WNT3

Functionⁱ

Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family (PubMed:26902720). Required for normal gastrulation, formation of the primitive streak, and for the formation of the mesoderm during early embryogenesis. Required for normal formation of the apical ectodermal ridge (By similarity). Required for normal embryonic development, and especially for limb development (PubMed:14872406).By similarityCurated2 Publications

GO - Molecular functionⁱ

frizzled binding
Source: UniProtKB
Inferred from physical interactionⁱ
- 10557084
protein domain specific binding Source: Ensembl
receptor ligand activity
Source: ParkinsonsUK-UCL
Inferred from direct assayⁱ
- 10557084

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

anterior/posterior axis specification Source: Ensembl
axon guidance Source: Ensembl
canonical Wnt signaling pathway
Source: UniProtKB
Inferred from direct assayⁱ
- 10557084
canonical Wnt signaling pathway involved in mesenchymal stem cell differentiation
Source: BHF-UCL
Inferred from mutant phenotypeⁱ
- 20039315
canonical Wnt signaling pathway involved in midbrain dopaminergic neuron differentiation
Source: ParkinsonsUK-UCL
Inferred from mutant phenotypeⁱ
- 25640183
canonical Wnt signaling pathway involved in osteoblast differentiation
Source: BHF-UCL
Inferred from mutant phenotypeⁱ
- 20039315
canonical Wnt signaling pathway involved in stem cell proliferation
Source: ParkinsonsUK-UCL
Inferred from direct assayⁱ
- 25640183
cell fate commitment
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
cell morphogenesis
Source: BHF-UCL
Inferred from mutant phenotypeⁱ
- 15588944
cellular response to retinoic acid Source: UniProtKB
dorsal/ventral axis specification Source: Ensembl
embryonic forelimb morphogenesis Source: Ensembl
embryonic hindlimb morphogenesis Source: Ensembl
gamete generation Source: Ensembl
head morphogenesis Source: Ensembl
limb bud formation
Source: BHF-UCL
Inferred from mutant phenotypeⁱ
- 14872406
mammary gland epithelium development
Source: UniProtKB
Inferred from expression patternⁱ
- 8168088
mesoderm formation Source: Ensembl
negative regulation of axon extension involved in axon guidance Source: Ensembl
neuron differentiation Source: UniProtKB
positive regulation of collateral sprouting in absence of injury Source: Ensembl
positive regulation of gene expression
Source: ParkinsonsUK-UCL
Inferred from direct assayⁱ
- 25640183
positive regulation of Wnt signaling pathway Source: Ensembl
regulation of neurogenesis
Source: ParkinsonsUK-UCL
Inferred from mutant phenotypeⁱ
- 25640183
Spemann organizer formation at the anterior end of the primitive streak Source: Ensembl
stem cell proliferation
Source: ParkinsonsUK-UCL
Inferred from mutant phenotypeⁱ
- 25640183
Wnt signaling pathway
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Developmental protein
Biological process	Wnt signaling pathway

Enzyme and pathway databases

Reactomeⁱ	R-HSA-201681 TCF dependent signaling in response to WNT R-HSA-3238698 WNT ligand biogenesis and trafficking R-HSA-373080 Class B/2 (Secretin family receptors)
SIGNORⁱ	P56703

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Proto-oncogene Wnt-3 Alternative name(s): Proto-oncogene Int-4 homolog
Gene namesⁱ	Name:WNT3 Synonyms:INT4
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 17

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000108379.9
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:12782 WNT3
MIMⁱ	165330 gene
neXtProtⁱ	NX_P56703

Keywords - Cellular componentⁱ

Extracellular matrix, Secreted

Pathology & Biotechⁱ

Involvement in diseaseⁱ

Tetraamelia syndrome 1 (TETAMS1)1 Publication

The disease is caused by mutations affecting the gene represented in this entry.

Disease descriptionA form of tetraamelia, a rare disease characterized by rudimentary appendages or complete absence of all four limbs, and other anomalies such as craniofacial, nervous system, pulmonary, skeletal and urogenital defects. TETAMS1 patients manifest complete limb agenesis without defects of scapulae or clavicles. Other features include bilateral cleft lip/palate, diaphragmatic defect with bilobar right lung, renal and adrenal agenesis, pelvic hypoplasia, and urogenital defects. TETAMS1 transmission pattern is consistent with autosomal recessive inheritance.

Keywords - Diseaseⁱ

Proto-oncogene

Organism-specific databases

DisGeNET More...DisGeNETⁱ	7473
GeneReviewsⁱ	WNT3
MalaCards human disease database More...MalaCardsⁱ	WNT3
MIMⁱ	273395 phenotype
Open Targets More...OpenTargetsⁱ	ENSG00000108379
Orphanetⁱ	3301 Tetraamelia-multiple malformations syndrome
PharmGKBⁱ	PA37383

Chemistry databases

ChEMBLⁱ	CHEMBL6079

ChEMBLⁱ

CHEMBL6079

Polymorphism and mutation databases

DMDMⁱ	14424477

DMDMⁱ

14424477

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Signal peptideⁱ	1 – 21	Sequence analysisAdd BLAST		21
ChainⁱPRO_0000041416	22 – 355	Proto-oncogene Wnt-3Add BLAST		334

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Disulfide bondⁱ	80 ↔ 91	By similarity
Glycosylationⁱ	90	N-linked (GlcNAc...) asparagineSequence analysis	1
Disulfide bondⁱ	131 ↔ 139	By similarity
Disulfide bondⁱ	141 ↔ 158	By similarity
Disulfide bondⁱ	206 ↔ 220	By similarity
Disulfide bondⁱ	208 ↔ 215	By similarity
Lipidationⁱ	212	O-palmitoleoyl serine; by PORCNBy similarity	1
Disulfide bondⁱ	300 ↔ 315	By similarity
Glycosylationⁱ	301	N-linked (GlcNAc...) asparagineSequence analysis	1
Disulfide bondⁱ	330 ↔ 345	By similarity
Disulfide bondⁱ	332 ↔ 342	By similarity
Disulfide bondⁱ	337 ↔ 338	By similarity

Post-translational modificationⁱ

Palmitoleoylation is required for efficient binding to frizzled receptors. Depalmitoleoylation leads to Wnt signaling pathway inhibition.By similarity

Keywords - PTMⁱ

Disulfide bond, Glycoprotein, Lipoprotein

Proteomic databases

EPDⁱ	P56703
PaxDbⁱ	P56703
PeptideAtlas More...PeptideAtlasⁱ	P56703
PRIDEⁱ	P56703
ProteomicsDBⁱ	56936

PTM databases

iPTMnetⁱ	P56703
PhosphoSitePlusⁱ	P56703

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSG00000108379 Expressed in 131 organ(s), highest expression level in skin of abdomen
CleanExⁱ	HS_WNT3
Genevisibleⁱ	P56703 HS

Interactionⁱ

Subunit structureⁱ

Forms a soluble 1:1 complex with AFM; this prevents oligomerization and is required for prolonged biological activity (PubMed:26902720). The complex with AFM may represent the physiological form in body fluids (PubMed:26902720).Interacts with PORCN. Interacts with WLS (By similarity).By similarity

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
FZD7	O75084	3	EBI-3644922,EBI-746917

With

Entry

#Exp.

IntAct

Notes

FZD7

O75084

3

EBI-3644922,EBI-746917

GO - Molecular functionⁱ

frizzled binding
Source: UniProtKB
Inferred from physical interactionⁱ
- 10557084
protein domain specific binding Source: Ensembl
receptor ligand activity
Source: ParkinsonsUK-UCL
Inferred from direct assayⁱ
- 10557084

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	113310, 1 interactor
IntActⁱ	P56703, 1 interactor
STRINGⁱ	9606.ENSP00000225512

Chemistry databases

BindingDBⁱ

P56703

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P56703
SMRⁱ	P56703
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the Wnt family.Curated

Keywords - Domainⁱ

Signal

Phylogenomic databases

eggNOGⁱ	KOG3913 Eukaryota ENOG410XQZ1 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00760000118943
HOGENOMⁱ	HOG000039529
HOVERGENⁱ	HBG001595
InParanoidⁱ	P56703
KEGG Orthology (KO) More...KOⁱ	K00312
OMAⁱ	MCGCDSH
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0OFF
PhylomeDBⁱ	P56703
TreeFamⁱ	TF105310

Family and domain databases

InterProⁱ	View protein in InterPro IPR005817 Wnt IPR009141 Wnt3 IPR018161 Wnt_CS
PANTHERⁱ	PTHR12027 PTHR12027, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00110 wnt, 1 hit
PRINTSⁱ	PR01843 WNT3PROTEIN PR01349 WNTPROTEIN
SMARTⁱ	View protein in SMART SM00097 WNT1, 1 hit
PROSITEⁱ	View protein in PROSITE PS00246 WNT1, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

P56703-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MEPHLLGLLL GLLLGGTRVL AGYPIWWSLA LGQQYTSLGS QPLLCGSIPG 
        60         70         80         90        100
LVPKQLRFCR NYIEIMPSVA EGVKLGIQEC QHQFRGRRWN CTTIDDSLAI 
       110        120        130        140        150
FGPVLDKATR ESAFVHAIAS AGVAFAVTRS CAEGTSTICG CDSHHKGPPG 
       160        170        180        190        200
EGWKWGGCSE DADFGVLVSR EFADARENRP DARSAMNKHN NEAGRTTILD 
       210        220        230        240        250
HMHLKCKCHG LSGSCEVKTC WWAQPDFRAI GDFLKDKYDS ASEMVVEKHR 
       260        270        280        290        300
ESRGWVETLR AKYSLFKPPT ERDLVYYENS PNFCEPNPET GSFGTRDRTC 
       310        320        330        340        350
NVTSHGIDGC DLLCCGRGHN TRTEKRKEKC HCIFHWCCYV SCQECIRIYD 

VHTCK

Length:355

Mass (Da):39,645

Last modified:June 20, 2001 - v2

Checksum:ⁱ85D15F2C7884A64F

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AY009397 mRNA Translation: AAG38657.1 AB067628 mRNA Translation: BAB70502.1 BC112116 mRNA Translation: AAI12117.1 BC112118 mRNA Translation: AAI12119.1 BC114219 mRNA Translation: AAI14220.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS11505.1
PIRⁱ	A47536
NCBI Reference Sequences More...RefSeqⁱ	NP_110380.1, NM_030753.4
UniGeneⁱ	Hs.445884 Hs.745220

Genome annotation databases

Ensemblⁱ	ENST00000225512; ENSP00000225512; ENSG00000108379 ENST00000611547; ENSP00000478327; ENSG00000277626 ENST00000616347; ENSP00000480990; ENSG00000277641
GeneIDⁱ	7473
KEGGⁱ	hsa:7473
UCSC genome browser More...UCSCⁱ	uc002ikv.3 human

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
P56703	Protein Wnt		PANTR		290	UniRef100_P56703
Protein Wnt		MACMU		170
Protein Wnt		PONAB		355
Protein Wnt		COLAP		355
Protein Wnt		CERAT		355
+12

Protein	Similar proteins		Species	Score	Length	Source
P56703	Protein Wnt		PANTR		290	UniRef90_P56703
Protein Wnt		MACMU		170
Protein Wnt		PONAB		355
Protein Wnt		COLAP		355
Protein Wnt		CERAT		355
+35

Protein	Similar proteins		Species	Score	Length	Source
P56703	Protein Wnt		PANTR		290	UniRef50_P56703
Protein Wnt		MACMU		170
Protein Wnt		PONAB		355
Protein Wnt		COLAP		355
Protein Wnt		CERAT		355
+61

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AY009397 mRNA Translation: AAG38657.1 AB067628 mRNA Translation: BAB70502.1 BC112116 mRNA Translation: AAI12117.1 BC112118 mRNA Translation: AAI12119.1 BC114219 mRNA Translation: AAI14220.1
CCDSⁱ	CCDS11505.1
PIRⁱ	A47536
RefSeqⁱ	NP_110380.1, NM_030753.4
UniGeneⁱ	Hs.445884 Hs.745220

3D structure databases

ProteinModelPortalⁱ	P56703
SMRⁱ	P56703
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	113310, 1 interactor
IntActⁱ	P56703, 1 interactor
STRINGⁱ	9606.ENSP00000225512

Chemistry databases

BindingDBⁱ	P56703
ChEMBLⁱ	CHEMBL6079

PTM databases

iPTMnetⁱ	P56703
PhosphoSitePlusⁱ	P56703

Polymorphism and mutation databases

DMDMⁱ	14424477

DMDMⁱ

14424477

Proteomic databases

EPDⁱ	P56703
PaxDbⁱ	P56703
PeptideAtlasⁱ	P56703
PRIDEⁱ	P56703
ProteomicsDBⁱ	56936

Protocols and materials databases

The DNASU plasmid repository More...DNASUⁱ	7473
Structural Biology Knowledgebase	Search...

Genome annotation databases

Ensemblⁱ	ENST00000225512; ENSP00000225512; ENSG00000108379 ENST00000611547; ENSP00000478327; ENSG00000277626 ENST00000616347; ENSP00000480990; ENSG00000277641
GeneIDⁱ	7473
KEGGⁱ	hsa:7473
UCSCⁱ	uc002ikv.3 human

Organism-specific databases

CTDⁱ	7473
DisGeNETⁱ	7473
EuPathDBⁱ	HostDB:ENSG00000108379.9
GeneCardsⁱ	WNT3
GeneReviewsⁱ	WNT3
HGNCⁱ	HGNC:12782 WNT3
MalaCardsⁱ	WNT3
MIMⁱ	165330 gene 273395 phenotype
neXtProtⁱ	NX_P56703
OpenTargetsⁱ	ENSG00000108379
Orphanetⁱ	3301 Tetraamelia-multiple malformations syndrome
PharmGKBⁱ	PA37383
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	KOG3913 Eukaryota ENOG410XQZ1 LUCA
GeneTreeⁱ	ENSGT00760000118943
HOGENOMⁱ	HOG000039529
HOVERGENⁱ	HBG001595
InParanoidⁱ	P56703
KOⁱ	K00312
OMAⁱ	MCGCDSH
OrthoDBⁱ	EOG091G0OFF
PhylomeDBⁱ	P56703
TreeFamⁱ	TF105310

Enzyme and pathway databases

Reactomeⁱ	R-HSA-201681 TCF dependent signaling in response to WNT R-HSA-3238698 WNT ligand biogenesis and trafficking R-HSA-373080 Class B/2 (Secretin family receptors)
SIGNORⁱ	P56703

Miscellaneous databases

GeneWikiⁱ	WNT3
GenomeRNAiⁱ	7473
Protein Ontology More...PROⁱ	PR:P56703
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSG00000108379 Expressed in 131 organ(s), highest expression level in skin of abdomen
CleanExⁱ	HS_WNT3
Genevisibleⁱ	P56703 HS

Family and domain databases

InterProⁱ	View protein in InterPro IPR005817 Wnt IPR009141 Wnt3 IPR018161 Wnt_CS
PANTHERⁱ	PTHR12027 PTHR12027, 1 hit
Pfamⁱ	View protein in Pfam PF00110 wnt, 1 hit
PRINTSⁱ	PR01843 WNT3PROTEIN PR01349 WNTPROTEIN
SMARTⁱ	View protein in SMART SM00097 WNT1, 1 hit
PROSITEⁱ	View protein in PROSITE PS00246 WNT1, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	WNT3_HUMAN
Accessionⁱ	P56703Primary (citable) accession number: P56703 Secondary accession number(s): Q2M237, Q9H1J9
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
	Last sequence update:	June 20, 2001
	Last modified:	November 7, 2018
	This is version 158 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
Human chromosome 17
Human chromosome 17: entries, gene names and cross-references to MIM

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P56703 (WNT3_HUMAN)

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Proto-oncogene Wnt-3

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted

Endoplasmic reticulum

Extracellular region or secreted

Golgi apparatus

Plasma Membrane

Other locations

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Keywords - Diseasei

Organism-specific databases

Chemistry databases

Polymorphism and mutation databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

Chemistry databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Chemistry databases

PTM databases

Polymorphism and mutation databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

UniProt is an ELIXIR core data resource

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

Keywords - Diseaseⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ