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UniProtKB - P56655 (CP238_MOUSE)
Protein
Cytochrome P450 2C38
Gene
Cyp2c38
Organism
Mus musculus (Mouse)
Status
Functioni
A cytochrome P450 monooxygenase that primarily catalyzes the epoxidation of 11,12 double bond of (5Z,8Z,11Z,14Z)-eicosatetraenoic acid (arachidonate) forming 11,12-epoxyeicosatrienoic acid (11,12-EET) regioisomer. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH--hemoprotein reductase).
1 PublicationCatalytic activityi
- an organic molecule + O2 + reduced [NADPH—hemoprotein reductase] = an alcohol + H+ + H2O + oxidized [NADPH—hemoprotein reductase]1 PublicationEC:1.14.14.11 PublicationThis reaction proceeds in the forward1 Publication direction.
- (5Z,8Z,11Z,14Z)-eicosatetraenoate + O2 + reduced [NADPH—hemoprotein reductase] = 11,12-epoxy-(5Z,8Z,14Z)-eicosatrienoate + H+ + H2O + oxidized [NADPH—hemoprotein reductase]1 PublicationThis reaction proceeds in the forward1 Publication direction.
Cofactori
hemeBy similarity
: arachidonate metabolism Pathwayi
This protein is involved in the pathway arachidonate metabolism, which is part of Lipid metabolism.1 PublicationView all proteins of this organism that are known to be involved in the pathway arachidonate metabolism and in Lipid metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 435 | Iron (heme axial ligand)By similarity | 1 |
GO - Molecular functioni
- arachidonic acid 11,12-epoxygenase activity Source: UniProtKB
- arachidonic acid 14,15-epoxygenase activity Source: UniProtKB
- arachidonic acid epoxygenase activity Source: MGI
- aromatase activity Source: UniProtKB-EC
- caffeine oxidase activity Source: MGI
- enzyme binding Source: MGI
- heme binding Source: MGI
- iron ion binding Source: InterPro
- monooxygenase activity Source: MGI
- oxidoreductase activity Source: MGI
- oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen Source: GO_Central
- steroid hydroxylase activity Source: MGI
GO - Biological processi
- arachidonic acid metabolic process Source: UniProtKB
- epoxygenase P450 pathway Source: GO_Central
- organic acid metabolic process Source: GO_Central
- xenobiotic metabolic process Source: GO_Central
Keywordsi
Molecular function | Monooxygenase, Oxidoreductase |
Biological process | Lipid metabolism |
Ligand | Heme, Iron, Metal-binding |
Enzyme and pathway databases
UniPathwayi | UPA00383 |
Chemistry databases
SwissLipidsi | SLP:000001669 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1306819, Cyp2c38 |
VEuPathDBi | HostDB:ENSMUSG00000032808 |
Subcellular locationi
Endoplasmic reticulum
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Other locations
- cytoplasm Source: GO_Central
- intracellular membrane-bounded organelle Source: MGI
Keywords - Cellular componenti
Endoplasmic reticulum, Membrane, MicrosomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 20 | Sequence analysisAdd BLAST | 20 | |
ChainiPRO_0000051721 | 21 – 490 | Cytochrome P450 2C38Sequence analysisAdd BLAST | 470 |
Proteomic databases
jPOSTi | P56655 |
MaxQBi | P56655 |
PaxDbi | P56655 |
PeptideAtlasi | P56655 |
PRIDEi | P56655 |
PTM databases
iPTMneti | P56655 |
PhosphoSitePlusi | P56655 |
Expressioni
Tissue specificityi
Liver, brain, kidney, and intestine, with trace amounts in lung and heart.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000032808, Expressed in liver and 39 other tissues |
Genevisiblei | P56655, MM |
Interactioni
GO - Molecular functioni
- enzyme binding Source: MGI
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000044722 |
Miscellaneous databases
RNActi | P56655, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the cytochrome P450 family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG0156, Eukaryota |
GeneTreei | ENSGT00940000155736 |
HOGENOMi | CLU_001570_22_0_1 |
InParanoidi | P56655 |
OMAi | AVLCYFW |
OrthoDBi | 702827at2759 |
PhylomeDBi | P56655 |
TreeFami | TF352043 |
Family and domain databases
Gene3Di | 1.10.630.10, 1 hit |
InterProi | View protein in InterPro IPR001128, Cyt_P450 IPR017972, Cyt_P450_CS IPR002401, Cyt_P450_E_grp-I IPR036396, Cyt_P450_sf |
Pfami | View protein in Pfam PF00067, p450, 1 hit |
PRINTSi | PR00463, EP450I PR00385, P450 |
SUPFAMi | SSF48264, SSF48264, 1 hit |
PROSITEi | View protein in PROSITE PS00086, CYTOCHROME_P450, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P56655-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDLVTFLVLT LSSLILLSLW RQRSRRGRLP PGPTPFPIIG NFLQIDVKNF
60 70 80 90 100
NQSLTNFSKT YGPVFTLYLG SRPIVVLHGY EAVKEALIDH GEEFSGRENI
110 120 130 140 150
PMSEKINNGL GITFSNGNSW KETRRFTLMT LRNLGMGKRN IEDRVREEAQ
160 170 180 190 200
CLVEELRKTK GSPCDPTFIL SCAPCNVICS IIFQDRFDYK DKDFLMLMKK
210 220 230 240 250
LNENVKILSS PWLQVCNNFP LLIDYCPGSH HKVLKNFKYI RSYLLEKVKE
260 270 280 290 300
HQESLDVTNP RDFIDYFLIK QKQANHIEQA EYSLENLVCT INNLFAAGTE
310 320 330 340 350
TTSTTLRYAL LLLMKYPDVT AKVQEEIDHV VGRHRSPCMQ DRSRMPYTDA
360 370 380 390 400
MIHEVQRFIN LVPNNLPHAV TCDIKFRNYI IPKGTTVVTS LTSVLHDSKE
410 420 430 440 450
FPNPEMFDPG HFLDANGNFK KSDYFMTFSA GKRVCAGEGL ARMELFLILT
460 470 480 490
TILQNFKLKS LVHPKDIDMI PFVNGLITLP PHYQLCFIPL
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 60 | T → A in AAD13720 (PubMed:9721182).Curated | 1 | |
Sequence conflicti | 60 | T → A in AAI50722 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 98 | E → G in AAD13720 (PubMed:9721182).Curated | 1 | |
Sequence conflicti | 98 | E → G in AAI50722 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 125 | R → H in AAD13720 (PubMed:9721182).Curated | 1 | |
Sequence conflicti | 125 | R → H in AAI50722 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 188 | D → H in AAD13720 (PubMed:9721182).Curated | 1 | |
Sequence conflicti | 188 | D → H in AAI50722 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 253 | E → A in AAD13720 (PubMed:9721182).Curated | 1 | |
Sequence conflicti | 253 | E → A in AAI50722 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 257 | V → F in AAD13720 (PubMed:9721182).Curated | 1 | |
Sequence conflicti | 257 | V → F in AAI50722 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 344 | R → H in AAD13720 (PubMed:9721182).Curated | 1 | |
Sequence conflicti | 344 | R → H in AAI50722 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 470 | I → M in AAD13720 (PubMed:9721182).Curated | 1 | |
Sequence conflicti | 470 | I → M in AAI50722 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 478 | T → A in AAD13720 (PubMed:9721182).Curated | 1 | |
Sequence conflicti | 478 | T → A in AAI50722 (PubMed:15489334).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF047725 mRNA Translation: AAD13720.1 AC139233 Genomic DNA No translation available. BC150721 mRNA Translation: AAI50722.1 |
CCDSi | CCDS29794.1 |
RefSeqi | NP_034132.2, NM_010002.3 |
Genome annotation databases
Ensembli | ENSMUST00000035488; ENSMUSP00000044722; ENSMUSG00000032808 |
GeneIDi | 13097 |
KEGGi | mmu:13097 |
UCSCi | uc008hka.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF047725 mRNA Translation: AAD13720.1 AC139233 Genomic DNA No translation available. BC150721 mRNA Translation: AAI50722.1 |
CCDSi | CCDS29794.1 |
RefSeqi | NP_034132.2, NM_010002.3 |
3D structure databases
SMRi | P56655 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000044722 |
Chemistry databases
SwissLipidsi | SLP:000001669 |
PTM databases
iPTMneti | P56655 |
PhosphoSitePlusi | P56655 |
Proteomic databases
jPOSTi | P56655 |
MaxQBi | P56655 |
PaxDbi | P56655 |
PeptideAtlasi | P56655 |
PRIDEi | P56655 |
Protocols and materials databases
DNASUi | 13097 |
Genome annotation databases
Ensembli | ENSMUST00000035488; ENSMUSP00000044722; ENSMUSG00000032808 |
GeneIDi | 13097 |
KEGGi | mmu:13097 |
UCSCi | uc008hka.1, mouse |
Organism-specific databases
CTDi | 13097 |
MGIi | MGI:1306819, Cyp2c38 |
VEuPathDBi | HostDB:ENSMUSG00000032808 |
Phylogenomic databases
eggNOGi | KOG0156, Eukaryota |
GeneTreei | ENSGT00940000155736 |
HOGENOMi | CLU_001570_22_0_1 |
InParanoidi | P56655 |
OMAi | AVLCYFW |
OrthoDBi | 702827at2759 |
PhylomeDBi | P56655 |
TreeFami | TF352043 |
Enzyme and pathway databases
UniPathwayi | UPA00383 |
Miscellaneous databases
BioGRID-ORCSi | 13097, 0 hits in 66 CRISPR screens |
ChiTaRSi | Cyp2c38, mouse |
PROi | PR:P56655 |
RNActi | P56655, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000032808, Expressed in liver and 39 other tissues |
Genevisiblei | P56655, MM |
Family and domain databases
Gene3Di | 1.10.630.10, 1 hit |
InterProi | View protein in InterPro IPR001128, Cyt_P450 IPR017972, Cyt_P450_CS IPR002401, Cyt_P450_E_grp-I IPR036396, Cyt_P450_sf |
Pfami | View protein in Pfam PF00067, p450, 1 hit |
PRINTSi | PR00463, EP450I PR00385, P450 |
SUPFAMi | SSF48264, SSF48264, 1 hit |
PROSITEi | View protein in PROSITE PS00086, CYTOCHROME_P450, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CP238_MOUSE | |
Accessioni | P56655Primary (citable) accession number: P56655 Secondary accession number(s): B2RWV4, F8VPK7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1999 |
Last sequence update: | October 3, 2012 | |
Last modified: | February 23, 2022 | |
This is version 152 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families