTDi268756
MGIiMGI:1353434 Gulo

Phylogenomic databases

eggNOGiKOG4730 Eukaryota
COG0277 LUCA
GeneTreeiENSGT00510000049722
HOGENOMiHOG000252847
HOVERGENiHBG005834
InParanoidiP58710
KOiK00103
OMAiSDIACTD
OrthoDBiEOG091G0FC4
TreeFamiTF328994

Enzyme and pathway databases

UniPathwayi
UPA00991;UER00939

BRENDAi1.1.3.8 3474

Miscellaneous databases

ChiTaRSiGulo mouse
PROiPR:P58710
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000034450 Expressed in 47 organ(s), highest expression level in liver
CleanExiMM_GULO
GenevisibleiP58710 MM

Family and domain databases

Gene3Di3.30.43.10, 1 hit
3.30.465.10, 1 hit
InterProiView protein in InterPro
IPR007173 ALO
IPR016166 FAD-bd_PCMH
IPR036318 FAD-bd_PCMH-like_sf
IPR016167 FAD-bd_PCMH_sub1
IPR016169 FAD-bd_PCMH_sub2
IPR010031 FAD_lactone_oxidase
IPR006094 Oxid_FAD_bind_N
IPR006093 Oxy_OxRdtase_FAD_BS
IPR030654 Sugar_lactone_oxidase
PANTHERiPTHR43762 PTHR43762, 1 hit
PfamiView protein in Pfam
PF04030 ALO, 1 hit
PF01565 FAD_binding_4, 1 hit
PIRSFiPIRSF000136 LGO_GLO, 1 hit
SUPFAMiSSF56176 SSF56176, 1 hit
TIGRFAMsiTIGR01678 FAD_lactone_ox, 1 hit
PROSITEiView protein in PROSITE
PS51387 FAD_PCMH, 1 hit
PS00862 OX2_COVAL_FAD, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiGGLO_MOUSE
AccessioniPrimary (citable) accession number: P58710
Secondary accession number(s): Q8K152
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 31, 2002
Last sequence update: July 27, 2011
Last modified: November 7, 2018
This is version 148 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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