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Protein

Sodium- and chloride-dependent glycine transporter 2

Gene

Slc6a5

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Terminates the action of glycine by its high affinity sodium-dependent reuptake into presynaptic terminals. May be responsible for the termination of neurotransmission at strychnine-sensitive glycinergic synapses.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi208Sodium 1; via carbonyl oxygenBy similarity1
Metal bindingi210Sodium 2; via carbonyl oxygenBy similarity1
Metal bindingi211Sodium 1; via carbonyl oxygenBy similarity1
Metal bindingi215Sodium 2By similarity1
Metal bindingi479Sodium 2By similarity1
Metal bindingi511Sodium 2By similarity1
Metal bindingi576Sodium 1; via carbonyl oxygenBy similarity1
Metal bindingi579Sodium 1By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processNeurotransmitter transport, Symport, Transport
LigandMetal-binding, Sodium

Enzyme and pathway databases

ReactomeiR-RNO-442660 Na+/Cl- dependent neurotransmitter transporters

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium- and chloride-dependent glycine transporter 2
Short name:
GlyT-2
Short name:
GlyT2
Alternative name(s):
Solute carrier family 6 member 5
Gene namesi
Name:Slc6a5
Synonyms:Glyt2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi621824 Slc6a5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 201CytoplasmicSequence analysisAdd BLAST201
Transmembranei202 – 222Helical; Name=1Sequence analysisAdd BLAST21
Transmembranei230 – 249Helical; Name=2Sequence analysisAdd BLAST20
Transmembranei273 – 293Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini294 – 395ExtracellularSequence analysisAdd BLAST102
Transmembranei396 – 414Helical; Name=4Sequence analysisAdd BLAST19
Transmembranei423 – 440Helical; Name=5Sequence analysisAdd BLAST18
Transmembranei476 – 493Helical; Name=6Sequence analysisAdd BLAST18
Transmembranei505 – 526Helical; Name=7Sequence analysisAdd BLAST22
Transmembranei559 – 578Helical; Name=8Sequence analysisAdd BLAST20
Transmembranei606 – 624Helical; Name=9Sequence analysisAdd BLAST19
Transmembranei640 – 660Helical; Name=10Sequence analysisAdd BLAST21
Transmembranei681 – 700Helical; Name=11Sequence analysisAdd BLAST20
Transmembranei719 – 737Helical; Name=12Sequence analysisAdd BLAST19
Topological domaini738 – 799CytoplasmicSequence analysisAdd BLAST62

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3195

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002147641 – 799Sodium- and chloride-dependent glycine transporter 2Add BLAST799

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei58PhosphoserineCombined sources1
Modified residuei59PhosphothreonineBy similarity1
Modified residuei86PhosphoserineBy similarity1
Disulfide bondi313 ↔ 322By similarity
Glycosylationi345N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi355N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi360N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi366N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP58295
PRIDEiP58295

PTM databases

iPTMnetiP58295
PhosphoSitePlusiP58295

Expressioni

Tissue specificityi

Specifically expressed in spinal cord, brain stem, and to a lesser extent in the cerebellum.

Gene expression databases

BgeeiENSRNOG00000031662

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000043665

Chemistry databases

BindingDBiP58295

Structurei

3D structure databases

ProteinModelPortaliP58295
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3660 Eukaryota
COG0733 LUCA
GeneTreeiENSGT00760000118857
HOGENOMiHOG000116406
HOVERGENiHBG071421
InParanoidiP58295
KOiK05038
OMAiVIWIPIM
OrthoDBiEOG091G08PX

Family and domain databases

InterProiView protein in InterPro
IPR000175 Na/ntran_symport
IPR037272 SNS_sf
PANTHERiPTHR11616 PTHR11616, 1 hit
PfamiView protein in Pfam
PF00209 SNF, 1 hit
PRINTSiPR00176 NANEUSMPORT
SUPFAMiSSF161070 SSF161070, 2 hits
PROSITEiView protein in PROSITE
PS00610 NA_NEUROTRAN_SYMP_1, 1 hit
PS00754 NA_NEUROTRAN_SYMP_2, 1 hit
PS50267 NA_NEUROTRAN_SYMP_3, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform a (identifier: P58295-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDCSAPKEMN KPPTNILEAT VPGHRDSPRA PRTSPEQDLP AAAPAAAVQP
60 70 80 90 100
PRVPRSASTG AQTFQSADAR ACEAQRPGVG FCKLSSPQAQ ATSAALRDLS
110 120 130 140 150
EGHSAQANPP SGAAGAGNAL HCKIPALRGP EEDENVSVGK GTLEHNNTPA
160 170 180 190 200
VGWVNMSQST VVLGTDGIAS VLPGSVATTT IPEDEQGDEN KARGNWSSKL
210 220 230 240 250
DFILSMVGYA VGLGNVWRFP YLAFQNGGGA FLIPYLMMLA LAGLPIFFLE
260 270 280 290 300
VSLGQFASQG PVSVWKAIPA LQGCGIAMLI ISVLIAIYYN VIICYTLFYL
310 320 330 340 350
FASFVSVLPW GSCNNPWNTP ECKDKTKLLL DSCVIGDHPK IQIKNSTFCM
360 370 380 390 400
TAYPNLTMVN FTSQANKTFV SGSEEYFKYF VLKISAGIEY PGEIRWPLAF
410 420 430 440 450
CLFLAWVIVY ASLAKGIKTS GKVVYFTATF PYVVLVILLI RGVTLPGAGA
460 470 480 490 500
GIWYFITPKW EKLTDATVWK DAATQIFFSL SAAWGGLITL SSYNKFHNNC
510 520 530 540 550
YRDTLIVTCT NSATSIFAGF VIFSVIGFMA NERKVNIENV ADQGPGIAFV
560 570 580 590 600
VYPEALTRLP LSPFWAIIFF LMLLTLGLDT MFATIETIVT SISDEFPKYL
610 620 630 640 650
RTHKPVFTLG CCICFFIMGF PMITQGGIYM FQLVDTYAAS YALVIIAIFE
660 670 680 690 700
LVGISYVYGL QRFCEDIEMM IGFQPNIFWK VCWAFVTPTI LTFILCFSFY
710 720 730 740 750
QWEPMTYGSY RYPNWSMVLG WLMLACSVIW IPIMFVIKMY LAPGRFIERL
760 770 780 790
KLVCSPQPDW GPFLAQHRGE RYKNMIDPLG TSSLGLKLPV KDLELGTQC
Length:799
Mass (Da):87,842
Last modified:January 20, 2016 - v2
Checksum:i5F0D87B3D2DBE662
GO
Isoform b (identifier: P58295-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-8: Missing.

Show »
Length:791
Mass (Da):86,980
Checksum:i92D1A1C302EF3726
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti139G → A in AAS19315 (PubMed:8226790).Curated1
Sequence conflicti139G → A in AAS49497 (Ref. 2) Curated1
Sequence conflicti150A → P in AAS19315 (PubMed:8226790).Curated1
Sequence conflicti150A → P in AAS49497 (Ref. 2) Curated1
Sequence conflicti399A → P in AAS19315 (PubMed:8226790).Curated1
Sequence conflicti399A → P in AAS49497 (Ref. 2) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0116101 – 8Missing in isoform b. 1 Publication8

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L21672 mRNA Translation: AAS19315.1
AY547309 mRNA Translation: AAS49497.1
AC141159 Genomic DNA No translation available.
PIRiA48716
RefSeqiNP_976079.1, NM_203334.1
XP_017444218.1, XM_017588729.1 [P58295-2]
UniGeneiRn.34566

Genome annotation databases

EnsembliENSRNOT00000041950; ENSRNOP00000043665; ENSRNOG00000031662 [P58295-1]
ENSRNOT00000089693; ENSRNOP00000074102; ENSRNOG00000031662 [P58295-2]
GeneIDi171148
KEGGirno:171148
UCSCiRGD:621824 rat [P58295-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiSC6A5_RAT
AccessioniPrimary (citable) accession number: P58295
Secondary accession number(s): F1LNM6, Q6QDB3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: January 20, 2016
Last modified: May 23, 2018
This is version 114 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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