UniProtKB - P56868 (MURI_AQUPY)
Protein
Glutamate racemase
Gene
murI
Organism
Aquifex pyrophilus
Status
Functioni
Provides the (R)-glutamate required for cell wall biosynthesis. Converts L- or D-glutamate to D- or L-glutamate, respectively, but not other amino acids such as alanine, aspartate, and glutamine.
Catalytic activityi
- EC:5.1.1.3UniRule annotation2 Publications
Temperature dependencei
Thermostable.
: peptidoglycan biosynthesis Pathwayi
This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotationView all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 70 | Proton donor/acceptorUniRule annotationBy similarity | 1 | |
Binding sitei | 147 | SubstrateCombined sources1 Publication | 1 | |
Active sitei | 178 | Proton donor/acceptorUniRule annotationBy similarity | 1 |
GO - Molecular functioni
- glutamate racemase activity Source: UniProtKB-UniRule
GO - Biological processi
- cell wall organization Source: UniProtKB-KW
- peptidoglycan biosynthetic process Source: UniProtKB-UniRule
- regulation of cell shape Source: UniProtKB-KW
Keywordsi
Molecular function | Isomerase |
Biological process | Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis |
Enzyme and pathway databases
BRENDAi | 5.1.1.3, 397 |
SABIO-RKi | P56868 |
UniPathwayi | UPA00219 |
Names & Taxonomyi
Protein namesi | Recommended name: Glutamate racemase1 PublicationUniRule annotation (EC:5.1.1.3UniRule annotation2 Publications) |
Gene namesi | Name:murIUniRule annotation |
Organismi | Aquifex pyrophilus |
Taxonomic identifieri | 2714 [NCBI] |
Taxonomic lineagei | Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex |
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 7 | D → S: Strongly reduced catalytic activity. 1 Publication | 1 | |
Mutagenesisi | 147 | E → N: Strongly reduced catalytic activity. 1 Publication | 1 |
Chemistry databases
DrugBanki | DB02174, D-Glutamine |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000095449 | 1 – 254 | Glutamate racemaseAdd BLAST | 254 |
Interactioni
Subunit structurei
Homodimer.
2 PublicationsStructurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P56868 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P56868 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 7 – 8 | Substrate bindingUniRule annotationBy similarity | 2 | |
Regioni | 39 – 40 | Substrate bindingUniRule annotationBy similarity | 2 | |
Regioni | 71 – 72 | Substrate bindingUniRule annotationCombined sources1 Publication | 2 | |
Regioni | 179 – 180 | Substrate bindingUniRule annotationBy similarity | 2 |
Sequence similaritiesi
Belongs to the aspartate/glutamate racemases family.UniRule annotation
Family and domain databases
HAMAPi | MF_00258, Glu_racemase, 1 hit |
InterProi | View protein in InterPro IPR015942, Asp/Glu/hydantoin_racemase IPR001920, Asp/Glu_race IPR018187, Asp/Glu_racemase_AS_1 IPR033134, Asp/Glu_racemase_AS_2 IPR004391, Glu_race |
PANTHERi | PTHR21198:SF2, PTHR21198:SF2, 1 hit |
Pfami | View protein in Pfam PF01177, Asp_Glu_race, 1 hit |
SUPFAMi | SSF53681, SSF53681, 2 hits |
TIGRFAMsi | TIGR00067, glut_race, 1 hit |
PROSITEi | View protein in PROSITE PS00923, ASP_GLU_RACEMASE_1, 1 hit PS00924, ASP_GLU_RACEMASE_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P56868-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKIGIFDSGV GGLTVLKAIR NRYRKVDIVY LGDTARVPYG IRSKDTIIRY
60 70 80 90 100
SLECAGFLKD KGVDIIVVAC NTASAYALER LKKEINVPVF GVIEPGVKEA
110 120 130 140 150
LKKSRNKKIG VIGTPATVKS GAYQRKLEEG GADVFAKACP LFVPLAEEGL
160 170 180 190 200
LEGEITRKVV EHYLKEFKGK IDTLILGCTH YPLLKKEIKK FLGDVEVVDS
210 220 230 240 250
SEALSLSLHN FIKDDGSSSL ELFFTDLSPN LQFLIKLILG RDYPVKLAEG
VFTH
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF212972 Genomic DNA Translation: AAF25672.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF212972 Genomic DNA Translation: AAF25672.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1B73 | X-ray | 2.30 | A | 1-254 | [»] | |
1B74 | X-ray | 2.30 | A | 1-254 | [»] | |
SMRi | P56868 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Chemistry databases
DrugBanki | DB02174, D-Glutamine |
Enzyme and pathway databases
UniPathwayi | UPA00219 |
BRENDAi | 5.1.1.3, 397 |
SABIO-RKi | P56868 |
Miscellaneous databases
EvolutionaryTracei | P56868 |
Family and domain databases
HAMAPi | MF_00258, Glu_racemase, 1 hit |
InterProi | View protein in InterPro IPR015942, Asp/Glu/hydantoin_racemase IPR001920, Asp/Glu_race IPR018187, Asp/Glu_racemase_AS_1 IPR033134, Asp/Glu_racemase_AS_2 IPR004391, Glu_race |
PANTHERi | PTHR21198:SF2, PTHR21198:SF2, 1 hit |
Pfami | View protein in Pfam PF01177, Asp_Glu_race, 1 hit |
SUPFAMi | SSF53681, SSF53681, 2 hits |
TIGRFAMsi | TIGR00067, glut_race, 1 hit |
PROSITEi | View protein in PROSITE PS00923, ASP_GLU_RACEMASE_1, 1 hit PS00924, ASP_GLU_RACEMASE_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MURI_AQUPY | |
Accessioni | P56868Primary (citable) accession number: P56868 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | May 30, 2000 | |
Last modified: | December 11, 2019 | |
This is version 92 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structureDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families