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Protein

Ras-related protein Rab-25

Gene

RAB25

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the regulation of cell survival. Promotes invasive migration of cells in which it functions to localize and maintain integrin alpha-V/beta-1 at the tips of extending pseudopodia (PubMed:17925226). Involved in the regulation of epithelial morphogenesis through the control of CLDN4 expression and localization at tight junctions (By similarity). May selectively regulate the apical recycling pathway. Together with MYO5B regulates transcytosis (By similarity).By similarity1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi19 – 27GTP9
Nucleotide bindingi67 – 71GTPBy similarity5
Nucleotide bindingi125 – 128GTP4
Nucleotide bindingi155 – 157GTP3

GO - Molecular functioni

  • GTPase activity Source: InterPro
  • GTP binding Source: UniProtKB-KW
  • myosin V binding Source: UniProtKB

GO - Biological processi

Keywordsi

Biological processProtein transport, Transport
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-8873719 RAB geranylgeranylation

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rab-25
Alternative name(s):
CATX-8
Gene namesi
Name:RAB25Imported
Synonyms:CATX8
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000132698.13
HGNCiHGNC:18238 RAB25
MIMi612942 gene
neXtProtiNX_P57735

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasmic vesicle, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi57111
OpenTargetsiENSG00000132698
PharmGKBiPA34115

Polymorphism and mutation databases

BioMutaiRAB25
DMDMi46577696

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001212151 – 210Ras-related protein Rab-25Add BLAST210
PropeptideiPRO_0000370821211 – 213Removed in mature formSequence analysis3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi209S-geranylgeranyl cysteineBy similarity1
Modified residuei210Cysteine methyl esterSequence analysis1
Lipidationi210S-geranylgeranyl cysteineBy similarity1

Keywords - PTMi

Lipoprotein, Methylation, Prenylation

Proteomic databases

EPDiP57735
MaxQBiP57735
PaxDbiP57735
PeptideAtlasiP57735
PRIDEiP57735
ProteomicsDBi57023

PTM databases

iPTMnetiP57735
PhosphoSitePlusiP57735

Expressioni

Tissue specificityi

Expressed in ovarian epithelium (NOE) and breast tissue. Expressed in ovarian cancer; expression is increased relative to NOE cells. Expression in ovarian cancer is stage dependent, with stage III and stage IV showing higher levels than early stage cancers. Expressed in breast cancer; expression is increased relative to normal breast tissue.1 Publication

Gene expression databases

BgeeiENSG00000132698 Expressed in 150 organ(s), highest expression level in esophagus mucosa
CleanExiHS_RAB25
GenevisibleiP57735 HS

Organism-specific databases

HPAiCAB033645
HPA010872

Interactioni

Subunit structurei

Interacts with RAB11FIP1, RAB11FIP2, RAB11FIP3 and RAB11FIP4. Interacts (via the hypervariable C-terminal region) with ITGB1 (via the cytoplasmic region); the interaction is GTP-dependent. Interacts with ITGAV. Associates with the integrin alpha-V/beta-1 heterodimer.3 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi121377, 18 interactors
IntActiP57735, 4 interactors
MINTiP57735
STRINGi9606.ENSP00000354376

Structurei

Secondary structure

1213
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP57735
SMRiP57735
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP57735

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi41 – 49Effector regionBy similarity9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated

Phylogenomic databases

eggNOGiKOG0087 Eukaryota
COG1100 LUCA
GeneTreeiENSGT00760000118841
HOGENOMiHOG000233968
HOVERGENiHBG009351
InParanoidiP57735
KOiK07906
OMAiMGSDEAY
PhylomeDBiP57735
TreeFamiTF300099

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR001806 Small_GTPase
PfamiView protein in Pfam
PF00071 Ras, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51419 RAB, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P57735-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGNGTEEDYN FVFKVVLIGE SGVGKTNLLS RFTRNEFSHD SRTTIGVEFS
60 70 80 90 100
TRTVMLGTAA VKAQIWDTAG LERYRAITSA YYRGAVGALL VFDLTKHQTY
110 120 130 140 150
AVVERWLKEL YDHAEATIVV MLVGNKSDLS QAREVPTEEA RMFAENNGLL
160 170 180 190 200
FLETSALDST NVELAFETVL KEIFAKVSKQ RQNSIRTNAI TLGSAQAGQE
210
PGPGEKRACC ISL
Length:213
Mass (Da):23,496
Last modified:April 26, 2004 - v2
Checksum:i119523C3036C370E
GO

Sequence cautioni

The sequence AAF98238 differs from that shown. Reason: Frameshift at position 196.Curated
The sequence AAM69362 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti203P → L in AAF98238 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF083124 mRNA Translation: AAF98238.1 Frameshift.
AF274025 mRNA Translation: AAM69362.1 Different initiation.
AL355388 Genomic DNA No translation available.
CH471121 Genomic DNA Translation: EAW53003.1
BC004416 mRNA Translation: AAH04416.1
BC009831 mRNA Translation: AAH09831.1
BC033322 mRNA Translation: AAH33322.1
CCDSiCCDS41413.1
RefSeqiNP_065120.2, NM_020387.3
UniGeneiHs.632469

Genome annotation databases

EnsembliENST00000361084; ENSP00000354376; ENSG00000132698
GeneIDi57111
KEGGihsa:57111
UCSCiuc001fnc.4 human

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF083124 mRNA Translation: AAF98238.1 Frameshift.
AF274025 mRNA Translation: AAM69362.1 Different initiation.
AL355388 Genomic DNA No translation available.
CH471121 Genomic DNA Translation: EAW53003.1
BC004416 mRNA Translation: AAH04416.1
BC009831 mRNA Translation: AAH09831.1
BC033322 mRNA Translation: AAH33322.1
CCDSiCCDS41413.1
RefSeqiNP_065120.2, NM_020387.3
UniGeneiHs.632469

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2OILX-ray2.30A7-180[»]
3TSOX-ray1.80A/B7-180[»]
ProteinModelPortaliP57735
SMRiP57735
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi121377, 18 interactors
IntActiP57735, 4 interactors
MINTiP57735
STRINGi9606.ENSP00000354376

PTM databases

iPTMnetiP57735
PhosphoSitePlusiP57735

Polymorphism and mutation databases

BioMutaiRAB25
DMDMi46577696

Proteomic databases

EPDiP57735
MaxQBiP57735
PaxDbiP57735
PeptideAtlasiP57735
PRIDEiP57735
ProteomicsDBi57023

Protocols and materials databases

DNASUi57111
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000361084; ENSP00000354376; ENSG00000132698
GeneIDi57111
KEGGihsa:57111
UCSCiuc001fnc.4 human

Organism-specific databases

CTDi57111
DisGeNETi57111
EuPathDBiHostDB:ENSG00000132698.13
GeneCardsiRAB25
HGNCiHGNC:18238 RAB25
HPAiCAB033645
HPA010872
MIMi612942 gene
neXtProtiNX_P57735
OpenTargetsiENSG00000132698
PharmGKBiPA34115
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0087 Eukaryota
COG1100 LUCA
GeneTreeiENSGT00760000118841
HOGENOMiHOG000233968
HOVERGENiHBG009351
InParanoidiP57735
KOiK07906
OMAiMGSDEAY
PhylomeDBiP57735
TreeFamiTF300099

Enzyme and pathway databases

ReactomeiR-HSA-8873719 RAB geranylgeranylation

Miscellaneous databases

EvolutionaryTraceiP57735
GeneWikiiRAB25
GenomeRNAii57111
PROiPR:P57735
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000132698 Expressed in 150 organ(s), highest expression level in esophagus mucosa
CleanExiHS_RAB25
GenevisibleiP57735 HS

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR001806 Small_GTPase
PfamiView protein in Pfam
PF00071 Ras, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51419 RAB, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRAB25_HUMAN
AccessioniPrimary (citable) accession number: P57735
Secondary accession number(s): Q5VYA2
, Q8NG24, Q96GB1, Q9BT12
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: April 26, 2004
Last modified: September 12, 2018
This is version 171 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. SIMILARITY comments
    Index of protein domains and families
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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