UniProtKB - P56658 (ADA_BOVIN)

BLAST

>sp|P56658|ADA_BOVIN Adenosine deaminase OS=Bos taurus OX=9913 GN=ADA PE=1 SV=3
MAQTPAFNKPKVELHVHLDGAIKPETILYYGRKRGIALPADTPEELQNIIGMDKPLSLPE
FLAKFDYYMPAIAGCREAVKRIAYEFVEMKAKDGVVYVEVRYSPHLLANSKVEPIPWNQA
EGDLTPDEVVSLVNQGLQEGERDFGVKVRSILCCMRHQPSWSSEVVELCKKYREQTVVAI
DLAGDETIEGSSLFPGHVKAYAEAVKSGVHRTVHAGEVGSANVVKEAVDTLKTERLGHGY
HTLEDATLYNRLRQENMHFEVCPWSSYLTGAWKPDTEHPVVRFKNDQVNYSLNTDDPLIF
KSTLDTDYQMTKNEMGFTEEEFKRLNINAAKSSFLPEDEKKELLDLLYKAYGMPSPASAE
QCL

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History
Entry version 131 (28 Feb 2018)
Sequence version 3 (23 Jan 2007)
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Protein

Adenosine deaminase

Gene

ADA

Organism

Bos taurus (Bovine)

Status

Reviewed-Annotation score: -Experimental evidence at protein levelⁱ

Functionⁱ

Catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine (By similarity). Plays an important role in purine metabolism and in adenosine homeostasis (By similarity). Modulates signaling by extracellular adenosine, and so contributes indirectly to cellular signaling events (By similarity). Acts as a positive regulator of T-cell coactivation, by binding DPP4 (By similarity). Its interaction with DPP4 regulates lymphocyte-epithelial cell adhesion (By similarity). Enhances dendritic cell immunogenicity by affecting dendritic cell costimulatory molecule expression and cytokines and chemokines secretion (PubMed:23240012). Enhances CD4+ T-cell differentiation and proliferation (By similarity). Acts as a positive modulator of adenosine receptors ADORA1 and ADORA2A, by enhancing their ligand affinity via conformational change (By similarity). Stimulates plasminogen activation (By similarity). Plays a role in male fertility (By similarity). Plays a protective role in early postimplantation embryonic development (By similarity).By similarity1 Publication

Catalytic activityⁱ

Adenosine + H₂O = inosine + NH₃.By similarity

Cofactorⁱ

Zn²⁺4 PublicationsNote: Binds 1 zinc ion per subunit.1 Publication

Sites

Feature key	Position(s)	DescriptionActions	Length
Metal bindingⁱ	15	Zinc; catalyticCombined sources9 Publications	1
Metal bindingⁱ	17	Zinc; catalyticCombined sources9 Publications	1
Binding siteⁱ	17	SubstrateCombined sources6 Publications	1
Binding siteⁱ	19	SubstrateCombined sources7 Publications	1
Siteⁱ	58	Important for interaction with adenosine receptors and increasing their affinity for agonistsBy similarity	1
Siteⁱ	62	Important for interaction with adenosine receptors and increasing their affinity for agonistsBy similarity	1
Binding siteⁱ	184	Substrate; via amide nitrogen and carbonyl oxygenCombined sources2 Publications	1
Metal bindingⁱ	214	Zinc; catalyticCombined sources9 Publications	1
Active siteⁱ	217	Proton donorBy similarity	1
Siteⁱ	238	Important for catalytic activityBy similarity	1
Metal bindingⁱ	295	Zinc; catalyticCombined sources9 Publications	1
Binding siteⁱ	296	SubstrateCombined sources6 Publications	1

GO - Molecular functionⁱ

adenosine deaminase activity Source: UniProtKB
zinc ion binding Source: UniProtKB

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

adenosine catabolic process Source: UniProtKB
cell adhesion Source: UniProtKB-KW
hypoxanthine salvage
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
inosine biosynthetic process Source: UniProtKB
negative regulation of adenosine receptor signaling pathway
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
nucleotide metabolic process Source: UniProtKB-KW
purine ribonucleoside monophosphate biosynthetic process Source: InterPro
T cell activation
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Hydrolase
Biological process	Cell adhesion, Nucleotide metabolism
Ligand	Metal-binding, Zinc

Enzyme and pathway databases

BRENDAⁱ	3.5.4.4 908

BRENDAⁱ

3.5.4.4 908

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Adenosine deaminase (EC:3.5.4.4By similarity) Alternative name(s): Adenosine aminohydrolase
Gene namesⁱ	Name:ADA
Organismⁱ	Bos taurus (Bovine)
Taxonomic identifierⁱ	9913 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos
Proteomesⁱ	UP000009136 Componentⁱ: Unplaced

Keywords - Cellular componentⁱ

Cell junction, Cell membrane, Cytoplasm, Cytoplasmic vesicle, Lysosome, Membrane

Pathology & Biotechⁱ

Pharmaceutical useⁱ

Available under the name Adagen (Enzon). This is a PEG-conjugated form (pegademase). Used to treat patients with severe combined immunodeficiency diseases (SCID).

Chemistry databases

ChEMBLⁱ	CHEMBL2966

ChEMBLⁱ

CHEMBL2966

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Initiator methionineⁱ		RemovedBy similarity1 Publication
ChainⁱPRO_0000194351	2 – 363	Adenosine deaminaseAdd BLAST		362

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	2	N-acetylalanineBy similarity	1
Modified residueⁱ	54	N6-acetyllysineBy similarity	1
Modified residueⁱ	232	N6-acetyllysineBy similarity	1

Keywords - PTMⁱ

Acetylation

Proteomic databases

PaxDbⁱ	P56658
PeptideAtlas More...PeptideAtlasⁱ	P56658
PRIDEⁱ	P56658

Expressionⁱ

Tissue specificityⁱ

Expressed in gastrointestinal tissues (at protein level).1 Publication

Interactionⁱ

Subunit structureⁱ

Interacts with DPP4 (via extracellular domain). Interacts with PLG (via Kringle 4 domain); the interaction stimulates PLG activation when in complex with DPP4.By similarity

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
DPP4	P27487	5	EBI-7475530,EBI-2871277	From Homo sapiens.

With

Entry

#Exp.

IntAct

Notes

DPP4

P27487

EBI-7475530,EBI-2871277

From Homo sapiens.

Protein-protein interaction databases

IntActⁱ	P56658, 1 interactor
Molecular INTeraction database More...MINTⁱ	P56658
STRINGⁱ	9913.ENSBTAP00000006947

Chemistry databases

BindingDBⁱ

P56658

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Beta strandⁱ	11 – 13	Combined sources	3
Helixⁱ	18 – 20	Combined sources	3
Helixⁱ	24 – 33	Combined sources	10
Helixⁱ	43 – 50	Combined sources	8
Helixⁱ	58 – 72	Combined sources	15
Helixⁱ	76 – 92	Combined sources	17
Beta strandⁱ	95 – 102	Combined sources	8
Helixⁱ	105 – 107	Combined sources	3
Beta strandⁱ	109 – 111	Combined sources	3
Helixⁱ	116 – 118	Combined sources	3
Helixⁱ	126 – 144	Combined sources	19
Beta strandⁱ	147 – 155	Combined sources	9
Helixⁱ	159 – 161	Combined sources	3
Helixⁱ	162 – 171	Combined sources	10
Turnⁱ	174 – 176	Combined sources	3
Beta strandⁱ	177 – 184	Combined sources	8
Helixⁱ	191 – 193	Combined sources	3
Helixⁱ	195 – 207	Combined sources	13
Beta strandⁱ	210 – 219	Combined sources	10
Helixⁱ	221 – 229	Combined sources	9
Beta strandⁱ	234 – 238	Combined sources	5
Helixⁱ	240 – 244	Combined sources	5
Helixⁱ	246 – 254	Combined sources	9
Beta strandⁱ	258 – 261	Combined sources	4
Helixⁱ	263 – 269	Combined sources	7
Beta strandⁱ	270 – 272	Combined sources	3
Helixⁱ	279 – 285	Combined sources	7
Beta strandⁱ	289 – 292	Combined sources	4
Helixⁱ	297 – 300	Combined sources	4
Helixⁱ	304 – 313	Combined sources	10
Helixⁱ	319 – 331	Combined sources	13
Beta strandⁱ	333 – 335	Combined sources	3
Helixⁱ	337 – 351	Combined sources	15

Show more details

3D structure databases

ProteinModelPortalⁱ	P56658
SMRⁱ	P56658
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ

P56658

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the metallo-dependent hydrolases superfamily. Adenosine and AMP deaminases family.Curated

Phylogenomic databases

eggNOGⁱ	KOG1097 Eukaryota COG1816 LUCA
HOGENOMⁱ	HOG000218816
HOVERGENⁱ	HBG001718
InParanoidⁱ	P56658
KEGG Orthology (KO) More...KOⁱ	K01488

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd01320 ADA, 1 hit
HAMAPⁱ	MF_00540 A_deaminase, 1 hit
InterProⁱ	View protein in InterPro IPR006650 A/AMP_deam_AS IPR001365 A/AMP_deaminase_dom IPR028893 A_deaminase IPR006330 Ado/ade_deaminase IPR032466 Metal_Hydrolase
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00962 A_deaminase, 1 hit
SUPFAMⁱ	SSF51556 SSF51556, 1 hit
TIGRFAMsⁱ	TIGR01430 aden_deam, 1 hit
PROSITEⁱ	View protein in PROSITE PS00485 A_DEAMINASE, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

P56658-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MAQTPAFNKP KVELHVHLDG AIKPETILYY GRKRGIALPA DTPEELQNII 
        60         70         80         90        100
GMDKPLSLPE FLAKFDYYMP AIAGCREAVK RIAYEFVEMK AKDGVVYVEV 
       110        120        130        140        150
RYSPHLLANS KVEPIPWNQA EGDLTPDEVV SLVNQGLQEG ERDFGVKVRS 
       160        170        180        190        200
ILCCMRHQPS WSSEVVELCK KYREQTVVAI DLAGDETIEG SSLFPGHVKA 
       210        220        230        240        250
YAEAVKSGVH RTVHAGEVGS ANVVKEAVDT LKTERLGHGY HTLEDATLYN 
       260        270        280        290        300
RLRQENMHFE VCPWSSYLTG AWKPDTEHPV VRFKNDQVNY SLNTDDPLIF 
       310        320        330        340        350
KSTLDTDYQM TKNEMGFTEE EFKRLNINAA KSSFLPEDEK KELLDLLYKA 
       360 
YGMPSPASAE QCL

Length:363

Mass (Da):40,919

Last modified:January 23, 2007 - v3

Checksum:ⁱ69F6D25ACFF2A3E5

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	8	N → D AA sequence (PubMed:8877857).Curated	1
Sequence conflictⁱ	32 – 33	RK → KR AA sequence (PubMed:8877857).Curated	2
Sequence conflictⁱ	57	S → T AA sequence (PubMed:8877857).Curated	1
Sequence conflictⁱ	60	E → D AA sequence (PubMed:8877857).Curated	1
Sequence conflictⁱ	77 – 79	EAV → DAI AA sequence (PubMed:8877857).Curated	3
Sequence conflictⁱ	261	V → I AA sequence (PubMed:8877857).Curated	1
Sequence conflictⁱ	279 – 281	PVV → AVI AA sequence (PubMed:8877857).Curated	3
Sequence conflictⁱ	313 – 314	NE → KD AA sequence (PubMed:8877857).Curated	2

Mass spectrometryⁱ

Molecular mass is 40549±16 Da from positions 2 - 363. Determined by ESI. 1 Publication

Natural variant

Feature key	Position(s)	DescriptionActions	Length
Natural variantⁱ	199	K → Q.	1
Natural variantⁱ	246	A → T.	1
Natural variantⁱ	352	G → R.	1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF280603 mRNA Translation: AAF91430.1
NCBI Reference Sequences More...RefSeqⁱ	NP_776312.1, NM_173887.2
UniGeneⁱ	Bt.4303

Genome annotation databases

GeneIDⁱ	280712
KEGGⁱ	bta:280712

Keywords - Coding sequence diversityⁱ

Polymorphism

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
P56658	Adenosine deaminase		BOVIN		363	UniRef90_P56658
UPI00095213D4		BOSIN		363
UPI0005770834		Bison bison bison		363
UPI0003C0114A		wild yak		312
UPI000D53267F		BOVIN		384
+2

Protein	Similar proteins		Species	Score	Length	Source
P56658	Adenosine deaminase	)	HUMAN		363	UniRef50_P00813
Adenosine deaminase		XENLA		358
Adenosine deaminase		XENTR		358
Uncharacterized protein		MACNE		449
Uncharacterized protein		CERAT		449
+342

Cross-referencesⁱ

Web resourcesⁱ

Worthington enzyme manual

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF280603 mRNA Translation: AAF91430.1
RefSeqⁱ	NP_776312.1, NM_173887.2
UniGeneⁱ	Bt.4303

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	1KRM	X-ray	2.50	A	2-357	[»]
	1NDV	X-ray	2.30	A	2-357	[»]
	1NDW	X-ray	2.00	A	2-357	[»]
	1NDY	X-ray	2.00	A	2-357	[»]
	1NDZ	X-ray	2.00	A	2-357	[»]
	1O5R	X-ray	2.35	A	2-357	[»]
	1QXL	X-ray	2.25	A	2-357	[»]
	1UML	X-ray	2.50	A	2-357	[»]
	1V79	X-ray	2.50	A	2-357	[»]
	1V7A	X-ray	2.50	A	2-357	[»]
	1VFL	X-ray	1.80	A	2-357	[»]
	1W1I	X-ray	3.03	E/F/G/H	1-357	[»]
	1WXY	X-ray	2.50	A	2-357	[»]
	1WXZ	X-ray	2.80	A	2-357	[»]
	2BGN	X-ray	3.15	E/F/G/H	2-363	[»]
	2E1W	X-ray	2.50	A	2-357	[»]
	2Z7G	X-ray	2.52	A	2-357	[»]
ProteinModelPortalⁱ	P56658
SMRⁱ	P56658
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

IntActⁱ	P56658, 1 interactor
MINTⁱ	P56658
STRINGⁱ	9913.ENSBTAP00000006947

Chemistry databases

BindingDBⁱ	P56658
ChEMBLⁱ	CHEMBL2966

Proteomic databases

PaxDbⁱ	P56658
PeptideAtlasⁱ	P56658
PRIDEⁱ	P56658

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

GeneIDⁱ	280712
KEGGⁱ	bta:280712

Organism-specific databases

CTDⁱ	100

CTDⁱ

100

Phylogenomic databases

eggNOGⁱ	KOG1097 Eukaryota COG1816 LUCA
HOGENOMⁱ	HOG000218816
HOVERGENⁱ	HBG001718
InParanoidⁱ	P56658
KOⁱ	K01488

Enzyme and pathway databases

BRENDAⁱ	3.5.4.4 908

BRENDAⁱ

3.5.4.4 908

Miscellaneous databases

EvolutionaryTraceⁱ	P56658
Protein Ontology More...PROⁱ	PR:P56658

Family and domain databases

CDDⁱ	cd01320 ADA, 1 hit
HAMAPⁱ	MF_00540 A_deaminase, 1 hit
InterProⁱ	View protein in InterPro IPR006650 A/AMP_deam_AS IPR001365 A/AMP_deaminase_dom IPR028893 A_deaminase IPR006330 Ado/ade_deaminase IPR032466 Metal_Hydrolase
Pfamⁱ	View protein in Pfam PF00962 A_deaminase, 1 hit
SUPFAMⁱ	SSF51556 SSF51556, 1 hit
TIGRFAMsⁱ	TIGR01430 aden_deam, 1 hit
PROSITEⁱ	View protein in PROSITE PS00485 A_DEAMINASE, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	ADA_BOVIN
Accessionⁱ	P56658Primary (citable) accession number: P56658 Secondary accession number(s): Q9MYY1
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
	Last sequence update:	January 23, 2007
	Last modified:	February 28, 2018
	This is version 131 of the entry and version 3 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Direct protein sequencing, Pharmaceutical, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
PDB cross-references
Index of Protein Data Bank (PDB) cross-references

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UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P56658 (ADA_BOVIN)

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Adenosine deaminase

ADA

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Plasma membrane

Lysosome

Other locations

Cytosol

Lysosome

Plasma Membrane

Other locations

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Chemistry databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

Chemistry databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Experimental Info

<p>This subsection of the ‘Sequence’ section reports information derived from mass spectrometry experiments done on the entire protein or on biologically active derived peptide(s).<p><a href='/help/mass_spectrometry' target='_top'>More...</a></p>Mass spectrometryi

Natural variant

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

<p>This subsection of the <a href="http://www.uniprot.org/manual/cross_references_section">Cross-references</a> section provides links to various web resources that are relevant for a specific protein.<p><a href='/help/web_resource' target='_top'>More...</a></p>Web resourcesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Chemistry databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Mass spectrometryⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Web resourcesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ