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Protein

DNA polymerase sliding clamp 1

Gene

pcn1

Organism
Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

One of the sliding clamp subunits that acts as a moving platform for DNA processing. Responsible for tethering the catalytic subunit of DNA polymerase to DNA during high-speed replication. Heterotrimer stimulates the Holliday junction resolvase Hjc. DNA polymerase I, DNA ligase and the flap endonuclease may be constitutively associated with the PCNA heterotrimer forming a scanning complex able to couple DNA synthesis and Okazaki fragment maturation.2 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding
Biological processDNA replication

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase sliding clamp 1UniRule annotation
Alternative name(s):
Proliferating cell nuclear antigen homolog 1UniRule annotation
Short name:
PCNA1UniRule annotation
Gene namesi
Name:pcn1UniRule annotation
Synonyms:pcnA-like
Ordered Locus Names:SSO0397
ORF Names:C41_008
OrganismiSulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Taxonomic identifieri273057 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
Proteomesi
  • UP000001974 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi114 – 116YIK → ELE: Loss of interaction with PCNA3, no change with PCNA2. 1 Publication3
Mutagenesisi175 – 177KRY → EED: Loss of interaction with both PCNA3 and PCNA2. 1 Publication3

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001492151 – 249DNA polymerase sliding clamp 1Add BLAST249

Proteomic databases

PRIDEiP57766

Interactioni

Subunit structurei

Forms heterodimers with PCNA2, which then recruit PCNA3; does not form homotrimers (PubMed:12535540, PubMed:18703842). The heterodimers interact with RfcS homotetramers (PubMed:12535540). Heterotrimer which circularizes head-to-tail (head is at N-terminus, tail is at C-terminus) to form a toroid; DNA passes through its center. Replication factor C (RFC) is required to load the toroid on the DNA. Heterotrimer interacts, probably via this subunit, with flap endonuclease 1 (fen) (PubMed:12535540), Hjc (PubMed:17011573), Dpo4 (PubMed:19054331), and XPF (PubMed:12675797).8 Publications

Protein-protein interaction databases

DIPiDIP-48854N
IntActiP57766, 1 interactor
STRINGi273057.SSO0397

Structurei

Secondary structure

1249
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP57766
SMRiP57766
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP57766

Family & Domainsi

Sequence similaritiesi

Belongs to the PCNA family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00488 Archaea
COG0592 LUCA
HOGENOMiHOG000270356
InParanoidiP57766
KOiK04802
OMAiNASKMKY
OrthoDBiPOG093Z0814

Family and domain databases

HAMAPiMF_00317 DNApol_clamp_arch, 1 hit
InterProiView protein in InterPro
IPR000730 Pr_cel_nuc_antig
IPR022648 Pr_cel_nuc_antig_N
PANTHERiPTHR11352:SF0 PTHR11352:SF0, 1 hit
PfamiView protein in Pfam
PF00705 PCNA_N, 1 hit

Sequencei

Sequence statusi: Complete.

P57766-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MFKIVYPNAK DFFSFINSIT NVTDSIILNF TEDGIFSRHL TEDKVLMAIM
60 70 80 90 100
RIPKDVLSEY SIDSPTSVKL DVSSVKKILS KASSKKATIE LTETDSGLKI
110 120 130 140 150
IIRDEKSGAK STIYIKAEKG QVEQLTEPKV NLAVNFTTDE SVLNVIAADV
160 170 180 190 200
TLVGEEMRIS TEEDKIKIEA GEEGKRYVAF LMKDKPLKEL SIDTSASSSY
210 220 230 240
SAEMFKDAVK GLRGFSAPTM VSFGENLPMK IDVEAVSGGH MIFWIAPRL
Length:249
Mass (Da):27,536
Last modified:February 21, 2001 - v1
Checksum:i137EB10BE2C03F77
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006641 Genomic DNA Translation: AAK40726.1
PIRiG90183
RefSeqiWP_009988793.1, NC_002754.1

Genome annotation databases

EnsemblBacteriaiAAK40726; AAK40726; SSO0397
GeneIDi27426696
KEGGisso:SSO0397
PATRICifig|273057.12.peg.393

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006641 Genomic DNA Translation: AAK40726.1
PIRiG90183
RefSeqiWP_009988793.1, NC_002754.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2HIIX-ray2.79A/X1-249[»]
2HIKX-ray3.30A/L/X1-249[»]
2IO4X-ray2.60A/C1-249[»]
2IX2X-ray2.20A1-249[»]
2IZOX-ray2.90C1-249[»]
2NTIX-ray2.50A/D/G1-249[»]
3FDSX-ray2.05C1-249[»]
ProteinModelPortaliP57766
SMRiP57766
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-48854N
IntActiP57766, 1 interactor
STRINGi273057.SSO0397

Proteomic databases

PRIDEiP57766

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK40726; AAK40726; SSO0397
GeneIDi27426696
KEGGisso:SSO0397
PATRICifig|273057.12.peg.393

Phylogenomic databases

eggNOGiarCOG00488 Archaea
COG0592 LUCA
HOGENOMiHOG000270356
InParanoidiP57766
KOiK04802
OMAiNASKMKY
OrthoDBiPOG093Z0814

Miscellaneous databases

EvolutionaryTraceiP57766

Family and domain databases

HAMAPiMF_00317 DNApol_clamp_arch, 1 hit
InterProiView protein in InterPro
IPR000730 Pr_cel_nuc_antig
IPR022648 Pr_cel_nuc_antig_N
PANTHERiPTHR11352:SF0 PTHR11352:SF0, 1 hit
PfamiView protein in Pfam
PF00705 PCNA_N, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPCNA1_SULSO
AccessioniPrimary (citable) accession number: P57766
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: February 21, 2001
Last modified: October 10, 2018
This is version 109 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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