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Protein

Elongation factor 2

Gene

Eef2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi26 – 33GTPBy similarity8
Nucleotide bindingi104 – 108GTPBy similarity5
Nucleotide bindingi158 – 161GTPBy similarity4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionElongation factor
Biological processProtein biosynthesis
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-156902 Peptide chain elongation
R-MMU-5358493 Synthesis of diphthamide-EEF2
R-MMU-6798695 Neutrophil degranulation
R-MMU-8876725 Protein methylation

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor 2
Short name:
EF-2
Gene namesi
Name:Eef2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:95288 Eef2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved2 Publications
ChainiPRO_00000910022 – 858Elongation factor 2Add BLAST857

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei54PhosphothreonineBy similarity1
Modified residuei57Phosphothreonine; by EEF2KBy similarity1
Modified residuei59PhosphothreonineBy similarity1
Modified residuei152N6-succinyllysineCombined sources1
Modified residuei235N6-acetyllysineBy similarity1
Modified residuei239N6-acetyllysine; alternateCombined sources1
Cross-linki239Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity
Modified residuei265Phosphotyrosine; by CSKBy similarity1
Modified residuei272N6-acetyllysine; alternateCombined sources1
Modified residuei272N6-succinyllysine; alternateCombined sources1
Modified residuei275N6-acetyllysineCombined sources1
Cross-linki322Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Modified residuei325PhosphoserineBy similarity1
Modified residuei373Phosphotyrosine; by CSKBy similarity1
Modified residuei435PhosphothreonineBy similarity1
Modified residuei439N6-acetyllysineCombined sources1
Modified residuei445N6-acetyllysineCombined sources1
Modified residuei502PhosphoserineBy similarity1
Modified residuei525N6,N6,N6-trimethyllysine; by EEF2KMTBy similarity1
Cross-linki529Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Modified residuei572N6-succinyllysineCombined sources1
Modified residuei595Phosphoserine; by CDK2By similarity1
Modified residuei619N6-acetyllysineCombined sources1
Modified residuei715DiphthamideBy similarity1

Post-translational modificationi

Phosphorylation by EF-2 kinase completely inactivates EF-2; it requires prior phosphorylation by CDK2 at Ser-595 during mitotic prometaphase. Phosphorylation by CSK promotes SUMOylation, proteolytic cleavage, and nuclear translocation if the C-terminal fragment.By similarity
Diphthamide is 2-[3-carboxyamido-3-(trimethyl-ammonio)propyl]histidine. Diphthamide can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A, thus arresting protein synthesis (By similarity).By similarity
ISGylated.1 Publication
Proteolytically processed at two sites following phosphorylation by CSK.By similarity
SUMOylated following phosphorylation by CSK, promotes proteolytic cleavage.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP58252
MaxQBiP58252
PaxDbiP58252
PeptideAtlasiP58252
PRIDEiP58252

2D gel databases

COMPLUYEAST-2DPAGEiP58252
REPRODUCTION-2DPAGEiP58252

PTM databases

iPTMnetiP58252
PhosphoSitePlusiP58252
SwissPalmiP58252

Expressioni

Gene expression databases

BgeeiENSMUSG00000034994
CleanExiMM_EEF2
GenevisibleiP58252 MM

Interactioni

Subunit structurei

Component of the mRNA surveillance SURF complex, at least composed of ERF1, ERF3 (ERF3A or ERF3B), EEF2, UPF1/RENT1, SMG1, SMG8 and SMG9. Interacts with RBPMS2.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi199387, 16 interactors
IntActiP58252, 17 interactors
MINTiP58252
STRINGi10090.ENSMUSP00000046101

Structurei

3D structure databases

ProteinModelPortaliP58252
SMRiP58252
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini17 – 362tr-type GPROSITE-ProRule annotationAdd BLAST346

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0469 Eukaryota
COG0480 LUCA
GeneTreeiENSGT00900000141114
HOGENOMiHOG000231589
HOVERGENiHBG001838
InParanoidiP58252
KOiK03234
OMAiEHLISGM
OrthoDBiEOG091G0A2J
PhylomeDBiP58252
TreeFamiTF300575

Family and domain databases

Gene3Di3.30.230.10, 2 hits
InterProiView protein in InterPro
IPR035647 EFG_III/V
IPR000640 EFG_V-like
IPR004161 EFTu-like_2
IPR031157 G_TR_CS
IPR027417 P-loop_NTPase
IPR020568 Ribosomal_S5_D2-typ_fold
IPR014721 Ribosomal_S5_D2-typ_fold_subgr
IPR005225 Small_GTP-bd_dom
IPR000795 TF_GTP-bd_dom
IPR009000 Transl_B-barrel_sf
IPR005517 Transl_elong_EFG/EF2_IV
PfamiView protein in Pfam
PF00679 EFG_C, 1 hit
PF03764 EFG_IV, 1 hit
PF00009 GTP_EFTU, 1 hit
PF03144 GTP_EFTU_D2, 1 hit
PRINTSiPR00315 ELONGATNFCT
SMARTiView protein in SMART
SM00838 EFG_C, 1 hit
SM00889 EFG_IV, 1 hit
SUPFAMiSSF50447 SSF50447, 1 hit
SSF52540 SSF52540, 1 hit
SSF54211 SSF54211, 1 hit
SSF54980 SSF54980, 2 hits
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS00301 G_TR_1, 1 hit
PS51722 G_TR_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P58252-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVNFTVDQIR AIMDKKANIR NMSVIAHVDH GKSTLTDSLV CKAGIIASAR
60 70 80 90 100
AGETRFTDTR KDEQERCITI KSTAISLFYE LSENDLNFIK QSKDGSGFLI
110 120 130 140 150
NLIDSPGHVD FSSEVTAALR VTDGALVVVD CVSGVCVQTE TVLRQAIAER
160 170 180 190 200
IKPVLMMNKM DRALLELQLE PEELYQTFQR IVENVNVIIS TYGEGESGPM
210 220 230 240 250
GNIMIDPVLG TVGFGSGLHG WAFTLKQFAE MYVAKFAAKG EGQLSAAERA
260 270 280 290 300
KKVEDMMKKL WGDRYFDPAN GKFSKSANSP DGKKLPRTFC QLILDPIFKV
310 320 330 340 350
FDAIMNFRKE ETAKLIEKLD IKLDSEDKDK EGKPLLKAVM RRWLPAGDAL
360 370 380 390 400
LQMITIHLPS PVTAQKYRCE LLYEGPPDDE AAMGIKSCDP KGPLMMYISK
410 420 430 440 450
MVPTSDKGRF YAFGRVFSGV VSTGLKVRIM GPNYTPGKKE DLYLKPIQRT
460 470 480 490 500
ILMMGRYVEP IEDVPCGNIV GLVGVDQFLV KTGTITTFEH AHNMRVMKFS
510 520 530 540 550
VSPVVRVAVE AKNPADLPKL VEGLKRLAKS DPMVQCIIEE SGEHIIAGAG
560 570 580 590 600
ELHLEICLKD LEEDHACIPI KKSDPVVSYR ETVSEESNVL CLSKSPNKHN
610 620 630 640 650
RLYMKARPFP DGLAEDIDKG EVSARQELKA RARYLAEKYE WDVAEARKIW
660 670 680 690 700
CFGPDGTGPN ILTDITKGVQ YLNEIKDSVV AGFQWATKEG ALCEENMRGV
710 720 730 740 750
RFDVHDVTLH ADAIHRGGGQ IIPTARRCLY ASVLTAQPRL MEPIYLVEIQ
760 770 780 790 800
CPEQVVGGIY GVLNRKRGHV FEESQVAGTP MFVVKAYLPV NESFGFTADL
810 820 830 840 850
RSNTGGQAFP QCVFDHWQIL PGDPFDNSSR PSQVVAETRK RKGLKEGIPA

LDNFLDKL
Length:858
Mass (Da):95,314
Last modified:January 23, 2007 - v2
Checksum:i0569DFFB9B1DB5F6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK040474 mRNA Translation: BAC30601.1
AK077866 mRNA Translation: BAC37041.1
AK087985 mRNA Translation: BAC40076.1
AK145545 mRNA Translation: BAE26498.1
AK146947 mRNA Translation: BAE27556.1
AK151487 mRNA Translation: BAE30440.1
AK151812 mRNA Translation: BAE30710.1
AK151945 mRNA Translation: BAE30820.1
AK152146 mRNA Translation: BAE30983.1
AK152447 mRNA Translation: BAE31226.1
AK152587 mRNA Translation: BAE31336.1
AK152826 mRNA Translation: BAE31527.1
AK153275 mRNA Translation: BAE31861.1
AK155325 mRNA Translation: BAE33192.1
AK159806 mRNA Translation: BAE35386.1
AK163063 mRNA Translation: BAE37177.1
AK166212 mRNA Translation: BAE38631.1
AK166596 mRNA Translation: BAE38882.1
AK166747 mRNA Translation: BAE38989.1
AK166751 mRNA Translation: BAE38992.1
AK166851 mRNA Translation: BAE39070.1
AK166968 mRNA Translation: BAE39151.1
AK167093 mRNA Translation: BAE39249.1
AK167109 mRNA Translation: BAE39257.1
AK167115 mRNA Translation: BAE39263.1
AK167221 mRNA Translation: BAE39346.1
AK168794 mRNA Translation: BAE40627.1
AK168827 mRNA Translation: BAE40654.1
AK168874 mRNA Translation: BAE40692.1
AK169013 mRNA Translation: BAE40810.1
BC007152 mRNA Translation: AAH07152.1
CCDSiCCDS35993.1
RefSeqiNP_031933.1, NM_007907.2
UniGeneiMm.22584
Mm.266704
Mm.2903
Mm.29203
Mm.326799
Mm.482883
Mm.486637
Mm.95452

Genome annotation databases

EnsembliENSMUST00000047864; ENSMUSP00000046101; ENSMUSG00000034994
GeneIDi13629
KEGGimmu:13629
UCSCiuc007gge.2 mouse

Similar proteinsi

Entry informationi

Entry nameiEF2_MOUSE
AccessioniPrimary (citable) accession number: P58252
Secondary accession number(s): Q544E4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 29, 2001
Last sequence update: January 23, 2007
Last modified: June 20, 2018
This is version 166 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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