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UniProtKB - P57110 (ATS8_MOUSE)
Protein
A disintegrin and metalloproteinase with thrombospondin motifs 8
Gene
Adamts8
Organism
Mus musculus (Mouse)
Status
Functioni
Has anti-angiogenic properties.
By similarityCofactori
Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 378 | Zinc; catalyticBy similarity | 1 | |
Active sitei | 379 | PROSITE-ProRule annotation | 1 | |
Metal bindingi | 382 | Zinc; catalyticBy similarity | 1 | |
Metal bindingi | 388 | Zinc; catalyticBy similarity | 1 |
GO - Molecular functioni
- heparin binding Source: UniProtKB-KW
- metalloendopeptidase activity Source: GO_Central
- zinc ion binding Source: InterPro
GO - Biological processi
- extracellular matrix organization Source: GO_Central
Keywordsi
Molecular function | Heparin-binding, Hydrolase, Metalloprotease, Protease |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-MMU-5173214, O-glycosylation of TSR domain-containing proteins |
Protein family/group databases
MEROPSi | M12.226 |
Names & Taxonomyi
Protein namesi | Recommended name: A disintegrin and metalloproteinase with thrombospondin motifs 8 (EC:3.4.24.-)Short name: ADAM-TS 8 Short name: ADAM-TS8 Short name: ADAMTS-8 Alternative name(s): METH-2 |
Gene namesi | Name:Adamts8 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1353468, Adamts8 |
Subcellular locationi
Extracellular region or secreted
- extracellular matrix By similarity
Extracellular region or secreted
- extracellular region Source: UniProtKB-KW
Other locations
- collagen-containing extracellular matrix Source: InterPro
- extracellular matrix Source: GO_Central
Keywords - Cellular componenti
Extracellular matrix, SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 28 | Sequence analysisAdd BLAST | 28 | |
PropeptideiPRO_0000029180 | 29 – 228 | By similarityAdd BLAST | 200 | |
ChainiPRO_0000029181 | 229 – 905 | A disintegrin and metalloproteinase with thrombospondin motifs 8Add BLAST | 677 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 309 ↔ 362 | By similarity | ||
Disulfide bondi | 338 ↔ 344 | By similarity | ||
Disulfide bondi | 356 ↔ 439 | By similarity | ||
Disulfide bondi | 394 ↔ 423 | By similarity | ||
Glycosylationi | 415 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 478 ↔ 502 | By similarity | ||
Glycosylationi | 480 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 487 ↔ 523 | By similarity | ||
Glycosylationi | 506 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 517 ↔ 528 | By similarity | ||
Disulfide bondi | 554 ↔ 591 | By similarity | ||
Disulfide bondi | 558 ↔ 596 | By similarity | ||
Disulfide bondi | 569 ↔ 581 | By similarity | ||
Glycosylationi | 615 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Post-translational modificationi
The precursor is cleaved by a furin endopeptidase.By similarity
Glycosylated. Can be O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X2-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS family members. Also can be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs, and N-glycosylated. These other glycosylations can also facilitate secretion (By similarity).By similarity
Keywords - PTMi
Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, ZymogenProteomic databases
PaxDbi | P57110 |
PRIDEi | P57110 |
ProteomicsDBi | 273587 |
PTM databases
GlyGeni | P57110, 4 sites |
PhosphoSitePlusi | P57110 |
Expressioni
Tissue specificityi
Expressed specifically in adult lung and heart and low expression during mouse development.
Interactioni
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000069644 |
Miscellaneous databases
RNActi | P57110, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 234 – 444 | Peptidase M12BPROSITE-ProRule annotationAdd BLAST | 211 | |
Domaini | 453 – 541 | DisintegrinAdd BLAST | 89 | |
Domaini | 542 – 597 | TSP type-1 1PROSITE-ProRule annotationAdd BLAST | 56 | |
Domaini | 848 – 904 | TSP type-1 2PROSITE-ProRule annotationAdd BLAST | 57 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 139 – 163 | DisorderedSequence analysisAdd BLAST | 25 | |
Regioni | 186 – 225 | DisorderedSequence analysisAdd BLAST | 40 | |
Regioni | 706 – 847 | SpacerAdd BLAST | 142 | |
Regioni | 877 – 905 | DisorderedSequence analysisAdd BLAST | 29 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 190 – 218 | Basic and acidic residuesSequence analysisAdd BLAST | 29 |
Domaini
The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.
Keywords - Domaini
Repeat, SignalPhylogenomic databases
eggNOGi | KOG3538, Eukaryota |
InParanoidi | P57110 |
PhylomeDBi | P57110 |
Family and domain databases
Gene3Di | 2.20.100.10, 2 hits 3.40.390.10, 1 hit |
InterProi | View protein in InterPro IPR006586, ADAM_Cys-rich IPR013273, ADAMTS/ADAMTS-like IPR041645, ADAMTS_CR_2 IPR045371, ADAMTS_CR_3 IPR010294, ADAMTS_spacer1 IPR024079, MetalloPept_cat_dom_sf IPR013277, Pept_M12B_ADAM-TS8 IPR001590, Peptidase_M12B IPR002870, Peptidase_M12B_N IPR000884, TSP1_rpt IPR036383, TSP1_rpt_sf |
Pfami | View protein in Pfam PF17771, ADAM_CR_2, 1 hit PF19236, ADAM_CR_3, 1 hit PF05986, ADAM_spacer1, 1 hit PF01562, Pep_M12B_propep, 1 hit PF01421, Reprolysin, 1 hit PF00090, TSP_1, 1 hit |
PRINTSi | PR01861, ADAMTS8 PR01857, ADAMTSFAMILY |
SMARTi | View protein in SMART SM00608, ACR, 1 hit SM00209, TSP1, 2 hits |
SUPFAMi | SSF82895, SSF82895, 2 hits |
PROSITEi | View protein in PROSITE PS50215, ADAM_MEPRO, 1 hit PS50092, TSP1, 2 hits PS00142, ZINC_PROTEASE, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
P57110-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLRDPTTTGW PPLLLLLLQL PPPPLVCGAP AGPGTGAQAS ELVVPTRLPG
60 70 80 90 100
SASELAFHLS AFGQGFVLRL APDASFLAPE FKIERLGGSS AAAGGEPGLR
110 120 130 140 150
GCFFSGTVNG ERESLAAMSC VAGWSGSFLL AGEEFTIQPQ GAGDSLDQPH
160 170 180 190 200
RLQRWGPGQR REDPGLAAAE VFPLPQGLEW EVEMGNGQGQ ERSDNEEDRK
210 220 230 240 250
QDKEGLLKET EDSRKVPPPF GSKTRSKRFV SEARFVETLL VADASMAAFY
260 270 280 290 300
GTDLQNHILT VMSMAARIYK HPSIRNSVNL VVVKVLIVEK ERWGPEVSDN
310 320 330 340 350
GGLTLRNFCS WQRRFNKPSD RHPEHYDTAI LFTRQNFCGK GEQCDTLGMA
360 370 380 390 400
DVGTICDPDK SCSVIKDEGL QAAYTLAHEL GHVLSMPHDD SKPCVRLFGP
410 420 430 440 450
MGKYHMMAPF FIHVNKTLPW SPCSAVYLTE LLDDGHGDCL LDAPTSVLPL
460 470 480 490 500
PTGLPGHSTL YELDQQCKQI FGPDFRHCPN TSVEDICVQL CARHRDSDEP
510 520 530 540 550
ICHTKNGSLL WADGTPCGPG HLCLDGSCVL KEDVENPKAV VDGDWGPWRP
560 570 580 590 600
WGQCSRTCGG GIQFSNRECD NPMPQNGGRF CLGERVKYQS CNTEECPPNG
610 620 630 640 650
KSFREQQCEK YNAYNHTDLD GNFLQWVPKY SGVSPRDRCK LFCRARGRSE
660 670 680 690 700
FKVFEAKVID GTLCGPDTLS ICVRGQCVKA GCDHVVNSPK KLDKCGVCGG
710 720 730 740 750
KGTACRKISG SFTPFSYGYN DIVTIPAGAT NIDVKQRSHP GVRNDGSYLA
760 770 780 790 800
LKTANGQYLL NGNLAISAIE QDILVKGTIL KYSGSMATLE RLQSFQALPE
810 820 830 840 850
PLTVQLLTVS GEVFPPKVRY TFFVPNDMDF SVQNSKERAT TNIIQSLPSA
860 870 880 890 900
EWVLGDWSEC PSTCRGSWQR RTVECRDPSG QASDTCDEAL KPEDAKPCGS
QPCPL
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF8VQ15 | F8VQ15_MOUSE | A disintegrin and metalloproteinase... | Adamts8 | 905 | Annotation score: | ||
F6U6K2 | F6U6K2_MOUSE | A disintegrin and metalloproteinase... | Adamts8 | 142 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF175282 mRNA Translation: AAF25805.1 |
CCDSi | CCDS22946.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF175282 mRNA Translation: AAF25805.1 |
CCDSi | CCDS22946.1 |
3D structure databases
SMRi | P57110 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000069644 |
Protein family/group databases
MEROPSi | M12.226 |
PTM databases
GlyGeni | P57110, 4 sites |
PhosphoSitePlusi | P57110 |
Proteomic databases
PaxDbi | P57110 |
PRIDEi | P57110 |
ProteomicsDBi | 273587 |
Protocols and materials databases
DNASUi | 30806 |
Organism-specific databases
MGIi | MGI:1353468, Adamts8 |
Phylogenomic databases
eggNOGi | KOG3538, Eukaryota |
InParanoidi | P57110 |
PhylomeDBi | P57110 |
Enzyme and pathway databases
Reactomei | R-MMU-5173214, O-glycosylation of TSR domain-containing proteins |
Miscellaneous databases
PROi | PR:P57110 |
RNActi | P57110, protein |
SOURCEi | Search... |
Family and domain databases
Gene3Di | 2.20.100.10, 2 hits 3.40.390.10, 1 hit |
InterProi | View protein in InterPro IPR006586, ADAM_Cys-rich IPR013273, ADAMTS/ADAMTS-like IPR041645, ADAMTS_CR_2 IPR045371, ADAMTS_CR_3 IPR010294, ADAMTS_spacer1 IPR024079, MetalloPept_cat_dom_sf IPR013277, Pept_M12B_ADAM-TS8 IPR001590, Peptidase_M12B IPR002870, Peptidase_M12B_N IPR000884, TSP1_rpt IPR036383, TSP1_rpt_sf |
Pfami | View protein in Pfam PF17771, ADAM_CR_2, 1 hit PF19236, ADAM_CR_3, 1 hit PF05986, ADAM_spacer1, 1 hit PF01562, Pep_M12B_propep, 1 hit PF01421, Reprolysin, 1 hit PF00090, TSP_1, 1 hit |
PRINTSi | PR01861, ADAMTS8 PR01857, ADAMTSFAMILY |
SMARTi | View protein in SMART SM00608, ACR, 1 hit SM00209, TSP1, 2 hits |
SUPFAMi | SSF82895, SSF82895, 2 hits |
PROSITEi | View protein in PROSITE PS50215, ADAM_MEPRO, 1 hit PS50092, TSP1, 2 hits PS00142, ZINC_PROTEASE, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ATS8_MOUSE | |
Accessioni | P57110Primary (citable) accession number: P57110 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 2000 |
Last sequence update: | December 1, 2000 | |
Last modified: | February 23, 2022 | |
This is version 152 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot