UniProtKB - P58399 (PA2H1_BOTPI)
Protein
Basic phospholipase A2 homolog piratoxin-1
Gene
N/A
Organism
Bothrops pirajai (Piraja's lance0 head)
Status
Functioni
Snake venom phospholipase A2 (PLA2) homolog that lacks enzymatic activity, but displays myotoxin and edema-inducing activities in vivo. In vitro neuromuscular activities have also been observed, but they are not found in vivo and can be explained by the destabilization of the muscle membrane by the toxin. The myotoxic activity is inhibited by rosmarinic acid (RA).2 Publications
Caution
Does not bind calcium as one of the calcium-binding sites is lost (Asp->Lys in position 48, which corresponds to 'Lys-49' in the current nomenclature).Curated
GO - Molecular functioni
- calcium ion binding Source: InterPro
- phospholipase A2 activity Source: InterPro
- toxin activity Source: UniProtKB-KW
GO - Biological processi
- arachidonic acid secretion Source: InterPro
- lipid catabolic process Source: InterPro
- phospholipid metabolic process Source: InterPro
Keywordsi
Molecular function | Myotoxin, Toxin |
Enzyme and pathway databases
BRENDAi | 3.1.1.4 8187 |
Names & Taxonomyi
Protein namesi | Recommended name: Basic phospholipase A2 homolog piratoxin-1Short name: svPLA2 homolog Alternative name(s): Myotoxin SIV-SP5 Piratoxin-I Short name: PrTX-I |
Organismi | Bothrops pirajai (Piraja's lance0 head) |
Taxonomic identifieri | 113192 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Lepidosauria › Squamata › Bifurcata › Unidentata › Episquamata › Toxicofera › Serpentes › Colubroidea › Viperidae › Crotalinae › Bothrops |
Subcellular locationi
Extracellular region or secreted
Extracellular region or secreted
- extracellular region Source: UniProtKB-SubCell
Keywords - Cellular componenti
SecretedPathology & Biotechi
Toxic dosei
LD50 is 8 mg/kg by intraperitoneal injection into mice.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000161627 | 1 – 121 | Basic phospholipase A2 homolog piratoxin-1Add BLAST | 121 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 26 ↔ 115 | Combined sources1 Publication | ||
Disulfide bondi | 28 ↔ 44 | Combined sources1 Publication | ||
Disulfide bondi | 43 ↔ 95 | Combined sources1 Publication | ||
Disulfide bondi | 49 ↔ 121 | Combined sources1 Publication | ||
Disulfide bondi | 50 ↔ 88 | Combined sources1 Publication | ||
Disulfide bondi | 57 ↔ 81 | Combined sources1 Publication | ||
Disulfide bondi | 75 ↔ 86 | Combined sources1 Publication |
Keywords - PTMi
Disulfide bondExpressioni
Tissue specificityi
Expressed by the venom gland.
Interactioni
Subunit structurei
Homodimer.2 Publications
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2OK9 | X-ray | 2.34 | A/B | 1-121 | [»] | |
3QNL | X-ray | 1.77 | A/B | 1-121 | [»] | |
4YU7 | X-ray | 1.65 | A/B | 1-121 | [»] | |
4YZ7 | X-ray | 1.96 | A/B | 1-121 | [»] | |
ProteinModelPortali | P58399 | |||||
SMRi | P58399 | |||||
ModBasei | Search... | |||||
MobiDBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P58399 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
HOVERGENi | HBG008137 |
Family and domain databases
CDDi | cd00125 PLA2c, 1 hit |
Gene3Di | 1.20.90.10, 1 hit |
InterProi | View protein in InterPro IPR001211 PLipase_A2 IPR033112 PLipase_A2_Asp_AS IPR016090 PLipase_A2_dom IPR036444 PLipase_A2_dom_sf IPR033113 PLipase_A2_His_AS |
PANTHERi | PTHR11716 PTHR11716, 1 hit |
Pfami | View protein in Pfam PF00068 Phospholip_A2_1, 1 hit |
PRINTSi | PR00389 PHPHLIPASEA2 |
SMARTi | View protein in SMART SM00085 PA2c, 1 hit |
SUPFAMi | SSF48619 SSF48619, 1 hit |
PROSITEi | View protein in PROSITE PS00119 PA2_ASP, 1 hit PS00118 PA2_HIS, 1 hit |
i Sequence
Sequence statusi: Complete.
P58399-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
SLFELGKMIL QETGKNPAKS YGAYGCNCGV LGRGKPKDAT DRCCYVHKCC
60 70 80 90 100
YKKLTGCNPK KDRYSYSWKD KTIVCGENNP CLKELCECDK AVAICLRENL
110 120
GTYNKLYRYH LKPFCKKADD C
Similar proteinsi
Cross-referencesi
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2OK9 | X-ray | 2.34 | A/B | 1-121 | [»] | |
3QNL | X-ray | 1.77 | A/B | 1-121 | [»] | |
4YU7 | X-ray | 1.65 | A/B | 1-121 | [»] | |
4YZ7 | X-ray | 1.96 | A/B | 1-121 | [»] | |
ProteinModelPortali | P58399 | |||||
SMRi | P58399 | |||||
ModBasei | Search... | |||||
MobiDBi | Search... |
Protocols and materials databases
Structural Biology Knowledgebase | Search... |
Phylogenomic databases
HOVERGENi | HBG008137 |
Enzyme and pathway databases
BRENDAi | 3.1.1.4 8187 |
Miscellaneous databases
EvolutionaryTracei | P58399 |
Family and domain databases
CDDi | cd00125 PLA2c, 1 hit |
Gene3Di | 1.20.90.10, 1 hit |
InterProi | View protein in InterPro IPR001211 PLipase_A2 IPR033112 PLipase_A2_Asp_AS IPR016090 PLipase_A2_dom IPR036444 PLipase_A2_dom_sf IPR033113 PLipase_A2_His_AS |
PANTHERi | PTHR11716 PTHR11716, 1 hit |
Pfami | View protein in Pfam PF00068 Phospholip_A2_1, 1 hit |
PRINTSi | PR00389 PHPHLIPASEA2 |
SMARTi | View protein in SMART SM00085 PA2c, 1 hit |
SUPFAMi | SSF48619 SSF48619, 1 hit |
PROSITEi | View protein in PROSITE PS00119 PA2_ASP, 1 hit PS00118 PA2_HIS, 1 hit |
ProtoNeti | Search... |
Entry informationi
Entry namei | PA2H1_BOTPI | |
Accessioni | P58399Primary (citable) accession number: P58399 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 16, 2001 |
Last sequence update: | December 5, 2001 | |
Last modified: | December 20, 2017 | |
This is version 82 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Animal Toxin Annotation Program | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencingDocuments
- SIMILARITY comments
Index of protein domains and families - PDB cross-references
Index of Protein Data Bank (PDB) cross-references