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Protein

Lens fiber membrane intrinsic protein

Gene

Lim2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Present in the thicker 16-17 nm junctions of mammalian lens fiber cells, where it may contribute to cell junctional organization. Acts as a receptor for calmodulin. May play an important role in both lens development and cataractogenesis.

GO - Molecular functioni

  • structural constituent of eye lens Source: MGI

GO - Biological processi

  • camera-type eye development Source: MGI
  • lens development in camera-type eye Source: MGI

Keywordsi

Molecular functionEye lens protein

Names & Taxonomyi

Protein namesi
Recommended name:
Lens fiber membrane intrinsic protein
Alternative name(s):
MP17
MP18
MP19
MP20
Gene namesi
Name:Lim2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:104698 Lim2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 3CytoplasmicSequence analysis3
Transmembranei4 – 24HelicalSequence analysisAdd BLAST21
Topological domaini25 – 66ExtracellularSequence analysisAdd BLAST42
Transmembranei67 – 87HelicalSequence analysisAdd BLAST21
Topological domaini88 – 98CytoplasmicSequence analysisAdd BLAST11
Transmembranei99 – 119HelicalSequence analysisAdd BLAST21
Topological domaini120 – 140ExtracellularSequence analysisAdd BLAST21
Transmembranei141 – 161HelicalSequence analysisAdd BLAST21
Topological domaini162 – 173CytoplasmicSequence analysisAdd BLAST12

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Involvement in diseasei

Defects in Lim2 are the cause of the cataractous mouse mutant with total opacity of lens 3 (To3). Mice heterozygous or homozygous for the To3 mutation have total opacity of the lens with a dense cataract. In addition, the To3/To3 homozygotes exhibit microphthalmia, abnormally small eyes.1 Publication

Keywords - Diseasei

Disease mutation

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001646651 – 173Lens fiber membrane intrinsic proteinAdd BLAST173

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi43C-linked (Man) tryptophanBy similarity1
Glycosylationi61C-linked (Man) tryptophanBy similarity1
Glycosylationi62N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei171PhosphothreonineBy similarity1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP56563
PRIDEiP56563

PTM databases

PhosphoSitePlusiP56563

Expressioni

Gene expression databases

BgeeiENSMUSG00000004613 Expressed in 4 organ(s), highest expression level in interparietal bone primordium
CleanExiMM_LIM2
GenevisibleiP56563 MM

Interactioni

Subunit structurei

Seems to be associated with itself or another lens membrane component via disulfide bonds.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000004732

Structurei

3D structure databases

ProteinModelPortaliP56563
SMRiP56563
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PMP-22/EMP/MP20 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IN7P Eukaryota
ENOG4111H51 LUCA
GeneTreeiENSGT00510000046994
HOGENOMiHOG000116021
HOVERGENiHBG003671
InParanoidiP56563
OMAiRYCLGSK
OrthoDBiEOG091G0MCH
PhylomeDBiP56563
TreeFamiTF330587

Family and domain databases

InterProiView protein in InterPro
IPR003935 LMIP
IPR004031 PMP22/EMP/MP20/Claudin
IPR004032 PMP22_EMP_MP20
PfamiView protein in Pfam
PF00822 PMP22_Claudin, 1 hit
PRINTSiPR01453 EPMEMFAMILY
PR01457 LENSMEMPROT
PROSITEiView protein in PROSITE
PS01221 PMP22_1, 1 hit
PS01222 PMP22_2, 1 hit

Sequencei

Sequence statusi: Complete.

P56563-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MYSFMGGGLF CAWVGTILLV VATATDHWMQ YRLSGSFAHQ GLWRYCLGNK
60 70 80 90 100
CFLQTESIAY WNATRAFMIL SALCATSGII MGVLAFAQQS TFTRLSRPFS
110 120 130 140 150
AGIMFFASTL FVLLALAIYT GVTVSFLGRR FGDWRFSWSY ILGWVALLMT
160 170
FFAGIFYMCA YRMHECRRLA TPR
Length:173
Mass (Da):19,621
Last modified:May 10, 2002 - v2
Checksum:iC41FA9CF0618D57F
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti15G → V in To3. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF320075 Genomic DNA Translation: AAK08062.1
AK029354 mRNA Translation: BAC26414.1
AK164958 mRNA Translation: BAE37979.1
BC119253 mRNA Translation: AAI19254.1
BC119255 mRNA Translation: AAI19256.1
CCDSiCCDS21168.1
RefSeqiNP_808361.1, NM_177693.3
UniGeneiMm.267027

Genome annotation databases

EnsembliENSMUST00000004732; ENSMUSP00000004732; ENSMUSG00000093639
GeneIDi233187
KEGGimmu:233187
UCSCiuc009gmp.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF320075 Genomic DNA Translation: AAK08062.1
AK029354 mRNA Translation: BAC26414.1
AK164958 mRNA Translation: BAE37979.1
BC119253 mRNA Translation: AAI19254.1
BC119255 mRNA Translation: AAI19256.1
CCDSiCCDS21168.1
RefSeqiNP_808361.1, NM_177693.3
UniGeneiMm.267027

3D structure databases

ProteinModelPortaliP56563
SMRiP56563
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000004732

PTM databases

PhosphoSitePlusiP56563

Proteomic databases

PaxDbiP56563
PRIDEiP56563

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000004732; ENSMUSP00000004732; ENSMUSG00000093639
GeneIDi233187
KEGGimmu:233187
UCSCiuc009gmp.1 mouse

Organism-specific databases

CTDi3982
MGIiMGI:104698 Lim2

Phylogenomic databases

eggNOGiENOG410IN7P Eukaryota
ENOG4111H51 LUCA
GeneTreeiENSGT00510000046994
HOGENOMiHOG000116021
HOVERGENiHBG003671
InParanoidiP56563
OMAiRYCLGSK
OrthoDBiEOG091G0MCH
PhylomeDBiP56563
TreeFamiTF330587

Miscellaneous databases

PROiPR:P56563
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000004613 Expressed in 4 organ(s), highest expression level in interparietal bone primordium
CleanExiMM_LIM2
GenevisibleiP56563 MM

Family and domain databases

InterProiView protein in InterPro
IPR003935 LMIP
IPR004031 PMP22/EMP/MP20/Claudin
IPR004032 PMP22_EMP_MP20
PfamiView protein in Pfam
PF00822 PMP22_Claudin, 1 hit
PRINTSiPR01453 EPMEMFAMILY
PR01457 LENSMEMPROT
PROSITEiView protein in PROSITE
PS01221 PMP22_1, 1 hit
PS01222 PMP22_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiLMIP_MOUSE
AccessioniPrimary (citable) accession number: P56563
Secondary accession number(s): Q0VEF3, Q3TNV8, Q99PA6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: May 10, 2002
Last modified: November 7, 2018
This is version 131 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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