UniProtKB - P56459 (SYDND_HELPY)
Protein
Aspartate--tRNA(Asp/Asn) ligase
Gene
aspS
Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Functioni
Aspartyl-tRNA synthetase with relaxed tRNA specificity since it is able to aspartylate not only its cognate tRNA(Asp) but also tRNA(Asn). Is 1.7 times more efficient at aminoacylating tRNA(Asp) over tRNA(Asn). Reaction proceeds in two steps: L-aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp/Asn).1 Publication
Catalytic activityi
- EC:6.1.1.23UniRule annotation1 Publication
Kineticsi
kcat is 0.022 sec(-1) for tRNA(Asp) aspartylation and 0.014 sec(-1) for tRNA(Asn) aspartylation.
- KM=0.77 µM for tRNA(Asp)1 Publication
- KM=0.83 µM for tRNA(Asn)1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 30 | Important for tRNA non-discriminationUniRule annotation | 1 | |
Sitei | 80 | Important for tRNA non-discriminationUniRule annotation | 1 | |
Binding sitei | 171 | AspartateUniRule annotation | 1 | |
Binding sitei | 217 | AspartateUniRule annotation | 1 | |
Binding sitei | 226 | ATPUniRule annotation | 1 | |
Binding sitei | 444 | AspartateUniRule annotation | 1 | |
Binding sitei | 474 | ATPUniRule annotation | 1 | |
Binding sitei | 481 | AspartateUniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 217 – 219 | ATPUniRule annotation | 3 | |
Nucleotide bindingi | 526 – 529 | ATPUniRule annotation | 4 |
GO - Molecular functioni
- aspartate-tRNA(Asn) ligase activity Source: UniProtKB-EC
- aspartate-tRNA ligase activity Source: UniProtKB-UniRule
- ATP binding Source: UniProtKB-UniRule
- nucleic acid binding Source: InterPro
GO - Biological processi
- aspartyl-tRNA aminoacylation Source: UniProtKB-UniRule
Keywordsi
Molecular function | Aminoacyl-tRNA synthetase, Ligase |
Biological process | Protein biosynthesis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | HPY:HP0617-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Aspartate--tRNA(Asp/Asn) ligaseUniRule annotation (EC:6.1.1.23UniRule annotation)Alternative name(s): Aspartyl-tRNA synthetaseUniRule annotation Short name: AspRSUniRule annotation Non-discriminating aspartyl-tRNA synthetaseUniRule annotation Short name: ND-AspRSUniRule annotation |
Gene namesi | Name:aspSUniRule annotation Ordered Locus Names:HP_0617 |
Organismi | Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) |
Taxonomic identifieri | 85962 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter › |
Proteomesi |
|
Subcellular locationi
- Cytoplasm UniRule annotation
GO - Cellular componenti
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 81 | L → N: Enhances enzyme specificity for tRNA(Asp) over tRNA(Asn), by reducing enzyme's ability to misacylate tRNA(Asn). 1 Publication | 1 | |
Mutagenesisi | 86 | L → M: Enhances enzyme specificity for tRNA(Asp) over tRNA(Asn), by reducing enzyme's ability to misacylate tRNA(Asn). 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000110883 | 1 – 577 | Aspartate--tRNA(Asp/Asn) ligaseAdd BLAST | 577 |
Proteomic databases
PaxDbi | P56459 |
Interactioni
Subunit structurei
Homodimer.
UniRule annotationProtein-protein interaction databases
DIPi | DIP-3174N |
IntActi | P56459, 2 interactors |
MINTi | P56459 |
STRINGi | 85962.C694_03190 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P56459 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 195 – 198 | AspartateUniRule annotation | 4 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0173, Bacteria |
OMAi | YQLDVEM |
Family and domain databases
Gene3Di | 3.30.1360.30, 1 hit |
HAMAPi | MF_00044, Asp_tRNA_synth_type1, 1 hit |
InterProi | View protein in InterPro IPR004364, Aa-tRNA-synt_II IPR006195, aa-tRNA-synth_II IPR004524, Asp-tRNA-ligase_1 IPR002312, Asp/Asn-tRNA-synth_IIb IPR004115, GAD-like_sf IPR029351, GAD_dom IPR012340, NA-bd_OB-fold IPR004365, NA-bd_OB_tRNA |
PANTHERi | PTHR22594:SF5, PTHR22594:SF5, 1 hit |
Pfami | View protein in Pfam PF02938, GAD, 1 hit PF00152, tRNA-synt_2, 1 hit PF01336, tRNA_anti-codon, 1 hit |
PRINTSi | PR01042, TRNASYNTHASP |
SUPFAMi | SSF50249, SSF50249, 1 hit SSF55261, SSF55261, 1 hit |
TIGRFAMsi | TIGR00459, aspS_bact, 1 hit |
PROSITEi | View protein in PROSITE PS50862, AA_TRNA_LIGASE_II, 1 hit |
i Sequence
Sequence statusi: Complete.
P56459-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRSHFCTEIS EKDVGKIVKV AGWCNTYRDH GGVVFIDLRD KSGLVQLVCD
60 70 80 90 100
PSSKAYEKAL EVRSEFVLVA KGKVRLRGAG LENPKLKTGK IEIVLEELII
110 120 130 140 150
ENKSATPPIE IGNKHVNEDL RLKYRYLDLR SPNSYEIFKL RSEVALITRN
160 170 180 190 200
TLAQKGFLEI ETPILSKTTP EGARDYLVPS RVHEGEFFAL PQSPQLFKQL
210 220 230 240 250
LMVGGMDRYF QIARCFRDED LRADRQPEFT QIDAEMSFCD ENDVMGVVED
260 270 280 290 300
LLQEIFKAVG HTISKPFKRM PYKEAMENYG SDKPDLRFEL PLIEVGDCFR
310 320 330 340 350
DSSNAIFSNT AKDPKNKRIK ALNVKGADAL FSRSVLKELE EFVRQFGAKG
360 370 380 390 400
LAYLQIKEDE IKGPLVKFLS EKGLKNILER TDAQVGDIVF FGAGDKKIVL
410 420 430 440 450
DYMGRLRLKV AETLDLIDKD ALNFLWVVNF PMFEKTENGY HAAHHPFTMP
460 470 480 490 500
KNIECEDIEE VEAHAYDVVL NGVELGGGSI RIHKEEMQKK VFEKINIHEE
510 520 530 540 550
EAQKKFGFLL EALKFGAPPH GGFAIGFDRL IMLMTKSHSI RDVIAFPKTQ
560 570
KASCLLTNAP SPINEEQLRE LHIRLRK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE000511 Genomic DNA Translation: AAD07682.1 |
PIRi | A64597 |
RefSeqi | NP_207412.1, NC_000915.1 WP_001256425.1, NC_018939.1 |
Genome annotation databases
EnsemblBacteriai | AAD07682; AAD07682; HP_0617 |
KEGGi | heo:C694_03190 hpy:HP0617 |
PATRICi | fig|85962.47.peg.665 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE000511 Genomic DNA Translation: AAD07682.1 |
PIRi | A64597 |
RefSeqi | NP_207412.1, NC_000915.1 WP_001256425.1, NC_018939.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5GRO | X-ray | 2.00 | A/B | 1-104 | [»] | |
SMRi | P56459 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
DIPi | DIP-3174N |
IntActi | P56459, 2 interactors |
MINTi | P56459 |
STRINGi | 85962.C694_03190 |
Proteomic databases
PaxDbi | P56459 |
Genome annotation databases
EnsemblBacteriai | AAD07682; AAD07682; HP_0617 |
KEGGi | heo:C694_03190 hpy:HP0617 |
PATRICi | fig|85962.47.peg.665 |
Phylogenomic databases
eggNOGi | COG0173, Bacteria |
OMAi | YQLDVEM |
Enzyme and pathway databases
BioCyci | HPY:HP0617-MONOMER |
Family and domain databases
Gene3Di | 3.30.1360.30, 1 hit |
HAMAPi | MF_00044, Asp_tRNA_synth_type1, 1 hit |
InterProi | View protein in InterPro IPR004364, Aa-tRNA-synt_II IPR006195, aa-tRNA-synth_II IPR004524, Asp-tRNA-ligase_1 IPR002312, Asp/Asn-tRNA-synth_IIb IPR004115, GAD-like_sf IPR029351, GAD_dom IPR012340, NA-bd_OB-fold IPR004365, NA-bd_OB_tRNA |
PANTHERi | PTHR22594:SF5, PTHR22594:SF5, 1 hit |
Pfami | View protein in Pfam PF02938, GAD, 1 hit PF00152, tRNA-synt_2, 1 hit PF01336, tRNA_anti-codon, 1 hit |
PRINTSi | PR01042, TRNASYNTHASP |
SUPFAMi | SSF50249, SSF50249, 1 hit SSF55261, SSF55261, 1 hit |
TIGRFAMsi | TIGR00459, aspS_bact, 1 hit |
PROSITEi | View protein in PROSITE PS50862, AA_TRNA_LIGASE_II, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SYDND_HELPY | |
Accessioni | P56459Primary (citable) accession number: P56459 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | July 15, 1998 | |
Last modified: | December 2, 2020 | |
This is version 133 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families