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Protein

NADH-quinone oxidoreductase subunit G 2

Gene

nuoG2

Organism
Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient (By similarity).By similarity

Catalytic activityi

NADH + a quinone = NAD+ + a quinol.

Cofactori

Protein has several cofactor binding sites:
  • [2Fe-2S] clusterBy similarityNote: Binds 1 [2Fe-2S] cluster per subunit.By similarity
  • [4Fe-4S] clusterBy similarityNote: Binds 3 [4Fe-4S] clusters per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi34Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi45Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi48Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi62Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi94Iron-sulfur 2 (4Fe-4S); via tele nitrogenPROSITE-ProRule annotation1
Metal bindingi98Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi101Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi107Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi148Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi151Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi154Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi198Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi224Iron-sulfur 4 (4Fe-4S)Sequence analysis1
Metal bindingi227Iron-sulfur 4 (4Fe-4S)Sequence analysis1
Metal bindingi231Iron-sulfur 4 (4Fe-4S)Sequence analysis1
Metal bindingi259Iron-sulfur 4 (4Fe-4S)Sequence analysis1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Ligand2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-quinone oxidoreductase subunit G 2 (EC:1.6.5.11)
Alternative name(s):
NADH dehydrogenase I subunit G 2
NDH-1 subunit G 2
Gene namesi
Name:nuoG2
Ordered Locus Names:RA0828
ORF Names:SMa1523
Encoded oniPlasmid pSymA (megaplasmid 1)0 Publication
OrganismiRhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
Taxonomic identifieri266834 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium
Proteomesi
  • UP000001976 Componenti: Plasmid pSymA

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001185601 – 853NADH-quinone oxidoreductase subunit G 2Add BLAST853

Keywords - PTMi

Quinone

Proteomic databases

PRIDEiP56914

Structurei

3D structure databases

ProteinModelPortaliP56914
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 782Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd BLAST78
Domaini78 – 1174Fe-4S His(Cys)3-ligated-typePROSITE-ProRule annotationAdd BLAST40
Domaini139 – 1704Fe-4S ferredoxin-type 1PROSITE-ProRule annotationAdd BLAST32
Domaini179 – 2094Fe-4S ferredoxin-type 2PROSITE-ProRule annotationAdd BLAST31
Domaini217 – 2734Fe-4S Mo/W bis-MGD-typePROSITE-ProRule annotationAdd BLAST57

Sequence similaritiesi

Belongs to the complex I 75 kDa subunit family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

HOGENOMiHOG000031440
KOiK00336
OMAiGYGYVNR

Family and domain databases

CDDicd00207 fer2, 1 hit
InterProiView protein in InterPro
IPR036010 2Fe-2S_ferredoxin-like_sf
IPR001041 2Fe-2S_ferredoxin-type
IPR017896 4Fe4S_Fe-S-bd
IPR017900 4Fe4S_Fe_S_CS
IPR009010 Asp_de-COase-like_dom_sf
IPR006657 MoPterin_dinucl-bd_dom
IPR006656 Mopterin_OxRdtase
IPR006963 Mopterin_OxRdtase_4Fe-4S_dom
IPR027467 MopterinOxRdtase_cofactor_BS
IPR000283 NADH_UbQ_OxRdtase_75kDa_su_CS
IPR010228 NADH_UbQ_OxRdtase_Gsu
IPR019574 NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd
PfamiView protein in Pfam
PF12838 Fer4_7, 1 hit
PF04879 Molybdop_Fe4S4, 1 hit
PF00384 Molybdopterin, 1 hit
PF01568 Molydop_binding, 1 hit
PF10588 NADH-G_4Fe-4S_3, 1 hit
SMARTiView protein in SMART
SM00926 Molybdop_Fe4S4, 1 hit
SM00929 NADH-G_4Fe-4S_3, 1 hit
SUPFAMiSSF50692 SSF50692, 1 hit
SSF54292 SSF54292, 1 hit
TIGRFAMsiTIGR01973 NuoG, 1 hit
PROSITEiView protein in PROSITE
PS51085 2FE2S_FER_2, 1 hit
PS00198 4FE4S_FER_1, 2 hits
PS51379 4FE4S_FER_2, 2 hits
PS51839 4FE4S_HC3, 1 hit
PS51669 4FE4S_MOW_BIS_MGD, 1 hit
PS00641 COMPLEX1_75K_1, 1 hit
PS00642 COMPLEX1_75K_2, 1 hit
PS00643 COMPLEX1_75K_3, 1 hit

Sequencei

Sequence statusi: Complete.

P56914-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MIKVTIDEQS LEVEAGSTVL AAAERLGIEI PTFCYWKRLP PLASCRMCLV
60 70 80 90 100
EIEGLRRLQP ACATVAADGM VVRTNTPLIE ETRSSMLDML LANHPLDCPI
110 120 130 140 150
CDKGGECELQ DMVMAYGPGE SRFRDPKRVF HSKDIRLSPV IIMNVNRCIQ
160 170 180 190 200
CQRCVRMCEE VVGAVALGTV EKGMDTAVTG FEGSLASCDQ CGNCVEVCPV
210 220 230 240 250
GALMSFPYRY KARPWDLAET DTICPHCGTG CQLTVGARKG EFMRVRSDWE
260 270 280 290 300
HGVNRETLCV RGRFGLDFIE SRDRIKRPMI RRDGTLTPVS WEEAGDFLRQ
310 320 330 340 350
RLGVAEGKAA GGLISPRLPN EVLYQFQKLM RTVLRTNNVD CSSRWSAPLD
360 370 380 390 400
ILVPIVASFY SRDPLEQVIG KDCVLIIGGN VTEENPVTEY LLRDAARRRH
410 420 430 440 450
TRLLMLSARP SRLDADARAV LRAHPGGEGQ SLAAVVAALV AVTDEGLPDD
460 470 480 490 500
IFAKTSGTTA SSGANDALDR LVSTLKEGRS VTLLVSVDLL RSPLARKTLE
510 520 530 540 550
QLGNLLQLLR LLGKEPSLQF LFDRANQMGA WDMGVLPGVL PGLSPIADEA
560 570 580 590 600
TRTRFERSWG AEIPREPGAD VDAMLELCEK GGMGVLYVVG SDPLISYPDR
610 620 630 640 650
EFVERALGAA NLLIVQDAFL TDTAGLADVV LPAAGYGEES GTFTNNEGRT
660 670 680 690 700
QALRKFREPA FDARSNLAIF GFIAALRERP LQPSTETVIF EEMTRLVPAY
710 720 730 740 750
EGLTWEGLGA DGAFTTSAPK PWTSGFFAPL SAPAVTDVLQ LITGNCLFHN
760 770 780 790 800
GYVSEHSETL NSVADDPFIE MSAQDAAGLS LSDGDQVLVR SARGELTAKL
810 820 830 840 850
KVNRRFPHGL VFVPENYRAL RLNSLMRRGE YPCPVEIREC AKRAASALDE

ERV
Length:853
Mass (Da):93,346
Last modified:November 2, 2001 - v2
Checksum:iCDF867CE3BD11A0B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti211K → R in CAB51635 (Ref. 1) Curated1
Sequence conflicti285T → A in CAB51635 (Ref. 1) Curated1
Sequence conflicti452F → L in CAB51635 (Ref. 1) Curated1
Sequence conflicti459T → I in CAB51635 (Ref. 1) Curated1
Sequence conflicti544S → G in CAB51635 (Ref. 1) Curated1
Sequence conflicti554R → K in CAB51635 (Ref. 1) Curated1
Sequence conflicti679R → Q in CAB51635 (Ref. 1) Curated1
Sequence conflicti734A → G in CAB51635 (Ref. 1) Curated1
Sequence conflicti756H → R in CAB51635 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ245399 Genomic DNA Translation: CAB51635.1
AE006469 Genomic DNA Translation: AAK65486.1
PIRiD95365
RefSeqiNP_436074.1, NC_003037.1
WP_010967796.1, NC_003037.1

Genome annotation databases

EnsemblBacteriaiAAK65486; AAK65486; SMa1523
GeneIDi1235864
KEGGisme:SMa1523
PATRICifig|266834.11.peg.859

Similar proteinsi

Entry informationi

Entry nameiNUOG2_RHIME
AccessioniPrimary (citable) accession number: P56914
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 2, 2001
Last modified: May 23, 2018
This is version 125 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Plasmid, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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