UniProtKB - P56965 (DDAH1_BOVIN)
Protein
N(G),N(G)-dimethylarginine dimethylaminohydrolase 1
Gene
DDAH1
Organism
Bos taurus (Bovine)
Status
Functioni
Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation.
Caution
Was originally thought to be a heme protein, but this was later shown to be an artifact.1 Publication
Catalytic activityi
- EC:3.5.3.181 Publication
Activity regulationi
Copurifies with a tightly bound zinc ion. Activated by release of zinc. His and other agents that promote the release of bound zinc ions activate the enzyme (in vitro). Inhibited by S-nitrosylation. Zinc protects the protein against S-nitrosylation.3 Publications
pH dependencei
Optimum pH is 7.8.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 30 | Substrate; via carbonyl oxygen | 1 | |
Binding sitei | 73 | Substrate | 1 | |
Binding sitei | 78 | Substrate | 1 | |
Binding sitei | 79 | Substrate | 1 | |
Binding sitei | 98 | Substrate | 1 | |
Binding sitei | 145 | Substrate | 1 | |
Active sitei | 173 | Proton donorBy similarity | 1 | |
Binding sitei | 268 | Substrate; via carbonyl oxygen | 1 | |
Active sitei | 274 | NucleophileBy similarity | 1 | |
Metal bindingi | 274 | Zinc | 1 |
GO - Molecular functioni
- amino acid binding Source: GO_Central
- dimethylargininase activity Source: MGI
- hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amidines Source: GO_Central
- zinc ion binding Source: MGI
GO - Biological processi
- arginine catabolic process Source: Ensembl
- arginine metabolic process Source: GO_Central
- citrulline metabolic process Source: UniProtKB
- negative regulation of cell population proliferation Source: Ensembl
- negative regulation of cellular response to hypoxia Source: Ensembl
- negative regulation of vascular permeability Source: Ensembl
- nitric oxide biosynthetic process Source: MGI
- positive regulation of nitric oxide biosynthetic process Source: BHF-UCL
- protein nitrosylation Source: MGI
- regulation of systemic arterial blood pressure Source: Ensembl
Keywordsi
Molecular function | Hydrolase |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
BRENDAi | 3.5.3.18, 908 |
Reactomei | R-BTA-203615, eNOS activation |
SABIO-RKi | P56965 |
Names & Taxonomyi
Protein namesi | Recommended name: N(G),N(G)-dimethylarginine dimethylaminohydrolase 1 (EC:3.5.3.18)Short name: DDAH-1 Short name: Dimethylarginine dimethylaminohydrolase 1 Alternative name(s): DDAHI Dimethylargininase-1 |
Gene namesi | Name:DDAH1 |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
Organism-specific databases
VGNCi | VGNC:27939, DDAH1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed2 Publications | |||
ChainiPRO_0000171117 | 2 – 285 | N(G),N(G)-dimethylarginine dimethylaminohydrolase 1Add BLAST | 284 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanine1 Publication | 1 | |
Modified residuei | 222 | S-nitrosocysteine1 Publication | 1 | |
Modified residuei | 274 | S-nitrosocysteine1 Publication | 1 |
Keywords - PTMi
Acetylation, S-nitrosylationProteomic databases
PaxDbi | P56965 |
PeptideAtlasi | P56965 |
PRIDEi | P56965 |
PTM databases
iPTMneti | P56965 |
Expressioni
Tissue specificityi
Widely distributed, highest concentrations found in brain, brain cortex and kidney (at protein level).1 Publication
Gene expression databases
Bgeei | ENSBTAG00000034776, Expressed in prefrontal cortex and 17 other tissues |
Interactioni
Subunit structurei
Monomer.
2 PublicationsProtein-protein interaction databases
BioGRIDi | 193961, 1 interactor |
STRINGi | 9913.ENSBTAP00000046157 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P56965 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P56965 |
Family & Domainsi
Sequence similaritiesi
Belongs to the DDAH family.Curated
Phylogenomic databases
eggNOGi | ENOG502QWPA, Eukaryota |
GeneTreei | ENSGT00940000157892 |
HOGENOMi | CLU_067923_0_0_1 |
InParanoidi | P56965 |
OMAi | GPDVICV |
OrthoDBi | 1469762at2759 |
TreeFami | TF314737 |
Family and domain databases
InterProi | View protein in InterPro IPR033199, DDAH IPR033197, DDAH1 |
PANTHERi | PTHR12737, PTHR12737, 1 hit PTHR12737:SF17, PTHR12737:SF17, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P56965-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MASLGHPATF GRATHVVVRA LPESLAQQAL RRTKGDEVDF ARAERQHQLY
60 70 80 90 100
VGVLGSKLGL QVVQLPADES LPDCVFVEDV AVVCEETALI TRPGAPSRRK
110 120 130 140 150
EADMMKEALE KLQLNIVEMK DENATLDGGD VLFTGREFFV GLSKRTNQRG
160 170 180 190 200
AEILADTFKD YAVSTVPVVD ALHLKSFCSM AGPNLIAIGS SESAQKALKI
210 220 230 240 250
MQQMSDHRYD KLTVPDDTAA NCIYLNIPSK GHVLLHRTPE EYPESAKVYE
260 270 280
KLKDHMLIPV SNSELEKVDG LLTCSSVLIN KKVDS
Mass spectrometryi
Molecular mass is 31199 Da. Determined by ESI. 1 Publication
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC149998 mRNA Translation: AAI49999.1 |
PIRi | S74156 |
RefSeqi | NP_001095671.1, NM_001102201.2 |
Genome annotation databases
Ensembli | ENSBTAT00000049235; ENSBTAP00000046157; ENSBTAG00000034776 |
GeneIDi | 537391 |
KEGGi | bta:537391 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC149998 mRNA Translation: AAI49999.1 |
PIRi | S74156 |
RefSeqi | NP_001095671.1, NM_001102201.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2C6Z | X-ray | 1.20 | A | 2-285 | [»] | |
2CI1 | X-ray | 1.08 | A | 8-282 | [»] | |
2CI3 | X-ray | 1.70 | A | 2-285 | [»] | |
2CI4 | X-ray | 1.70 | A | 2-285 | [»] | |
2CI5 | X-ray | 1.79 | A/B | 2-285 | [»] | |
2CI6 | X-ray | 2.00 | A | 2-285 | [»] | |
2CI7 | X-ray | 1.60 | A | 2-285 | [»] | |
SMRi | P56965 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 193961, 1 interactor |
STRINGi | 9913.ENSBTAP00000046157 |
Chemistry databases
ChEMBLi | CHEMBL6081 |
PTM databases
iPTMneti | P56965 |
Proteomic databases
PaxDbi | P56965 |
PeptideAtlasi | P56965 |
PRIDEi | P56965 |
Genome annotation databases
Ensembli | ENSBTAT00000049235; ENSBTAP00000046157; ENSBTAG00000034776 |
GeneIDi | 537391 |
KEGGi | bta:537391 |
Organism-specific databases
CTDi | 23576 |
VGNCi | VGNC:27939, DDAH1 |
Phylogenomic databases
eggNOGi | ENOG502QWPA, Eukaryota |
GeneTreei | ENSGT00940000157892 |
HOGENOMi | CLU_067923_0_0_1 |
InParanoidi | P56965 |
OMAi | GPDVICV |
OrthoDBi | 1469762at2759 |
TreeFami | TF314737 |
Enzyme and pathway databases
BRENDAi | 3.5.3.18, 908 |
Reactomei | R-BTA-203615, eNOS activation |
SABIO-RKi | P56965 |
Miscellaneous databases
EvolutionaryTracei | P56965 |
PROi | PR:P56965 |
Gene expression databases
Bgeei | ENSBTAG00000034776, Expressed in prefrontal cortex and 17 other tissues |
Family and domain databases
InterProi | View protein in InterPro IPR033199, DDAH IPR033197, DDAH1 |
PANTHERi | PTHR12737, PTHR12737, 1 hit PTHR12737:SF17, PTHR12737:SF17, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DDAH1_BOVIN | |
Accessioni | P56965Primary (citable) accession number: P56965 Secondary accession number(s): A6QQU7, P81020 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 2000 |
Last sequence update: | January 23, 2007 | |
Last modified: | December 2, 2020 | |
This is version 131 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families