UniProtKB - P58252 (EF2_MOUSE)
Protein
Elongation factor 2
Gene
Eef2
Organism
Mus musculus (Mouse)
Status
Functioni
Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 26 – 33 | GTPBy similarity | 8 | |
Nucleotide bindingi | 104 – 108 | GTPBy similarity | 5 | |
Nucleotide bindingi | 158 – 161 | GTPBy similarity | 4 |
GO - Molecular functioni
- 5S rRNA binding Source: MGI
- actin filament binding Source: MGI
- GTPase activity Source: MGI
- GTP binding Source: UniProtKB-KW
- p53 binding Source: MGI
- protein kinase binding Source: MGI
- ribosome binding Source: MGI
- RNA binding Source: MGI
- translation elongation factor activity Source: GO_Central
GO - Biological processi
- aging Source: Ensembl
- cellular response to brain-derived neurotrophic factor stimulus Source: Ensembl
- glial cell proliferation Source: Ensembl
- hematopoietic progenitor cell differentiation Source: MGI
- positive regulation of cytoplasmic translation Source: MGI
- positive regulation of translation Source: MGI
- response to drug Source: Ensembl
- response to endoplasmic reticulum stress Source: Ensembl
- response to estradiol Source: Ensembl
- response to ethanol Source: Ensembl
- response to folic acid Source: Ensembl
- response to hydrogen peroxide Source: Ensembl
- response to ischemia Source: Ensembl
- skeletal muscle cell differentiation Source: Ensembl
- skeletal muscle contraction Source: Ensembl
- translational elongation Source: MGI
Keywordsi
Molecular function | Elongation factor |
Biological process | Protein biosynthesis |
Ligand | GTP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-MMU-156902, Peptide chain elongation R-MMU-5358493, Synthesis of diphthamide-EEF2 R-MMU-6798695, Neutrophil degranulation R-MMU-8876725, Protein methylation |
Names & Taxonomyi
Protein namesi | Recommended name: Elongation factor 2Short name: EF-2 |
Gene namesi | Name:Eef2 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:95288, Eef2 |
Subcellular locationi
Cytosol
- cytosol Source: MGI
Plasma Membrane
- plasma membrane Source: MGI
Other locations
- aggresome Source: MGI
- cytoplasm Source: UniProtKB
- membrane raft Source: MGI
- polysomal ribosome Source: MGI
- polysome Source: MGI
- ribonucleoprotein complex Source: MGI
- synapse Source: SynGO
Keywords - Cellular componenti
Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed2 Publications | |||
ChainiPRO_0000091002 | 2 – 858 | Elongation factor 2Add BLAST | 857 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 54 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 57 | Phosphothreonine; by EEF2KBy similarity | 1 | |
Modified residuei | 59 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 152 | N6-succinyllysineCombined sources | 1 | |
Modified residuei | 235 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 239 | N6-acetyllysine; alternateCombined sources | 1 | |
Cross-linki | 239 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity | ||
Modified residuei | 265 | Phosphotyrosine; by CSKBy similarity | 1 | |
Modified residuei | 272 | N6-acetyllysine; alternateCombined sources | 1 | |
Modified residuei | 272 | N6-succinyllysine; alternateCombined sources | 1 | |
Modified residuei | 275 | N6-acetyllysineCombined sources | 1 | |
Cross-linki | 322 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity | ||
Modified residuei | 325 | PhosphoserineBy similarity | 1 | |
Modified residuei | 373 | Phosphotyrosine; by CSKBy similarity | 1 | |
Modified residuei | 435 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 439 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 445 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 502 | PhosphoserineBy similarity | 1 | |
Modified residuei | 525 | N6,N6,N6-trimethyllysine; by EEF2KMTBy similarity | 1 | |
Cross-linki | 529 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity | ||
Modified residuei | 572 | N6-succinyllysineCombined sources | 1 | |
Modified residuei | 595 | Phosphoserine; by CDK2By similarity | 1 | |
Modified residuei | 619 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 715 | DiphthamideBy similarity | 1 |
Post-translational modificationi
Phosphorylation by EF-2 kinase completely inactivates EF-2; it requires prior phosphorylation by CDK2 at Ser-595 during mitotic prometaphase. Phosphorylation by CSK promotes SUMOylation, proteolytic cleavage, and nuclear translocation if the C-terminal fragment.By similarity
Diphthamide is 2-[3-carboxyamido-3-(trimethyl-ammonio)propyl]histidine (By similarity).By similarity
ISGylated.1 Publication
Proteolytically processed at two sites following phosphorylation by CSK.By similarity
SUMOylated following phosphorylation by CSK, promotes proteolytic cleavage.By similarity
Keywords - PTMi
Acetylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugationProteomic databases
CPTACi | non-CPTAC-3912 |
EPDi | P58252 |
jPOSTi | P58252 |
MaxQBi | P58252 |
PaxDbi | P58252 |
PeptideAtlasi | P58252 |
PRIDEi | P58252 |
2D gel databases
COMPLUYEAST-2DPAGEi | P58252 |
REPRODUCTION-2DPAGEi | P58252 |
PTM databases
iPTMneti | P58252 |
MetOSitei | P58252 |
PhosphoSitePlusi | P58252 |
SwissPalmi | P58252 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000034994, Expressed in male reproductive system and 324 other tissues |
Genevisiblei | P58252, MM |
Interactioni
Subunit structurei
Component of the mRNA surveillance SURF complex, at least composed of ERF1, ERF3 (ERF3A or ERF3B), EEF2, UPF1/RENT1, SMG1, SMG8 and SMG9.
Interacts with RBPMS2.
By similarityGO - Molecular functioni
- actin filament binding Source: MGI
- p53 binding Source: MGI
- protein kinase binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 199387, 88 interactors |
IntActi | P58252, 20 interactors |
MINTi | P58252 |
STRINGi | 10090.ENSMUSP00000046101 |
Miscellaneous databases
RNActi | P58252, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 17 – 362 | tr-type GPROSITE-ProRule annotationAdd BLAST | 346 |
Sequence similaritiesi
Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.PROSITE-ProRule annotation
Phylogenomic databases
eggNOGi | KOG0469, Eukaryota |
GeneTreei | ENSGT00940000154662 |
HOGENOMi | CLU_002794_11_1_1 |
InParanoidi | P58252 |
OMAi | GVMTQTE |
OrthoDBi | 140796at2759 |
PhylomeDBi | P58252 |
TreeFami | TF300575 |
Family and domain databases
Gene3Di | 3.30.230.10, 1 hit |
InterProi | View protein in InterPro IPR041095, EFG_II IPR035647, EFG_III/V IPR000640, EFG_V-like IPR004161, EFTu-like_2 IPR031157, G_TR_CS IPR027417, P-loop_NTPase IPR020568, Ribosomal_S5_D2-typ_fold IPR014721, Ribosomal_S5_D2-typ_fold_subgr IPR005225, Small_GTP-bd_dom IPR000795, T_Tr_GTP-bd_dom IPR009000, Transl_B-barrel_sf IPR005517, Transl_elong_EFG/EF2_IV |
Pfami | View protein in Pfam PF00679, EFG_C, 1 hit PF14492, EFG_III, 1 hit PF03764, EFG_IV, 1 hit PF00009, GTP_EFTU, 1 hit PF03144, GTP_EFTU_D2, 1 hit |
PRINTSi | PR00315, ELONGATNFCT |
SMARTi | View protein in SMART SM00838, EFG_C, 1 hit SM00889, EFG_IV, 1 hit |
SUPFAMi | SSF50447, SSF50447, 1 hit SSF52540, SSF52540, 1 hit SSF54211, SSF54211, 1 hit SSF54980, SSF54980, 2 hits |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS00301, G_TR_1, 1 hit PS51722, G_TR_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P58252-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVNFTVDQIR AIMDKKANIR NMSVIAHVDH GKSTLTDSLV CKAGIIASAR
60 70 80 90 100
AGETRFTDTR KDEQERCITI KSTAISLFYE LSENDLNFIK QSKDGSGFLI
110 120 130 140 150
NLIDSPGHVD FSSEVTAALR VTDGALVVVD CVSGVCVQTE TVLRQAIAER
160 170 180 190 200
IKPVLMMNKM DRALLELQLE PEELYQTFQR IVENVNVIIS TYGEGESGPM
210 220 230 240 250
GNIMIDPVLG TVGFGSGLHG WAFTLKQFAE MYVAKFAAKG EGQLSAAERA
260 270 280 290 300
KKVEDMMKKL WGDRYFDPAN GKFSKSANSP DGKKLPRTFC QLILDPIFKV
310 320 330 340 350
FDAIMNFRKE ETAKLIEKLD IKLDSEDKDK EGKPLLKAVM RRWLPAGDAL
360 370 380 390 400
LQMITIHLPS PVTAQKYRCE LLYEGPPDDE AAMGIKSCDP KGPLMMYISK
410 420 430 440 450
MVPTSDKGRF YAFGRVFSGV VSTGLKVRIM GPNYTPGKKE DLYLKPIQRT
460 470 480 490 500
ILMMGRYVEP IEDVPCGNIV GLVGVDQFLV KTGTITTFEH AHNMRVMKFS
510 520 530 540 550
VSPVVRVAVE AKNPADLPKL VEGLKRLAKS DPMVQCIIEE SGEHIIAGAG
560 570 580 590 600
ELHLEICLKD LEEDHACIPI KKSDPVVSYR ETVSEESNVL CLSKSPNKHN
610 620 630 640 650
RLYMKARPFP DGLAEDIDKG EVSARQELKA RARYLAEKYE WDVAEARKIW
660 670 680 690 700
CFGPDGTGPN ILTDITKGVQ YLNEIKDSVV AGFQWATKEG ALCEENMRGV
710 720 730 740 750
RFDVHDVTLH ADAIHRGGGQ IIPTARRCLY ASVLTAQPRL MEPIYLVEIQ
760 770 780 790 800
CPEQVVGGIY GVLNRKRGHV FEESQVAGTP MFVVKAYLPV NESFGFTADL
810 820 830 840 850
RSNTGGQAFP QCVFDHWQIL PGDPFDNSSR PSQVVAETRK RKGLKEGIPA
LDNFLDKL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK040474 mRNA Translation: BAC30601.1 AK077866 mRNA Translation: BAC37041.1 AK087985 mRNA Translation: BAC40076.1 AK145545 mRNA Translation: BAE26498.1 AK146947 mRNA Translation: BAE27556.1 AK151487 mRNA Translation: BAE30440.1 AK151812 mRNA Translation: BAE30710.1 AK151945 mRNA Translation: BAE30820.1 AK152146 mRNA Translation: BAE30983.1 AK152447 mRNA Translation: BAE31226.1 AK152587 mRNA Translation: BAE31336.1 AK152826 mRNA Translation: BAE31527.1 AK153275 mRNA Translation: BAE31861.1 AK155325 mRNA Translation: BAE33192.1 AK159806 mRNA Translation: BAE35386.1 AK163063 mRNA Translation: BAE37177.1 AK166212 mRNA Translation: BAE38631.1 AK166596 mRNA Translation: BAE38882.1 AK166747 mRNA Translation: BAE38989.1 AK166751 mRNA Translation: BAE38992.1 AK166851 mRNA Translation: BAE39070.1 AK166968 mRNA Translation: BAE39151.1 AK167093 mRNA Translation: BAE39249.1 AK167109 mRNA Translation: BAE39257.1 AK167115 mRNA Translation: BAE39263.1 AK167221 mRNA Translation: BAE39346.1 AK168794 mRNA Translation: BAE40627.1 AK168827 mRNA Translation: BAE40654.1 AK168874 mRNA Translation: BAE40692.1 AK169013 mRNA Translation: BAE40810.1 BC007152 mRNA Translation: AAH07152.1 |
CCDSi | CCDS35993.1 |
RefSeqi | NP_031933.1, NM_007907.2 |
Genome annotation databases
Ensembli | ENSMUST00000047864; ENSMUSP00000046101; ENSMUSG00000034994 |
GeneIDi | 13629 |
KEGGi | mmu:13629 |
UCSCi | uc007gge.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK040474 mRNA Translation: BAC30601.1 AK077866 mRNA Translation: BAC37041.1 AK087985 mRNA Translation: BAC40076.1 AK145545 mRNA Translation: BAE26498.1 AK146947 mRNA Translation: BAE27556.1 AK151487 mRNA Translation: BAE30440.1 AK151812 mRNA Translation: BAE30710.1 AK151945 mRNA Translation: BAE30820.1 AK152146 mRNA Translation: BAE30983.1 AK152447 mRNA Translation: BAE31226.1 AK152587 mRNA Translation: BAE31336.1 AK152826 mRNA Translation: BAE31527.1 AK153275 mRNA Translation: BAE31861.1 AK155325 mRNA Translation: BAE33192.1 AK159806 mRNA Translation: BAE35386.1 AK163063 mRNA Translation: BAE37177.1 AK166212 mRNA Translation: BAE38631.1 AK166596 mRNA Translation: BAE38882.1 AK166747 mRNA Translation: BAE38989.1 AK166751 mRNA Translation: BAE38992.1 AK166851 mRNA Translation: BAE39070.1 AK166968 mRNA Translation: BAE39151.1 AK167093 mRNA Translation: BAE39249.1 AK167109 mRNA Translation: BAE39257.1 AK167115 mRNA Translation: BAE39263.1 AK167221 mRNA Translation: BAE39346.1 AK168794 mRNA Translation: BAE40627.1 AK168827 mRNA Translation: BAE40654.1 AK168874 mRNA Translation: BAE40692.1 AK169013 mRNA Translation: BAE40810.1 BC007152 mRNA Translation: AAH07152.1 |
CCDSi | CCDS35993.1 |
RefSeqi | NP_031933.1, NM_007907.2 |
3D structure databases
SMRi | P58252 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 199387, 88 interactors |
IntActi | P58252, 20 interactors |
MINTi | P58252 |
STRINGi | 10090.ENSMUSP00000046101 |
PTM databases
iPTMneti | P58252 |
MetOSitei | P58252 |
PhosphoSitePlusi | P58252 |
SwissPalmi | P58252 |
2D gel databases
COMPLUYEAST-2DPAGEi | P58252 |
REPRODUCTION-2DPAGEi | P58252 |
Proteomic databases
CPTACi | non-CPTAC-3912 |
EPDi | P58252 |
jPOSTi | P58252 |
MaxQBi | P58252 |
PaxDbi | P58252 |
PeptideAtlasi | P58252 |
PRIDEi | P58252 |
Protocols and materials databases
Antibodypediai | 23430, 528 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000047864; ENSMUSP00000046101; ENSMUSG00000034994 |
GeneIDi | 13629 |
KEGGi | mmu:13629 |
UCSCi | uc007gge.2, mouse |
Organism-specific databases
CTDi | 1938 |
MGIi | MGI:95288, Eef2 |
Phylogenomic databases
eggNOGi | KOG0469, Eukaryota |
GeneTreei | ENSGT00940000154662 |
HOGENOMi | CLU_002794_11_1_1 |
InParanoidi | P58252 |
OMAi | GVMTQTE |
OrthoDBi | 140796at2759 |
PhylomeDBi | P58252 |
TreeFami | TF300575 |
Enzyme and pathway databases
Reactomei | R-MMU-156902, Peptide chain elongation R-MMU-5358493, Synthesis of diphthamide-EEF2 R-MMU-6798695, Neutrophil degranulation R-MMU-8876725, Protein methylation |
Miscellaneous databases
BioGRID-ORCSi | 13629, 10 hits in 18 CRISPR screens |
ChiTaRSi | Eef2, mouse |
PROi | PR:P58252 |
RNActi | P58252, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000034994, Expressed in male reproductive system and 324 other tissues |
Genevisiblei | P58252, MM |
Family and domain databases
Gene3Di | 3.30.230.10, 1 hit |
InterProi | View protein in InterPro IPR041095, EFG_II IPR035647, EFG_III/V IPR000640, EFG_V-like IPR004161, EFTu-like_2 IPR031157, G_TR_CS IPR027417, P-loop_NTPase IPR020568, Ribosomal_S5_D2-typ_fold IPR014721, Ribosomal_S5_D2-typ_fold_subgr IPR005225, Small_GTP-bd_dom IPR000795, T_Tr_GTP-bd_dom IPR009000, Transl_B-barrel_sf IPR005517, Transl_elong_EFG/EF2_IV |
Pfami | View protein in Pfam PF00679, EFG_C, 1 hit PF14492, EFG_III, 1 hit PF03764, EFG_IV, 1 hit PF00009, GTP_EFTU, 1 hit PF03144, GTP_EFTU_D2, 1 hit |
PRINTSi | PR00315, ELONGATNFCT |
SMARTi | View protein in SMART SM00838, EFG_C, 1 hit SM00889, EFG_IV, 1 hit |
SUPFAMi | SSF50447, SSF50447, 1 hit SSF52540, SSF52540, 1 hit SSF54211, SSF54211, 1 hit SSF54980, SSF54980, 2 hits |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS00301, G_TR_1, 1 hit PS51722, G_TR_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | EF2_MOUSE | |
Accessioni | P58252Primary (citable) accession number: P58252 Secondary accession number(s): Q544E4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 29, 2001 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 10, 2021 | |
This is version 184 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families