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Protein

Proto-oncogene Wnt-3

Gene

WNT3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family (PubMed:26902720). Required for normal gastrulation, formation of the primitive streak, and for the formation of the mesoderm during early embryogenesis. Required for normal formation of the apical ectodermal ridge (By similarity). Required for normal embryonic development, and especially for limb development (PubMed:14872406).By similarityCurated2 Publications

GO - Molecular functioni

  • frizzled binding Source: UniProtKB
  • protein domain specific binding Source: Ensembl
  • receptor ligand activity Source: ParkinsonsUK-UCL

GO - Biological processi

  • anterior/posterior axis specification Source: Ensembl
  • axon guidance Source: Ensembl
  • canonical Wnt signaling pathway Source: UniProtKB
  • canonical Wnt signaling pathway involved in mesenchymal stem cell differentiation Source: BHF-UCL
  • canonical Wnt signaling pathway involved in midbrain dopaminergic neuron differentiation Source: ParkinsonsUK-UCL
  • canonical Wnt signaling pathway involved in osteoblast differentiation Source: BHF-UCL
  • canonical Wnt signaling pathway involved in stem cell proliferation Source: ParkinsonsUK-UCL
  • cell fate commitment Source: GO_Central
  • cell morphogenesis Source: BHF-UCL
  • cellular response to retinoic acid Source: UniProtKB
  • dorsal/ventral axis specification Source: Ensembl
  • embryonic forelimb morphogenesis Source: Ensembl
  • embryonic hindlimb morphogenesis Source: Ensembl
  • gamete generation Source: Ensembl
  • head morphogenesis Source: Ensembl
  • limb bud formation Source: BHF-UCL
  • mammary gland epithelium development Source: UniProtKB
  • mesoderm formation Source: Ensembl
  • negative regulation of axon extension involved in axon guidance Source: Ensembl
  • neuron differentiation Source: UniProtKB
  • positive regulation of collateral sprouting in absence of injury Source: Ensembl
  • positive regulation of gene expression Source: ParkinsonsUK-UCL
  • positive regulation of Wnt signaling pathway Source: Ensembl
  • regulation of neurogenesis Source: ParkinsonsUK-UCL
  • Spemann organizer formation at the anterior end of the primitive streak Source: Ensembl
  • stem cell proliferation Source: ParkinsonsUK-UCL
  • Wnt signaling pathway Source: Reactome

Keywordsi

Molecular functionDevelopmental protein
Biological processWnt signaling pathway

Enzyme and pathway databases

ReactomeiR-HSA-201681 TCF dependent signaling in response to WNT
R-HSA-3238698 WNT ligand biogenesis and trafficking
R-HSA-373080 Class B/2 (Secretin family receptors)
SIGNORiP56703

Names & Taxonomyi

Protein namesi
Recommended name:
Proto-oncogene Wnt-3
Alternative name(s):
Proto-oncogene Int-4 homolog
Gene namesi
Name:WNT3
Synonyms:INT4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

EuPathDBiHostDB:ENSG00000108379.9
HGNCiHGNC:12782 WNT3
MIMi165330 gene
neXtProtiNX_P56703

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Extracellular matrix, Secreted

Pathology & Biotechi

Involvement in diseasei

Tetraamelia syndrome, autosomal recessive (TETAMS)1 Publication
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionA rare human genetic disorder characterized by complete absence of all four limbs and other anomalies such as craniofacial, nervous system, pulmonary, skeletal and urogenital defects.
See also OMIM:273395

Keywords - Diseasei

Proto-oncogene

Organism-specific databases

DisGeNETi7473
GeneReviewsiWNT3
MalaCardsiWNT3
MIMi273395 phenotype
OpenTargetsiENSG00000108379
Orphaneti3301 Tetraamelia - multiple malformations
PharmGKBiPA37383

Chemistry databases

ChEMBLiCHEMBL6079

Polymorphism and mutation databases

DMDMi14424477

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000004141622 – 355Proto-oncogene Wnt-3Add BLAST334

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi80 ↔ 91By similarity
Glycosylationi90N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi131 ↔ 139By similarity
Disulfide bondi141 ↔ 158By similarity
Disulfide bondi206 ↔ 220By similarity
Disulfide bondi208 ↔ 215By similarity
Lipidationi212O-palmitoleoyl serine; by PORCNBy similarity1
Disulfide bondi300 ↔ 315By similarity
Glycosylationi301N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi330 ↔ 345By similarity
Disulfide bondi332 ↔ 342By similarity
Disulfide bondi337 ↔ 338By similarity

Post-translational modificationi

Palmitoleoylation is required for efficient binding to frizzled receptors. Depalmitoleoylation leads to Wnt signaling pathway inhibition.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein

Proteomic databases

EPDiP56703
PaxDbiP56703
PeptideAtlasiP56703
PRIDEiP56703
ProteomicsDBi56936

PTM databases

iPTMnetiP56703
PhosphoSitePlusiP56703

Expressioni

Gene expression databases

BgeeiENSG00000108379
CleanExiHS_WNT3
GenevisibleiP56703 HS

Interactioni

Subunit structurei

Forms a soluble 1:1 complex with AFM; this prevents oligomerization and is required for prolonged biological activity (PubMed:26902720). The complex with AFM may represent the physiological form in body fluids (PubMed:26902720).Interacts with PORCN. Interacts with WLS (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
FZD7O750843EBI-3644922,EBI-746917

GO - Molecular functioni

  • frizzled binding Source: UniProtKB
  • protein domain specific binding Source: Ensembl
  • receptor ligand activity Source: ParkinsonsUK-UCL

Protein-protein interaction databases

BioGridi113310, 1 interactor
IntActiP56703, 1 interactor
STRINGi9606.ENSP00000225512

Chemistry databases

BindingDBiP56703

Structurei

3D structure databases

ProteinModelPortaliP56703
SMRiP56703
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Wnt family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3913 Eukaryota
ENOG410XQZ1 LUCA
GeneTreeiENSGT00760000118943
HOGENOMiHOG000039529
HOVERGENiHBG001595
InParanoidiP56703
KOiK00312
OMAiMCGCDSH
OrthoDBiEOG091G0OFF
PhylomeDBiP56703
TreeFamiTF105310

Family and domain databases

InterProiView protein in InterPro
IPR005817 Wnt
IPR009141 Wnt3
IPR018161 Wnt_CS
PANTHERiPTHR12027 PTHR12027, 1 hit
PfamiView protein in Pfam
PF00110 wnt, 1 hit
PRINTSiPR01843 WNT3PROTEIN
PR01349 WNTPROTEIN
SMARTiView protein in SMART
SM00097 WNT1, 1 hit
PROSITEiView protein in PROSITE
PS00246 WNT1, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P56703-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPHLLGLLL GLLLGGTRVL AGYPIWWSLA LGQQYTSLGS QPLLCGSIPG
60 70 80 90 100
LVPKQLRFCR NYIEIMPSVA EGVKLGIQEC QHQFRGRRWN CTTIDDSLAI
110 120 130 140 150
FGPVLDKATR ESAFVHAIAS AGVAFAVTRS CAEGTSTICG CDSHHKGPPG
160 170 180 190 200
EGWKWGGCSE DADFGVLVSR EFADARENRP DARSAMNKHN NEAGRTTILD
210 220 230 240 250
HMHLKCKCHG LSGSCEVKTC WWAQPDFRAI GDFLKDKYDS ASEMVVEKHR
260 270 280 290 300
ESRGWVETLR AKYSLFKPPT ERDLVYYENS PNFCEPNPET GSFGTRDRTC
310 320 330 340 350
NVTSHGIDGC DLLCCGRGHN TRTEKRKEKC HCIFHWCCYV SCQECIRIYD

VHTCK
Length:355
Mass (Da):39,645
Last modified:June 20, 2001 - v2
Checksum:i85D15F2C7884A64F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY009397 mRNA Translation: AAG38657.1
AB067628 mRNA Translation: BAB70502.1
BC112116 mRNA Translation: AAI12117.1
BC112118 mRNA Translation: AAI12119.1
BC114219 mRNA Translation: AAI14220.1
CCDSiCCDS11505.1
PIRiA47536
RefSeqiNP_110380.1, NM_030753.4
UniGeneiHs.445884
Hs.745220

Genome annotation databases

EnsembliENST00000225512; ENSP00000225512; ENSG00000108379
ENST00000611547; ENSP00000478327; ENSG00000277626
ENST00000616347; ENSP00000480990; ENSG00000277641
GeneIDi7473
KEGGihsa:7473
UCSCiuc002ikv.3 human

Similar proteinsi

Entry informationi

Entry nameiWNT3_HUMAN
AccessioniPrimary (citable) accession number: P56703
Secondary accession number(s): Q2M237, Q9H1J9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: June 20, 2001
Last modified: July 18, 2018
This is version 155 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

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