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UniProtKB - P56690 (SYI_THET8)
Protein
Isoleucine--tRNA ligase
Gene
ileS
Organism
Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)
Status
Functioni
Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) (By similarity).
By similarityMiscellaneous
The ATP consumption with regard to L-valine is nonproductive and is solely for substrate selection, which demonstrates the high cost of accuracy.
Catalytic activityi
- EC:6.1.1.5
Cofactori
Zn2+2 PublicationsNote: Binds 2 Zn2+ ions per subunit.2 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 46 | Isoleucyl-adenylateCombined sources1 Publication | 1 | |
Binding sitei | 57 | Isoleucyl-adenylateCombined sources1 Publication | 1 | |
Metal bindingi | 181 | Zinc 1Combined sources | 1 | |
Metal bindingi | 184 | Zinc 1Combined sources | 1 | |
Binding sitei | 319 | Valine1 Publication | 1 | |
Binding sitei | 328 | Valine1 Publication | 1 | |
Metal bindingi | 389 | Zinc 1Combined sources | 1 | |
Metal bindingi | 392 | Zinc 1Combined sources | 1 | |
Metal bindingi | 461 | Zinc 2Combined sources | 1 | |
Metal bindingi | 464 | Zinc 2Combined sources | 1 | |
Metal bindingi | 502 | Zinc 2Combined sources | 1 | |
Metal bindingi | 504 | Zinc 2Combined sources | 1 | |
Binding sitei | 550 | Isoleucyl-adenylateCombined sources1 Publication | 1 | |
Binding sitei | 551 | Isoleucyl-adenylate; via amide nitrogenCombined sources1 Publication | 1 | |
Binding sitei | 553 | Isoleucyl-adenylateCombined sources1 Publication | 1 | |
Binding sitei | 554 | Isoleucyl-adenylateCombined sources1 Publication | 1 | |
Binding sitei | 581 | Isoleucyl-adenylateCombined sources1 Publication | 1 | |
Binding sitei | 594 | ATPBy similarity | 1 |
GO - Molecular functioni
- aminoacyl-tRNA editing activity Source: InterPro
- ATP binding Source: UniProtKB-UniRule
- isoleucine-tRNA ligase activity Source: UniProtKB-UniRule
- tRNA binding Source: InterPro
- zinc ion binding Source: UniProtKB-UniRule
GO - Biological processi
- isoleucyl-tRNA aminoacylation Source: UniProtKB-UniRule
Keywordsi
Molecular function | Aminoacyl-tRNA synthetase, Ligase |
Biological process | Protein biosynthesis |
Ligand | ATP-binding, Metal-binding, Nucleotide-binding, Zinc |
Enzyme and pathway databases
BRENDAi | 6.1.1.5, 2305 |
Names & Taxonomyi
Protein namesi | Recommended name: Isoleucine--tRNA ligase (EC:6.1.1.5)Alternative name(s): Isoleucyl-tRNA synthetase Short name: IleRS |
Gene namesi | Name:ileS Ordered Locus Names:TTHA1067 |
Organismi | Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) |
Taxonomic identifieri | 300852 [NCBI] |
Taxonomic lineagei | Bacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 228 | T → A: Has some defects in posttransfer editing activity. 1 Publication | 1 | |
Mutagenesisi | 229 | T → A: Has some defects in posttransfer editing activity. 1 Publication | 1 | |
Mutagenesisi | 230 | T → A: No change in posttransfer editing activity. 1 Publication | 1 | |
Mutagenesisi | 233 | T → A: No change in posttransfer editing activity. 1 Publication | 1 | |
Mutagenesisi | 328 | D → A: Has some defects in posttransfer editing activity. 1 Publication | 1 |
Chemistry databases
ChEMBLi | CHEMBL3879837 |
DrugBanki | DB01755, N-[Isoleucinyl]-N'-[adenosyl]-diaminosufone |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000098567 | 1 – 1043 | Isoleucine--tRNA ligaseAdd BLAST | 1043 |
Interactioni
Subunit structurei
Monomer.
3 PublicationsProtein-protein interaction databases
STRINGi | 300852.55772449 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | P56690 |
SMRi | P56690 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P56690 |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 47 – 57 | 'HIGH' regionAdd BLAST | 11 | |
Motifi | 591 – 595 | 'KMSKS' region | 5 |
Domaini
IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) (By similarity).By similarity
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0060, Bacteria |
HOGENOMi | CLU_001493_1_1_0 |
OMAi | HLGTAWN |
PhylomeDBi | P56690 |
Family and domain databases
CDDi | cd07961, Anticodon_Ia_Ile_ABEc, 1 hit |
Gene3Di | 3.40.50.620, 2 hits |
HAMAPi | MF_02003, Ile_tRNA_synth_type2, 1 hit |
InterProi | View protein in InterPro IPR001412, aa-tRNA-synth_I_CS IPR002300, aa-tRNA-synth_Ia IPR033709, Anticodon_Ile_ABEc IPR002301, Ile-tRNA-ligase IPR023586, Ile-tRNA-ligase_type2 IPR013155, M/V/L/I-tRNA-synth_anticd-bd IPR014729, Rossmann-like_a/b/a_fold IPR009080, tRNAsynth_Ia_anticodon-bd IPR009008, Val/Leu/Ile-tRNA-synth_edit |
PANTHERi | PTHR42780, PTHR42780, 1 hit |
Pfami | View protein in Pfam PF08264, Anticodon_1, 1 hit PF00133, tRNA-synt_1, 1 hit |
PRINTSi | PR00984, TRNASYNTHILE |
SUPFAMi | SSF47323, SSF47323, 1 hit SSF50677, SSF50677, 1 hit |
TIGRFAMsi | TIGR00392, ileS, 1 hit |
PROSITEi | View protein in PROSITE PS00178, AA_TRNA_LIGASE_I, 1 hit |
i Sequence
Sequence statusi: Complete.
P56690-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MFKEVGEPNF PKLEEEVLAF WKREKIFQKS VENRKGGPRY TVYEGPPTAN
60 70 80 90 100
GLPHVGHAQA RSYKDLFPRY KTMRGYYAPR RAGWDTHGLP VELEVEKKLG
110 120 130 140 150
LKSKREIEAY GIERFNQACR ESVFTYEKEW EAFTERIAYW VDLENAYATL
160 170 180 190 200
EPTYIESIWW SLKNLFDRGL LYRDHKVVPY CPRCGTPLSS HEVALGYKEI
210 220 230 240 250
QDPSVYVRFP LKEPKKLGLE KASLLIWTTT PWTLPGNVAA AVHPEYTYAA
260 270 280 290 300
FQVGDEALIL EEGLGRKLLG EGTPVLKTFP GKALEGLPYT PPYPQALEKG
310 320 330 340 350
YFVVLADYVS QEDGTGIVHQ APAFGAEDLE TARVYGLPLL KTVDEEGKLL
360 370 380 390 400
VEPFKGLYFR EANRAILRDL RGRGLLFKEE SYLHSYPHCW RCSTPLMYYA
410 420 430 440 450
TESWFIKNTL FKDELIRKNQ EIHWVPPHIK EGRYGEWLKN LVDWALSRNR
460 470 480 490 500
YWGTPLPIWV CQACGKEEAI GSFQELKARA TKPLPEPFDP HRPYVDQVEL
510 520 530 540 550
ACACGGTMRR VPYVIDVWYD SGAMPFASLH YPFEHEEVFR ESFPADFIAE
560 570 580 590 600
GIDQTRGWFN SLHQLGVMLF GSIAFKNVIC HGLILDEKGQ KMSKSKGNVV
610 620 630 640 650
DPWDIIREFG ADALRWYIYV SAPPEADRRF GPNLVRETVR DYFLTLWNVY
660 670 680 690 700
SFFVTYANLD RPDLKNPPPP EKRPEMDRWL LARMQDLIQR VTEALEAYDP
710 720 730 740 750
TTSARALRDF VVEDLSQWYV RRNRRRFWKN EDALDREAAY ATLYEALVLV
760 770 780 790 800
ATLAAPFTPF LAEVLWQNLV RSVRPEAKES VHLADWPEAD PALADEALVA
810 820 830 840 850
QMRAVLKVVD LARAARAKSG VKTRTPLPLL LVTAPTALER EGLKRFAHEI
860 870 880 890 900
AEELNVKEVR VLEPGEEILS YRVLPNLKLL GRKYGKLVPK IREALQRERE
910 920 930 940 950
RAAALALKGE AIPLEVEGEA LTLLPEEVLL EAEAPKGYQA LEKDGYVAAL
960 970 980 990 1000
KVEVTEALRM EGLARDLIRL LQQARKDMGL KVSDRIRVGY EAEGPYLEAL
1010 1020 1030 1040
KRHGPWIAEE VLATAFGEGL FGGFEARVED EEGKAVFHLA RAE
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AP008226 Genomic DNA Translation: BAD70890.1 |
RefSeqi | WP_011228416.1, NC_006461.1 YP_144333.1, NC_006461.1 |
Genome annotation databases
EnsemblBacteriai | BAD70890; BAD70890; BAD70890 |
GeneIDi | 3168241 |
KEGGi | ttj:TTHA1067 |
PATRICi | fig|300852.9.peg.1047 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AP008226 Genomic DNA Translation: BAD70890.1 |
RefSeqi | WP_011228416.1, NC_006461.1 YP_144333.1, NC_006461.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1ILE | X-ray | 2.50 | A | 1-821 | [»] | |
1JZQ | X-ray | 3.00 | A | 1-821 | [»] | |
1JZS | X-ray | 2.50 | A | 1-821 | [»] | |
1UDZ | X-ray | 1.80 | A/B | 201-381 | [»] | |
1UE0 | X-ray | 2.00 | A/B | 201-381 | [»] | |
1WK8 | X-ray | 1.70 | A/B | 196-388 | [»] | |
1WNY | X-ray | 1.60 | A/B | 201-385 | [»] | |
1WNZ | X-ray | 1.70 | A | 201-385 | [»] | |
AlphaFoldDBi | P56690 | |||||
SMRi | P56690 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 300852.55772449 |
Chemistry databases
ChEMBLi | CHEMBL3879837 |
DrugBanki | DB01755, N-[Isoleucinyl]-N'-[adenosyl]-diaminosufone |
Genome annotation databases
EnsemblBacteriai | BAD70890; BAD70890; BAD70890 |
GeneIDi | 3168241 |
KEGGi | ttj:TTHA1067 |
PATRICi | fig|300852.9.peg.1047 |
Phylogenomic databases
eggNOGi | COG0060, Bacteria |
HOGENOMi | CLU_001493_1_1_0 |
OMAi | HLGTAWN |
PhylomeDBi | P56690 |
Enzyme and pathway databases
BRENDAi | 6.1.1.5, 2305 |
Miscellaneous databases
EvolutionaryTracei | P56690 |
Family and domain databases
CDDi | cd07961, Anticodon_Ia_Ile_ABEc, 1 hit |
Gene3Di | 3.40.50.620, 2 hits |
HAMAPi | MF_02003, Ile_tRNA_synth_type2, 1 hit |
InterProi | View protein in InterPro IPR001412, aa-tRNA-synth_I_CS IPR002300, aa-tRNA-synth_Ia IPR033709, Anticodon_Ile_ABEc IPR002301, Ile-tRNA-ligase IPR023586, Ile-tRNA-ligase_type2 IPR013155, M/V/L/I-tRNA-synth_anticd-bd IPR014729, Rossmann-like_a/b/a_fold IPR009080, tRNAsynth_Ia_anticodon-bd IPR009008, Val/Leu/Ile-tRNA-synth_edit |
PANTHERi | PTHR42780, PTHR42780, 1 hit |
Pfami | View protein in Pfam PF08264, Anticodon_1, 1 hit PF00133, tRNA-synt_1, 1 hit |
PRINTSi | PR00984, TRNASYNTHILE |
SUPFAMi | SSF47323, SSF47323, 1 hit SSF50677, SSF50677, 1 hit |
TIGRFAMsi | TIGR00392, ileS, 1 hit |
PROSITEi | View protein in PROSITE PS00178, AA_TRNA_LIGASE_I, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | SYI_THET8 | |
Accessioni | P56690Primary (citable) accession number: P56690 Secondary accession number(s): Q5SJE7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1999 |
Last sequence update: | March 29, 2005 | |
Last modified: | May 25, 2022 | |
This is version 145 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Aminoacyl-tRNA synthetases
List of aminoacyl-tRNA synthetase entries - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families