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UniProtKB - P58295 (SC6A5_RAT)
Protein
Sodium- and chloride-dependent glycine transporter 2
Gene
Slc6a5
Organism
Rattus norvegicus (Rat)
Status
Functioni
Sodium- and chloride-dependent glycine transporter (PubMed:8226790).
Terminates the action of glycine by its high affinity sodium-dependent reuptake into presynaptic terminals (PubMed:8226790).
May be responsible for the termination of neurotransmission at strychnine-sensitive glycinergic synapses (PubMed:8226790).
1 PublicationSites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 208 | Sodium 1; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 210 | Sodium 2; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 211 | Sodium 1; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 215 | Sodium 2By similarity | 1 | |
Metal bindingi | 479 | Sodium 2By similarity | 1 | |
Metal bindingi | 511 | Sodium 2By similarity | 1 | |
Metal bindingi | 576 | Sodium 1; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 579 | Sodium 1By similarity | 1 |
GO - Molecular functioni
- glycine:sodium symporter activity Source: RGD
- glycine transmembrane transporter activity Source: RGD
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- glycine import across plasma membrane Source: RGD
- glycine transport Source: RGD
- neurotransmitter reuptake Source: RGD
- neurotransmitter uptake Source: SynGO
- sodium ion transmembrane transport Source: GO_Central
- synaptic transmission, glycinergic Source: RGD
Keywordsi
Biological process | Neurotransmitter transport, Symport, Transport |
Ligand | Metal-binding, Sodium |
Enzyme and pathway databases
Reactomei | R-RNO-442660, Na+/Cl- dependent neurotransmitter transporters |
Names & Taxonomyi
Protein namesi | Recommended name: Sodium- and chloride-dependent glycine transporter 2Short name: GlyT-2 Short name: GlyT2 Alternative name(s): Solute carrier family 6 member 5 |
Gene namesi | Name:Slc6a5 Synonyms:Glyt2 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 621824, Slc6a5 |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication; Multi-pass membrane protein Sequence analysis
Endosome
- endosome Source: ARUK-UCL
Plasma Membrane
- integral component of plasma membrane Source: GO_Central
- integral component of presynaptic membrane Source: SynGO
- plasma membrane Source: RGD
Other locations
- dense core granule Source: ARUK-UCL
- glycinergic synapse Source: SynGO
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 201 | CytoplasmicSequence analysisAdd BLAST | 201 | |
Transmembranei | 202 – 222 | Helical; Name=1Sequence analysisAdd BLAST | 21 | |
Transmembranei | 230 – 249 | Helical; Name=2Sequence analysisAdd BLAST | 20 | |
Transmembranei | 273 – 293 | Helical; Name=3Sequence analysisAdd BLAST | 21 | |
Topological domaini | 294 – 395 | ExtracellularSequence analysisAdd BLAST | 102 | |
Transmembranei | 396 – 414 | Helical; Name=4Sequence analysisAdd BLAST | 19 | |
Transmembranei | 423 – 440 | Helical; Name=5Sequence analysisAdd BLAST | 18 | |
Transmembranei | 476 – 493 | Helical; Name=6Sequence analysisAdd BLAST | 18 | |
Transmembranei | 505 – 526 | Helical; Name=7Sequence analysisAdd BLAST | 22 | |
Transmembranei | 559 – 578 | Helical; Name=8Sequence analysisAdd BLAST | 20 | |
Transmembranei | 606 – 624 | Helical; Name=9Sequence analysisAdd BLAST | 19 | |
Transmembranei | 640 – 660 | Helical; Name=10Sequence analysisAdd BLAST | 21 | |
Transmembranei | 681 – 700 | Helical; Name=11Sequence analysisAdd BLAST | 20 | |
Transmembranei | 719 – 737 | Helical; Name=12Sequence analysisAdd BLAST | 19 | |
Topological domaini | 738 – 799 | CytoplasmicSequence analysisAdd BLAST | 62 |
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000214764 | 1 – 799 | Sodium- and chloride-dependent glycine transporter 2Add BLAST | 799 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 58 | PhosphoserineCombined sources | 1 | |
Modified residuei | 59 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 86 | PhosphoserineBy similarity | 1 | |
Disulfide bondi | 313 ↔ 322 | By similarity | ||
Glycosylationi | 345 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 355 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 360 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 366 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Post-translational modificationi
N-glycosylated.By similarity
Keywords - PTMi
Disulfide bond, Glycoprotein, PhosphoproteinProteomic databases
PaxDbi | P58295 |
PTM databases
GlyGeni | P58295, 4 sites |
iPTMneti | P58295 |
PhosphoSitePlusi | P58295 |
Expressioni
Tissue specificityi
Specifically expressed in spinal cord, brain stem, and to a lesser extent in the cerebellum.
Gene expression databases
Bgeei | ENSRNOG00000031662, Expressed in cerebellum and 8 other tissues |
Interactioni
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000043665 |
Chemistry databases
BindingDBi | P58295 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 64 | DisorderedSequence analysisAdd BLAST | 64 |
Sequence similaritiesi
Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A5 subfamily. [View classification]Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3660, Eukaryota |
GeneTreei | ENSGT00940000154963 |
InParanoidi | P58295 |
OMAi | GAPKEMN |
OrthoDBi | 250396at2759 |
TreeFami | TF343812 |
Family and domain databases
InterProi | View protein in InterPro IPR000175, Na/ntran_symport IPR037272, SNS_sf |
PANTHERi | PTHR11616, PTHR11616, 1 hit |
Pfami | View protein in Pfam PF00209, SNF, 1 hit |
PRINTSi | PR00176, NANEUSMPORT |
SUPFAMi | SSF161070, SSF161070, 1 hit |
PROSITEi | View protein in PROSITE PS00610, NA_NEUROTRAN_SYMP_1, 1 hit PS00754, NA_NEUROTRAN_SYMP_2, 1 hit PS50267, NA_NEUROTRAN_SYMP_3, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketIsoform a (identifier: P58295-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MDCSAPKEMN KPPTNILEAT VPGHRDSPRA PRTSPEQDLP AAAPAAAVQP
60 70 80 90 100
PRVPRSASTG AQTFQSADAR ACEAQRPGVG FCKLSSPQAQ ATSAALRDLS
110 120 130 140 150
EGHSAQANPP SGAAGAGNAL HCKIPALRGP EEDENVSVGK GTLEHNNTPA
160 170 180 190 200
VGWVNMSQST VVLGTDGIAS VLPGSVATTT IPEDEQGDEN KARGNWSSKL
210 220 230 240 250
DFILSMVGYA VGLGNVWRFP YLAFQNGGGA FLIPYLMMLA LAGLPIFFLE
260 270 280 290 300
VSLGQFASQG PVSVWKAIPA LQGCGIAMLI ISVLIAIYYN VIICYTLFYL
310 320 330 340 350
FASFVSVLPW GSCNNPWNTP ECKDKTKLLL DSCVIGDHPK IQIKNSTFCM
360 370 380 390 400
TAYPNLTMVN FTSQANKTFV SGSEEYFKYF VLKISAGIEY PGEIRWPLAF
410 420 430 440 450
CLFLAWVIVY ASLAKGIKTS GKVVYFTATF PYVVLVILLI RGVTLPGAGA
460 470 480 490 500
GIWYFITPKW EKLTDATVWK DAATQIFFSL SAAWGGLITL SSYNKFHNNC
510 520 530 540 550
YRDTLIVTCT NSATSIFAGF VIFSVIGFMA NERKVNIENV ADQGPGIAFV
560 570 580 590 600
VYPEALTRLP LSPFWAIIFF LMLLTLGLDT MFATIETIVT SISDEFPKYL
610 620 630 640 650
RTHKPVFTLG CCICFFIMGF PMITQGGIYM FQLVDTYAAS YALVIIAIFE
660 670 680 690 700
LVGISYVYGL QRFCEDIEMM IGFQPNIFWK VCWAFVTPTI LTFILCFSFY
710 720 730 740 750
QWEPMTYGSY RYPNWSMVLG WLMLACSVIW IPIMFVIKMY LAPGRFIERL
760 770 780 790
KLVCSPQPDW GPFLAQHRGE RYKNMIDPLG TSSLGLKLPV KDLELGTQC
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 139 | G → A in AAS19315 (PubMed:8226790).Curated | 1 | |
Sequence conflicti | 139 | G → A in AAS49497 (Ref. 2) Curated | 1 | |
Sequence conflicti | 150 | A → P in AAS19315 (PubMed:8226790).Curated | 1 | |
Sequence conflicti | 150 | A → P in AAS49497 (Ref. 2) Curated | 1 | |
Sequence conflicti | 399 | A → P in AAS19315 (PubMed:8226790).Curated | 1 | |
Sequence conflicti | 399 | A → P in AAS49497 (Ref. 2) Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_011610 | 1 – 8 | Missing in isoform b. 1 Publication | 8 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L21672 mRNA Translation: AAS19315.1 AY547309 mRNA Translation: AAS49497.1 AC141159 Genomic DNA No translation available. |
PIRi | A48716 |
RefSeqi | NP_976079.1, NM_203334.1 XP_017444218.1, XM_017588729.1 [P58295-2] |
Genome annotation databases
Ensembli | ENSRNOT00000041950; ENSRNOP00000043665; ENSRNOG00000031662 [P58295-1] |
GeneIDi | 171148 |
KEGGi | rno:171148 |
UCSCi | RGD:621824, rat [P58295-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L21672 mRNA Translation: AAS19315.1 AY547309 mRNA Translation: AAS49497.1 AC141159 Genomic DNA No translation available. |
PIRi | A48716 |
RefSeqi | NP_976079.1, NM_203334.1 XP_017444218.1, XM_017588729.1 [P58295-2] |
3D structure databases
AlphaFoldDBi | P58295 |
SMRi | P58295 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000043665 |
Chemistry databases
BindingDBi | P58295 |
ChEMBLi | CHEMBL3195 |
PTM databases
GlyGeni | P58295, 4 sites |
iPTMneti | P58295 |
PhosphoSitePlusi | P58295 |
Proteomic databases
PaxDbi | P58295 |
Genome annotation databases
Ensembli | ENSRNOT00000041950; ENSRNOP00000043665; ENSRNOG00000031662 [P58295-1] |
GeneIDi | 171148 |
KEGGi | rno:171148 |
UCSCi | RGD:621824, rat [P58295-1] |
Organism-specific databases
CTDi | 9152 |
RGDi | 621824, Slc6a5 |
Phylogenomic databases
eggNOGi | KOG3660, Eukaryota |
GeneTreei | ENSGT00940000154963 |
InParanoidi | P58295 |
OMAi | GAPKEMN |
OrthoDBi | 250396at2759 |
TreeFami | TF343812 |
Enzyme and pathway databases
Reactomei | R-RNO-442660, Na+/Cl- dependent neurotransmitter transporters |
Miscellaneous databases
PROi | PR:P58295 |
Gene expression databases
Bgeei | ENSRNOG00000031662, Expressed in cerebellum and 8 other tissues |
Family and domain databases
InterProi | View protein in InterPro IPR000175, Na/ntran_symport IPR037272, SNS_sf |
PANTHERi | PTHR11616, PTHR11616, 1 hit |
Pfami | View protein in Pfam PF00209, SNF, 1 hit |
PRINTSi | PR00176, NANEUSMPORT |
SUPFAMi | SSF161070, SSF161070, 1 hit |
PROSITEi | View protein in PROSITE PS00610, NA_NEUROTRAN_SYMP_1, 1 hit PS00754, NA_NEUROTRAN_SYMP_2, 1 hit PS50267, NA_NEUROTRAN_SYMP_3, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | SC6A5_RAT | |
Accessioni | P58295Primary (citable) accession number: P58295 Secondary accession number(s): F1LNM6, Q6QDB3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 26, 2001 |
Last sequence update: | January 20, 2016 | |
Last modified: | May 25, 2022 | |
This is version 134 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families