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Protein

Maleylacetoacetate isomerase

Gene

Gstz1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Probable bifunctional enzyme showing minimal glutathione-conjugating activity with ethacrynic acid and 7-chloro-4-nitrobenz-2-oxa-1, 3-diazole and maleylacetoacetate isomerase activity. Has also low glutathione peroxidase activity with t-butyl and cumene hydroperoxides (By similarity). Is able to catalyze the glutathione dependent oxygenation of dichloroacetic acid to glyoxylic acid.By similarity1 Publication

Catalytic activityi

4-maleylacetoacetate = 4-fumarylacetoacetate.1 Publication
RX + glutathione = HX + R-S-glutathione.1 Publication

Cofactori

glutathioneBy similarityNote: Glutathione is required for the MAAI activity.By similarity

Kineticsi

  1. KM=71.4 µM for dichloroacetic acid1 Publication
  2. KM=59.0 µM for glutathione1 Publication
  1. Vmax=1334 nmol/min/mg enzyme1 Publication

Pathwayi: L-phenylalanine degradation

This protein is involved in step 5 of the subpathway that synthesizes acetoacetate and fumarate from L-phenylalanine.
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Phenylalanine-4-hydroxylase (Pah)
  2. Tyrosine aminotransferase (Tat)
  3. 4-hydroxyphenylpyruvate dioxygenase (Hpd)
  4. no protein annotated in this organism
  5. Maleylacetoacetate isomerase (Gstz1)
  6. Fumarylacetoacetase (Fah)
This subpathway is part of the pathway L-phenylalanine degradation, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes acetoacetate and fumarate from L-phenylalanine, the pathway L-phenylalanine degradation and in Amino-acid degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei45GlutathioneBy similarity1
Binding sitei59Glutathione; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei111GlutathioneBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase, Multifunctional enzyme, Transferase
Biological processPhenylalanine catabolism, Tyrosine catabolism

Enzyme and pathway databases

ReactomeiR-RNO-156590 Glutathione conjugation
R-RNO-204174 Regulation of pyruvate dehydrogenase (PDH) complex
R-RNO-71182 Phenylalanine and tyrosine catabolism
UniPathwayiUPA00139; UER00340

Names & Taxonomyi

Protein namesi
Recommended name:
Maleylacetoacetate isomerase (EC:5.2.1.2)
Short name:
MAAI
Alternative name(s):
GSTZ1-1
Glutathione S-transferase zeta 1 (EC:2.5.1.18)
Gene namesi
Name:Gstz1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 6

Organism-specific databases

RGDi1589363 Gstz1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001860241 – 216Maleylacetoacetate isomeraseAdd BLAST216

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei57N6-succinyllysineBy similarity1
Modified residuei136PhosphothreonineBy similarity1
Modified residuei137PhosphoserineCombined sources1
Modified residuei177N6-succinyllysineBy similarity1
Modified residuei181PhosphoserineCombined sources1

Post-translational modificationi

The N-terminus is blocked.

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP57113
PRIDEiP57113

PTM databases

iPTMnetiP57113
PhosphoSitePlusiP57113

Expressioni

Gene expression databases

BgeeiENSRNOG00000047708
ExpressionAtlasiP57113 baseline and differential
GenevisibleiP57113 RN

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000065390

Structurei

3D structure databases

ProteinModelPortaliP57113
SMRiP57113
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 87GST N-terminalAdd BLAST84
Domaini92 – 212GST C-terminalAdd BLAST121

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni14 – 19Glutathione bindingBy similarity6
Regioni71 – 72Glutathione bindingBy similarity2
Regioni115 – 117Glutathione bindingBy similarity3

Sequence similaritiesi

Belongs to the GST superfamily. Zeta family.Curated

Phylogenomic databases

eggNOGiKOG0868 Eukaryota
COG0625 LUCA
GeneTreeiENSGT00390000006580
HOVERGENiHBG001501
InParanoidiP57113
KOiK01800
OMAiCRQPDTP
OrthoDBiEOG091G0QFQ
PhylomeDBiP57113

Family and domain databases

CDDicd03191 GST_C_Zeta, 1 hit
cd03042 GST_N_Zeta, 1 hit
InterProiView protein in InterPro
IPR010987 Glutathione-S-Trfase_C-like
IPR036282 Glutathione-S-Trfase_C_sf
IPR004045 Glutathione_S-Trfase_N
IPR004046 GST_C
IPR005955 GST_Zeta
IPR034330 GST_Zeta_C
IPR034333 GST_Zeta_N
IPR036249 Thioredoxin-like_sf
PfamiView protein in Pfam
PF14497 GST_C_3, 1 hit
PF02798 GST_N, 1 hit
SUPFAMiSSF47616 SSF47616, 1 hit
SSF52833 SSF52833, 1 hit
TIGRFAMsiTIGR01262 maiA, 1 hit
PROSITEiView protein in PROSITE
PS50405 GST_CTER, 1 hit
PS50404 GST_NTER, 1 hit

Sequencei

Sequence statusi: Complete.

P57113-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQAGKPVLYS YFRSSCSWRV RIALALKGID YEIVPINLIK DGGQQFSEEF
60 70 80 90 100
QTLNPMKQVP ALKIDGITIG QSLAILEYLE ETRPIPRLLP QDPQKRAIVR
110 120 130 140 150
MISDLIASGI QPLQNLSVLK QVGQENQMPW AQKAITSGFN ALEKILQSTA
160 170 180 190 200
GKYCVGDEVS MADVCLAPQV ANAERFKVDL SPYPTISHIN KALLALEAFQ
210
VSHPCRQPDT PAELRT
Length:216
Mass (Da):23,961
Last modified:October 19, 2011 - v2
Checksum:i3E02041A8E3749A3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FJ179410 mRNA Translation: ACI32127.1
CH473982 Genomic DNA Translation: EDL81627.1
BC158833 mRNA Translation: AAI58834.1
RefSeqiNP_001102915.1, NM_001109445.1
UniGeneiRn.216913

Genome annotation databases

EnsembliENSRNOT00000072215; ENSRNOP00000065390; ENSRNOG00000047708
GeneIDi681913
KEGGirno:681913

Similar proteinsi

Entry informationi

Entry nameiMAAI_RAT
AccessioniPrimary (citable) accession number: P57113
Secondary accession number(s): B0BNI1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: October 19, 2011
Last modified: May 23, 2018
This is version 109 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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