UniProtKB - P56914 (NUOG2_RHIME)
Protein
NADH-quinone oxidoreductase subunit G 2
Gene
nuoG2
Organism
Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
Status
Functioni
NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient (By similarity).By similarity
Catalytic activityi
Cofactori
Protein has several cofactor binding sites:- [2Fe-2S] clusterBy similarityNote: Binds 1 [2Fe-2S] cluster per subunit.By similarity
- [4Fe-4S] clusterBy similarityNote: Binds 3 [4Fe-4S] clusters per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 34 | Iron-sulfur 1 (2Fe-2S)By similarity | 1 | |
Metal bindingi | 45 | Iron-sulfur 1 (2Fe-2S)By similarity | 1 | |
Metal bindingi | 48 | Iron-sulfur 1 (2Fe-2S)By similarity | 1 | |
Metal bindingi | 62 | Iron-sulfur 1 (2Fe-2S)By similarity | 1 | |
Metal bindingi | 94 | Iron-sulfur 2 (4Fe-4S); via tele nitrogenPROSITE-ProRule annotation | 1 | |
Metal bindingi | 98 | Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation | 1 | |
Metal bindingi | 101 | Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation | 1 | |
Metal bindingi | 107 | Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation | 1 | |
Metal bindingi | 148 | Iron-sulfur 3 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 151 | Iron-sulfur 3 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 154 | Iron-sulfur 3 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 198 | Iron-sulfur 3 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 224 | Iron-sulfur 4 (4Fe-4S)Sequence analysis | 1 | |
Metal bindingi | 227 | Iron-sulfur 4 (4Fe-4S)Sequence analysis | 1 | |
Metal bindingi | 231 | Iron-sulfur 4 (4Fe-4S)Sequence analysis | 1 | |
Metal bindingi | 259 | Iron-sulfur 4 (4Fe-4S)Sequence analysis | 1 |
GO - Molecular functioni
- 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
- 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
- molybdopterin cofactor binding Source: InterPro
- NADH dehydrogenase (ubiquinone) activity Source: InterPro
- quinone binding Source: UniProtKB-KW
GO - Biological processi
- ATP synthesis coupled electron transport Source: InterPro
Keywordsi
Molecular function | Translocase |
Ligand | 2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone |
Names & Taxonomyi
Protein namesi | Recommended name: NADH-quinone oxidoreductase subunit G 2 (EC:7.1.1.-)Alternative name(s): NADH dehydrogenase I subunit G 2 NDH-1 subunit G 2 |
Gene namesi | Name:nuoG2 Ordered Locus Names:RA0828 ORF Names:SMa1523 |
Encoded oni | Plasmid pSymA (megaplasmid 1) |
Organismi | Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti) |
Taxonomic identifieri | 266834 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000118560 | 1 – 853 | NADH-quinone oxidoreductase subunit G 2Add BLAST | 853 |
Keywords - PTMi
QuinoneFamily & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 78 | 2Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd BLAST | 78 | |
Domaini | 78 – 117 | 4Fe-4S His(Cys)3-ligated-typePROSITE-ProRule annotationAdd BLAST | 40 | |
Domaini | 139 – 170 | 4Fe-4S ferredoxin-type 1PROSITE-ProRule annotationAdd BLAST | 32 | |
Domaini | 179 – 209 | 4Fe-4S ferredoxin-type 2PROSITE-ProRule annotationAdd BLAST | 31 | |
Domaini | 217 – 273 | 4Fe-4S Mo/W bis-MGD-typePROSITE-ProRule annotationAdd BLAST | 57 |
Sequence similaritiesi
Belongs to the complex I 75 kDa subunit family.Curated
Keywords - Domaini
RepeatPhylogenomic databases
HOGENOMi | CLU_000422_11_6_5 |
OMAi | PSICHGC |
Family and domain databases
CDDi | cd00207, fer2, 1 hit |
InterProi | View protein in InterPro IPR036010, 2Fe-2S_ferredoxin-like_sf IPR001041, 2Fe-2S_ferredoxin-type IPR017896, 4Fe4S_Fe-S-bd IPR017900, 4Fe4S_Fe_S_CS IPR009010, Asp_de-COase-like_dom_sf IPR006657, MoPterin_dinucl-bd_dom IPR006656, Mopterin_OxRdtase IPR006963, Mopterin_OxRdtase_4Fe-4S_dom IPR027467, MopterinOxRdtase_cofactor_BS IPR000283, NADH_UbQ_OxRdtase_75kDa_su_CS IPR010228, NADH_UbQ_OxRdtase_Gsu IPR019574, NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd |
Pfami | View protein in Pfam PF12838, Fer4_7, 1 hit PF04879, Molybdop_Fe4S4, 1 hit PF00384, Molybdopterin, 1 hit PF01568, Molydop_binding, 1 hit PF10588, NADH-G_4Fe-4S_3, 1 hit |
SMARTi | View protein in SMART SM00926, Molybdop_Fe4S4, 1 hit SM00929, NADH-G_4Fe-4S_3, 1 hit |
SUPFAMi | SSF50692, SSF50692, 1 hit SSF54292, SSF54292, 1 hit |
TIGRFAMsi | TIGR01973, NuoG, 1 hit |
PROSITEi | View protein in PROSITE PS51085, 2FE2S_FER_2, 1 hit PS00198, 4FE4S_FER_1, 2 hits PS51379, 4FE4S_FER_2, 2 hits PS51839, 4FE4S_HC3, 1 hit PS51669, 4FE4S_MOW_BIS_MGD, 1 hit PS00641, COMPLEX1_75K_1, 1 hit PS00642, COMPLEX1_75K_2, 1 hit PS00643, COMPLEX1_75K_3, 1 hit |
i Sequence
Sequence statusi: Complete.
P56914-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MIKVTIDEQS LEVEAGSTVL AAAERLGIEI PTFCYWKRLP PLASCRMCLV
60 70 80 90 100
EIEGLRRLQP ACATVAADGM VVRTNTPLIE ETRSSMLDML LANHPLDCPI
110 120 130 140 150
CDKGGECELQ DMVMAYGPGE SRFRDPKRVF HSKDIRLSPV IIMNVNRCIQ
160 170 180 190 200
CQRCVRMCEE VVGAVALGTV EKGMDTAVTG FEGSLASCDQ CGNCVEVCPV
210 220 230 240 250
GALMSFPYRY KARPWDLAET DTICPHCGTG CQLTVGARKG EFMRVRSDWE
260 270 280 290 300
HGVNRETLCV RGRFGLDFIE SRDRIKRPMI RRDGTLTPVS WEEAGDFLRQ
310 320 330 340 350
RLGVAEGKAA GGLISPRLPN EVLYQFQKLM RTVLRTNNVD CSSRWSAPLD
360 370 380 390 400
ILVPIVASFY SRDPLEQVIG KDCVLIIGGN VTEENPVTEY LLRDAARRRH
410 420 430 440 450
TRLLMLSARP SRLDADARAV LRAHPGGEGQ SLAAVVAALV AVTDEGLPDD
460 470 480 490 500
IFAKTSGTTA SSGANDALDR LVSTLKEGRS VTLLVSVDLL RSPLARKTLE
510 520 530 540 550
QLGNLLQLLR LLGKEPSLQF LFDRANQMGA WDMGVLPGVL PGLSPIADEA
560 570 580 590 600
TRTRFERSWG AEIPREPGAD VDAMLELCEK GGMGVLYVVG SDPLISYPDR
610 620 630 640 650
EFVERALGAA NLLIVQDAFL TDTAGLADVV LPAAGYGEES GTFTNNEGRT
660 670 680 690 700
QALRKFREPA FDARSNLAIF GFIAALRERP LQPSTETVIF EEMTRLVPAY
710 720 730 740 750
EGLTWEGLGA DGAFTTSAPK PWTSGFFAPL SAPAVTDVLQ LITGNCLFHN
760 770 780 790 800
GYVSEHSETL NSVADDPFIE MSAQDAAGLS LSDGDQVLVR SARGELTAKL
810 820 830 840 850
KVNRRFPHGL VFVPENYRAL RLNSLMRRGE YPCPVEIREC AKRAASALDE
ERV
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 211 | K → R in CAB51635 (Ref. 1) Curated | 1 | |
Sequence conflicti | 285 | T → A in CAB51635 (Ref. 1) Curated | 1 | |
Sequence conflicti | 452 | F → L in CAB51635 (Ref. 1) Curated | 1 | |
Sequence conflicti | 459 | T → I in CAB51635 (Ref. 1) Curated | 1 | |
Sequence conflicti | 544 | S → G in CAB51635 (Ref. 1) Curated | 1 | |
Sequence conflicti | 554 | R → K in CAB51635 (Ref. 1) Curated | 1 | |
Sequence conflicti | 679 | R → Q in CAB51635 (Ref. 1) Curated | 1 | |
Sequence conflicti | 734 | A → G in CAB51635 (Ref. 1) Curated | 1 | |
Sequence conflicti | 756 | H → R in CAB51635 (Ref. 1) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ245399 Genomic DNA Translation: CAB51635.1 AE006469 Genomic DNA Translation: AAK65486.1 |
PIRi | D95365 |
RefSeqi | NP_436074.1, NC_003037.1 WP_010967796.1, NC_003037.1 |
Genome annotation databases
EnsemblBacteriai | AAK65486; AAK65486; SMa1523 |
KEGGi | sme:SMa1523 |
PATRICi | fig|266834.11.peg.859 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ245399 Genomic DNA Translation: CAB51635.1 AE006469 Genomic DNA Translation: AAK65486.1 |
PIRi | D95365 |
RefSeqi | NP_436074.1, NC_003037.1 WP_010967796.1, NC_003037.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Genome annotation databases
EnsemblBacteriai | AAK65486; AAK65486; SMa1523 |
KEGGi | sme:SMa1523 |
PATRICi | fig|266834.11.peg.859 |
Phylogenomic databases
HOGENOMi | CLU_000422_11_6_5 |
OMAi | PSICHGC |
Family and domain databases
CDDi | cd00207, fer2, 1 hit |
InterProi | View protein in InterPro IPR036010, 2Fe-2S_ferredoxin-like_sf IPR001041, 2Fe-2S_ferredoxin-type IPR017896, 4Fe4S_Fe-S-bd IPR017900, 4Fe4S_Fe_S_CS IPR009010, Asp_de-COase-like_dom_sf IPR006657, MoPterin_dinucl-bd_dom IPR006656, Mopterin_OxRdtase IPR006963, Mopterin_OxRdtase_4Fe-4S_dom IPR027467, MopterinOxRdtase_cofactor_BS IPR000283, NADH_UbQ_OxRdtase_75kDa_su_CS IPR010228, NADH_UbQ_OxRdtase_Gsu IPR019574, NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd |
Pfami | View protein in Pfam PF12838, Fer4_7, 1 hit PF04879, Molybdop_Fe4S4, 1 hit PF00384, Molybdopterin, 1 hit PF01568, Molydop_binding, 1 hit PF10588, NADH-G_4Fe-4S_3, 1 hit |
SMARTi | View protein in SMART SM00926, Molybdop_Fe4S4, 1 hit SM00929, NADH-G_4Fe-4S_3, 1 hit |
SUPFAMi | SSF50692, SSF50692, 1 hit SSF54292, SSF54292, 1 hit |
TIGRFAMsi | TIGR01973, NuoG, 1 hit |
PROSITEi | View protein in PROSITE PS51085, 2FE2S_FER_2, 1 hit PS00198, 4FE4S_FER_1, 2 hits PS51379, 4FE4S_FER_2, 2 hits PS51839, 4FE4S_HC3, 1 hit PS51669, 4FE4S_MOW_BIS_MGD, 1 hit PS00641, COMPLEX1_75K_1, 1 hit PS00642, COMPLEX1_75K_2, 1 hit PS00643, COMPLEX1_75K_3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NUOG2_RHIME | |
Accessioni | P56914Primary (citable) accession number: P56914 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | November 2, 2001 | |
Last modified: | April 7, 2021 | |
This is version 135 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Plasmid, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families