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Protein

DNA polymerase III subunit epsilon

Gene

dnaQ

Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. The epsilon subunit contain the editing function and is a proofreading 3'-5' exonuclease (By similarity).By similarity

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 divalent metal cations. Magnesium or manganese.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi10Divalent metal cation 1; catalyticBy similarity1
Metal bindingi10Divalent metal cation 2; catalyticBy similarity1
Binding sitei10SubstrateBy similarity1
Metal bindingi12Divalent metal cation 1; catalyticBy similarity1
Binding sitei12SubstrateBy similarity1
Binding sitei64SubstrateBy similarity1
Active sitei159Proton acceptorBy similarity1
Metal bindingi164Divalent metal cation 1; catalyticBy similarity1
Binding sitei164SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-directed DNA polymerase, Exonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Transferase
Biological processDNA replication
LigandMagnesium, Manganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase III subunit epsilon (EC:2.7.7.7)
Gene namesi
Name:dnaQ
Ordered Locus Names:BU248
OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Taxonomic identifieri107806 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesErwiniaceaeBuchnera
Proteomesi
  • UP000001806 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001054801 – 237DNA polymerase III subunit epsilonAdd BLAST237

Interactioni

Subunit structurei

The DNA polymerase holoenzyme is a complex that contains 10 different types of subunits. These subunits are organized into 3 functionally essential subassemblies: the pol III core, the beta sliding clamp processivity factor and the clamp-loading complex. The pol III core (subunits alpha,epsilon and theta) contains the polymerase and the 3'-5' exonuclease proofreading activities. The polymerase is tethered to the template via the sliding clamp processivity factor. The clamp-loading complex assembles the beta processivity factor onto the primer template and plays a central role in the organization and communication at the replication fork. This complex contains delta, delta', psi and chi, and copies of either or both of two different DnaX proteins, gamma and tau. The composition of the holoenzyme is, therefore: (alpha,epsilon,theta)[2]-(gamma/tau)[3]-delta,delta', psi,chi-beta[4] (By similarity).By similarity

Protein-protein interaction databases

STRINGi107806.BU248

Structurei

3D structure databases

ProteinModelPortaliP57337
SMRiP57337
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

eggNOGiENOG4107RZZ Bacteria
COG0847 LUCA
HOGENOMiHOG000258616
KOiK02342
OMAiFHVYLNP

Family and domain databases

CDDicd06131 DNA_pol_III_epsilon_Ecoli_like, 1 hit
Gene3Di3.30.420.10, 1 hit
InterProiView protein in InterPro
IPR006054 DnaQ
IPR006309 DnaQ_proteo
IPR013520 Exonuclease_RNaseT/DNA_pol3
IPR012337 RNaseH-like_sf
IPR036397 RNaseH_sf
PfamiView protein in Pfam
PF00929 RNase_T, 1 hit
SMARTiView protein in SMART
SM00479 EXOIII, 1 hit
SUPFAMiSSF53098 SSF53098, 1 hit
TIGRFAMsiTIGR00573 dnaq, 1 hit
TIGR01406 dnaQ_proteo, 1 hit

Sequencei

Sequence statusi: Complete.

P57337-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNRKRTIILD TETTGINQTS LPHINHRIIE IGAVEIIDRC FTGNNFHVYI
60 70 80 90 100
QPGRSIESGA LKVHGITNKF LLDKPIFKDI ADSFLNYIKN SILVIHNASF
110 120 130 140 150
DVGFINQELE ILNKKIKINT FCSIIDTLKI ARELFPGKKN TLDALCTRYK
160 170 180 190 200
INKSHRNLHS AIVDSYLLGK LYLLMTGGQD SLFSDNTINY KENFKKLKKN
210 220 230
IQLKNNTLRI LHPTLKENDL HEKYLQYMKD KSTCLWN
Length:237
Mass (Da):27,365
Last modified:December 1, 2000 - v1
Checksum:i3E4C4F102C50EBB7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000003 Genomic DNA Translation: BAB12959.1
RefSeqiNP_240073.1, NC_002528.1
WP_010896025.1, NC_002528.1

Genome annotation databases

EnsemblBacteriaiBAB12959; BAB12959; BAB12959
GeneIDi1109688
KEGGibuc:BU248
PATRICifig|107806.10.peg.259

Similar proteinsi

Entry informationi

Entry nameiDPO3E_BUCAI
AccessioniPrimary (citable) accession number: P57337
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: March 28, 2018
This is version 102 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Buchnera aphidicola (subsp. Acyrthosiphon pisum)
    Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names

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