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Protein

NADH-quinone oxidoreductase subunit G

Gene

nuoG

Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient (By similarity).By similarity

Catalytic activityi

NADH + a quinone = NAD+ + a quinol.

Cofactori

Protein has several cofactor binding sites:
  • [2Fe-2S] clusterBy similarityNote: Binds 1 [2Fe-2S] cluster per subunit.By similarity
  • [4Fe-4S] clusterBy similarityNote: Binds 3 [4Fe-4S] clusters per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi34Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi45Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi48Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi67Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi99Iron-sulfur 2 (4Fe-4S); via tele nitrogenPROSITE-ProRule annotation1
Metal bindingi103Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi106Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi112Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi151Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi154Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi157Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi201Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi228Iron-sulfur 4 (4Fe-4S)Sequence analysis1
Metal bindingi231Iron-sulfur 4 (4Fe-4S)Sequence analysis1
Metal bindingi235Iron-sulfur 4 (4Fe-4S)Sequence analysis1
Metal bindingi263Iron-sulfur 4 (4Fe-4S)Sequence analysis1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Ligand2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-quinone oxidoreductase subunit G (EC:1.6.5.11)
Alternative name(s):
NADH dehydrogenase I subunit G
NDH-1 subunit G
Gene namesi
Name:nuoG
Ordered Locus Names:BU159
OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Taxonomic identifieri107806 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesErwiniaceaeBuchnera
Proteomesi
  • UP000001806 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001185421 – 906NADH-quinone oxidoreductase subunit GAdd BLAST906

Keywords - PTMi

Quinone

Proteomic databases

PRIDEiP57257

Interactioni

Subunit structurei

Composed of 13 different subunits. Subunits NuoCD, E, F, and G constitute the peripheral sector of the complex (By similarity).By similarity

Protein-protein interaction databases

STRINGi107806.BU159

Structurei

3D structure databases

ProteinModelPortaliP57257
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 832Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd BLAST82
Domaini83 – 1224Fe-4S His(Cys)3-ligated-typePROSITE-ProRule annotationAdd BLAST40
Domaini221 – 2774Fe-4S Mo/W bis-MGD-typePROSITE-ProRule annotationAdd BLAST57

Sequence similaritiesi

Belongs to the complex I 75 kDa subunit family.Curated

Phylogenomic databases

eggNOGiENOG4107R0R Bacteria
COG1034 LUCA
HOGENOMiHOG000276177
KOiK00336
OMAiGYGYVNR

Family and domain databases

CDDicd00207 fer2, 1 hit
InterProiView protein in InterPro
IPR036010 2Fe-2S_ferredoxin-like_sf
IPR001041 2Fe-2S_ferredoxin-type
IPR006656 Mopterin_OxRdtase
IPR006963 Mopterin_OxRdtase_4Fe-4S_dom
IPR000283 NADH_UbQ_OxRdtase_75kDa_su_CS
IPR010228 NADH_UbQ_OxRdtase_Gsu
IPR019574 NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd
PfamiView protein in Pfam
PF04879 Molybdop_Fe4S4, 1 hit
PF00384 Molybdopterin, 1 hit
PF10588 NADH-G_4Fe-4S_3, 1 hit
SMARTiView protein in SMART
SM00926 Molybdop_Fe4S4, 1 hit
SM00929 NADH-G_4Fe-4S_3, 1 hit
SUPFAMiSSF54292 SSF54292, 1 hit
TIGRFAMsiTIGR01973 NuoG, 1 hit
PROSITEiView protein in PROSITE
PS51085 2FE2S_FER_2, 1 hit
PS51839 4FE4S_HC3, 1 hit
PS51669 4FE4S_MOW_BIS_MGD, 1 hit
PS00641 COMPLEX1_75K_1, 1 hit
PS00642 COMPLEX1_75K_2, 1 hit
PS00643 COMPLEX1_75K_3, 1 hit

Sequencei

Sequence statusi: Complete.

P57257-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAKIYVDSKI YNVNESDNLL QACLSVGINI PYFCWHPLLG SLGACRQCAV
60 70 80 90 100
TQYDNFQDRK GRLIMSCMTP VTDGAIISIK STESEVFRSA IVELLLTNHP
110 120 130 140 150
HDCPVCEEGG HCHLQDMTVM VKHSMRNYRF KKRTHKNQYL GPFIKHEMNR
160 170 180 190 200
CIACYRCVRY YNEYADGVDF GVYGSNNNVY FGRIEDGVLE SEHSGNLIEL
210 220 230 240 250
CPTGVFTDKT HSKKYNRKWD MQYAPGICHN CSVGCNISIG ERYGEIRRIE
260 270 280 290 300
NRYHENINHY LICDLGRFGY SHTNLNTRPK KPTYVNKYND LNVLNFNEAI
310 320 330 340 350
KIGVDFFKRY KRVIGVGSAR SSIENNFALQ ELVGKENFSN GMSNKEKECI
360 370 380 390 400
KSILEFLKNN YIYIPSLKEI ESYDVILVLG EDLTQTSSRV ALAVRQAVKK
410 420 430 440 450
KVQDIKNLYG IPKWNTSSNI HISEKFKNSL YIMHTHESKL DDISEWSYFA
460 470 480 490 500
SIDKQVNLAS SIAYEIDKSS PKVSNLDSEL KEKVLLISDR LISSKKTLII
510 520 530 540 550
SGSHSFSDSI IKASINIAKA IKFRAPDHHV GVTLLTSSVN SLGAELLGGM
560 570 580 590 600
SIESALDDLK KEKADAVIFM EYDLYRSVSE YDCEYFFKNK DNIITLDHQY
610 620 630 640 650
TQTFKKSMLS LPSTNFTESS GTVINFEGRA QRFFQVYDPN FYDKSNCLCD
660 670 680 690 700
SWKWLHTIKS KINNTEICWF NLDDVINSYA EKYSIFKQIK TNELNSNLRI
710 720 730 740 750
HGQKISRSPI RSSGRTSLRS NIDVHEPCQP KDINTMFAFS MEGYNQPNSS
760 770 780 790 800
VSNIPFAWFP GWNSPQAWNK FQVEVGRNLI SGDSGIHIFK KYEKKTDVYS
810 820 830 840 850
NIVLKNSIKE KYWNIIPYYH LFGNEELTQY SSIIQENTPL EYALIGLSDA
860 870 880 890 900
IKMGLKKDSI VEFNCLKKDY CLPVQVSKYL TEKQIGLPIG RKGFPLALVG

EKIEFL
Length:906
Mass (Da):103,761
Last modified:December 1, 2000 - v1
Checksum:iA1CBA1210C087A54
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000003 Genomic DNA Translation: BAB12877.1
RefSeqiNP_239991.1, NC_002528.1
WP_010895977.1, NC_002528.1

Genome annotation databases

EnsemblBacteriaiBAB12877; BAB12877; BAB12877
GeneIDi1109603
KEGGibuc:BU159
PATRICifig|107806.10.peg.169

Similar proteinsi

Entry informationi

Entry nameiNUOG_BUCAI
AccessioniPrimary (citable) accession number: P57257
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: May 23, 2018
This is version 106 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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