UniProtKB - P58281 (OPA1_MOUSE)
Dynamin-like 120 kDa protein, mitochondrial
Opa1
Functioni
Miscellaneous
Catalytic activityi
- EC:3.6.5.5By similarity
Activity regulationi
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 295 – 302 | GTPSequence analysis | 8 | |
Nucleotide bindingi | 398 – 402 | GTPSequence analysis | 5 | |
Nucleotide bindingi | 467 – 470 | GTPSequence analysis | 4 |
GO - Molecular functioni
- cardiolipin binding Source: UniProtKB
- GTPase activity Source: UniProtKB
- GTP binding Source: UniProtKB-KW
- kinase binding Source: MGI
- microtubule binding Source: GO_Central
- phosphatidic acid binding Source: UniProtKB
- protein-containing complex binding Source: MGI
GO - Biological processi
- apoptotic process Source: UniProtKB-KW
- calcium import into the mitochondrion Source: MGI
- cellular senescence Source: MGI
- dynamin family protein polymerization involved in mitochondrial fission Source: GO_Central
- GTP metabolic process Source: UniProtKB
- inner mitochondrial membrane organization Source: UniProtKB
- intracellular distribution of mitochondria Source: MGI
- membrane fusion Source: GO_Central
- membrane tubulation Source: UniProtKB
- mitochondrial fission Source: GO_Central
- mitochondrial fusion Source: MGI
- mitochondrial genome maintenance Source: UniProtKB
- mitochondrion morphogenesis Source: MGI
- mitochondrion organization Source: MGI
- negative regulation of apoptotic process Source: UniProtKB
- negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway Source: ParkinsonsUK-UCL
- negative regulation of intrinsic apoptotic signaling pathway Source: UniProtKB
- negative regulation of release of cytochrome c from mitochondria Source: UniProtKB
- neural tube closure Source: MGI
- organelle fission Source: GO_Central
- positive regulation of cellular response to insulin stimulus Source: MGI
- positive regulation of dendrite development Source: MGI
- positive regulation of dendritic spine morphogenesis Source: MGI
- positive regulation of insulin receptor signaling pathway Source: MGI
- positive regulation of mitochondrial fusion Source: MGI
- positive regulation of neuron maturation Source: MGI
- protein complex oligomerization Source: MGI
- visual perception Source: UniProtKB
Keywordsi
Molecular function | Hydrolase |
Biological process | Apoptosis, Sensory transduction, Vision |
Ligand | GTP-binding, Lipid-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 3.6.5.5, 3474 |
Reactomei | R-MMU-169911, Regulation of Apoptosis |
Names & Taxonomyi
Protein namesi | Recommended name: Dynamin-like 120 kDa protein, mitochondrial (EC:3.6.5.5)Alternative name(s): Large GTP-binding protein Short name: LargeG Optic atrophy protein 1 homolog Cleaved into the following chain: |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1921393, Opa1 |
Subcellular locationi
Mitochondrion
- Mitochondrion inner membrane 3 Publications; Single-pass membrane protein Sequence analysis
- Mitochondrion 1 Publication
- Mitochondrion membrane By similarity
Note: Detected at contact sites between endoplasmic reticulum and mitochondrion membranes.By similarity
Cytosol
- cytosol Source: MGI
Mitochondrion
- extrinsic component of mitochondrial inner membrane Source: MGI
- mitochondrial crista Source: UniProtKB
- mitochondrial inner membrane Source: UniProtKB
- mitochondrial intermembrane space Source: UniProtKB
- mitochondrial membrane Source: GO_Central
- mitochondrial outer membrane Source: UniProtKB
- mitochondrion Source: MGI
Nucleus
- nucleoplasm Source: MGI
Other locations
- cytoplasm Source: GO_Central
- dendrite Source: UniProtKB
- integral component of membrane Source: UniProtKB-KW
- membrane Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 88 – 96 | Mitochondrial matrixSequence analysis | 9 | |
Transmembranei | 97 – 113 | HelicalSequence analysisAdd BLAST | 17 | |
Topological domaini | 114 – 960 | Mitochondrial intermembraneSequence analysisAdd BLAST | 847 |
Keywords - Cellular componenti
Membrane, Mitochondrion, Mitochondrion inner membranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 87 | MitochondrionBy similarityAdd BLAST | 87 | |
ChainiPRO_0000007398 | 88 – 960 | Dynamin-like 120 kDa protein, mitochondrialAdd BLAST | 873 | |
ChainiPRO_0000253480 | 195 – 960 | Dynamin-like 120 kDa protein, form S1By similarityAdd BLAST | 766 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 228 | N6-acetyllysineBy similarity | 1 |
Post-translational modificationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 194 – 195 | Cleavage at site S1By similarity | 2 |
Keywords - PTMi
AcetylationProteomic databases
jPOSTi | P58281 |
MaxQBi | P58281 |
PaxDbi | P58281 |
PeptideAtlasi | P58281 |
PRIDEi | P58281 |
PTM databases
iPTMneti | P58281 |
PhosphoSitePlusi | P58281 |
SwissPalmi | P58281 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000038084, Expressed in brain and 279 other tissues |
ExpressionAtlasi | P58281, baseline and differential |
Genevisiblei | P58281, MM |
Interactioni
Subunit structurei
Oligomeric complex consisting of membrane-bound and soluble forms of OPA1 (PubMed:16839885).
Interacts with CHCHD3 and IMMT; these interactions occur preferentially with soluble OPA1 forms (PubMed:21081504).
Interacts with RCC1L; this interaction is direct (PubMed:28746876). Binds PARL (PubMed:16839884).
Interacts with PRELID1 (By similarity).
By similarity4 PublicationsGO - Molecular functioni
- kinase binding Source: MGI
- microtubule binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 216522, 9 interactors |
IntActi | P58281, 18 interactors |
MINTi | P58281 |
STRINGi | 10090.ENSMUSP00000124223 |
Miscellaneous databases
RNActi | P58281, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 285 – 561 | Dynamin-type GPROSITE-ProRule annotationAdd BLAST | 277 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 295 – 302 | G1 motifPROSITE-ProRule annotation | 8 | |
Regioni | 321 – 324 | G2 motifPROSITE-ProRule annotation | 4 | |
Regioni | 398 – 401 | G3 motifPROSITE-ProRule annotation | 4 | |
Regioni | 467 – 470 | G4 motifPROSITE-ProRule annotation | 4 | |
Regioni | 501 – 504 | G5 motifPROSITE-ProRule annotation | 4 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 210 – 254 | Sequence analysisAdd BLAST | 45 | |
Coiled coili | 895 – 960 | Sequence analysisAdd BLAST | 66 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length | |
---|---|---|---|---|---|
Isoform 2 (identifier: P58281-2) | |||||
Motifi | 217 – 222 | LQQQIQ motifBy similarity | 6 |
Sequence similaritiesi
Keywords - Domaini
Coiled coil, Transit peptide, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0447, Eukaryota |
GeneTreei | ENSGT00550000074851 |
HOGENOMi | CLU_012302_0_0_1 |
InParanoidi | P58281 |
OMAi | YEDWKDG |
OrthoDBi | 609213at2759 |
PhylomeDBi | P58281 |
TreeFami | TF314250 |
Family and domain databases
CDDi | cd08771, DLP_1, 1 hit |
InterProi | View protein in InterPro IPR001401, Dynamin_GTPase IPR022812, Dynamin_SF IPR030381, G_DYNAMIN_dom IPR033047, Opa1 IPR027417, P-loop_NTPase |
PANTHERi | PTHR11566, PTHR11566, 1 hit PTHR11566:SF67, PTHR11566:SF67, 1 hit |
Pfami | View protein in Pfam PF00350, Dynamin_N, 1 hit |
PRINTSi | PR00195, DYNAMIN |
SMARTi | View protein in SMART SM00053, DYNc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS51718, G_DYNAMIN_2, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MWRAGRAAVA CEVCQSLVKH SSGIQRNVPL QKLHLVSRSI YRSHHPALKL
60 70 80 90 100
QRPQLRTPFQ QFSSLTHLSL HKLKLSPIKY GYQPRRNFWP ARLAARLLKL
110 120 130 140 150
RYIILGSAVG GGYTAKKTFD EWKDMIPDLS DYKWIVPDFI WEIDEYIDLE
160 170 180 190 200
KIRKALPSSE DLASLAPDLD KITESLSLLK DFFTAGSPGE TAFRATDHGS
210 220 230 240 250
ESDKHYRKVS DKEKIDQLQE ELLHTQLKYQ RILERLEKEN KELRKLVLQK
260 270 280 290 300
DDKGIHHRKL KKSLIDMYSE VLDVLSDYDA SYNTQDHLPR VVVVGDQSAG
310 320 330 340 350
KTSVLEMIAQ ARIFPRGSGE MMTRSPVKVT LSEGPHHVAL FKDSSREFDL
360 370 380 390 400
TKEEDLAALR HEIELRMRKN VKEGCTVSPE TISLNVKGPG LQRMVLVDLP
410 420 430 440 450
GVINTVTSGM APDTKETIFS ISKAYMQNPN AIILCIQDGS VDAERSIVTD
460 470 480 490 500
LVSQMDPHGR RTIFVLTKVD LAEKNVASPS RIQQIIEGKL FPMKALGYFA
510 520 530 540 550
VVTGKGNSSE SIEAIREYEE EFFQNSKLLK TSMLKAHQVT TRNLSLAVSD
560 570 580 590 600
CFWKMVRESV EQQADSFKAT RFNLETEWKN NYPRLRELDR NELFEKAKNE
610 620 630 640 650
ILDEVISLSQ VTPKHWEEIL QQSLWERVST HVIENIYLPA AQTMNSGTFN
660 670 680 690 700
TTVDIKLKQW TDKQLPNKAV EVAWETLQEE FSRFMTEPKG KEHDDIFDKL
710 720 730 740 750
KEAVKEESIK RHKWNDFAED SLRVIQHNAL EDRSISDKQQ WDAAIYFMEE
760 770 780 790 800
ALQGRLKDTE NAIENMIGPD WKKRWMYWKN RTQEQCVHNE TKNELEKMLK
810 820 830 840 850
VNDEHPAYLA SDEITTVRKN LESRGVEVDP SLIKDTWHQV YRRHFLKTAL
860 870 880 890 900
NHCNLCRRGF YYYQRHFIDS ELECNDVVLF WRIQRMLAIT ANTLRQQLTN
910 920 930 940 950
TEVRRLEKNV KEVLEDFAED GEKKVKLLTG KRVQLAEDLK KVREIQEKLD
960
AFIEALHQEK
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketH7BX01 | H7BX01_MOUSE | Dynamin-like 120 kDa protein, form ... | Opa1 | 978 | Annotation score: | ||
E0CXD1 | E0CXD1_MOUSE | Dynamin-like 120 kDa protein, form ... | Opa1 | 692 | Annotation score: | ||
F6U775 | F6U775_MOUSE | Dynamin GTPase | Opa1 | 296 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 236 | L → P in BAC30002 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 330 – 331 | TL → NN in BAC32021 (PubMed:16141072).Curated | 2 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_021037 | 208 | K → KGLLGELILLQQQIQEHEEE ARRAAGQYSTSYAQQKRK in isoform 2. 1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB044138 mRNA Translation: BAB59000.1 AK029157 mRNA Translation: BAC26331.1 AK038446 mRNA Translation: BAC30002.1 AK044657 mRNA Translation: BAC32021.1 AK050383 mRNA Translation: BAC34224.1 AK145620 mRNA Translation: BAE26544.1 BC025160 mRNA Translation: AAH25160.1 Different initiation. BC138665 mRNA Translation: AAI38666.1 BC145959 mRNA Translation: AAI45960.1 |
CCDSi | CCDS28096.1 [P58281-1] |
RefSeqi | NP_598513.1, NM_133752.3 [P58281-1] XP_006522720.1, XM_006522657.2 |
Genome annotation databases
Ensembli | ENSMUST00000160597; ENSMUSP00000124223; ENSMUSG00000038084 [P58281-1] ENSMUST00000161186; ENSMUSP00000123880; ENSMUSG00000038084 [P58281-2] |
GeneIDi | 74143 |
KEGGi | mmu:74143 |
UCSCi | uc007ywf.2, mouse [P58281-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB044138 mRNA Translation: BAB59000.1 AK029157 mRNA Translation: BAC26331.1 AK038446 mRNA Translation: BAC30002.1 AK044657 mRNA Translation: BAC32021.1 AK050383 mRNA Translation: BAC34224.1 AK145620 mRNA Translation: BAE26544.1 BC025160 mRNA Translation: AAH25160.1 Different initiation. BC138665 mRNA Translation: AAI38666.1 BC145959 mRNA Translation: AAI45960.1 |
CCDSi | CCDS28096.1 [P58281-1] |
RefSeqi | NP_598513.1, NM_133752.3 [P58281-1] XP_006522720.1, XM_006522657.2 |
3D structure databases
SMRi | P58281 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 216522, 9 interactors |
IntActi | P58281, 18 interactors |
MINTi | P58281 |
STRINGi | 10090.ENSMUSP00000124223 |
PTM databases
iPTMneti | P58281 |
PhosphoSitePlusi | P58281 |
SwissPalmi | P58281 |
Proteomic databases
jPOSTi | P58281 |
MaxQBi | P58281 |
PaxDbi | P58281 |
PeptideAtlasi | P58281 |
PRIDEi | P58281 |
Protocols and materials databases
Antibodypediai | 33885, 299 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000160597; ENSMUSP00000124223; ENSMUSG00000038084 [P58281-1] ENSMUST00000161186; ENSMUSP00000123880; ENSMUSG00000038084 [P58281-2] |
GeneIDi | 74143 |
KEGGi | mmu:74143 |
UCSCi | uc007ywf.2, mouse [P58281-1] |
Organism-specific databases
CTDi | 4976 |
MGIi | MGI:1921393, Opa1 |
Phylogenomic databases
eggNOGi | KOG0447, Eukaryota |
GeneTreei | ENSGT00550000074851 |
HOGENOMi | CLU_012302_0_0_1 |
InParanoidi | P58281 |
OMAi | YEDWKDG |
OrthoDBi | 609213at2759 |
PhylomeDBi | P58281 |
TreeFami | TF314250 |
Enzyme and pathway databases
BRENDAi | 3.6.5.5, 3474 |
Reactomei | R-MMU-169911, Regulation of Apoptosis |
Miscellaneous databases
BioGRID-ORCSi | 74143, 7 hits in 17 CRISPR screens |
ChiTaRSi | Opa1, mouse |
PROi | PR:P58281 |
RNActi | P58281, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000038084, Expressed in brain and 279 other tissues |
ExpressionAtlasi | P58281, baseline and differential |
Genevisiblei | P58281, MM |
Family and domain databases
CDDi | cd08771, DLP_1, 1 hit |
InterProi | View protein in InterPro IPR001401, Dynamin_GTPase IPR022812, Dynamin_SF IPR030381, G_DYNAMIN_dom IPR033047, Opa1 IPR027417, P-loop_NTPase |
PANTHERi | PTHR11566, PTHR11566, 1 hit PTHR11566:SF67, PTHR11566:SF67, 1 hit |
Pfami | View protein in Pfam PF00350, Dynamin_N, 1 hit |
PRINTSi | PR00195, DYNAMIN |
SMARTi | View protein in SMART SM00053, DYNc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS51718, G_DYNAMIN_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | OPA1_MOUSE | |
Accessioni | P58281Primary (citable) accession number: P58281 Secondary accession number(s): A6H6Q3 Q8R3J7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 26, 2001 |
Last sequence update: | September 26, 2001 | |
Last modified: | December 2, 2020 | |
This is version 163 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families