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Protein

A disintegrin and metalloproteinase with thrombospondin motifs 8

Gene

Adamts8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Has anti-angiogenic properties.By similarity

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi378Zinc; catalyticBy similarity1
Active sitei379PROSITE-ProRule annotation1
Metal bindingi382Zinc; catalyticBy similarity1
Metal bindingi388Zinc; catalyticBy similarity1

GO - Molecular functioni

Keywordsi

Molecular functionHeparin-binding, Hydrolase, Metalloprotease, Protease
LigandMetal-binding, Zinc

Protein family/group databases

MEROPSiM12.226

Names & Taxonomyi

Protein namesi
Recommended name:
A disintegrin and metalloproteinase with thrombospondin motifs 8 (EC:3.4.24.-)
Short name:
ADAM-TS 8
Short name:
ADAM-TS8
Short name:
ADAMTS-8
Alternative name(s):
METH-2
Gene namesi
Name:Adamts8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1353468 Adamts8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 28Sequence analysisAdd BLAST28
PropeptideiPRO_000002918029 – 228By similarityAdd BLAST200
ChainiPRO_0000029181229 – 905A disintegrin and metalloproteinase with thrombospondin motifs 8Add BLAST677

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi309 ↔ 362By similarity
Disulfide bondi338 ↔ 344By similarity
Disulfide bondi356 ↔ 439By similarity
Disulfide bondi394 ↔ 423By similarity
Glycosylationi415N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi478 ↔ 502By similarity
Glycosylationi480N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi487 ↔ 523By similarity
Glycosylationi506N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi517 ↔ 528By similarity
Disulfide bondi554 ↔ 591By similarity
Disulfide bondi558 ↔ 596By similarity
Disulfide bondi569 ↔ 581By similarity
Glycosylationi615N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

The precursor is cleaved by a furin endopeptidase.By similarity
Glycosylated. Can be O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X2-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS family members. Also can be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs, and N-glycosylated. These other glycosylations can also facilitate secretion (By similarity).By similarity

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiP57110
PRIDEiP57110

PTM databases

PhosphoSitePlusiP57110

Expressioni

Tissue specificityi

Expressed specifically in adult lung and heart and low expression during mouse development.

Structurei

3D structure databases

ProteinModelPortaliP57110
SMRiP57110
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini234 – 444Peptidase M12BPROSITE-ProRule annotationAdd BLAST211
Domaini453 – 541DisintegrinAdd BLAST89
Domaini542 – 597TSP type-1 1PROSITE-ProRule annotationAdd BLAST56
Domaini848 – 904TSP type-1 2PROSITE-ProRule annotationAdd BLAST57

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni706 – 847SpacerAdd BLAST142

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi599 – 705Cys-richAdd BLAST107

Domaini

The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiKOG3538 Eukaryota
ENOG410XPKZ LUCA
HOGENOMiHOG000004799
HOVERGENiHBG004313
InParanoidiP57110
PhylomeDBiP57110

Family and domain databases

Gene3Di2.20.100.10, 2 hits
3.40.390.10, 1 hit
InterProiView protein in InterPro
IPR006586 ADAM_Cys-rich
IPR010294 ADAM_spacer1
IPR024079 MetalloPept_cat_dom_sf
IPR013277 Pept_M12B_ADAM-TS8
IPR001590 Peptidase_M12B
IPR002870 Peptidase_M12B_N
IPR000884 TSP1_rpt
IPR036383 TSP1_rpt_sf
PfamiView protein in Pfam
PF05986 ADAM_spacer1, 1 hit
PF01562 Pep_M12B_propep, 1 hit
PF01421 Reprolysin, 1 hit
PF00090 TSP_1, 1 hit
PRINTSiPR01861 ADAMTS8
SMARTiView protein in SMART
SM00608 ACR, 1 hit
SM00209 TSP1, 2 hits
SUPFAMiSSF82895 SSF82895, 2 hits
PROSITEiView protein in PROSITE
PS50215 ADAM_MEPRO, 1 hit
PS50092 TSP1, 2 hits
PS00142 ZINC_PROTEASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P57110-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRDPTTTGW PPLLLLLLQL PPPPLVCGAP AGPGTGAQAS ELVVPTRLPG
60 70 80 90 100
SASELAFHLS AFGQGFVLRL APDASFLAPE FKIERLGGSS AAAGGEPGLR
110 120 130 140 150
GCFFSGTVNG ERESLAAMSC VAGWSGSFLL AGEEFTIQPQ GAGDSLDQPH
160 170 180 190 200
RLQRWGPGQR REDPGLAAAE VFPLPQGLEW EVEMGNGQGQ ERSDNEEDRK
210 220 230 240 250
QDKEGLLKET EDSRKVPPPF GSKTRSKRFV SEARFVETLL VADASMAAFY
260 270 280 290 300
GTDLQNHILT VMSMAARIYK HPSIRNSVNL VVVKVLIVEK ERWGPEVSDN
310 320 330 340 350
GGLTLRNFCS WQRRFNKPSD RHPEHYDTAI LFTRQNFCGK GEQCDTLGMA
360 370 380 390 400
DVGTICDPDK SCSVIKDEGL QAAYTLAHEL GHVLSMPHDD SKPCVRLFGP
410 420 430 440 450
MGKYHMMAPF FIHVNKTLPW SPCSAVYLTE LLDDGHGDCL LDAPTSVLPL
460 470 480 490 500
PTGLPGHSTL YELDQQCKQI FGPDFRHCPN TSVEDICVQL CARHRDSDEP
510 520 530 540 550
ICHTKNGSLL WADGTPCGPG HLCLDGSCVL KEDVENPKAV VDGDWGPWRP
560 570 580 590 600
WGQCSRTCGG GIQFSNRECD NPMPQNGGRF CLGERVKYQS CNTEECPPNG
610 620 630 640 650
KSFREQQCEK YNAYNHTDLD GNFLQWVPKY SGVSPRDRCK LFCRARGRSE
660 670 680 690 700
FKVFEAKVID GTLCGPDTLS ICVRGQCVKA GCDHVVNSPK KLDKCGVCGG
710 720 730 740 750
KGTACRKISG SFTPFSYGYN DIVTIPAGAT NIDVKQRSHP GVRNDGSYLA
760 770 780 790 800
LKTANGQYLL NGNLAISAIE QDILVKGTIL KYSGSMATLE RLQSFQALPE
810 820 830 840 850
PLTVQLLTVS GEVFPPKVRY TFFVPNDMDF SVQNSKERAT TNIIQSLPSA
860 870 880 890 900
EWVLGDWSEC PSTCRGSWQR RTVECRDPSG QASDTCDEAL KPEDAKPCGS

QPCPL
Length:905
Mass (Da):98,880
Last modified:December 1, 2000 - v1
Checksum:i124D4132B33A0CAE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF175282 mRNA Translation: AAF25805.1
CCDSiCCDS22946.1
UniGeneiMm.100582

Similar proteinsi

Entry informationi

Entry nameiATS8_MOUSE
AccessioniPrimary (citable) accession number: P57110
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: July 18, 2018
This is version 140 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

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