UniProtKB - P57771 (RGS8_HUMAN)
Protein
Regulator of G-protein signaling 8
Gene
RGS8
Organism
Homo sapiens (Human)
Status
Functioni
Regulates G protein-coupled receptor signaling cascades, including signaling via muscarinic acetylcholine receptor CHRM2 and dopamine receptor DRD2 (By similarity). Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound form (PubMed:18434541). Modulates the activity of potassium channels that are activated in response to DRD2 and CHRM2 signaling (By similarity).By similarity1 Publication
GO - Molecular functioni
- GTPase activator activity Source: UniProtKB
GO - Biological processi
- G protein-coupled acetylcholine receptor signaling pathway Source: UniProtKB
- G protein-coupled receptor signaling pathway Source: Reactome
- negative regulation of signal transduction Source: UniProtKB-KW
- positive regulation of GTPase activity Source: UniProtKB
- regulation of dopamine receptor signaling pathway Source: UniProtKB
Keywordsi
Molecular function | GTPase activation, Signal transduction inhibitor |
Enzyme and pathway databases
PathwayCommonsi | P57771 |
Reactomei | R-HSA-418594, G alpha (i) signalling events |
Names & Taxonomyi
Protein namesi | Recommended name: Regulator of G-protein signaling 8Short name: RGS8 |
Gene namesi | Name:RGS8 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:16810, RGS8 |
MIMi | 607189, gene |
neXtProti | NX_P57771 |
VEuPathDBi | HostDB:ENSG00000135824.12 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Plasma membrane
- Cell membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
Other locations
- Membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
- Perikaryon By similarity
- dendrite By similarity
Note: Detected in Purkinje cell soma and dendrites. Associated with Purkinje cell membranes. Not detected in Purkinje cell nuclei. Detected in the nucleus after heterologous expression. Recruited to the cell membrane in the presence of GNAO1.By similarity
Nucleus
- nucleus Source: UniProtKB
Plasma Membrane
- extrinsic component of cytoplasmic side of plasma membrane Source: UniProtKB
- neuronal cell body membrane Source: UniProtKB
- plasma membrane Source: Reactome
Other locations
- dendrite Source: UniProtKB
- perikaryon Source: UniProtKB-SubCell
- synapse Source: GOC
Keywords - Cellular componenti
Cell membrane, Cell projection, Membrane, NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 85397 |
OpenTargetsi | ENSG00000135824 |
PharmGKBi | PA34379 |
Miscellaneous databases
Pharosi | P57771, Tchem |
Chemistry databases
ChEMBLi | CHEMBL2034803 |
GuidetoPHARMACOLOGYi | 2813 |
Genetic variation databases
BioMutai | RGS8 |
DMDMi | 13124465 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000204199 | 1 – 180 | Regulator of G-protein signaling 8Add BLAST | 180 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 26 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MassIVEi | P57771 |
PaxDbi | P57771 |
PeptideAtlasi | P57771 |
PRIDEi | P57771 |
ProteomicsDBi | 57032 [P57771-1] 57033 [P57771-2] |
PTM databases
iPTMneti | P57771 |
PhosphoSitePlusi | P57771 |
Expressioni
Gene expression databases
Bgeei | ENSG00000135824, Expressed in caudate nucleus and 97 other tissues |
ExpressionAtlasi | P57771, baseline and differential |
Genevisiblei | P57771, HS |
Organism-specific databases
HPAi | ENSG00000135824, Tissue enhanced (brain, thyroid gland) |
Interactioni
Subunit structurei
Interacts with GNAO1 (By similarity).
Interacts with GNAI3.
By similarity1 PublicationBinary interactionsi
Hide detailsP57771
With | #Exp. | IntAct |
---|---|---|
GOLGA2 [Q08379] | 3 | EBI-10216117,EBI-618309 |
Isoform 2 [P57771-2]
Protein-protein interaction databases
BioGRIDi | 124512, 12 interactors |
DIPi | DIP-59093N |
IntActi | P57771, 10 interactors |
STRINGi | 9606.ENSP00000258302 |
Chemistry databases
BindingDBi | P57771 |
Miscellaneous databases
RNActi | P57771, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P57771 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P57771 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 56 – 171 | RGSPROSITE-ProRule annotationAdd BLAST | 116 |
Phylogenomic databases
eggNOGi | KOG3589, Eukaryota |
GeneTreei | ENSGT00940000154304 |
HOGENOMi | CLU_059863_3_1_1 |
InParanoidi | P57771 |
OMAi | DIVNRAH |
OrthoDBi | 1588190at2759 |
PhylomeDBi | P57771 |
TreeFami | TF315837 |
Family and domain databases
CDDi | cd08711, RGS_RGS8, 1 hit |
Gene3Di | 1.10.196.10, 2 hits |
InterProi | View protein in InterPro IPR016137, RGS IPR034948, RGS8 IPR034949, RGS_RGS8 IPR036305, RGS_sf IPR024066, RGS_subdom1/3 |
PANTHERi | PTHR10845:SF147, PTHR10845:SF147, 1 hit |
Pfami | View protein in Pfam PF00615, RGS, 1 hit |
PRINTSi | PR01301, RGSPROTEIN |
SMARTi | View protein in SMART SM00315, RGS, 1 hit |
SUPFAMi | SSF48097, SSF48097, 1 hit |
PROSITEi | View protein in PROSITE PS50132, RGS, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform 1 (identifier: P57771-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAALLMPRRN KGMRTRLGCL SHKSDSCSDF TAILPDKPNR ALKRLSTEEA
60 70 80 90 100
TRWADSFDVL LSHKYGVAAF RAFLKTEFSE ENLEFWLACE EFKKTRSTAK
110 120 130 140 150
LVSKAHRIFE EFVDVQAPRE VNIDFQTREA TRKNLQEPSL TCFDQAQGKV
160 170 180
HSLMEKDSYP RFLRSKMYLD LLSQSQRRLS
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketD6RD36 | D6RD36_HUMAN | Regulator of G-protein-signaling 8 | RGS8 | 57 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_036421 | 1 – 9 | MAALLMPRR → MWNTLTRSLSDHPVGKDPQA MRTGQRQ in isoform 2. 2 Publications | 9 |
Sequence databases
Genome annotation databases
Ensembli | ENST00000258302; ENSP00000258302; ENSG00000135824 [P57771-2] ENST00000367556; ENSP00000356527; ENSG00000135824 [P57771-1] ENST00000367557; ENSP00000356528; ENSG00000135824 [P57771-1] ENST00000483095; ENSP00000426289; ENSG00000135824 [P57771-1] |
GeneIDi | 85397 |
KEGGi | hsa:85397 |
UCSCi | uc001gpm.2, human [P57771-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2IHD | X-ray | 1.70 | A | 42-173 | [»] | |
2ODE | X-ray | 1.90 | B/D | 42-180 | [»] | |
5DO9 | X-ray | 2.60 | B/D/F | 42-173 | [»] | |
SMRi | P57771 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 124512, 12 interactors |
DIPi | DIP-59093N |
IntActi | P57771, 10 interactors |
STRINGi | 9606.ENSP00000258302 |
Chemistry databases
BindingDBi | P57771 |
ChEMBLi | CHEMBL2034803 |
GuidetoPHARMACOLOGYi | 2813 |
PTM databases
iPTMneti | P57771 |
PhosphoSitePlusi | P57771 |
Genetic variation databases
BioMutai | RGS8 |
DMDMi | 13124465 |
Proteomic databases
MassIVEi | P57771 |
PaxDbi | P57771 |
PeptideAtlasi | P57771 |
PRIDEi | P57771 |
ProteomicsDBi | 57032 [P57771-1] 57033 [P57771-2] |
Protocols and materials databases
Antibodypediai | 34440, 131 antibodies |
DNASUi | 85397 |
Genome annotation databases
Ensembli | ENST00000258302; ENSP00000258302; ENSG00000135824 [P57771-2] ENST00000367556; ENSP00000356527; ENSG00000135824 [P57771-1] ENST00000367557; ENSP00000356528; ENSG00000135824 [P57771-1] ENST00000483095; ENSP00000426289; ENSG00000135824 [P57771-1] |
GeneIDi | 85397 |
KEGGi | hsa:85397 |
UCSCi | uc001gpm.2, human [P57771-1] |
Organism-specific databases
CTDi | 85397 |
DisGeNETi | 85397 |
GeneCardsi | RGS8 |
HGNCi | HGNC:16810, RGS8 |
HPAi | ENSG00000135824, Tissue enhanced (brain, thyroid gland) |
MIMi | 607189, gene |
neXtProti | NX_P57771 |
OpenTargetsi | ENSG00000135824 |
PharmGKBi | PA34379 |
VEuPathDBi | HostDB:ENSG00000135824.12 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3589, Eukaryota |
GeneTreei | ENSGT00940000154304 |
HOGENOMi | CLU_059863_3_1_1 |
InParanoidi | P57771 |
OMAi | DIVNRAH |
OrthoDBi | 1588190at2759 |
PhylomeDBi | P57771 |
TreeFami | TF315837 |
Enzyme and pathway databases
PathwayCommonsi | P57771 |
Reactomei | R-HSA-418594, G alpha (i) signalling events |
Miscellaneous databases
BioGRID-ORCSi | 85397, 5 hits in 978 CRISPR screens |
ChiTaRSi | RGS8, human |
EvolutionaryTracei | P57771 |
GeneWikii | RGS8 |
GenomeRNAii | 85397 |
Pharosi | P57771, Tchem |
PROi | PR:P57771 |
RNActi | P57771, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000135824, Expressed in caudate nucleus and 97 other tissues |
ExpressionAtlasi | P57771, baseline and differential |
Genevisiblei | P57771, HS |
Family and domain databases
CDDi | cd08711, RGS_RGS8, 1 hit |
Gene3Di | 1.10.196.10, 2 hits |
InterProi | View protein in InterPro IPR016137, RGS IPR034948, RGS8 IPR034949, RGS_RGS8 IPR036305, RGS_sf IPR024066, RGS_subdom1/3 |
PANTHERi | PTHR10845:SF147, PTHR10845:SF147, 1 hit |
Pfami | View protein in Pfam PF00615, RGS, 1 hit |
PRINTSi | PR01301, RGSPROTEIN |
SMARTi | View protein in SMART SM00315, RGS, 1 hit |
SUPFAMi | SSF48097, SSF48097, 1 hit |
PROSITEi | View protein in PROSITE PS50132, RGS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RGS8_HUMAN | |
Accessioni | P57771Primary (citable) accession number: P57771 Secondary accession number(s): B4DGL9, Q3SYD2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 21, 2001 |
Last sequence update: | February 21, 2001 | |
Last modified: | April 7, 2021 | |
This is version 161 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references