UniProtKB - P57094 (AXIN1_DANRE)
Protein
Axin-1
Gene
axin1
Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Functioni
Component of the beta-catenin destruction complex required for regulating ctnnb1 levels through phosphorylation and ubiquitination, and modulating Wnt-signaling (PubMed:17681137). Controls dorsoventral patterning via two opposing effects: down-regulates ctnnb1 to inhibit the Wnt signaling pathway and ventralize embryos, but also dorsalizes embryos by activating a Wnt-independent JNK signaling pathway (PubMed:17681137, PubMed:30467143).2 Publications
GO - Molecular functioni
- beta-catenin binding Source: InterPro
- protein homodimerization activity Source: UniProtKB
GO - Biological processi
- activation of JUN kinase activity Source: ZFIN
- determination of left/right symmetry Source: ZFIN
- dorsal/ventral axis specification Source: MGI
- dorsal/ventral pattern formation Source: ZFIN
- embryonic eye morphogenesis Source: ZFIN
- eye development Source: ZFIN
- fin regeneration Source: ZFIN
- forebrain anterior/posterior pattern specification Source: ZFIN
- forebrain development Source: ZFIN
- forebrain neuron fate commitment Source: ZFIN
- muscle cell development Source: ZFIN
- negative regulation of canonical Wnt signaling pathway Source: ZFIN
- negative regulation of Wnt signaling pathway Source: ZFIN
- negative regulation of Wnt signaling pathway involved in dorsal/ventral axis specification Source: UniProtKB
- neural plate anterior/posterior regionalization Source: ZFIN
- olfactory placode formation Source: ZFIN
- optic placode formation Source: ZFIN
- positive regulation of JNK cascade Source: UniProtKB
- positive regulation of JUN kinase activity Source: MGI
- somitogenesis Source: ZFIN
- Wnt signaling pathway involved in somitogenesis Source: ZFIN
Keywordsi
Molecular function | Developmental protein |
Biological process | Wnt signaling pathway |
Enzyme and pathway databases
Reactomei | R-DRE-195253, Degradation of beta-catenin by the destruction complex R-DRE-196299, Beta-catenin phosphorylation cascade R-DRE-201681, TCF dependent signaling in response to WNT R-DRE-4641257, Degradation of AXIN R-DRE-4641262, Disassembly of the destruction complex and recruitment of AXIN to the membrane R-DRE-5689880, Ub-specific processing proteases |
Names & Taxonomyi
Protein namesi | Recommended name: Axin-1Alternative name(s): Axis inhibition protein 1 |
Gene namesi | Name:axin1 Synonyms:axin |
Organismi | Danio rerio (Zebrafish) (Brachydanio rerio) |
Taxonomic identifieri | 7955 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Danionidae › Danioninae › Danio |
Proteomesi |
|
Organism-specific databases
ZFINi | ZDB-GENE-000403-1, axin1 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity
Nucleus
- Nucleus By similarity
Other locations
Nucleus
- nucleus Source: UniProtKB-SubCell
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- beta-catenin destruction complex Source: ZFIN
Keywords - Cellular componenti
Cell membrane, Cytoplasm, Membrane, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000220893 | 1 – 835 | Axin-1Add BLAST | 835 |
Post-translational modificationi
ADP-ribosylated by tankyrase tnks and tnks2. Poly-ADP-ribosylated protein is recognized by rnf146, followed by ubiquitination at 'Lys-48' and subsequent activation of the Wnt signaling pathway.1 Publication
Ubiquitinated by rnf146 when poly-ADP-ribosylated, leading to its degradation and subsequent activation of the Wnt signaling pathway.1 Publication
Keywords - PTMi
ADP-ribosylation, Phosphoprotein, Ubl conjugationProteomic databases
PaxDbi | P57094 |
Interactioni
Subunit structurei
GO - Molecular functioni
- beta-catenin binding Source: InterPro
- protein homodimerization activity Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 78941, 3 interactors |
ELMi | P57094 |
STRINGi | 7955.ENSDARP00000036978 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 92 – 214 | RGSPROSITE-ProRule annotationAdd BLAST | 123 | |
Domaini | 753 – 835 | DIXPROSITE-ProRule annotationAdd BLAST | 83 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 351 – 436 | Interaction with GSK3BBy similarityAdd BLAST | 86 | |
Regioni | 437 – 512 | Interaction with beta-cateninBy similarityAdd BLAST | 76 |
Domaini
The DIX domain mediates interaction with dixdc1.1 Publication
Phylogenomic databases
eggNOGi | KOG3589, Eukaryota |
InParanoidi | P57094 |
PhylomeDBi | P57094 |
Family and domain databases
CDDi | cd11582, Axin_TNKS_binding, 1 hit |
Gene3Di | 1.10.196.10, 2 hits 3.10.20.380, 1 hit |
InterProi | View protein in InterPro IPR043581, Axin-like IPR029797, AXIN1 IPR014936, Axin_b-cat-bd IPR032101, Axin_TNKS-bd IPR001158, DIX IPR038207, DIX_dom_sf IPR016137, RGS IPR036305, RGS_sf IPR024066, RGS_subdom1/3 IPR029071, Ubiquitin-like_domsf |
PANTHERi | PTHR46102, PTHR46102, 1 hit PTHR46102:SF3, PTHR46102:SF3, 1 hit |
Pfami | View protein in Pfam PF16646, AXIN1_TNKS_BD, 1 hit PF08833, Axin_b-cat_bind, 1 hit PF00778, DIX, 1 hit PF00615, RGS, 1 hit |
PRINTSi | PR01301, RGSPROTEIN |
SMARTi | View protein in SMART SM00021, DAX, 1 hit SM00315, RGS, 1 hit |
SUPFAMi | SSF48097, SSF48097, 1 hit SSF54236, SSF54236, 1 hit |
PROSITEi | View protein in PROSITE PS50841, DIX, 1 hit PS50132, RGS, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
P57094-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSMSVNEKGI CYLPDLGSSF TEDAPRPPVP GEEGDLVSSD GRQYNHSFYS
60 70 80 90 100
SKSDSLKNEA SIATPRRPDL DLGYEPEGSA SPTPPYLKWA ESLHSLLDDQ
110 120 130 140 150
DGIHLFRTFL KQEECADMLD FWFACSGFRK QEANDGNEKM LKLAKAIYKK
160 170 180 190 200
YILDNNGIVS RQIKPATKSF IKDCVTKLHI DPAMFDQAQT EIQTMMEENT
210 220 230 240 250
YPLFLKSDIY LEYTRTGGES PKLFSDQSSV SGNGKVLPGY LPTVIEDVEW
260 270 280 290 300
RCDQEEEQIA ESDPTPSNRL TQKLPLETVP QRVANSKRYQ DNREYRHASW
310 320 330 340 350
REPVNPYYVN SGYALAPATS ANDSEQQSMS SDADTLSLTD SSVDGVPPYR
360 370 380 390 400
YRKPHRREIH ESAKVNGRVP LPHIPRTNRI PKDIHVEPEK FAAELISRLE
410 420 430 440 450
GVLREREAQE KLEERLKRVR LEEEGDDADI STGPSLANHR VPPAVHVQHY
460 470 480 490 500
GGRYSEMSYN GLQLRDAHEE NPESILDEHV QRVMKTPGCQ SPGTGRHSPK
510 520 530 540 550
SRSPDGLPAG KIPGLMMPLS GGQGKHQARQ GPKGEAAHLH HHKHIHHTHY
560 570 580 590 600
AAAGKPKEQA EAEAARMHGG FAWNTEQHHY GPKSRNYADG MSVGPNTMDP
610 620 630 640 650
MGYSSKGSTL SKRPVRKGED GRNFEMREPL PADDMERNQK ILQWMMEGEK
660 670 680 690 700
EAGRYKRGPY GSISGPKKAQ GHEPARPSSV ERLGAVHPWV TAQLRNNVQP
710 720 730 740 750
SHPFIQDPTM PPNPAPNPLT QLEEARRRLE EERRKSGTLQ AKQRHKNMKK
760 770 780 790 800
QPCENITVAY YFRGEPIPYR TSVKGRIVTL GQFKELLTKK GSYKYYFKKV
810 820 830
SYEFDCGVVF EEVREDDAIL PIFEEKIIGK VEKVD
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0R4II61 | A0A0R4II61_DANRE | Axin-1 | axin1 | 835 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 176 | T → M in BAA92439 (PubMed:10704853).Curated | 1 | |
Sequence conflicti | 275 | P → L in BAA92439 (PubMed:10704853).Curated | 1 | |
Sequence conflicti | 658 | G → S in BAA92439 (PubMed:10704853).Curated | 1 | |
Sequence conflicti | 763 | R → C in BAA92439 (PubMed:10704853).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB032262 mRNA Translation: BAA92439.1 BC099991 mRNA Translation: AAH99991.1 |
RefSeqi | NP_571578.2, NM_131503.2 |
Genome annotation databases
GeneIDi | 57931 |
KEGGi | dre:57931 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB032262 mRNA Translation: BAA92439.1 BC099991 mRNA Translation: AAH99991.1 |
RefSeqi | NP_571578.2, NM_131503.2 |
3D structure databases
SMRi | P57094 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 78941, 3 interactors |
ELMi | P57094 |
STRINGi | 7955.ENSDARP00000036978 |
Proteomic databases
PaxDbi | P57094 |
Genome annotation databases
GeneIDi | 57931 |
KEGGi | dre:57931 |
Organism-specific databases
CTDi | 8312 |
ZFINi | ZDB-GENE-000403-1, axin1 |
Phylogenomic databases
eggNOGi | KOG3589, Eukaryota |
InParanoidi | P57094 |
PhylomeDBi | P57094 |
Enzyme and pathway databases
Reactomei | R-DRE-195253, Degradation of beta-catenin by the destruction complex R-DRE-196299, Beta-catenin phosphorylation cascade R-DRE-201681, TCF dependent signaling in response to WNT R-DRE-4641257, Degradation of AXIN R-DRE-4641262, Disassembly of the destruction complex and recruitment of AXIN to the membrane R-DRE-5689880, Ub-specific processing proteases |
Miscellaneous databases
PROi | PR:P57094 |
Family and domain databases
CDDi | cd11582, Axin_TNKS_binding, 1 hit |
Gene3Di | 1.10.196.10, 2 hits 3.10.20.380, 1 hit |
InterProi | View protein in InterPro IPR043581, Axin-like IPR029797, AXIN1 IPR014936, Axin_b-cat-bd IPR032101, Axin_TNKS-bd IPR001158, DIX IPR038207, DIX_dom_sf IPR016137, RGS IPR036305, RGS_sf IPR024066, RGS_subdom1/3 IPR029071, Ubiquitin-like_domsf |
PANTHERi | PTHR46102, PTHR46102, 1 hit PTHR46102:SF3, PTHR46102:SF3, 1 hit |
Pfami | View protein in Pfam PF16646, AXIN1_TNKS_BD, 1 hit PF08833, Axin_b-cat_bind, 1 hit PF00778, DIX, 1 hit PF00615, RGS, 1 hit |
PRINTSi | PR01301, RGSPROTEIN |
SMARTi | View protein in SMART SM00021, DAX, 1 hit SM00315, RGS, 1 hit |
SUPFAMi | SSF48097, SSF48097, 1 hit SSF54236, SSF54236, 1 hit |
PROSITEi | View protein in PROSITE PS50841, DIX, 1 hit PS50132, RGS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AXIN1_DANRE | |
Accessioni | P57094Primary (citable) accession number: P57094 Secondary accession number(s): Q499B7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 2000 |
Last sequence update: | July 22, 2008 | |
Last modified: | December 2, 2020 | |
This is version 144 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |