UniProtKB - P58307 (OX1R_MOUSE)
Protein
Orexin receptor type 1
Gene
Hcrtr1
Organism
Mus musculus (Mouse)
Status
Functioni
Moderately selective excitatory receptor for orexin-A and, with a lower affinity, for orexin-B neuropeptide. Triggers an increase in cytoplasmic Ca2+ levels in response to orexin-A binding.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 36 | Important for responses to orexinBy similarity | 1 | |
Binding sitei | 318 | AgonistBy similarity | 1 |
GO - Molecular functioni
- G protein-coupled receptor activity Source: GO_Central
- orexin receptor activity Source: UniProtKB
- peptide hormone binding Source: MGI
GO - Biological processi
- feeding behavior Source: InterPro
- G protein-coupled receptor signaling pathway Source: MGI
- neuropeptide signaling pathway Source: UniProtKB
- positive regulation of ERK1 and ERK2 cascade Source: MGI
- regulation of cytosolic calcium ion concentration Source: UniProtKB
Keywordsi
Molecular function | G-protein coupled receptor, Receptor, Transducer |
Enzyme and pathway databases
Reactomei | R-MMU-389397, Orexin and neuropeptides FF and QRFP bind to their respective receptors R-MMU-416476, G alpha (q) signalling events |
Names & Taxonomyi
Protein namesi | Recommended name: Orexin receptor type 1Short name: Ox-1-R Short name: Ox1-R Short name: Ox1R Alternative name(s): Hypocretin receptor type 1 |
Gene namesi | Name:Hcrtr1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2385650, Hcrtr1 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Multi-pass membrane protein By similarity
Plasma Membrane
- integral component of plasma membrane Source: UniProtKB
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 46 | ExtracellularBy similarityAdd BLAST | 46 | |
Transmembranei | 47 – 67 | Helical; Name=1By similarityAdd BLAST | 21 | |
Topological domaini | 68 – 82 | CytoplasmicBy similarityAdd BLAST | 15 | |
Transmembranei | 83 – 105 | Helical; Name=2By similarityAdd BLAST | 23 | |
Topological domaini | 106 – 119 | ExtracellularBy similarityAdd BLAST | 14 | |
Transmembranei | 120 – 140 | Helical; Name=3Sequence analysisAdd BLAST | 21 | |
Topological domaini | 141 – 160 | CytoplasmicBy similarityAdd BLAST | 20 | |
Transmembranei | 161 – 182 | Helical; Name=4By similarityAdd BLAST | 22 | |
Topological domaini | 183 – 213 | ExtracellularBy similarityAdd BLAST | 31 | |
Transmembranei | 214 – 235 | Helical; Name=5By similarityAdd BLAST | 22 | |
Topological domaini | 236 – 298 | CytoplasmicBy similarityAdd BLAST | 63 | |
Transmembranei | 299 – 321 | Helical; Name=6By similarityAdd BLAST | 23 | |
Topological domaini | 322 – 336 | ExtracellularBy similarityAdd BLAST | 15 | |
Transmembranei | 337 – 360 | Helical; Name=7By similarityAdd BLAST | 24 | |
Topological domaini | 361 – 416 | CytoplasmicBy similarityAdd BLAST | 56 |
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000069985 | 1 – 416 | Orexin receptor type 1Add BLAST | 416 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 119 ↔ 202 | By similarity | ||
Glycosylationi | 194 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
PaxDbi | P58307 |
PRIDEi | P58307 |
PTM databases
GlyGeni | P58307, 1 site |
iPTMneti | P58307 |
PhosphoSitePlusi | P58307 |
Expressioni
Tissue specificityi
Widely expressed.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000028778, Expressed in substantia nigra and 60 other tissues |
Genevisiblei | P58307, MM |
Interactioni
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000112630 |
Chemistry databases
BindingDBi | P58307 |
Miscellaneous databases
RNActi | P58307, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 26 – 41 | Required for response to orexin-ABy similarityAdd BLAST | 16 |
Domaini
The N-terminal region is required for orexin signaling.By similarity
Sequence similaritiesi
Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3656, Eukaryota |
GeneTreei | ENSGT01010000222375 |
HOGENOMi | CLU_009579_6_3_1 |
InParanoidi | P58307 |
OMAi | LMEPSAT |
OrthoDBi | 981089at2759 |
TreeFami | TF315303 |
Family and domain databases
InterProi | View protein in InterPro IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM IPR000204, Orexin_rcpt IPR004059, Orexin_rcpt_1 |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR00237, GPCRRHODOPSN PR01521, OREXIN1R PR01064, OREXINR |
SMARTi | View protein in SMART SM01381, 7TM_GPCR_Srsx, 1 hit |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P58307-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEPSATPGAQ PGVPTSSGEP FHLPPDYEDE FLRYLWRDYL YPKQYEWVLI
60 70 80 90 100
AAYVAVFLIA LVGNTLVCLA VWRNHHMRTV TNYFIVNLSL ADVLVTAICL
110 120 130 140 150
PASLLVDITE SWLFGQALCK VIPYLQAVSV SVAVLTLSFI ALDRWYAICH
160 170 180 190 200
PLLFKSTARR ARGSILGIWA VSLAVMVPQA AVMECSSVLP ELANRTRLFS
210 220 230 240 250
VCDEHWADEL YPKIYHSCFF IVTYLAPLGL MAMAYFQIFR KLWGRQIPGT
260 270 280 290 300
TSALVRNWKR PSEQLEAQHQ GLCTEPQPRA RAFLAEVKQM RARRKTAKML
310 320 330 340 350
MVVLLVFALC YLPISVLNVL KRVFGMFRQA SDREAVYACF TFSHWLVYAN
360 370 380 390 400
SAANPIIYNF LSGKFREQFK AAFSCCLPGL GPGSSARHKS LSLQSRCSVS
410
KVSEHVVLTT VTTVLS
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 139 | F → V in AAK71326 (Ref. 5) Curated | 1 | |
Sequence conflicti | 232 | A → G in AAR01326 (PubMed:15256537).Curated | 1 | |
Sequence conflicti | 232 | A → G in AAK71326 (Ref. 5) Curated | 1 | |
Sequence conflicti | 232 | A → G in AAO85111 (PubMed:12679517).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY336083 mRNA Translation: AAR01326.1 AK140137 mRNA Translation: BAE24252.1 AL606925 Genomic DNA No translation available. CU207372 Genomic DNA No translation available. CU210846 Genomic DNA No translation available. BC119583 mRNA Translation: AAI19584.1 AF394596 mRNA Translation: AAK71326.1 AY255599 mRNA Translation: AAO85111.1 |
CCDSi | CCDS18707.1 |
RefSeqi | NP_001156499.1, NM_001163027.1 NP_945197.2, NM_198959.2 XP_017175645.1, XM_017320156.1 XP_017175646.1, XM_017320157.1 |
Genome annotation databases
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY336083 mRNA Translation: AAR01326.1 AK140137 mRNA Translation: BAE24252.1 AL606925 Genomic DNA No translation available. CU207372 Genomic DNA No translation available. CU210846 Genomic DNA No translation available. BC119583 mRNA Translation: AAI19584.1 AF394596 mRNA Translation: AAK71326.1 AY255599 mRNA Translation: AAO85111.1 |
CCDSi | CCDS18707.1 |
RefSeqi | NP_001156499.1, NM_001163027.1 NP_945197.2, NM_198959.2 XP_017175645.1, XM_017320156.1 XP_017175646.1, XM_017320157.1 |
3D structure databases
SMRi | P58307 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000112630 |
Chemistry databases
BindingDBi | P58307 |
ChEMBLi | CHEMBL2434819 |
DrugCentrali | P58307 |
Protein family/group databases
GPCRDBi | Search... |
PTM databases
GlyGeni | P58307, 1 site |
iPTMneti | P58307 |
PhosphoSitePlusi | P58307 |
Proteomic databases
PaxDbi | P58307 |
PRIDEi | P58307 |
Protocols and materials databases
Antibodypediai | 2934, 402 antibodies |
Genome annotation databases
Organism-specific databases
CTDi | 3061 |
MGIi | MGI:2385650, Hcrtr1 |
Phylogenomic databases
eggNOGi | KOG3656, Eukaryota |
GeneTreei | ENSGT01010000222375 |
HOGENOMi | CLU_009579_6_3_1 |
InParanoidi | P58307 |
OMAi | LMEPSAT |
OrthoDBi | 981089at2759 |
TreeFami | TF315303 |
Enzyme and pathway databases
Reactomei | R-MMU-389397, Orexin and neuropeptides FF and QRFP bind to their respective receptors R-MMU-416476, G alpha (q) signalling events |
Miscellaneous databases
BioGRID-ORCSi | 230777, 0 hits in 17 CRISPR screens |
ChiTaRSi | Hcrtr1, mouse |
PROi | PR:P58307 |
RNActi | P58307, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000028778, Expressed in substantia nigra and 60 other tissues |
Genevisiblei | P58307, MM |
Family and domain databases
InterProi | View protein in InterPro IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM IPR000204, Orexin_rcpt IPR004059, Orexin_rcpt_1 |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR00237, GPCRRHODOPSN PR01521, OREXIN1R PR01064, OREXINR |
SMARTi | View protein in SMART SM01381, 7TM_GPCR_Srsx, 1 hit |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | OX1R_MOUSE | |
Accessioni | P58307Primary (citable) accession number: P58307 Secondary accession number(s): Q0VDP5 Q80T45 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 26, 2001 |
Last sequence update: | July 27, 2011 | |
Last modified: | February 10, 2021 | |
This is version 151 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- 7-transmembrane G-linked receptors
List of 7-transmembrane G-linked receptor entries - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families