UniProtKB - P56696 (KCNQ4_HUMAN)
Protein
Potassium voltage-gated channel subfamily KQT member 4
Gene
KCNQ4
Organism
Homo sapiens (Human)
Status
Functioni
Probably important in the regulation of neuronal excitability. May underlie a potassium current involved in regulating the excitability of sensory cells of the cochlea. KCNQ4 channels are blocked by linopirdin, XE991 and bepridil, whereas clofilium is without significant effect. Muscarinic agonist oxotremorine-M strongly suppress KCNQ4 current in CHO cells in which cloned KCNQ4 channels were coexpressed with M1 muscarinic receptors.
Miscellaneous
Mutagenesis experiments were carried out by expressing in Xenopus oocytes KCNQ4 mutants either individually (homomultimers) or in combination with wild-type KCNQ4 (mut/wt homomultimers) in a ratio of 1:1, to mimic the situation in a heterozygous DFNA2 patient.
GO - Molecular functioni
- potassium channel activity Source: ProtInc
- voltage-gated potassium channel activity Source: GO_Central
GO - Biological processi
- potassium ion transport Source: ProtInc
- regulation of ion transmembrane transport Source: UniProtKB-KW
- sensory perception of sound Source: ProtInc
Keywordsi
| Molecular function | Ion channel, Potassium channel, Voltage-gated channel |
| Biological process | Hearing, Ion transport, Potassium transport, Transport |
| Ligand | Potassium |
Enzyme and pathway databases
| Reactomei | R-HSA-1296072 Voltage gated Potassium channels |
Protein family/group databases
| TCDBi | 1.A.1.15.4 the voltage-gated ion channel (vic) superfamily |
Names & Taxonomyi
| Protein namesi | Recommended name: Potassium voltage-gated channel subfamily KQT member 4Alternative name(s): KQT-like 4 Potassium channel subunit alpha KvLQT4 Voltage-gated potassium channel subunit Kv7.4 |
| Gene namesi | Name:KCNQ4 |
| Organismi | Homo sapiens (Human) |
| Taxonomic identifieri | 9606 [NCBI] |
| Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
| Proteomesi |
|
Organism-specific databases
| EuPathDBi | HostDB:ENSG00000117013.14 |
| HGNCi | HGNC:6298 KCNQ4 |
| MIMi | 603537 gene |
| neXtProti | NX_P56696 |
Subcellular locationi
Topology
| Feature key | Position(s) | DescriptionActions | Graphical view | Length |
|---|---|---|---|---|
| Topological domaini | 1 – 97 | CytoplasmicSequence analysisAdd BLAST | 97 | |
| Transmembranei | 98 – 118 | Helical; Name=Segment S1Sequence analysisAdd BLAST | 21 | |
| Topological domaini | 119 – 131 | ExtracellularSequence analysisAdd BLAST | 13 | |
| Transmembranei | 132 – 152 | Helical; Name=Segment S2Sequence analysisAdd BLAST | 21 | |
| Topological domaini | 153 – 172 | CytoplasmicSequence analysisAdd BLAST | 20 | |
| Transmembranei | 173 – 193 | Helical; Name=Segment S3Sequence analysisAdd BLAST | 21 | |
| Topological domaini | 194 – 201 | ExtracellularSequence analysis | 8 | |
| Transmembranei | 202 – 224 | Helical; Voltage-sensor; Name=Segment S4Sequence analysisAdd BLAST | 23 | |
| Topological domaini | 225 – 237 | CytoplasmicSequence analysisAdd BLAST | 13 | |
| Transmembranei | 238 – 258 | Helical; Name=Segment S5Sequence analysisAdd BLAST | 21 | |
| Topological domaini | 259 – 270 | ExtracellularSequence analysisAdd BLAST | 12 | |
| Intramembranei | 271 – 292 | Pore-forming; Name=Segment H5Sequence analysisAdd BLAST | 22 | |
| Topological domaini | 293 – 296 | ExtracellularSequence analysis | 4 | |
| Transmembranei | 297 – 317 | Helical; Name=Segment S6Sequence analysisAdd BLAST | 21 | |
| Topological domaini | 318 – 695 | CytoplasmicSequence analysisAdd BLAST | 378 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Involvement in diseasei
Deafness, autosomal dominant, 2A (DFNA2A)5 Publications
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionA form of non-syndromic sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.
See also OMIM:600101| Feature key | Position(s) | DescriptionActions | Graphical view | Length |
|---|---|---|---|---|
| Natural variantiVAR_010936 | 274 | L → H in DFNA2A. 1 PublicationCorresponds to variant dbSNP:rs80358276EnsemblClinVar. | 1 | |
| Natural variantiVAR_008726 | 276 | W → S in DFNA2A. 1 PublicationCorresponds to variant dbSNP:rs80358277EnsemblClinVar. | 1 | |
| Natural variantiVAR_010937 | 281 | L → S in DFNA2A. 1 PublicationCorresponds to variant dbSNP:rs80358278EnsemblClinVar. | 1 | |
| Natural variantiVAR_008727 | 285 | G → C in DFNA2A; loss of potassium selectivity of the pore. 1 PublicationCorresponds to variant dbSNP:rs28937588EnsemblClinVar. | 1 | |
| Natural variantiVAR_001547 | 285 | G → S in DFNA2A; dominant negative effect; abolishes potassium current. 1 PublicationCorresponds to variant dbSNP:rs28937588EnsemblClinVar. | 1 | |
| Natural variantiVAR_065779 | 287 | G → R in DFNA2A. 1 PublicationCorresponds to variant dbSNP:rs137853969EnsemblClinVar. | 1 | |
| Natural variantiVAR_008728 | 321 | G → S in DFNA2A. 1 PublicationCorresponds to variant dbSNP:rs28939710EnsemblClinVar. | 1 |
Mutagenesis
| Feature key | Position(s) | DescriptionActions | Graphical view | Length |
|---|---|---|---|---|
| Mutagenesisi | 123 | E → G: No effect on inhibition by potassium channel toxin SsTX. 1 Publication | 1 | |
| Mutagenesisi | 124 | H → G: No effect on inhibition by potassium channel toxin SsTX. 1 Publication | 1 | |
| Mutagenesisi | 125 | Q → G: No effect on inhibition by potassium channel toxin SsTX. 1 Publication | 1 | |
| Mutagenesisi | 126 | E → G: No effect on inhibition by potassium channel toxin SsTX. 1 Publication | 1 | |
| Mutagenesisi | 194 | Q → G: No effect on inhibition by potassium channel toxin SsTX. 1 Publication | 1 | |
| Mutagenesisi | 196 | N → Q: No effect on inhibition by potassium channel toxin SsTX. 1 Publication | 1 | |
| Mutagenesisi | 205 | S → G: No effect on inhibition by potassium channel toxin SsTX. 1 Publication | 1 | |
| Mutagenesisi | 210 | Q → G: No effect on inhibition by potassium channel toxin SsTX. 1 Publication | 1 | |
| Mutagenesisi | 213 | R → G: No effect on inhibition by potassium channel toxin SsTX. 1 Publication | 1 | |
| Mutagenesisi | 216 | R → G: No effect on inhibition by potassium channel toxin SsTX. 1 Publication | 1 | |
| Mutagenesisi | 261 | K → G: No effect on inhibition by potassium channel toxin SsTX. 1 Publication | 1 | |
| Mutagenesisi | 262 | D → G: No effect on inhibition by potassium channel toxin SsTX. 1 Publication | 1 | |
| Mutagenesisi | 266 | D → G: Resistant to inhibition by potassium channel toxin SsTX. Normal voltage activation. 1 Publication | 1 | |
| Mutagenesisi | 268 | S → G: No effect on inhibition by potassium channel toxin SsTX. 1 Publication | 1 | |
| Mutagenesisi | 272 | D → G: No effect on inhibition by potassium channel toxin SsTX. 1 Publication | 1 | |
| Mutagenesisi | 288 | D → G: Resistant to inhibition by potassium channel toxin SsTX. Normal voltage activation. 1 Publication | 1 | |
| Mutagenesisi | 290 | T → V: No effect on inhibition by potassium channel toxin SsTX. 1 Publication | 1 | |
| Mutagenesisi | 292 | H → G: No effect on inhibition by potassium channel toxin SsTX. 1 Publication | 1 | |
| Mutagenesisi | 295 | L → V: No effect on inhibition by potassium channel toxin SsTX. 1 Publication | 1 | |
| Mutagenesisi | 298 | V → T: No effect on inhibition by potassium channel toxin SsTX. 1 Publication | 1 |
Keywords - Diseasei
Deafness, Disease mutation, Non-syndromic deafnessOrganism-specific databases
| DisGeNETi | 9132 |
| GeneReviewsi | KCNQ4 |
| MalaCardsi | KCNQ4 |
| MIMi | 600101 phenotype |
| OpenTargetsi | ENSG00000117013 |
| Orphaneti | 90635 Autosomal dominant non-syndromic sensorineural deafness type DFNA |
| PharmGKBi | PA30076 |
Chemistry databases
| ChEMBLi | CHEMBL3576 |
| DrugBanki | DB04953 Ezogabine DB06089 ICA-105665 |
| GuidetoPHARMACOLOGYi | 563 |
Polymorphism and mutation databases
| BioMutai | KCNQ4 |
| DMDMi | 259016259 |
PTM / Processingi
Molecule processing
| Feature key | Position(s) | DescriptionActions | Graphical view | Length |
|---|---|---|---|---|
| ChainiPRO_0000054037 | 1 – 695 | Potassium voltage-gated channel subfamily KQT member 4Add BLAST | 695 |
Proteomic databases
| PaxDbi | P56696 |
| PeptideAtlasi | P56696 |
| PRIDEi | P56696 |
| ProteomicsDBi | 56934 56935 [P56696-2] |
PTM databases
| iPTMneti | P56696 |
| PhosphoSitePlusi | P56696 |
Expressioni
Tissue specificityi
Expressed in the outer, but not the inner, sensory hair cells of the cochlea. Slightly expressed in heart, brain and skeletal muscle.
Gene expression databases
| Bgeei | ENSG00000117013 Expressed in 128 organ(s), highest expression level in pigmented layer of retina |
| CleanExi | HS_KCNQ4 |
| ExpressionAtlasi | P56696 baseline and differential |
| Genevisiblei | P56696 HS |
Organism-specific databases
| HPAi | HPA018305 |
Interactioni
Subunit structurei
Homotetramer. May form heteromultimers with KCNQ3. Interacts with HSP90AB1; promotes cell surface expression of KCNQ4 (PubMed:23431407).2 Publications
Protein-protein interaction databases
| BioGridi | 114580, 6 interactors |
| STRINGi | 9606.ENSP00000262916 |
Chemistry databases
| BindingDBi | P56696 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
| ProteinModelPortali | P56696 |
| SMRi | P56696 |
| ModBasei | Search... |
| MobiDBi | Search... |
Miscellaneous databases
| EvolutionaryTracei | P56696 |
Family & Domainsi
Region
| Feature key | Position(s) | DescriptionActions | Graphical view | Length |
|---|---|---|---|---|
| Regioni | 546 – 650 | A-domain (Tetramerization)Add BLAST | 105 |
Coiled coil
| Feature key | Position(s) | DescriptionActions | Graphical view | Length |
|---|---|---|---|---|
| Coiled coili | 610 – 645 | Add BLAST | 36 |
Motif
| Feature key | Position(s) | DescriptionActions | Graphical view | Length |
|---|---|---|---|---|
| Motifi | 283 – 288 | Selectivity filterBy similarity | 6 |
Domaini
The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.By similarity
The A-domain tail carries the major determinants of channel assembly specificity. Its coiled-coil region is Four-stranded.1 Publication
Sequence similaritiesi
Belongs to the potassium channel family. KQT (TC 1.A.1.15) subfamily. Kv7.4/KCNQ4 sub-subfamily. [View classification]Curated
Keywords - Domaini
Coiled coil, Transmembrane, Transmembrane helixPhylogenomic databases
| eggNOGi | KOG1419 Eukaryota COG1226 LUCA |
| GeneTreei | ENSGT00550000074513 |
| HOGENOMi | HOG000220839 |
| HOVERGENi | HBG059014 |
| InParanoidi | P56696 |
| KOi | K04929 |
| OMAi | WAFVYHV |
| OrthoDBi | EOG091G02ZT |
| PhylomeDBi | P56696 |
| TreeFami | TF315186 |
Family and domain databases
| InterProi | View protein in InterPro IPR005821 Ion_trans_dom IPR003937 K_chnl_volt-dep_KCNQ IPR013821 K_chnl_volt-dep_KCNQ_C IPR015573 KCQN4 IPR028325 VG_K_chnl |
| PANTHERi | PTHR11537 PTHR11537, 1 hit PTHR11537:SF4 PTHR11537:SF4, 1 hit |
| Pfami | View protein in Pfam PF00520 Ion_trans, 1 hit PF03520 KCNQ_channel, 1 hit |
| PRINTSi | PR00169 KCHANNEL PR01459 KCNQCHANNEL |
Sequences (2+)i
Sequence statusi: Complete.
This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basketNote: Additional isoforms seem to exist.
This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.iShow all
Isoform 1 (identifier: P56696-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
10 20 30 40 50
MAEAPPRRLG LGPPPGDAPR AELVALTAVQ SEQGEAGGGG SPRRLGLLGS
60 70 80 90 100
PLPPGAPLPG PGSGSGSACG QRSSAAHKRY RRLQNWVYNV LERPRGWAFV
110 120 130 140 150
YHVFIFLLVF SCLVLSVLST IQEHQELANE CLLILEFVMI VVFGLEYIVR
160 170 180 190 200
VWSAGCCCRY RGWQGRFRFA RKPFCVIDFI VFVASVAVIA AGTQGNIFAT
210 220 230 240 250
SALRSMRFLQ ILRMVRMDRR GGTWKLLGSV VYAHSKELIT AWYIGFLVLI
260 270 280 290 300
FASFLVYLAE KDANSDFSSY ADSLWWGTIT LTTIGYGDKT PHTWLGRVLA
310 320 330 340 350
AGFALLGISF FALPAGILGS GFALKVQEQH RQKHFEKRRM PAANLIQAAW
360 370 380 390 400
RLYSTDMSRA YLTATWYYYD SILPSFRELA LLFEHVQRAR NGGLRPLEVR
410 420 430 440 450
RAPVPDGAPS RYPPVATCHR PGSTSFCPGE SSRMGIKDRI RMGSSQRRTG
460 470 480 490 500
PSKQHLAPPT MPTSPSSEQV GEATSPTKVQ KSWSFNDRTR FRASLRLKPR
510 520 530 540 550
TSAEDAPSEE VAEEKSYQCE LTVDDIMPAV KTVIRSIRIL KFLVAKRKFK
560 570 580 590 600
ETLRPYDVKD VIEQYSAGHL DMLGRIKSLQ TRVDQIVGRG PGDRKAREKG
610 620 630 640 650
DKGPSDAEVV DEISMMGRVV KVEKQVQSIE HKLDLLLGFY SRCLRSGTSA
660 670 680 690
SLGAVQVPLF DPDITSDYHS PVDHEDISVS AQTLSISRSV STNMD
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket| H0Y6N7 | H0Y6N7_HUMAN | Potassium voltage-gated channel sub... | KCNQ4 | 556 | Annotation score: |
Natural variant
| Feature key | Position(s) | DescriptionActions | Graphical view | Length |
|---|---|---|---|---|
| Natural variantiVAR_010936 | 274 | L → H in DFNA2A. 1 PublicationCorresponds to variant dbSNP:rs80358276EnsemblClinVar. | 1 | |
| Natural variantiVAR_008726 | 276 | W → S in DFNA2A. 1 PublicationCorresponds to variant dbSNP:rs80358277EnsemblClinVar. | 1 | |
| Natural variantiVAR_010937 | 281 | L → S in DFNA2A. 1 PublicationCorresponds to variant dbSNP:rs80358278EnsemblClinVar. | 1 | |
| Natural variantiVAR_008727 | 285 | G → C in DFNA2A; loss of potassium selectivity of the pore. 1 PublicationCorresponds to variant dbSNP:rs28937588EnsemblClinVar. | 1 | |
| Natural variantiVAR_001547 | 285 | G → S in DFNA2A; dominant negative effect; abolishes potassium current. 1 PublicationCorresponds to variant dbSNP:rs28937588EnsemblClinVar. | 1 | |
| Natural variantiVAR_065779 | 287 | G → R in DFNA2A. 1 PublicationCorresponds to variant dbSNP:rs137853969EnsemblClinVar. | 1 | |
| Natural variantiVAR_008728 | 321 | G → S in DFNA2A. 1 PublicationCorresponds to variant dbSNP:rs28939710EnsemblClinVar. | 1 | |
| Natural variantiVAR_058971 | 455 | H → Q1 PublicationCorresponds to variant dbSNP:rs34287852EnsemblClinVar. | 1 |
Alternative sequence
| Feature key | Position(s) | DescriptionActions | Graphical view | Length |
|---|---|---|---|---|
| Alternative sequenceiVSP_001013 | 377 – 430 | Missing in isoform 2. 1 PublicationAdd BLAST | 54 |
Sequence databases
| Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF105202 mRNA Translation: AAD14680.1 AF105216 AF105215 Genomic DNA Translation: AAD14681.1 AC119677 Genomic DNA No translation available. |
| CCDSi | CCDS456.1 [P56696-1] |
| RefSeqi | NP_004691.2, NM_004700.3 [P56696-1] NP_751895.1, NM_172163.2 [P56696-2] |
| UniGenei | Hs.473058 |
Genome annotation databases
| Ensembli | ENST00000347132; ENSP00000262916; ENSG00000117013 [P56696-1] ENST00000509682; ENSP00000423756; ENSG00000117013 [P56696-2] ENST00000642618; ENSP00000494128; ENSG00000117013 [P56696-1] |
| GeneIDi | 9132 |
| KEGGi | hsa:9132 |
| UCSCi | uc001cgh.2 human [P56696-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Web resourcesi
| Hereditary hearing loss homepage Gene page |
Sequence databases
| Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF105202 mRNA Translation: AAD14680.1 AF105216 AF105215 Genomic DNA Translation: AAD14681.1 AC119677 Genomic DNA No translation available. |
| CCDSi | CCDS456.1 [P56696-1] |
| RefSeqi | NP_004691.2, NM_004700.3 [P56696-1] NP_751895.1, NM_172163.2 [P56696-2] |
| UniGenei | Hs.473058 |
3D structure databases
| Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
| 2OVC | X-ray | 2.07 | A | 610-640 | [»] | |
| 4GOW | X-ray | 2.60 | A | 522-593 | [»] | |
| ProteinModelPortali | P56696 | |||||
| SMRi | P56696 | |||||
| ModBasei | Search... | |||||
| MobiDBi | Search... | |||||
Protein-protein interaction databases
| BioGridi | 114580, 6 interactors |
| STRINGi | 9606.ENSP00000262916 |
Chemistry databases
| BindingDBi | P56696 |
| ChEMBLi | CHEMBL3576 |
| DrugBanki | DB04953 Ezogabine DB06089 ICA-105665 |
| GuidetoPHARMACOLOGYi | 563 |
Protein family/group databases
| TCDBi | 1.A.1.15.4 the voltage-gated ion channel (vic) superfamily |
PTM databases
| iPTMneti | P56696 |
| PhosphoSitePlusi | P56696 |
Polymorphism and mutation databases
| BioMutai | KCNQ4 |
| DMDMi | 259016259 |
Proteomic databases
| PaxDbi | P56696 |
| PeptideAtlasi | P56696 |
| PRIDEi | P56696 |
| ProteomicsDBi | 56934 56935 [P56696-2] |
Protocols and materials databases
| Structural Biology Knowledgebase | Search... |
Genome annotation databases
| Ensembli | ENST00000347132; ENSP00000262916; ENSG00000117013 [P56696-1] ENST00000509682; ENSP00000423756; ENSG00000117013 [P56696-2] ENST00000642618; ENSP00000494128; ENSG00000117013 [P56696-1] |
| GeneIDi | 9132 |
| KEGGi | hsa:9132 |
| UCSCi | uc001cgh.2 human [P56696-1] |
Organism-specific databases
| CTDi | 9132 |
| DisGeNETi | 9132 |
| EuPathDBi | HostDB:ENSG00000117013.14 |
| GeneCardsi | KCNQ4 |
| GeneReviewsi | KCNQ4 |
| H-InvDBi | HIX0200020 |
| HGNCi | HGNC:6298 KCNQ4 |
| HPAi | HPA018305 |
| MalaCardsi | KCNQ4 |
| MIMi | 600101 phenotype 603537 gene |
| neXtProti | NX_P56696 |
| OpenTargetsi | ENSG00000117013 |
| Orphaneti | 90635 Autosomal dominant non-syndromic sensorineural deafness type DFNA |
| PharmGKBi | PA30076 |
| GenAtlasi | Search... |
Phylogenomic databases
| eggNOGi | KOG1419 Eukaryota COG1226 LUCA |
| GeneTreei | ENSGT00550000074513 |
| HOGENOMi | HOG000220839 |
| HOVERGENi | HBG059014 |
| InParanoidi | P56696 |
| KOi | K04929 |
| OMAi | WAFVYHV |
| OrthoDBi | EOG091G02ZT |
| PhylomeDBi | P56696 |
| TreeFami | TF315186 |
Enzyme and pathway databases
| Reactomei | R-HSA-1296072 Voltage gated Potassium channels |
Miscellaneous databases
| ChiTaRSi | KCNQ4 human |
| EvolutionaryTracei | P56696 |
| GeneWikii | KCNQ4 |
| GenomeRNAii | 9132 |
| PROi | PR:P56696 |
| SOURCEi | Search... |
Gene expression databases
| Bgeei | ENSG00000117013 Expressed in 128 organ(s), highest expression level in pigmented layer of retina |
| CleanExi | HS_KCNQ4 |
| ExpressionAtlasi | P56696 baseline and differential |
| Genevisiblei | P56696 HS |
Family and domain databases
| InterProi | View protein in InterPro IPR005821 Ion_trans_dom IPR003937 K_chnl_volt-dep_KCNQ IPR013821 K_chnl_volt-dep_KCNQ_C IPR015573 KCQN4 IPR028325 VG_K_chnl |
| PANTHERi | PTHR11537 PTHR11537, 1 hit PTHR11537:SF4 PTHR11537:SF4, 1 hit |
| Pfami | View protein in Pfam PF00520 Ion_trans, 1 hit PF03520 KCNQ_channel, 1 hit |
| PRINTSi | PR00169 KCHANNEL PR01459 KCNQCHANNEL |
| ProtoNeti | Search... |
Entry informationi
| Entry namei | KCNQ4_HUMAN | |
| Accessioni | P56696Primary (citable) accession number: P56696 Secondary accession number(s): O96025 | |
| Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1999 |
| Last sequence update: | September 22, 2009 | |
| Last modified: | September 12, 2018 | |
| This is version 171 of the entry and version 2 of the sequence. See complete history. | ||
| Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
| Annotation program | Chordata Protein Annotation Program | |
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | |
