UniProtKB - P58465 (CTDSL_MOUSE)
Protein
CTD small phosphatase-like protein
Gene
Ctdspl
Organism
Mus musculus (Mouse)
Status
Functioni
Preferentially catalyzes the dephosphorylation of 'Ser-5' within the tandem 7 residue repeats in the C-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A. Negatively regulates RNA polymerase II transcription, possibly by controlling the transition from initiation/capping to processive transcript elongation. Recruited by REST to neuronal genes that contain RE-1 elements, leading to neuronal gene silencing in non-neuronal cells (By similarity).By similarity
Catalytic activityi
Cofactori
Mg2+By similarityNote: Binds 1 Mg2+ ion per monomer.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 112 | 4-aspartylphosphate intermediateBy similarity | 1 | |
Metal bindingi | 112 | MagnesiumBy similarity | 1 | |
Active sitei | 114 | Proton donorBy similarity | 1 | |
Metal bindingi | 114 | Magnesium; via carbonyl oxygenBy similarity | 1 | |
Sitei | 168 | Transition state stabilizerBy similarity | 1 | |
Sitei | 206 | Transition state stabilizerBy similarity | 1 | |
Metal bindingi | 223 | MagnesiumBy similarity | 1 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- phosphoprotein phosphatase activity Source: GO_Central
- protein serine phosphatase activity Source: UniProtKB-EC
- protein threonine phosphatase activity Source: UniProtKB-EC
- RNA polymerase II CTD heptapeptide repeat phosphatase activity Source: GO_Central
GO - Biological processi
- negative regulation of G1/S transition of mitotic cell cycle Source: MGI
- negative regulation of protein phosphorylation Source: MGI
Keywordsi
Molecular function | Hydrolase, Protein phosphatase |
Ligand | Magnesium, Metal-binding |
Names & Taxonomyi
Protein namesi | Recommended name: CTD small phosphatase-like protein (EC:3.1.3.16)Short name: CTDSP-like Alternative name(s): Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 3 NIF-like protein Nuclear LIM interactor-interacting factor 1 Short name: NLI-interacting factor 1 Small C-terminal domain phosphatase 3 Short name: SCP3 Short name: Small CTD phosphatase 3 |
Gene namesi | Name:Ctdspl Synonyms:Nif1, Nifl |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1916524, Ctdspl |
Subcellular locationi
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000212570 | 1 – 276 | CTD small phosphatase-like proteinAdd BLAST | 276 |
Proteomic databases
jPOSTi | P58465 |
PaxDbi | P58465 |
PRIDEi | P58465 |
PTM databases
iPTMneti | P58465 |
PhosphoSitePlusi | P58465 |
SwissPalmi | P58465 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000047409, Expressed in cardiac ventricle and 257 other tissues |
ExpressionAtlasi | P58465, baseline and differential |
Genevisiblei | P58465, MM |
Interactioni
Subunit structurei
Monomer.
Interacts with REST.
By similarityProtein-protein interaction databases
STRINGi | 10090.ENSMUSP00000072852 |
Miscellaneous databases
RNActi | P58465, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 102 – 260 | FCP1 homologyPROSITE-ProRule annotationAdd BLAST | 159 |
Phylogenomic databases
eggNOGi | KOG1605, Eukaryota |
GeneTreei | ENSGT00990000203506 |
InParanoidi | P58465 |
OrthoDBi | 1176152at2759 |
TreeFami | TF313556 |
Family and domain databases
Gene3Di | 3.40.50.1000, 1 hit |
InterProi | View protein in InterPro IPR011948, Dullard_phosphatase IPR004274, FCP1_dom IPR036412, HAD-like_sf IPR023214, HAD_sf IPR040078, RNA_Pol_CTD_Phosphatase |
Pfami | View protein in Pfam PF03031, NIF, 1 hit |
SFLDi | SFLDG01124, C0.1:_RNA_Pol_CTD_Phosphatase, 1 hit |
SMARTi | View protein in SMART SM00577, CPDc, 1 hit |
SUPFAMi | SSF56784, SSF56784, 1 hit |
TIGRFAMsi | TIGR02251, HIF-SF_euk, 1 hit |
PROSITEi | View protein in PROSITE PS50969, FCP1, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
P58465-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDGPAIITQV TNPKEDEARS PVAGEKASQR NISLKKQRGR SILSSFFCCF
60 70 80 90 100
RDYNVEAPPA NSPSVLPPLV EENGGLQKGD QRQVIPVPSP PAKYLLPEVT
110 120 130 140 150
VLDYGKKCVV IDLDETLVHS SFKPISNADF IVPVEIDGTI HQVYVLKRPH
160 170 180 190 200
VDEFLQRMGQ LFECVLFTAS LAKYADPVAD LLDRWGVFRA RLFRESCVFH
210 220 230 240 250
RGNYVKDLSR LGRELSKVII VDNSPASYIF HPENAVPVQS WFDDMTDTEL
260 270
LDLIPFFEGL SREDDVYSML HRLCSR
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketG3UXN0 | G3UXN0_MOUSE | Protein-serine/threonine phosphatas... | Ctdspl | 265 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 37 | Q → E in CAC69078 (Ref. 1) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ344340 mRNA Translation: CAC69078.2 AC055818 Genomic DNA No translation available. AC156800 Genomic DNA No translation available. BC094289 mRNA Translation: AAH94289.1 |
CCDSi | CCDS23605.1 |
RefSeqi | NP_598471.3, NM_133710.3 |
Genome annotation databases
Ensembli | ENSMUST00000073109; ENSMUSP00000072852; ENSMUSG00000047409 |
GeneIDi | 69274 |
KEGGi | mmu:69274 |
UCSCi | uc009rzz.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ344340 mRNA Translation: CAC69078.2 AC055818 Genomic DNA No translation available. AC156800 Genomic DNA No translation available. BC094289 mRNA Translation: AAH94289.1 |
CCDSi | CCDS23605.1 |
RefSeqi | NP_598471.3, NM_133710.3 |
3D structure databases
SMRi | P58465 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000072852 |
PTM databases
iPTMneti | P58465 |
PhosphoSitePlusi | P58465 |
SwissPalmi | P58465 |
Proteomic databases
jPOSTi | P58465 |
PaxDbi | P58465 |
PRIDEi | P58465 |
Protocols and materials databases
Antibodypediai | 28378, 182 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000073109; ENSMUSP00000072852; ENSMUSG00000047409 |
GeneIDi | 69274 |
KEGGi | mmu:69274 |
UCSCi | uc009rzz.2, mouse |
Organism-specific databases
CTDi | 10217 |
MGIi | MGI:1916524, Ctdspl |
Phylogenomic databases
eggNOGi | KOG1605, Eukaryota |
GeneTreei | ENSGT00990000203506 |
InParanoidi | P58465 |
OrthoDBi | 1176152at2759 |
TreeFami | TF313556 |
Miscellaneous databases
BioGRID-ORCSi | 69274, 0 hits in 18 CRISPR screens |
ChiTaRSi | Ctdspl, mouse |
PROi | PR:P58465 |
RNActi | P58465, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000047409, Expressed in cardiac ventricle and 257 other tissues |
ExpressionAtlasi | P58465, baseline and differential |
Genevisiblei | P58465, MM |
Family and domain databases
Gene3Di | 3.40.50.1000, 1 hit |
InterProi | View protein in InterPro IPR011948, Dullard_phosphatase IPR004274, FCP1_dom IPR036412, HAD-like_sf IPR023214, HAD_sf IPR040078, RNA_Pol_CTD_Phosphatase |
Pfami | View protein in Pfam PF03031, NIF, 1 hit |
SFLDi | SFLDG01124, C0.1:_RNA_Pol_CTD_Phosphatase, 1 hit |
SMARTi | View protein in SMART SM00577, CPDc, 1 hit |
SUPFAMi | SSF56784, SSF56784, 1 hit |
TIGRFAMsi | TIGR02251, HIF-SF_euk, 1 hit |
PROSITEi | View protein in PROSITE PS50969, FCP1, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CTDSL_MOUSE | |
Accessioni | P58465Primary (citable) accession number: P58465 Secondary accession number(s): Q52KL5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 13, 2001 |
Last sequence update: | October 3, 2012 | |
Last modified: | February 10, 2021 | |
This is version 120 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot