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UniProtKB - P56580 (PTHB_ECOLI)
Protein
PTS system glucitol/sorbitol-specific EIIB component
Gene
srlE
Organism
Escherichia coli (strain K12)
Status
Functioni
The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. The enzyme II complex composed of SrlA, SrlB and SrlE is involved in glucitol/sorbitol transport. It can also use D-mannitol.
1 PublicationCatalytic activityi
- D-sorbitol(out) + Nπ-phospho-L-histidyl-[protein] = D-sorbitol 6-phosphate(in) + L-histidyl-[protein]1 PublicationEC:2.7.1.1981 Publication
Kineticsi
- KM=44 µM for D-glucitol1 Publication
- KM=60 µM for D-mannitol1 Publication
- Vmax=7.2 nmol/min/mg enzyme with D-mannitol as substrate1 Publication
- Vmax=0.83 nmol/min/mg enzyme with D-glucitol as substrate1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 71 | Phosphocysteine intermediate; for EIIB activityCurated | 1 |
GO - Molecular functioni
- kinase activity Source: UniProtKB-KW
- protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Source: InterPro
- protein-phosphocysteine-sugar phosphotransferase activity Source: UniProtKB
GO - Biological processi
- phosphoenolpyruvate-dependent sugar phosphotransferase system Source: UniProtKB
Keywordsi
Molecular function | Kinase, Transferase |
Biological process | Phosphotransferase system, Sugar transport, Transport |
Enzyme and pathway databases
BioCyci | EcoCyc:GUTA-MONOMER |
Protein family/group databases
TCDBi | 4.A.4.1.1, the pts glucitol (gut) family |
Names & Taxonomyi
Protein namesi | Recommended name: PTS system glucitol/sorbitol-specific EIIB component1 Publication (EC:2.7.1.1981 Publication)Alternative name(s): EII-Gut1 Publication Enzyme II-Gut1 Publication Glucitol/sorbitol-specific phosphotransferase enzyme IIB component1 Publication |
Gene namesi | Name:srlE Synonyms:gutA1 Publication, gutE Ordered Locus Names:b2703, JW5430 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell inner membrane 1 Publication; Multi-pass membrane protein Sequence analysis1 Publication
Plasma Membrane
- plasma membrane Source: EcoCyc
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 176 | CytoplasmicSequence analysisAdd BLAST | 176 | |
Transmembranei | 177 – 197 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 198 – 199 | PeriplasmicSequence analysis | 2 | |
Transmembranei | 200 – 220 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 221 – 228 | CytoplasmicSequence analysis | 8 | |
Transmembranei | 229 – 249 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 250 – 256 | PeriplasmicSequence analysis | 7 | |
Transmembranei | 257 – 277 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 278 – 296 | CytoplasmicSequence analysisAdd BLAST | 19 | |
Transmembranei | 297 – 317 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 318 – 319 | PeriplasmicSequence analysis | 2 |
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000186562 | 1 – 319 | PTS system glucitol/sorbitol-specific EIIB componentAdd BLAST | 319 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 71 | Phosphocysteine; by EIIAPROSITE-ProRule annotation | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | P56580 |
PaxDbi | P56580 |
PRIDEi | P56580 |
Expressioni
Inductioni
Regulated by an unusual system which consists of the activator GutM and the repressor GutR in addition to the cAMP-CRP complex.1 Publication
Interactioni
Protein-protein interaction databases
BioGRIDi | 4262075, 19 interactors |
ComplexPortali | CPX-5969, Glucitol/sorbitol enzyme II complex |
STRINGi | 511145.b2703 |
Structurei
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 178 | PTS EIIB type-5PROSITE-ProRule annotationAdd BLAST | 178 |
Domaini
The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.PROSITE-ProRule annotation
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG3732, Bacteria |
HOGENOMi | CLU_054195_0_0_6 |
InParanoidi | P56580 |
PhylomeDBi | P56580 |
Family and domain databases
InterProi | View protein in InterPro IPR011638, PTS_EIIBC_GUT_C IPR011618, PTS_EIIBC_GUT_N IPR004702, PTS_sorb_EIIBC |
PANTHERi | PTHR39427, PTHR39427, 1 hit |
Pfami | View protein in Pfam PF07663, EIIBC-GUT_C, 1 hit PF03612, EIIBC-GUT_N, 1 hit |
TIGRFAMsi | TIGR00825, EIIBC-GUT, 1 hit |
PROSITEi | View protein in PROSITE PS51102, PTS_EIIB_TYPE_5, 1 hit |
i Sequence
Sequence statusi: Complete.
P56580-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTHIRIEKGT GGWGGPLELK ATPGKKIVYI TAGTRPAIVD KLAQLTGWQA
60 70 80 90 100
IDGFKEGEPA EAEIGVAVID CGGTLRCGIY PKRRIPTINI HSTGKSGPLA
110 120 130 140 150
QYIVEDIYVS GVKEENITVV GDATPQPSSV GRDYDTSKKI TEQSDGLLAK
160 170 180 190 200
VGMGMGSTVA VLFQSGRDTI DTVLKTILPF MAFVSALIGI IMASGLGDWI
210 220 230 240 250
AHGLAPLASH PLGLVMLALI CSFPLLSPFL GPGAVIAQVI GVLIGVQIGL
260 270 280 290 300
GNIPPHLALP ALFAINAQAA CDFIPVGLSL AEARQDTVRV GVPSVLVSRF
310
LTGAPTVLIA WFVSGFIYQ
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J02708 Genomic DNA Translation: AAC13416.1 U00096 Genomic DNA Translation: AAT48149.1 AP009048 Genomic DNA Translation: BAA16564.2 |
PIRi | A26725, WQEC2S |
RefSeqi | WP_000148878.1, NZ_LN832404.1 YP_026181.1, NC_000913.3 |
Genome annotation databases
EnsemblBacteriai | AAT48149; AAT48149; b2703 BAA16564; BAA16564; BAA16564 |
GeneIDi | 948933 |
KEGGi | ecj:JW5430 eco:b2703 |
PATRICi | fig|1411691.4.peg.4039 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J02708 Genomic DNA Translation: AAC13416.1 U00096 Genomic DNA Translation: AAT48149.1 AP009048 Genomic DNA Translation: BAA16564.2 |
PIRi | A26725, WQEC2S |
RefSeqi | WP_000148878.1, NZ_LN832404.1 YP_026181.1, NC_000913.3 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 4262075, 19 interactors |
ComplexPortali | CPX-5969, Glucitol/sorbitol enzyme II complex |
STRINGi | 511145.b2703 |
Protein family/group databases
TCDBi | 4.A.4.1.1, the pts glucitol (gut) family |
Proteomic databases
jPOSTi | P56580 |
PaxDbi | P56580 |
PRIDEi | P56580 |
Genome annotation databases
EnsemblBacteriai | AAT48149; AAT48149; b2703 BAA16564; BAA16564; BAA16564 |
GeneIDi | 948933 |
KEGGi | ecj:JW5430 eco:b2703 |
PATRICi | fig|1411691.4.peg.4039 |
Organism-specific databases
EchoBASEi | EB4116 |
Phylogenomic databases
eggNOGi | COG3732, Bacteria |
HOGENOMi | CLU_054195_0_0_6 |
InParanoidi | P56580 |
PhylomeDBi | P56580 |
Enzyme and pathway databases
BioCyci | EcoCyc:GUTA-MONOMER |
Miscellaneous databases
PROi | PR:P56580 |
Family and domain databases
InterProi | View protein in InterPro IPR011638, PTS_EIIBC_GUT_C IPR011618, PTS_EIIBC_GUT_N IPR004702, PTS_sorb_EIIBC |
PANTHERi | PTHR39427, PTHR39427, 1 hit |
Pfami | View protein in Pfam PF07663, EIIBC-GUT_C, 1 hit PF03612, EIIBC-GUT_N, 1 hit |
TIGRFAMsi | TIGR00825, EIIBC-GUT, 1 hit |
PROSITEi | View protein in PROSITE PS51102, PTS_EIIB_TYPE_5, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PTHB_ECOLI | |
Accessioni | P56580Primary (citable) accession number: P56580 Secondary accession number(s): P05705, P78103, P78215 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 15, 1998 |
Last sequence update: | December 15, 1998 | |
Last modified: | February 23, 2022 | |
This is version 157 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |