UniProtKB - P56677 (ST14_MOUSE)
Protein
Suppressor of tumorigenicity 14 protein homolog
Gene
St14
Organism
Mus musculus (Mouse)
Status
Functioni
Degrades extracellular matrix. Exhibits trypsin-like activity as defined by cleavage of synthetic substrates with Arg or Lys as the P1 site (By similarity). Involved in the terminal differentiation of keratinocytes through prostasin (PRSS8) activation and filaggrin (FLG) processing (By similarity).By similarity
Catalytic activityi
- Cleaves various synthetic substrates with Arg or Lys at the P1 position and prefers small side-chain amino acids, such as Ala and Gly, at the P2 position. EC:3.4.21.109
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 656 | Charge relay systemBy similarity | 1 | |
Active sitei | 711 | Charge relay systemBy similarity | 1 | |
Active sitei | 805 | Charge relay systemBy similarity | 1 |
GO - Molecular functioni
- serine-type endopeptidase activity Source: Reactome
- serine-type peptidase activity Source: MGI
GO - Biological processi
- cell migration Source: MGI
- epithelial cell morphogenesis involved in placental branching Source: MGI
- keratinocyte differentiation Source: UniProtKB
- neural tube closure Source: MGI
- proteolysis Source: MGI
Keywordsi
Molecular function | Hydrolase, Protease, Serine protease |
Enzyme and pathway databases
Reactomei | R-MMU-6809371, Formation of the cornified envelope |
Protein family/group databases
MEROPSi | S01.302 |
Names & Taxonomyi
Protein namesi | Recommended name: Suppressor of tumorigenicity 14 protein homolog (EC:3.4.21.109)Alternative name(s): Epithin Serine protease 14 |
Gene namesi | Name:St14 Synonyms:Prss14 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1338881, St14 |
Subcellular locationi
Other locations
- Membrane Curated; Single-pass type II membrane protein Curated
Extracellular region or secreted
- extracellular region Source: MGI
- extracellular space Source: MGI
Plasma Membrane
- basolateral plasma membrane Source: MGI
- extrinsic component of plasma membrane Source: MGI
- integral component of plasma membrane Source: MGI
- plasma membrane Source: Reactome
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 55 | CytoplasmicSequence analysisAdd BLAST | 55 | |
Transmembranei | 56 – 76 | Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST | 21 | |
Topological domaini | 77 – 855 | ExtracellularSequence analysisAdd BLAST | 779 |
Keywords - Cellular componenti
MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000088713 | 1 – 855 | Suppressor of tumorigenicity 14 protein homologAdd BLAST | 855 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 13 | PhosphoserineCombined sources | 1 | |
Glycosylationi | 107 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 214 ↔ 244 | By similarity | ||
Glycosylationi | 302 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 340 ↔ 366 | By similarity | ||
Glycosylationi | 365 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 397 ↔ 410 | By similarity | ||
Glycosylationi | 421 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Disulfide bondi | 453 ↔ 464 | By similarity | ||
Disulfide bondi | 459 ↔ 477 | By similarity | ||
Disulfide bondi | 471 ↔ 486 | By similarity | ||
Disulfide bondi | 488 ↔ 501 | By similarity | ||
Glycosylationi | 489 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 496 ↔ 514 | By similarity | ||
Disulfide bondi | 508 ↔ 523 | By similarity | ||
Disulfide bondi | 525 ↔ 537 | By similarity | ||
Disulfide bondi | 532 ↔ 550 | By similarity | ||
Disulfide bondi | 544 ↔ 559 | By similarity | ||
Disulfide bondi | 567 ↔ 579 | By similarity | ||
Disulfide bondi | 574 ↔ 593 | By similarity | ||
Disulfide bondi | 587 ↔ 602 | By similarity | ||
Disulfide bondi | 641 ↔ 657 | By similarity | ||
Glycosylationi | 772 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 776 ↔ 790 | By similarity | ||
Disulfide bondi | 801 ↔ 830 | By similarity |
Keywords - PTMi
Disulfide bond, Glycoprotein, PhosphoproteinProteomic databases
EPDi | P56677 |
PaxDbi | P56677 |
PeptideAtlasi | P56677 |
PRIDEi | P56677 |
PTM databases
GlyGeni | P56677, 6 sites |
iPTMneti | P56677 |
PhosphoSitePlusi | P56677 |
Expressioni
Tissue specificityi
Highly expressed in intestine, kidney, lung, and thymus. Not expressed in skeletal muscle, liver, heart, testis and brain.
Gene expression databases
Bgeei | ENSMUSG00000031995, Expressed in hair follicle and 194 other tissues |
ExpressionAtlasi | P56677, baseline and differential |
Genevisiblei | P56677, MM |
Interactioni
Subunit structurei
Interacts with CDCP1. May interact with TMEFF1 (By similarity).
By similarityProtein-protein interaction databases
STRINGi | 10090.ENSMUSP00000034478 |
Chemistry databases
BindingDBi | P56677 |
Miscellaneous databases
RNActi | P56677, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 86 – 203 | SEAPROSITE-ProRule annotationAdd BLAST | 118 | |
Domaini | 214 – 331 | CUB 1PROSITE-ProRule annotationAdd BLAST | 118 | |
Domaini | 340 – 444 | CUB 2PROSITE-ProRule annotationAdd BLAST | 105 | |
Domaini | 451 – 488 | LDL-receptor class A 1PROSITE-ProRule annotationAdd BLAST | 38 | |
Domaini | 489 – 522 | LDL-receptor class A 2PROSITE-ProRule annotationAdd BLAST | 34 | |
Domaini | 523 – 561 | LDL-receptor class A 3PROSITE-ProRule annotationAdd BLAST | 39 | |
Domaini | 565 – 604 | LDL-receptor class A 4PROSITE-ProRule annotationAdd BLAST | 40 | |
Domaini | 615 – 854 | Peptidase S1PROSITE-ProRule annotationAdd BLAST | 240 |
Sequence similaritiesi
Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Keywords - Domaini
Repeat, Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3627, Eukaryota |
GeneTreei | ENSGT00940000155418 |
HOGENOMi | CLU_006842_19_3_1 |
InParanoidi | P56677 |
OMAi | MFWKCDG |
OrthoDBi | 1314811at2759 |
PhylomeDBi | P56677 |
TreeFami | TF330647 |
Family and domain databases
CDDi | cd00041, CUB, 2 hits cd00112, LDLa, 4 hits cd00190, Tryp_SPc, 1 hit |
Gene3Di | 2.40.10.10, 2 hits 2.60.120.290, 2 hits 3.30.70.960, 1 hit 4.10.400.10, 4 hits |
InterProi | View protein in InterPro IPR000859, CUB_dom IPR036055, LDL_receptor-like_sf IPR023415, LDLR_class-A_CS IPR002172, LDrepeatLR_classA_rpt IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR017051, Peptidase_S1A_matripase IPR000082, SEA_dom IPR036364, SEA_dom_sf IPR035914, Sperma_CUB_dom_sf IPR001254, Trypsin_dom IPR018114, TRYPSIN_HIS IPR033116, TRYPSIN_SER |
Pfami | View protein in Pfam PF00431, CUB, 2 hits PF00057, Ldl_recept_a, 4 hits PF01390, SEA, 1 hit PF00089, Trypsin, 1 hit |
PIRSFi | PIRSF036370, ST14, 1 hit |
PRINTSi | PR00261, LDLRECEPTOR |
SMARTi | View protein in SMART SM00042, CUB, 2 hits SM00192, LDLa, 4 hits SM00020, Tryp_SPc, 1 hit |
SUPFAMi | SSF49854, SSF49854, 2 hits SSF50494, SSF50494, 1 hit SSF57424, SSF57424, 4 hits SSF82671, SSF82671, 1 hit |
PROSITEi | View protein in PROSITE PS01180, CUB, 2 hits PS01209, LDLRA_1, 3 hits PS50068, LDLRA_2, 4 hits PS50024, SEA, 1 hit PS50240, TRYPSIN_DOM, 1 hit PS00134, TRYPSIN_HIS, 1 hit PS00135, TRYPSIN_SER, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
P56677-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGSNRGRKAG GGSQDFGAGL KYNSRLENMN GFEEGVEFLP ANNAKKVEKR
60 70 80 90 100
GPRRWVVLVA VLFSFLLLSL MAGLLVWHFH YRNVRVQKVF NGHLRITNEI
110 120 130 140 150
FLDAYENSTS TEFISLASQV KEALKLLYNE VPVLGPYHKK SAVTAFSEGS
160 170 180 190 200
VIAYYWSEFS IPPHLAEEVD RAMAVERVVT LPPRARALKS FVLTSVVAFP
210 220 230 240 250
IDPRMLQRTQ DNSCSFALHA HGAAVTRFTT PGFPNSPYPA HARCQWVLRG
260 270 280 290 300
DADSVLSLTF RSFDVAPCDE HGSDLVTVYD SLSPMEPHAV VRLCGTFSPS
310 320 330 340 350
YNLTFLSSQN VFLVTLITNT DRRHPGFEAT FFQLPKMSSC GGFLSDTQGT
360 370 380 390 400
FSSPYYPGHY PPNINCTWNI KVPNNRNVKV RFKLFYLVDP NVPVGSCTKD
410 420 430 440 450
YVEINGEKYC GERSQFVVSS NSSKITVHFH SDHSYTDTGF LAEYLSYDSN
460 470 480 490 500
DPCPGMFMCK TGRCIRKELR CDGWADCPDY SDERYCRCNA THQFTCKNQF
510 520 530 540 550
CKPLFWVCDS VNDCGDGSDE EGCSCPAGSF KCSNGKCLPQ SQKCNGKDNC
560 570 580 590 600
GDGSDEASCD SVNVVSCTKY TYRCQNGLCL SKGNPECDGK TDCSDGSDEK
610 620 630 640 650
NCDCGLRSFT KQARVVGGTN ADEGEWPWQV SLHALGQGHL CGASLISPDW
660 670 680 690 700
LVSAAHCFQD DKNFKYSDYT MWTAFLGLLD QSKRSASGVQ ELKLKRIITH
710 720 730 740 750
PSFNDFTFDY DIALLELEKS VEYSTVVRPI CLPDATHVFP AGKAIWVTGW
760 770 780 790 800
GHTKEGGTGA LILQKGEIRV INQTTCEDLM PQQITPRMMC VGFLSGGVDS
810 820 830 840 850
CQGDSGGPLS SAEKDGRMFQ AGVVSWGEGC AQRNKPGVYT RLPVVRDWIK
EHTGV
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketD6RIN3 | D6RIN3_MOUSE | Suppressor of tumorigenicity 14 pro... | St14 | 43 | Annotation score: | ||
A0A1L1SU89 | A0A1L1SU89_MOUSE | Suppressor of tumorigenicity 14 pro... | St14 | 40 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF042822 mRNA Translation: AAD02230.3 BC005496 mRNA Translation: AAH05496.1 |
CCDSi | CCDS22948.1 |
RefSeqi | NP_035306.2, NM_011176.4 |
Genome annotation databases
Ensembli | ENSMUST00000034478; ENSMUSP00000034478; ENSMUSG00000031995 |
GeneIDi | 19143 |
KEGGi | mmu:19143 |
UCSCi | uc009ori.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF042822 mRNA Translation: AAD02230.3 BC005496 mRNA Translation: AAH05496.1 |
CCDSi | CCDS22948.1 |
RefSeqi | NP_035306.2, NM_011176.4 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000034478 |
Chemistry databases
BindingDBi | P56677 |
ChEMBLi | CHEMBL3745587 |
Protein family/group databases
MEROPSi | S01.302 |
PTM databases
GlyGeni | P56677, 6 sites |
iPTMneti | P56677 |
PhosphoSitePlusi | P56677 |
Proteomic databases
EPDi | P56677 |
PaxDbi | P56677 |
PeptideAtlasi | P56677 |
PRIDEi | P56677 |
Protocols and materials databases
Antibodypediai | 33087, 406 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000034478; ENSMUSP00000034478; ENSMUSG00000031995 |
GeneIDi | 19143 |
KEGGi | mmu:19143 |
UCSCi | uc009ori.1, mouse |
Organism-specific databases
CTDi | 6768 |
MGIi | MGI:1338881, St14 |
Phylogenomic databases
eggNOGi | KOG3627, Eukaryota |
GeneTreei | ENSGT00940000155418 |
HOGENOMi | CLU_006842_19_3_1 |
InParanoidi | P56677 |
OMAi | MFWKCDG |
OrthoDBi | 1314811at2759 |
PhylomeDBi | P56677 |
TreeFami | TF330647 |
Enzyme and pathway databases
Reactomei | R-MMU-6809371, Formation of the cornified envelope |
Miscellaneous databases
BioGRID-ORCSi | 19143, 0 hits in 17 CRISPR screens |
ChiTaRSi | St14, mouse |
PROi | PR:P56677 |
RNActi | P56677, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000031995, Expressed in hair follicle and 194 other tissues |
ExpressionAtlasi | P56677, baseline and differential |
Genevisiblei | P56677, MM |
Family and domain databases
CDDi | cd00041, CUB, 2 hits cd00112, LDLa, 4 hits cd00190, Tryp_SPc, 1 hit |
Gene3Di | 2.40.10.10, 2 hits 2.60.120.290, 2 hits 3.30.70.960, 1 hit 4.10.400.10, 4 hits |
InterProi | View protein in InterPro IPR000859, CUB_dom IPR036055, LDL_receptor-like_sf IPR023415, LDLR_class-A_CS IPR002172, LDrepeatLR_classA_rpt IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR017051, Peptidase_S1A_matripase IPR000082, SEA_dom IPR036364, SEA_dom_sf IPR035914, Sperma_CUB_dom_sf IPR001254, Trypsin_dom IPR018114, TRYPSIN_HIS IPR033116, TRYPSIN_SER |
Pfami | View protein in Pfam PF00431, CUB, 2 hits PF00057, Ldl_recept_a, 4 hits PF01390, SEA, 1 hit PF00089, Trypsin, 1 hit |
PIRSFi | PIRSF036370, ST14, 1 hit |
PRINTSi | PR00261, LDLRECEPTOR |
SMARTi | View protein in SMART SM00042, CUB, 2 hits SM00192, LDLa, 4 hits SM00020, Tryp_SPc, 1 hit |
SUPFAMi | SSF49854, SSF49854, 2 hits SSF50494, SSF50494, 1 hit SSF57424, SSF57424, 4 hits SSF82671, SSF82671, 1 hit |
PROSITEi | View protein in PROSITE PS01180, CUB, 2 hits PS01209, LDLRA_1, 3 hits PS50068, LDLRA_2, 4 hits PS50024, SEA, 1 hit PS50240, TRYPSIN_DOM, 1 hit PS00134, TRYPSIN_HIS, 1 hit PS00135, TRYPSIN_SER, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ST14_MOUSE | |
Accessioni | P56677Primary (citable) accession number: P56677 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1999 |
Last sequence update: | May 4, 2001 | |
Last modified: | December 2, 2020 | |
This is version 184 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - Peptidase families
Classification of peptidase families and list of entries