UniProtKB - P58308 (OX2R_MOUSE)
Protein
Orexin receptor type 2
Gene
Hcrtr2
Organism
Mus musculus (Mouse)
Status
Functioni
Nonselective, high-affinity receptor for both orexin-A and orexin-B neuropeptides. Triggers an increase in cytoplasmic Ca2+ levels in response to orexin-A binding.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 44 | Important for responses to orexinBy similarity | 1 | |
Binding sitei | 324 | AgonistBy similarity | 1 |
GO - Molecular functioni
- G protein-coupled receptor activity Source: MGI
- orexin receptor activity Source: MGI
- peptide hormone binding Source: MGI
GO - Biological processi
- circadian sleep/wake cycle process Source: InterPro
- feeding behavior Source: MGI
- G protein-coupled receptor signaling pathway Source: MGI
- neuropeptide signaling pathway Source: UniProtKB
- phospholipase C-activating G protein-coupled receptor signaling pathway Source: MGI
- regulation of circadian sleep/wake cycle, wakefulness Source: UniProtKB
- regulation of cytosolic calcium ion concentration Source: UniProtKB
Keywordsi
Molecular function | G-protein coupled receptor, Receptor, Transducer |
Enzyme and pathway databases
Reactomei | R-MMU-389397, Orexin and neuropeptides FF and QRFP bind to their respective receptors R-MMU-416476, G alpha (q) signalling events |
Names & Taxonomyi
Protein namesi | Recommended name: Orexin receptor type 2Short name: Ox-2-R Short name: Ox2-R Short name: Ox2R Alternative name(s): Hypocretin receptor type 2 |
Gene namesi | Name:Hcrtr2 Synonyms:Mox2r |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2680765, Hcrtr2 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Multi-pass membrane protein By similarity
Plasma Membrane
- integral component of plasma membrane Source: UniProtKB
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 54 | ExtracellularBy similarityAdd BLAST | 54 | |
Transmembranei | 55 – 75 | Helical; Name=1By similarityAdd BLAST | 21 | |
Topological domaini | 76 – 88 | CytoplasmicBy similarityAdd BLAST | 13 | |
Transmembranei | 89 – 110 | Helical; Name=2By similarityAdd BLAST | 22 | |
Topological domaini | 111 – 127 | ExtracellularBy similarityAdd BLAST | 17 | |
Transmembranei | 128 – 150 | Helical; Name=3By similarityAdd BLAST | 23 | |
Topological domaini | 151 – 170 | CytoplasmicBy similarityAdd BLAST | 20 | |
Transmembranei | 171 – 191 | Helical; Name=4By similarityAdd BLAST | 21 | |
Topological domaini | 192 – 222 | ExtracellularBy similarityAdd BLAST | 31 | |
Transmembranei | 223 – 243 | Helical; Name=5By similarityAdd BLAST | 21 | |
Topological domaini | 244 – 304 | CytoplasmicBy similarityAdd BLAST | 61 | |
Transmembranei | 305 – 326 | Helical; Name=6By similarityAdd BLAST | 22 | |
Topological domaini | 327 – 342 | ExtracellularBy similarityAdd BLAST | 16 | |
Transmembranei | 343 – 366 | Helical; Name=7By similarityAdd BLAST | 24 | |
Topological domaini | 367 – 460 | CytoplasmicBy similarityAdd BLAST | 94 |
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000069990 | 1 – 460 | Orexin receptor type 2Add BLAST | 460 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 14 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 22 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 127 ↔ 210 | By similarity | ||
Glycosylationi | 202 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
PaxDbi | P58308 |
PRIDEi | P58308 |
PTM databases
GlyGeni | P58308, 3 sites |
PhosphoSitePlusi | P58308 |
SwissPalmi | P58308 |
Expressioni
Tissue specificityi
Widely expressed. Isoform 2 not detected in skeletal muscle and kidney.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000032360, Expressed in brain and 41 other tissues |
Genevisiblei | P58308, MM |
Interactioni
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000058230 |
Chemistry databases
BindingDBi | P58308 |
Miscellaneous databases
RNActi | P58308, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 33 – 49 | Required for response to orexin-ABy similarityAdd BLAST | 17 |
Domaini
The N-terminal region is required for orexin signaling.By similarity
Sequence similaritiesi
Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3656, Eukaryota |
GeneTreei | ENSGT01010000222375 |
HOGENOMi | CLU_009579_6_3_1 |
InParanoidi | P58308 |
OMAi | IRSHHDE |
OrthoDBi | 981089at2759 |
PhylomeDBi | P58308 |
TreeFami | TF315303 |
Family and domain databases
InterProi | View protein in InterPro IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM IPR000204, Orexin_rcpt IPR004060, Orexin_rcpt_2 |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit PF03827, Orexin_rec2, 1 hit |
PRINTSi | PR00237, GPCRRHODOPSN PR01522, OREXIN2R PR01064, OREXINR |
SMARTi | View protein in SMART SM01381, 7TM_GPCR_Srsx, 1 hit |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketIsoform 1 (identifier: P58308-1) [UniParc]FASTAAdd to basket
Also known as: 2b
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSSTKLEDSL SRRNWSSASE LNETQEPFLN PTDYDDEEFL RYLWREYLHP
60 70 80 90 100
KEYEWVLIAG YIIVFVVALI GNVLVCVAVW KNHHMRTVTN YFIVNLSLAD
110 120 130 140 150
VLVTITCLPA TLVVDITETW FFGQSLCKVI PYLQTVSVSV SVLTLSCIAL
160 170 180 190 200
DRWYAICHPL MFKSTAKRAR NSIVVIWIVS CIIMIPQAIV MECSSMLPGL
210 220 230 240 250
ANKTTLFTVC DEHWGGEVYP KMYHICFFLV TYMAPLCLMI LAYLQIFRKL
260 270 280 290 300
WCRQIPGTSS VVQRKWKQQQ PVSQPRGSGQ QSKARISAVA AEIKQIRARR
310 320 330 340 350
KTARMLMVVL LVFAICYLPI SILNVLKRVF GMFTHTEDRE TVYAWFTFSH
360 370 380 390 400
WLVYANSAAN PIIYNFLSGK FREEFKAAFS CCLGVHHRQG DRLARGRTST
410 420 430 440 450
ESRKSLTTQI SNFDNVSKLS EHVVLTSIST LPAANGAGPL QNWYLQQGVP
460
SSLLSTWLEV
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 201 | A → T in AAK71327 (Ref. 3) Curated | 1 | |
Sequence conflicti | 240 | I → V in AAK71327 (Ref. 3) Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_016466 | 444 – 460 | Missing in isoform 2. 1 PublicationAdd BLAST | 17 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY336084 mRNA Translation: AAR01327.1 AY336085 mRNA Translation: AAR01328.1 AY339390 , AY339383, AY339384, AY339385, AY339386, AY339387, AY339388, AY339389 Genomic DNA Translation: AAR11293.1 AY339389 , AY339383, AY339384, AY339385, AY339386, AY339387, AY339388 Genomic DNA Translation: AAR11294.1 AK038551 mRNA Translation: BAC30039.1 AK048781 mRNA Translation: BAC33457.1 AF394597 mRNA Translation: AAK71327.1 |
CCDSi | CCDS23350.1 [P58308-1] CCDS90628.1 [P58308-2] |
RefSeqi | NP_945200.1, NM_198962.3 [P58308-1] |
Genome annotation databases
Ensembli | ENSMUST00000063140; ENSMUSP00000058230; ENSMUSG00000032360 [P58308-1] ENSMUST00000184757; ENSMUSP00000139377; ENSMUSG00000032360 [P58308-2] |
GeneIDi | 387285 |
KEGGi | mmu:387285 |
UCSCi | uc009qsz.1, mouse [P58308-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY336084 mRNA Translation: AAR01327.1 AY336085 mRNA Translation: AAR01328.1 AY339390 , AY339383, AY339384, AY339385, AY339386, AY339387, AY339388, AY339389 Genomic DNA Translation: AAR11293.1 AY339389 , AY339383, AY339384, AY339385, AY339386, AY339387, AY339388 Genomic DNA Translation: AAR11294.1 AK038551 mRNA Translation: BAC30039.1 AK048781 mRNA Translation: BAC33457.1 AF394597 mRNA Translation: AAK71327.1 |
CCDSi | CCDS23350.1 [P58308-1] CCDS90628.1 [P58308-2] |
RefSeqi | NP_945200.1, NM_198962.3 [P58308-1] |
3D structure databases
SMRi | P58308 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000058230 |
Chemistry databases
BindingDBi | P58308 |
ChEMBLi | CHEMBL2434818 |
DrugCentrali | P58308 |
Protein family/group databases
GPCRDBi | Search... |
PTM databases
GlyGeni | P58308, 3 sites |
PhosphoSitePlusi | P58308 |
SwissPalmi | P58308 |
Proteomic databases
PaxDbi | P58308 |
PRIDEi | P58308 |
Protocols and materials databases
Antibodypediai | 17332, 360 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000063140; ENSMUSP00000058230; ENSMUSG00000032360 [P58308-1] ENSMUST00000184757; ENSMUSP00000139377; ENSMUSG00000032360 [P58308-2] |
GeneIDi | 387285 |
KEGGi | mmu:387285 |
UCSCi | uc009qsz.1, mouse [P58308-1] |
Organism-specific databases
CTDi | 3062 |
MGIi | MGI:2680765, Hcrtr2 |
Phylogenomic databases
eggNOGi | KOG3656, Eukaryota |
GeneTreei | ENSGT01010000222375 |
HOGENOMi | CLU_009579_6_3_1 |
InParanoidi | P58308 |
OMAi | IRSHHDE |
OrthoDBi | 981089at2759 |
PhylomeDBi | P58308 |
TreeFami | TF315303 |
Enzyme and pathway databases
Reactomei | R-MMU-389397, Orexin and neuropeptides FF and QRFP bind to their respective receptors R-MMU-416476, G alpha (q) signalling events |
Miscellaneous databases
BioGRID-ORCSi | 387285, 2 hits in 14 CRISPR screens |
ChiTaRSi | Hcrtr2, mouse |
PROi | PR:P58308 |
RNActi | P58308, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000032360, Expressed in brain and 41 other tissues |
Genevisiblei | P58308, MM |
Family and domain databases
InterProi | View protein in InterPro IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM IPR000204, Orexin_rcpt IPR004060, Orexin_rcpt_2 |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit PF03827, Orexin_rec2, 1 hit |
PRINTSi | PR00237, GPCRRHODOPSN PR01522, OREXIN2R PR01064, OREXINR |
SMARTi | View protein in SMART SM01381, 7TM_GPCR_Srsx, 1 hit |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | OX2R_MOUSE | |
Accessioni | P58308Primary (citable) accession number: P58308 Secondary accession number(s): Q5SGC8, Q6VLX3, Q8BG12 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 26, 2001 |
Last sequence update: | May 30, 2003 | |
Last modified: | December 2, 2020 | |
This is version 149 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - 7-transmembrane G-linked receptors
List of 7-transmembrane G-linked receptor entries