UniProtKB - P57785 (LFTY2_MOUSE)
Protein
Left-right determination factor 2
Gene
Lefty2
Organism
Mus musculus (Mouse)
Status
Functioni
Required for left-right asymmetry determination of organ systems in mammals.
GO - Molecular functioni
- cytokine activity Source: GO_Central
- growth factor activity Source: UniProtKB-KW
- transforming growth factor beta receptor binding Source: InterPro
GO - Biological processi
- BMP signaling pathway Source: GO_Central
- multicellular organism development Source: UniProtKB-KW
- positive regulation of pathway-restricted SMAD protein phosphorylation Source: GO_Central
- SMAD protein signal transduction Source: GO_Central
Keywordsi
Molecular function | Cytokine, Developmental protein, Growth factor |
Enzyme and pathway databases
Reactomei | R-MMU-114608, Platelet degranulation |
Names & Taxonomyi
Protein namesi | Recommended name: Left-right determination factor 2Alternative name(s): Left-right determination factor B Protein lefty-2 Protein lefty-B |
Gene namesi | Name:Lefty2 Synonyms:Leftb |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2443573, Lefty2 |
Subcellular locationi
Extracellular region or secreted
Extracellular region or secreted
- extracellular region Source: Reactome
- extracellular space Source: GO_Central
Keywords - Cellular componenti
SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 21 | By similarityAdd BLAST | 21 | |
PropeptideiPRO_0000033812 | 22 – 77 | Or 135Sequence analysisAdd BLAST | 56 | |
ChainiPRO_0000033813 | 78 – 368 | Left-right determination factor 2Add BLAST | 291 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 158 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 253 ↔ 266 | By similarity | ||
Disulfide bondi | 265 ↔ 318 | By similarity | ||
Disulfide bondi | 295 ↔ 353 | By similarity | ||
Disulfide bondi | 299 ↔ 355 | By similarity |
Post-translational modificationi
The processing of the protein may also occur at the second R-X-X-R site located at AA 132-135. Processing appears to be regulated in a cell-type specific manner.
Keywords - PTMi
Cleavage on pair of basic residues, Disulfide bond, GlycoproteinProteomic databases
PaxDbi | P57785 |
PeptideAtlasi | P57785 |
PRIDEi | P57785 |
ProteomicsDBi | 252466 |
PTM databases
GlyGeni | P57785, 1 site |
iPTMneti | P57785 |
PhosphoSitePlusi | P57785 |
Expressioni
Developmental stagei
At the primitive streak stage (7.0 dpc), expressed in the emerging mesoderm. By 8.0 dpc, expressed exclusively on the left side of developing embryos with expression predominantly in the lateral-plate mesoderm (LPM). Weak expression in the prospective floor plate (PFP).
Gene expression databases
Bgeei | ENSMUSG00000066652, Expressed in embryo and 67 other tissues |
Genevisiblei | P57785, MM |
Interactioni
GO - Molecular functioni
- cytokine activity Source: GO_Central
- growth factor activity Source: UniProtKB-KW
- transforming growth factor beta receptor binding Source: InterPro
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000082952 |
Miscellaneous databases
RNActi | P57785, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the TGF-beta family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG3900, Eukaryota |
GeneTreei | ENSGT00390000010056 |
HOGENOMi | CLU_064098_0_0_1 |
InParanoidi | P57785 |
OMAi | CCREEHY |
OrthoDBi | 751948at2759 |
PhylomeDBi | P57785 |
TreeFami | TF106462 |
Family and domain databases
Gene3Di | 2.10.90.10, 1 hit |
InterProi | View protein in InterPro IPR029034, Cystine-knot_cytokine IPR003942, LRDF IPR001839, TGF-b_C IPR001111, TGF-b_propeptide IPR015615, TGF-beta-rel IPR017948, TGFb_CS |
PANTHERi | PTHR11848, PTHR11848, 1 hit |
Pfami | View protein in Pfam PF00019, TGF_beta, 1 hit PF00688, TGFb_propeptide, 1 hit |
PIRSFi | PIRSF037402, TGFb4, 1 hit |
PRINTSi | PR01427, TGFBETA4 |
SMARTi | View protein in SMART SM00204, TGFB, 1 hit |
SUPFAMi | SSF57501, SSF57501, 1 hit |
PROSITEi | View protein in PROSITE PS00250, TGF_BETA_1, 1 hit PS51362, TGF_BETA_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P57785-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKSLWLCWAL WVLPLAGPGA AMTEEQVLSS LLQQLQLSQA PTLDSADVEE
60 70 80 90 100
MAIPTHVRSQ YVALLQGSHA DRSRGKRFSQ NFREVAGRFL MSETSTHLLV
110 120 130 140 150
FGMEQRLPPN SELVQAVLRL FQEPVPRTAL RRFERLSPHS ARARVTIEWL
160 170 180 190 200
RVREDGSNRT ALIDSRLVSI HESGWKAFDV TEAVNFWQQL SRPRQPLLLQ
210 220 230 240 250
VSVQREHLGP GTWSAHKLVR FAAQGTPDGK GQGEPQLELH TLDLKDYGAQ
260 270 280 290 300
GNCDPEVPVT EGTRCCRQEM YLDLQGMKWA ENWILEPPGF LTYECVGSCL
310 320 330 340 350
QLPESLTIGW PFLGPRQCVA SEMTSLPMIV SVKEGGRTRP QVVSLPNMRV
360
QTCSCASDGA LIPRGIDL
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 82 | F → L (PubMed:9348041).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK031621 mRNA Translation: BAC27483.1 BC066224 mRNA Translation: AAH66224.1 |
CCDSi | CCDS15575.1 |
RefSeqi | NP_796073.1, NM_177099.4 |
Genome annotation databases
Ensembli | ENSMUST00000085797; ENSMUSP00000082952; ENSMUSG00000066652 |
GeneIDi | 320202 |
KEGGi | mmu:320202 |
UCSCi | uc007dwu.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK031621 mRNA Translation: BAC27483.1 BC066224 mRNA Translation: AAH66224.1 |
CCDSi | CCDS15575.1 |
RefSeqi | NP_796073.1, NM_177099.4 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000082952 |
PTM databases
GlyGeni | P57785, 1 site |
iPTMneti | P57785 |
PhosphoSitePlusi | P57785 |
Proteomic databases
PaxDbi | P57785 |
PeptideAtlasi | P57785 |
PRIDEi | P57785 |
ProteomicsDBi | 252466 |
Genome annotation databases
Ensembli | ENSMUST00000085797; ENSMUSP00000082952; ENSMUSG00000066652 |
GeneIDi | 320202 |
KEGGi | mmu:320202 |
UCSCi | uc007dwu.1, mouse |
Organism-specific databases
CTDi | 7044 |
MGIi | MGI:2443573, Lefty2 |
Phylogenomic databases
eggNOGi | KOG3900, Eukaryota |
GeneTreei | ENSGT00390000010056 |
HOGENOMi | CLU_064098_0_0_1 |
InParanoidi | P57785 |
OMAi | CCREEHY |
OrthoDBi | 751948at2759 |
PhylomeDBi | P57785 |
TreeFami | TF106462 |
Enzyme and pathway databases
Reactomei | R-MMU-114608, Platelet degranulation |
Miscellaneous databases
BioGRID-ORCSi | 320202, 0 hits in 53 CRISPR screens |
PROi | PR:P57785 |
RNActi | P57785, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000066652, Expressed in embryo and 67 other tissues |
Genevisiblei | P57785, MM |
Family and domain databases
Gene3Di | 2.10.90.10, 1 hit |
InterProi | View protein in InterPro IPR029034, Cystine-knot_cytokine IPR003942, LRDF IPR001839, TGF-b_C IPR001111, TGF-b_propeptide IPR015615, TGF-beta-rel IPR017948, TGFb_CS |
PANTHERi | PTHR11848, PTHR11848, 1 hit |
Pfami | View protein in Pfam PF00019, TGF_beta, 1 hit PF00688, TGFb_propeptide, 1 hit |
PIRSFi | PIRSF037402, TGFb4, 1 hit |
PRINTSi | PR01427, TGFBETA4 |
SMARTi | View protein in SMART SM00204, TGFB, 1 hit |
SUPFAMi | SSF57501, SSF57501, 1 hit |
PROSITEi | View protein in PROSITE PS00250, TGF_BETA_1, 1 hit PS51362, TGF_BETA_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | LFTY2_MOUSE | |
Accessioni | P57785Primary (citable) accession number: P57785 Secondary accession number(s): Q8BMF7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 21, 2001 |
Last sequence update: | February 6, 2007 | |
Last modified: | April 7, 2021 | |
This is version 132 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families