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Protein

Alpha-conotoxin MII

Gene
N/A
Organism
Conus magus (Magus cone) (Magician's cone snail)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Alpha-conotoxins bind to the nicotinic acetylcholine receptors (nAChR) and inhibit them. This toxin blocks neuronal mammalian nAChRs (alpha-6/alpha-3-beta-2-beta-3 (0.39 nM) > alpha-3-beta-2 > alpha-3-beta-4 = alpha-4-beta-2).5 Publications

Miscellaneous

There are currently a number of patents describing the use of this peptide in therapeutic applications.

GO - Molecular functioni

Keywordsi

Molecular functionAcetylcholine receptor inhibiting toxin, Ion channel impairing toxin, Neurotoxin, Postsynaptic neurotoxin, Toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-conotoxin MII1 Publication
Short name:
Alpha-Ctx MII1 Publication
Short name:
Alpha-MII1 Publication
OrganismiConus magus (Magus cone) (Magician's cone snail)
Taxonomic identifieri6492 [NCBI]
Taxonomic lineageiEukaryotaMetazoaLophotrochozoaMolluscaGastropodaCaenogastropodaNeogastropodaConoideaConidaeConusPionoconus

Organism-specific databases

ConoServeri8 MII precursor

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi49G → A: 4.5-fold decrease in inhibition of alpha-3/beta-2 nAChR. 1 Publication1
Mutagenesisi52S → A: 4.9-fold decrease in inhibition of alpha-3/beta-2 nAChR. 1 Publication1
Mutagenesisi53N → A: >2700-fold decrease in inhibition of alpha-3/beta-2 nAChR. 1 Publication1
Mutagenesisi54P → A: 700-fold decrease in inhibition of alpha-3/beta-2 nAChR. 1 Publication1
Mutagenesisi55V → A: 2.5-fold decrease in inhibition of alpha-3/beta-2 nAChR. 1 Publication1
Mutagenesisi57H → A: 2-fold and ~20-fold decrease in ability to inhibit alpha-6/alpha-3-beta-2-beta-3 and alpha-3-beta-2, respectively. In MII[H9A;L15A]; exhibits preferential loss of activity at alpha-3-beta-2 versus alpha-6/alpha-3-beta-2-beta-3 nAChR, making this analog the most selective alpha-6 ligand known. 2 Publications1
Mutagenesisi58L → A: 1.5-fold decrease in inhibition of alpha-3/beta-2 nAChR. 1 Publication1
Mutagenesisi59E → A: 4.6-fold decrease in inhibition of alpha-3/beta-2 nAChR. 1 Publication1
Mutagenesisi60H → A: About 2700-fold decrease in inhibition of alpha-3/beta-2 nAChR. 1 Publication1
Mutagenesisi61S → A: 2.3-fold decrease in inhibition of alpha-3/beta-2 nAChR. 1 Publication1
Mutagenesisi62N → A: No change in inhibition of alpha-3/beta-2 nAChR. 1 Publication1
Mutagenesisi63L → A: 2.4-fold and 15-fold decrease in ability to inhibit alpha-6/alpha-3-beta-2-beta-3 and alpha-3-beta-2, respectively. In MII[H9A;L15A]; exhibits preferential loss of activity at alpha-3-beta-2 versus alpha-6/alpha-3-beta-2-beta-3 nAChR, making this analog the most selective alpha-6 ligand thus far reported. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
PropeptideiPRO_000003488122 – 481 PublicationAdd BLAST27
PeptideiPRO_000003488249 – 64Alpha-conotoxin MII1 PublicationAdd BLAST16

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi50 ↔ 563 PublicationsImported
Disulfide bondi51 ↔ 643 PublicationsImported
Modified residuei64Cysteine amide2 Publications1

Keywords - PTMi

Amidation, Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom duct.1 Publication

Structurei

Secondary structure

168
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP56636
SMRiP56636
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP56636

Family & Domainsi

Domaini

The cysteine framework is I (CC-C-C). Alpha4/7 pattern.Curated

Sequence similaritiesi

Belongs to the conotoxin A superfamily.Curated

Keywords - Domaini

Signal

Family and domain databases

InterProiView protein in InterPro
IPR009958 Conotoxin_a-typ
IPR018072 Conotoxin_a-typ_CS
PfamiView protein in Pfam
PF07365 Toxin_8, 1 hit
PROSITEiView protein in PROSITE
PS60014 ALPHA_CONOTOXIN, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P56636-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGMRMMFTVF LLVVLATTVV SFPSDRASDG RNAAANDKAS DVITLALKGC
60
CSNPVCHLEH SNLCGRRR
Length:68
Mass (Da):7,357
Last modified:September 5, 2006 - v3
Checksum:iFBD9AB40E6F277DF
GO

Mass spectrometryi

Molecular mass is 1710.6 Da from positions 49 - 64. Determined by LSI. 1 Publication

Sequence databases

PIRiA59046

Similar proteinsi

Cross-referencesi

Sequence databases

PIRiA59046

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1M2CNMR-A49-64[»]
1MIINMR-A49-64[»]
2AJWNMR-A49-64[»]
2AK0NMR-A49-64[»]
ProteinModelPortaliP56636
SMRiP56636
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

ConoServeri8 MII precursor

Miscellaneous databases

EvolutionaryTraceiP56636

Family and domain databases

InterProiView protein in InterPro
IPR009958 Conotoxin_a-typ
IPR018072 Conotoxin_a-typ_CS
PfamiView protein in Pfam
PF07365 Toxin_8, 1 hit
PROSITEiView protein in PROSITE
PS60014 ALPHA_CONOTOXIN, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCA12_CONMA
AccessioniPrimary (citable) accession number: P56636
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: September 5, 2006
Last modified: September 12, 2018
This is version 94 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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