nuoG2

Functionⁱ

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient (By similarity).By similarity

Catalytic activityⁱ

NADH + a quinone = NAD⁺ + a quinol.

Cofactorⁱ

Protein has several cofactor binding sites:

[2Fe-2S] clusterBy similarityNote: Binds 1 [2Fe-2S] cluster per subunit.By similarity
[4Fe-4S] clusterBy similarityNote: Binds 3 [4Fe-4S] clusters per subunit.By similarity

Sites

Feature key	Position(s)	DescriptionActions	Length
Metal bindingⁱ	34	Iron-sulfur 1 (2Fe-2S)By similarity	1
Metal bindingⁱ	45	Iron-sulfur 1 (2Fe-2S)By similarity	1
Metal bindingⁱ	48	Iron-sulfur 1 (2Fe-2S)By similarity	1
Metal bindingⁱ	62	Iron-sulfur 1 (2Fe-2S)By similarity	1
Metal bindingⁱ	94	Iron-sulfur 2 (4Fe-4S); via tele nitrogenPROSITE-ProRule annotation	1
Metal bindingⁱ	98	Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation	1
Metal bindingⁱ	101	Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation	1
Metal bindingⁱ	107	Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation	1
Metal bindingⁱ	148	Iron-sulfur 3 (4Fe-4S)By similarity	1
Metal bindingⁱ	151	Iron-sulfur 3 (4Fe-4S)By similarity	1
Metal bindingⁱ	154	Iron-sulfur 3 (4Fe-4S)By similarity	1
Metal bindingⁱ	198	Iron-sulfur 3 (4Fe-4S)By similarity	1
Metal bindingⁱ	224	Iron-sulfur 4 (4Fe-4S)Sequence analysis	1
Metal bindingⁱ	227	Iron-sulfur 4 (4Fe-4S)Sequence analysis	1
Metal bindingⁱ	231	Iron-sulfur 4 (4Fe-4S)Sequence analysis	1
Metal bindingⁱ	259	Iron-sulfur 4 (4Fe-4S)Sequence analysis	1

GO - Molecular functionⁱ

2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
electron transfer activity Source: InterPro
metal ion binding Source: UniProtKB-KW
molybdopterin cofactor binding Source: InterPro
NADH dehydrogenase (ubiquinone) activity Source: InterPro
quinone binding Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

ATP synthesis coupled electron transport Source: InterPro

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Oxidoreductase
Ligand	2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: NADH-quinone oxidoreductase subunit G 2 (EC:1.6.5.11) Alternative name(s): NADH dehydrogenase I subunit G 2 NDH-1 subunit G 2
Gene namesⁱ	Name:nuoG2 Ordered Locus Names:RA0828 ORF Names:SMa1523
Encoded onⁱ	Plasmid pSymA (megaplasmid 1)0 Publication
Organismⁱ	Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
Taxonomic identifierⁱ	266834 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium › Rhizobium meliloti
Proteomesⁱ	UP000001976 Componentⁱ: Plasmid pSymA

Subcellular locationⁱ

GO - Cellular componentⁱ

membrane Source: InterPro

View the complete GO annotation on QuickGO ...

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000118560	1 – 853	NADH-quinone oxidoreductase subunit G 2Add BLAST		853

Keywords - PTMⁱ

Quinone

Proteomic databases

PRIDEⁱ	P56914

PRIDEⁱ

P56914

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P56914
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Length
Domainⁱ	1 – 78	2Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd BLAST	78
Domainⁱ	78 – 117	4Fe-4S His(Cys)3-ligated-typePROSITE-ProRule annotationAdd BLAST	40
Domainⁱ	139 – 170	4Fe-4S ferredoxin-type 1PROSITE-ProRule annotationAdd BLAST	32
Domainⁱ	179 – 209	4Fe-4S ferredoxin-type 2PROSITE-ProRule annotationAdd BLAST	31
Domainⁱ	217 – 273	4Fe-4S Mo/W bis-MGD-typePROSITE-ProRule annotationAdd BLAST	57

Sequence similaritiesⁱ

Belongs to the complex I 75 kDa subunit family.Curated

Keywords - Domainⁱ

Repeat

Phylogenomic databases

HOGENOMⁱ	HOG000031440
KEGG Orthology (KO) More...KOⁱ	K00336
OMAⁱ	GYGYVNR

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00207 fer2, 1 hit
InterProⁱ	View protein in InterPro IPR036010 2Fe-2S_ferredoxin-like_sf IPR001041 2Fe-2S_ferredoxin-type IPR017896 4Fe4S_Fe-S-bd IPR017900 4Fe4S_Fe_S_CS IPR009010 Asp_de-COase-like_dom_sf IPR006657 MoPterin_dinucl-bd_dom IPR006656 Mopterin_OxRdtase IPR006963 Mopterin_OxRdtase_4Fe-4S_dom IPR027467 MopterinOxRdtase_cofactor_BS IPR000283 NADH_UbQ_OxRdtase_75kDa_su_CS IPR010228 NADH_UbQ_OxRdtase_Gsu IPR019574 NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF12838 Fer4_7, 1 hit PF04879 Molybdop_Fe4S4, 1 hit PF00384 Molybdopterin, 1 hit PF01568 Molydop_binding, 1 hit PF10588 NADH-G_4Fe-4S_3, 1 hit
SMARTⁱ	View protein in SMART SM00926 Molybdop_Fe4S4, 1 hit SM00929 NADH-G_4Fe-4S_3, 1 hit
SUPFAMⁱ	SSF50692 SSF50692, 1 hit SSF54292 SSF54292, 1 hit
TIGRFAMsⁱ	TIGR01973 NuoG, 1 hit
PROSITEⁱ	View protein in PROSITE PS51085 2FE2S_FER_2, 1 hit PS00198 4FE4S_FER_1, 2 hits PS51379 4FE4S_FER_2, 2 hits PS51839 4FE4S_HC3, 1 hit PS51669 4FE4S_MOW_BIS_MGD, 1 hit PS00641 COMPLEX1_75K_1, 1 hit PS00642 COMPLEX1_75K_2, 1 hit PS00643 COMPLEX1_75K_3, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P56914-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MIKVTIDEQS LEVEAGSTVL AAAERLGIEI PTFCYWKRLP PLASCRMCLV 
        60         70         80         90        100
EIEGLRRLQP ACATVAADGM VVRTNTPLIE ETRSSMLDML LANHPLDCPI 
       110        120        130        140        150
CDKGGECELQ DMVMAYGPGE SRFRDPKRVF HSKDIRLSPV IIMNVNRCIQ 
       160        170        180        190        200
CQRCVRMCEE VVGAVALGTV EKGMDTAVTG FEGSLASCDQ CGNCVEVCPV 
       210        220        230        240        250
GALMSFPYRY KARPWDLAET DTICPHCGTG CQLTVGARKG EFMRVRSDWE 
       260        270        280        290        300
HGVNRETLCV RGRFGLDFIE SRDRIKRPMI RRDGTLTPVS WEEAGDFLRQ 
       310        320        330        340        350
RLGVAEGKAA GGLISPRLPN EVLYQFQKLM RTVLRTNNVD CSSRWSAPLD 
       360        370        380        390        400
ILVPIVASFY SRDPLEQVIG KDCVLIIGGN VTEENPVTEY LLRDAARRRH 
       410        420        430        440        450
TRLLMLSARP SRLDADARAV LRAHPGGEGQ SLAAVVAALV AVTDEGLPDD 
       460        470        480        490        500
IFAKTSGTTA SSGANDALDR LVSTLKEGRS VTLLVSVDLL RSPLARKTLE 
       510        520        530        540        550
QLGNLLQLLR LLGKEPSLQF LFDRANQMGA WDMGVLPGVL PGLSPIADEA 
       560        570        580        590        600
TRTRFERSWG AEIPREPGAD VDAMLELCEK GGMGVLYVVG SDPLISYPDR 
       610        620        630        640        650
EFVERALGAA NLLIVQDAFL TDTAGLADVV LPAAGYGEES GTFTNNEGRT 
       660        670        680        690        700
QALRKFREPA FDARSNLAIF GFIAALRERP LQPSTETVIF EEMTRLVPAY 
       710        720        730        740        750
EGLTWEGLGA DGAFTTSAPK PWTSGFFAPL SAPAVTDVLQ LITGNCLFHN 
       760        770        780        790        800
GYVSEHSETL NSVADDPFIE MSAQDAAGLS LSDGDQVLVR SARGELTAKL 
       810        820        830        840        850
KVNRRFPHGL VFVPENYRAL RLNSLMRRGE YPCPVEIREC AKRAASALDE 

ERV

Length:853

Mass (Da):93,346

Last modified:November 2, 2001 - v2

Checksum:ⁱCDF867CE3BD11A0B

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	211	K → R in CAB51635 (Ref. 1) Curated	1
Sequence conflictⁱ	285	T → A in CAB51635 (Ref. 1) Curated	1
Sequence conflictⁱ	452	F → L in CAB51635 (Ref. 1) Curated	1
Sequence conflictⁱ	459	T → I in CAB51635 (Ref. 1) Curated	1
Sequence conflictⁱ	544	S → G in CAB51635 (Ref. 1) Curated	1
Sequence conflictⁱ	554	R → K in CAB51635 (Ref. 1) Curated	1
Sequence conflictⁱ	679	R → Q in CAB51635 (Ref. 1) Curated	1
Sequence conflictⁱ	734	A → G in CAB51635 (Ref. 1) Curated	1
Sequence conflictⁱ	756	H → R in CAB51635 (Ref. 1) Curated	1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AJ245399 Genomic DNA Translation: CAB51635.1 AE006469 Genomic DNA Translation: AAK65486.1
PIRⁱ	D95365
NCBI Reference Sequences More...RefSeqⁱ	NP_436074.1, NC_003037.1 WP_010967796.1, NC_003037.1

Genome annotation databases

EnsemblBacteriaⁱ	AAK65486; AAK65486; SMa1523
GeneIDⁱ	1235864
KEGGⁱ	sme:SMa1523
PATRICⁱ	fig\|266834.11.peg.859

Protein	Similar proteins		Species	Score	Length	Source
P56914	NADH-quinone oxidoreductase		Ensifer sp. MMN_5		853	UniRef90_P56914
NADH-quinone oxidoreductase		SINMM		853
NADH-quinone oxidoreductase		RHIML		853
NADH-quinone oxidoreductase		Sinorhizobium meliloti CCNWSX0020		853
NADH-quinone oxidoreductase		SINMB		853
+25

Protein	Similar proteins		Species	Score	Length	Source
P56914	NADH-quinone oxidoreductase		RHIML		853	UniRef50_P56914
NADH-quinone oxidoreductase		Sinorhizobium sp. M4_45		853
NADH-quinone oxidoreductase		Sinorhizobium meliloti CCNWSX0020		853
NADH-quinone oxidoreductase		Ensifer sp. MMN_5		853
NADH-quinone oxidoreductase		SINMB		853
+93

Entry informationⁱ

Entry nameⁱ	NUOG2_RHIME
Accessionⁱ	P56914Primary (citable) accession number: P56914
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
	Last sequence update:	November 2, 2001
	Last modified:	May 23, 2018
	This is version 125 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Plasmid, Reference proteome

UniProtKB - P56914 (NUOG2_RHIME)

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NADH-quinone oxidoreductase subunit G 2

Select a section on the left to see content.

Functionⁱ

Catalytic activityⁱ

Cofactorⁱ

Sites

GO - Molecular functionⁱ

GO - Biological processⁱ

Keywordsⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

PTM / Processingⁱ

Molecule processing

Keywords - PTMⁱ

Proteomic databases

Structureⁱ

3D structure databases

Family & Domainsⁱ

Domains and Repeats

Sequence similaritiesⁱ

Keywords - Domainⁱ

Phylogenomic databases

Family and domain databases

Sequenceⁱ

Experimental Info

Sequence databases

Genome annotation databases

Similar proteinsⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ

UniProt is an ELIXIR core data resource

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P56914 (NUOG2_RHIME)

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NADH-quinone oxidoreductase subunit G 2

nuoG2

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

GO - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Keywords - PTMi

Proteomic databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ