UniProtKB - P58466 (CTDS1_MOUSE)
Protein
Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1
Gene
Ctdsp1
Organism
Mus musculus (Mouse)
Status
Functioni
Preferentially catalyzes the dephosphorylation of 'Ser-5' within the tandem 7 residue repeats in the C-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A. Negatively regulates RNA polymerase II transcription, possibly by controlling the transition from initiation/capping to processive transcript elongation (By similarity). Recruited by REST to neuronal genes that contain RE-1 elements, leading to neuronal gene silencing in non-neuronal cells.By similarity1 Publication
Catalytic activityi
Cofactori
Mg2+By similarityNote: Binds 1 Mg2+ ion per monomer.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 96 | 4-aspartylphosphate intermediateBy similarity | 1 | |
Metal bindingi | 96 | MagnesiumBy similarity | 1 | |
Active sitei | 98 | Proton donorBy similarity | 1 | |
Metal bindingi | 98 | Magnesium; via carbonyl oxygenBy similarity | 1 | |
Sitei | 152 | Transition state stabilizerBy similarity | 1 | |
Sitei | 190 | Transition state stabilizerBy similarity | 1 | |
Metal bindingi | 207 | MagnesiumBy similarity | 1 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- phosphoprotein phosphatase activity Source: GO_Central
- protein serine phosphatase activity Source: UniProtKB-EC
- protein threonine phosphatase activity Source: UniProtKB-EC
- RNA polymerase II CTD heptapeptide repeat phosphatase activity Source: MGI
GO - Biological processi
- negative regulation of G1/S transition of mitotic cell cycle Source: MGI
- negative regulation of neurogenesis Source: MGI
- negative regulation of neuron differentiation Source: MGI
- negative regulation of protein phosphorylation Source: MGI
- protein dephosphorylation Source: MGI
- regulation of transcription by RNA polymerase II Source: MGI
Keywordsi
Molecular function | Hydrolase, Protein phosphatase |
Ligand | Magnesium, Metal-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1 (EC:3.1.3.16)Alternative name(s): Golli-interacting protein Short name: GIP Nuclear LIM interactor-interacting factor 3 Short name: NLI-interacting factor 3 Small C-terminal domain phosphatase 1 Short name: SCP1 Short name: Small CTD phosphatase 1 |
Gene namesi | Name:Ctdsp1 Synonyms:Nif3, Nliif |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2654470, Ctdsp1 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Nucleus
- nucleoplasm Source: MGI
- nucleus Source: MGI
Keywords - Cellular componenti
NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 96 | D → E: Leads to enhanced neuronal differentiation of stem cells; when associated with N-98. 1 Publication | 1 | |
Mutagenesisi | 98 | D → N: Leads to enhanced neuronal differentiation of stem cells; when associated with E-96. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000212573 | 1 – 261 | Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1Add BLAST | 261 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 |
Keywords - PTMi
AcetylationProteomic databases
EPDi | P58466 |
jPOSTi | P58466 |
PaxDbi | P58466 |
PeptideAtlasi | P58466 |
PRIDEi | P58466 |
ProteomicsDBi | 283976 |
PTM databases
iPTMneti | P58466 |
PhosphoSitePlusi | P58466 |
SwissPalmi | P58466 |
Expressioni
Tissue specificityi
Expression is restricted to non-neuronal tissues.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000026176, Expressed in granulocyte and 292 other tissues |
Genevisiblei | P58466, MM |
Interactioni
Subunit structurei
Monomer.
Interacts with REST.
By similarityBinary interactionsi
P58466
With | #Exp. | IntAct |
---|---|---|
CTDSP1 [Q9GZU7] from Homo sapiens. | 2 | EBI-7091612,EBI-751587 |
Protein-protein interaction databases
BioGRIDi | 230610, 1 interactor |
IntActi | P58466, 2 interactors |
MINTi | P58466 |
STRINGi | 10090.ENSMUSP00000027367 |
Miscellaneous databases
RNActi | P58466, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 86 – 244 | FCP1 homologyPROSITE-ProRule annotationAdd BLAST | 159 |
Phylogenomic databases
eggNOGi | KOG1605, Eukaryota |
GeneTreei | ENSGT01020000230486 |
HOGENOMi | CLU_020262_4_0_1 |
InParanoidi | P58466 |
OMAi | CCLCHDE |
OrthoDBi | 1176152at2759 |
PhylomeDBi | P58466 |
TreeFami | TF313556 |
Family and domain databases
Gene3Di | 3.40.50.1000, 1 hit |
InterProi | View protein in InterPro IPR011948, Dullard_phosphatase IPR004274, FCP1_dom IPR036412, HAD-like_sf IPR023214, HAD_sf IPR040078, RNA_Pol_CTD_Phosphatase |
Pfami | View protein in Pfam PF03031, NIF, 1 hit |
SFLDi | SFLDG01124, C0.1:_RNA_Pol_CTD_Phosphatase, 1 hit |
SMARTi | View protein in SMART SM00577, CPDc, 1 hit |
SUPFAMi | SSF56784, SSF56784, 1 hit |
TIGRFAMsi | TIGR02251, HIF-SF_euk, 1 hit |
PROSITEi | View protein in PROSITE PS50969, FCP1, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
P58466-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDSSAVITQI SKEEARGPLR GKGDQKSAVS QKPRSRGILH SLFCCVCRDD
60 70 80 90 100
GEPLPAHSGA PLLVEENGAI PKHTPVQYLL PEAKAQDSDK ICVVIDLDET
110 120 130 140 150
LVHSSFKPVN NADFIIPVEI DGVVHQVYVL KRPHVDEFLQ RMGELFECVL
160 170 180 190 200
FTASLAKYAD PVADLLDKWG AFRARLFRES CVFHRGNYVK DLSRLGRDLR
210 220 230 240 250
RVLILDNSPA SYVFHPDNAV PVASWFDNMS DTELHDLLPF FEQLSRVDDV
260
YSVLRQPRPG S
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketF7AIT6 | F7AIT6_MOUSE | Carboxy-terminal domain RNA polymer... | Ctdsp1 | 131 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY028804 mRNA Translation: AAK83555.1 BC065158 mRNA Translation: AAH65158.1 BC079638 mRNA Translation: AAH79638.1 |
CCDSi | CCDS15048.1 |
RefSeqi | NP_694728.1, NM_153088.2 |
Genome annotation databases
Ensembli | ENSMUST00000027367; ENSMUSP00000027367; ENSMUSG00000026176 |
GeneIDi | 227292 |
KEGGi | mmu:227292 |
UCSCi | uc007blz.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY028804 mRNA Translation: AAK83555.1 BC065158 mRNA Translation: AAH65158.1 BC079638 mRNA Translation: AAH79638.1 |
CCDSi | CCDS15048.1 |
RefSeqi | NP_694728.1, NM_153088.2 |
3D structure databases
SMRi | P58466 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 230610, 1 interactor |
IntActi | P58466, 2 interactors |
MINTi | P58466 |
STRINGi | 10090.ENSMUSP00000027367 |
PTM databases
iPTMneti | P58466 |
PhosphoSitePlusi | P58466 |
SwissPalmi | P58466 |
Proteomic databases
EPDi | P58466 |
jPOSTi | P58466 |
PaxDbi | P58466 |
PeptideAtlasi | P58466 |
PRIDEi | P58466 |
ProteomicsDBi | 283976 |
Protocols and materials databases
Antibodypediai | 34272, 401 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000027367; ENSMUSP00000027367; ENSMUSG00000026176 |
GeneIDi | 227292 |
KEGGi | mmu:227292 |
UCSCi | uc007blz.2, mouse |
Organism-specific databases
CTDi | 58190 |
MGIi | MGI:2654470, Ctdsp1 |
Phylogenomic databases
eggNOGi | KOG1605, Eukaryota |
GeneTreei | ENSGT01020000230486 |
HOGENOMi | CLU_020262_4_0_1 |
InParanoidi | P58466 |
OMAi | CCLCHDE |
OrthoDBi | 1176152at2759 |
PhylomeDBi | P58466 |
TreeFami | TF313556 |
Miscellaneous databases
BioGRID-ORCSi | 227292, 2 hits in 52 CRISPR screens |
ChiTaRSi | Ctdsp1, mouse |
PROi | PR:P58466 |
RNActi | P58466, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000026176, Expressed in granulocyte and 292 other tissues |
Genevisiblei | P58466, MM |
Family and domain databases
Gene3Di | 3.40.50.1000, 1 hit |
InterProi | View protein in InterPro IPR011948, Dullard_phosphatase IPR004274, FCP1_dom IPR036412, HAD-like_sf IPR023214, HAD_sf IPR040078, RNA_Pol_CTD_Phosphatase |
Pfami | View protein in Pfam PF03031, NIF, 1 hit |
SFLDi | SFLDG01124, C0.1:_RNA_Pol_CTD_Phosphatase, 1 hit |
SMARTi | View protein in SMART SM00577, CPDc, 1 hit |
SUPFAMi | SSF56784, SSF56784, 1 hit |
TIGRFAMsi | TIGR02251, HIF-SF_euk, 1 hit |
PROSITEi | View protein in PROSITE PS50969, FCP1, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CTDS1_MOUSE | |
Accessioni | P58466Primary (citable) accession number: P58466 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 13, 2001 |
Last sequence update: | December 13, 2001 | |
Last modified: | April 7, 2021 | |
This is version 140 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot