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Entry version 122 (26 Feb 2020)
Sequence version 1 (01 Dec 2000)
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Protein

Bifunctional aspartokinase/homoserine dehydrogenase

Gene

thrA

Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Protein inferred from homologyi <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: L-lysine biosynthesis via DAP pathway

This protein is involved in step 1 of the subpathway that synthesizes (S)-tetrahydrodipicolinate from L-aspartate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Bifunctional aspartokinase/homoserine dehydrogenase (thrA)
  2. Aspartate-semialdehyde dehydrogenase (asd)
  3. 4-hydroxy-tetrahydrodipicolinate synthase (dapA)
  4. 4-hydroxy-tetrahydrodipicolinate reductase (dapB)
This subpathway is part of the pathway L-lysine biosynthesis via DAP pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-tetrahydrodipicolinate from L-aspartate, the pathway L-lysine biosynthesis via DAP pathway and in Amino-acid biosynthesis.

Pathwayi: L-methionine biosynthesis via de novo pathway

This protein is involved in step 1 and 3 of the subpathway that synthesizes L-homoserine from L-aspartate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Bifunctional aspartokinase/homoserine dehydrogenase (thrA)
  2. Aspartate-semialdehyde dehydrogenase (asd)
  3. Bifunctional aspartokinase/homoserine dehydrogenase (thrA)
This subpathway is part of the pathway L-methionine biosynthesis via de novo pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-homoserine from L-aspartate, the pathway L-methionine biosynthesis via de novo pathway and in Amino-acid biosynthesis.

Pathwayi: L-threonine biosynthesis

This protein is involved in step 1 and 3 of the subpathway that synthesizes L-threonine from L-aspartate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Bifunctional aspartokinase/homoserine dehydrogenase (thrA)
  2. Aspartate-semialdehyde dehydrogenase (asd)
  3. Bifunctional aspartokinase/homoserine dehydrogenase (thrA)
  4. Homoserine kinase (thrB)
  5. Threonine synthase (thrC)
This subpathway is part of the pathway L-threonine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-threonine from L-aspartate, the pathway L-threonine biosynthesis and in Amino-acid biosynthesis.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes a region in the protein which binds nucleotide phosphates. It always involves more than one amino acid and includes all residues involved in nucleotide-binding.<p><a href='/help/np_bind' target='_top'>More...</a></p>Nucleotide bindingi472 – 479NADPSequence analysis8

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionKinase, Multifunctional enzyme, Oxidoreductase, Transferase
Biological processAmino-acid biosynthesis, Threonine biosynthesis
LigandATP-binding, NADP, Nucleotide-binding

Enzyme and pathway databases

UniPathway: a resource for the exploration and annotation of metabolic pathways

More...
UniPathwayi
UPA00034;UER00015
UPA00050;UER00063
UPA00050;UER00461
UPA00051;UER00462
UPA00051;UER00465

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Bifunctional aspartokinase/homoserine dehydrogenase
Short name:
AK-HD
Including the following 2 domains:
Aspartokinase (EC:2.7.2.4)
Homoserine dehydrogenase (EC:1.1.1.3)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:thrA
Ordered Locus Names:BU194
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri107806 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesErwiniaceaeBuchnera
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000001806 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000666841 – 816Bifunctional aspartokinase/homoserine dehydrogenaseAdd BLAST816

Proteomic databases

PRoteomics IDEntifications database

More...
PRIDEi
P57290

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Homotetramer.

By similarity

Protein-protein interaction databases

STRING: functional protein association networks

More...
STRINGi
107806.10038876

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
P57290

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini402 – 479ACTPROSITE-ProRule annotationAdd BLAST78

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni1 – 250AspartokinaseBy similarityAdd BLAST250
Regioni251 – 471InterfaceBy similarityAdd BLAST221
Regioni472 – 816Homoserine dehydrogenaseBy similarityAdd BLAST345

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

In the N-terminal section; belongs to the aspartokinase family.Curated
In the C-terminal section; belongs to the homoserine dehydrogenase family.Curated

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
ENOG4105CFH Bacteria
COG0460 LUCA
COG0527 LUCA

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
CLU_009116_7_1_6

KEGG Orthology (KO)

More...
KOi
K12524

Identification of Orthologs from Complete Genome Data

More...
OMAi
CNKIACS

Family and domain databases

Conserved Domains Database

More...
CDDi
cd04257 AAK_AK-HSDH, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
3.40.1160.10, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR036393 AceGlu_kinase-like_sf
IPR002912 ACT_dom
IPR041743 AK-HSDH_N
IPR001048 Asp/Glu/Uridylate_kinase
IPR005106 Asp/hSer_DH_NAD-bd
IPR001341 Asp_kinase
IPR018042 Aspartate_kinase_CS
IPR011147 Bifunc_aspartokin/hSer_DH
IPR001342 HDH_cat
IPR019811 HDH_CS
IPR036291 NAD(P)-bd_dom_sf

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF00696 AA_kinase, 1 hit
PF00742 Homoserine_dh, 1 hit
PF03447 NAD_binding_3, 1 hit

PIRSF; a whole-protein classification database

More...
PIRSFi
PIRSF000727 ThrA, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF51735 SSF51735, 1 hit
SSF53633 SSF53633, 1 hit

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR00657 asp_kinases, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS51671 ACT, 1 hit
PS00324 ASPARTOKINASE, 1 hit
PS01042 HOMOSER_DHGENASE, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P57290-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKLLKFGGTS LANAEKFLSV SSIIEENTQT DQIAVVLSAP AKITNYLVKI
60 70 80 90 100
IENTIKNNQI LETVHLAENI FMQLINNFLN IQSNFPHKEI EKIIKKEFNE
110 120 130 140 150
LKNIIQGILL LKQCPDNIRA IIISRGEILS VFIMKSILQS KNYNVTIINP
160 170 180 190 200
VKNLVAIGDN YLDSTVDISE SKKNIQNMNI NQSNIILMAG FIAGNKDKKL
210 220 230 240 250
VVLGRNGSDY SAAVLAACLD ANCCEIWTDV DGVFTSDPRK VPNARLLKSI
260 270 280 290 300
SYQEAMELSY FGAKVLHPRT IEPIAQFKIP CLIKNTNNVK SIGTLICEQN
310 320 330 340 350
CSEKDFLKGV THLDEIAMFN ISGPHIKDVG SVISRIFTMM SRGNIKILLI
360 370 380 390 400
TQSSSENKIN FCVYEHDIYK ILYLFNKEFQ LELKDGLLNP FKIKKNLSIL
410 420 430 440 450
SIVGSNIYKK HNIASKIFSV LGALKINVIA ISQGSSKHSI SLVIKKENIL
460 470 480 490 500
KAVQHVHNTL FFNKKTIHMF LIGIGGIGST LLNQILKQKQ FLDKKNIEIK
510 520 530 540 550
ICAIANSKKI LLLDNTNDLS NWKNDFKKST QKFNLELLNN LIKNNHFSNS
560 570 580 590 600
VIVDCTSSQL LSEQYVNFID NNFHVVTSNK KANTSEWNYY KKIRKSVAQT
610 620 630 640 650
GKKFLYETNV GAGLPVIETL QNLFKSGDNL ICFKGILSGS LSFIFGRLEE
660 670 680 690 700
GILLSQATRE AKELGFTEPN PCDDLSGIDV ARKLLILARE SGYNIELKDI
710 720 730 740 750
KIEPLLPNNF KIYEDTDKFL LKLKELDVYF SNKIKQALNV GNVLRFVATI
760 770 780 790 800
EQKRQFFIKL EEVNINNPLY KVKNGENALA FYTNYYQPIP LVLRGYGAGN
810
NVTASGVFSD LLRTLS
Length:816
Mass (Da):91,809
Last modified:December 1, 2000 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iCEACE5C565465884
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
BA000003 Genomic DNA Translation: BAB12911.1

NCBI Reference Sequences

More...
RefSeqi
NP_240025.1, NC_002528.1
WP_010895993.1, NC_002528.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...
EnsemblBacteriai
BAB12911; BAB12911; BAB12911

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
1109637

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
buc:BU194

Pathosystems Resource Integration Center (PATRIC)

More...
PATRICi
fig|107806.10.peg.205

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000003 Genomic DNA Translation: BAB12911.1
RefSeqiNP_240025.1, NC_002528.1
WP_010895993.1, NC_002528.1

3D structure databases

SMRiP57290
ModBaseiSearch...

Protein-protein interaction databases

STRINGi107806.10038876

Proteomic databases

PRIDEiP57290

Genome annotation databases

EnsemblBacteriaiBAB12911; BAB12911; BAB12911
GeneIDi1109637
KEGGibuc:BU194
PATRICifig|107806.10.peg.205

Phylogenomic databases

eggNOGiENOG4105CFH Bacteria
COG0460 LUCA
COG0527 LUCA
HOGENOMiCLU_009116_7_1_6
KOiK12524
OMAiCNKIACS

Enzyme and pathway databases

UniPathwayiUPA00034;UER00015
UPA00050;UER00063
UPA00050;UER00461
UPA00051;UER00462
UPA00051;UER00465

Family and domain databases

CDDicd04257 AAK_AK-HSDH, 1 hit
Gene3Di3.40.1160.10, 1 hit
InterProiView protein in InterPro
IPR036393 AceGlu_kinase-like_sf
IPR002912 ACT_dom
IPR041743 AK-HSDH_N
IPR001048 Asp/Glu/Uridylate_kinase
IPR005106 Asp/hSer_DH_NAD-bd
IPR001341 Asp_kinase
IPR018042 Aspartate_kinase_CS
IPR011147 Bifunc_aspartokin/hSer_DH
IPR001342 HDH_cat
IPR019811 HDH_CS
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF00696 AA_kinase, 1 hit
PF00742 Homoserine_dh, 1 hit
PF03447 NAD_binding_3, 1 hit
PIRSFiPIRSF000727 ThrA, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
SSF53633 SSF53633, 1 hit
TIGRFAMsiTIGR00657 asp_kinases, 1 hit
PROSITEiView protein in PROSITE
PS51671 ACT, 1 hit
PS00324 ASPARTOKINASE, 1 hit
PS01042 HOMOSER_DHGENASE, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiAKH_BUCAI
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P57290
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: February 26, 2020
This is version 122 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
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