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UniProtKB - P56452 (SYA_HELPY)
Protein
Alanine--tRNA ligase
Gene
alaS
Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Functioni
Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain.
UniRule annotationCatalytic activityi
- EC:6.1.1.7UniRule annotation
Cofactori
Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 554 | ZincUniRule annotation | 1 | |
Metal bindingi | 558 | ZincUniRule annotation | 1 | |
Metal bindingi | 656 | ZincUniRule annotation | 1 | |
Metal bindingi | 660 | ZincUniRule annotation | 1 |
GO - Molecular functioni
- alanine-tRNA ligase activity Source: GO_Central
- amino acid binding Source: GO_Central
- aminoacyl-tRNA editing activity Source: GO_Central
- ATP binding Source: GO_Central
- tRNA binding Source: UniProtKB-KW
- zinc ion binding Source: UniProtKB-UniRule
GO - Biological processi
- alanyl-tRNA aminoacylation Source: GO_Central
- negative regulation of transcription, DNA-templated Source: GO_Central
- tRNA modification Source: GO_Central
Keywordsi
Molecular function | Aminoacyl-tRNA synthetase, Ligase, RNA-binding, tRNA-binding |
Biological process | Protein biosynthesis |
Ligand | ATP-binding, Metal-binding, Nucleotide-binding, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: Alanine--tRNA ligaseUniRule annotation (EC:6.1.1.7UniRule annotation)Alternative name(s): Alanyl-tRNA synthetaseUniRule annotation Short name: AlaRSUniRule annotation |
Gene namesi | Name:alaSUniRule annotation Ordered Locus Names:HP_1241 |
Organismi | Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) |
Taxonomic identifieri | 85962 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotation
Cytosol
- cytosol Source: GO_Central
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000075125 | 1 – 847 | Alanine--tRNA ligaseAdd BLAST | 847 |
Proteomic databases
PaxDbi | P56452 |
Interactioni
Protein-protein interaction databases
DIPi | DIP-3216N |
IntActi | P56452, 4 interactors |
MINTi | P56452 |
STRINGi | 85962.C694_06400 |
Family & Domainsi
Domaini
Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs.UniRule annotation
Sequence similaritiesi
Belongs to the class-II aminoacyl-tRNA synthetase family.UniRule annotation
Phylogenomic databases
eggNOGi | COG0013, Bacteria |
OMAi | YHHTMFE |
PhylomeDBi | P56452 |
Family and domain databases
Gene3Di | 3.30.930.10, 1 hit |
HAMAPi | MF_00036_B, Ala_tRNA_synth_B, 1 hit |
InterProi | View protein in InterPro IPR045864, aa-tRNA-synth_II/BPL/LPL IPR002318, Ala-tRNA-lgiase_IIc IPR018162, Ala-tRNA-ligase_IIc_anticod-bd IPR018165, Ala-tRNA-synth_IIc_core IPR018164, Ala-tRNA-synth_IIc_N IPR023033, Ala_tRNA_ligase_euk/bac IPR003156, DHHA1_dom IPR018163, Thr/Ala-tRNA-synth_IIc_edit IPR009000, Transl_B-barrel_sf IPR012947, tRNA_SAD |
Pfami | View protein in Pfam PF02272, DHHA1, 1 hit PF01411, tRNA-synt_2c, 1 hit PF07973, tRNA_SAD, 1 hit |
PRINTSi | PR00980, TRNASYNTHALA |
SMARTi | View protein in SMART SM00863, tRNA_SAD, 1 hit |
SUPFAMi | SSF101353, SSF101353, 1 hit SSF50447, SSF50447, 1 hit SSF55186, SSF55186, 1 hit SSF55681, SSF55681, 1 hit |
TIGRFAMsi | TIGR00344, alaS, 1 hit |
PROSITEi | View protein in PROSITE PS50860, AA_TRNA_LIGASE_II_ALA, 1 hit |
i Sequence
Sequence statusi: Complete.
P56452-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDIRNEFLQF FQNKGHAVYP SMPLVPNDAT LLFTNAGMVQ FKDIFTGIVP
60 70 80 90 100
RPSIPRAASS QLCMRAGGKH NDLENVGYTA RHHTLFEMLG NFSFGDYFKE
110 120 130 140 150
EAILFAWEFV TKNLGFKPKD LYISVHEKDD EAVKLWEKFV PVDRIKKMGD
160 170 180 190 200
KDNFWQMGDS GPCGPCSEIY IDQGEKHFKG SEDYFGGEGD RFLEIWNLVF
210 220 230 240 250
MQYERSNDGV LSPLPKPSID TGMGLERVQA LLEHKLNNFD SSLFAPLMEE
260 270 280 290 300
ISELTSLDYA SEFQPSFRVV ADHARAVAFL LAQGVHFNKE GRGYVLRRIL
310 320 330 340 350
RRALRHGYLM GLKEAFLYKV VGVVCEQFAN THAYLKESKE MVVKECFEEE
360 370 380 390 400
EHFLETLESG MELFNLSLKH LNENKIFDGK IAFKLYDTFG FPLDLTNDML
410 420 430 440 450
RSHGACADMQ GFELCMQEQV KRSKASWKGK QNNADFSAIL NAYAPNVFVG
460 470 480 490 500
YETTECSAKV LGFFDSDFKE ITDANPNQEV WVLLEKTPFY AEGGGAIGDR
510 520 530 540 550
GALFKDNGEV AIVLDTKNFF GLNFSLLEIK KALKKGDQVI AQVSDERFEI
560 570 580 590 600
AKHHSATHLL QSALREVLGS HVSQAGSLVE SKRLRFDFSH AKALNDEELE
610 620 630 640 650
KVEDLVNAQI FKHLNSQVEH MPLNQAKDKG ALALFSEKYA ENVRVVSFKE
660 670 680 690 700
ASIELCGGIH VENTGLIGGF RIVKESGVSS GVRRIEAVCG KAFYQLAKEE
710 720 730 740 750
NKELKNAKTL LKNNDVIAGI NKLKESVKNS QKAPVSMDLP VEKIHGVNLV
760 770 780 790 800
VGVVEQGDIK EMIDRLKSKH ERLLAMVFKK ENERITLACG VKNAPIKANV
810 820 830 840
WANEVAQILG GKGGGRGDFA SAGGKDIENL QAALNLAKNT ALKALEG
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE000511 Genomic DNA Translation: AAD08285.1 |
PIRi | A64675 |
RefSeqi | NP_208033.1, NC_000915.1 WP_000354754.1, NC_018939.1 |
Genome annotation databases
EnsemblBacteriai | AAD08285; AAD08285; HP_1241 |
KEGGi | hpy:HP_1241 |
PATRICi | fig|85962.47.peg.1330 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE000511 Genomic DNA Translation: AAD08285.1 |
PIRi | A64675 |
RefSeqi | NP_208033.1, NC_000915.1 WP_000354754.1, NC_018939.1 |
3D structure databases
SMRi | P56452 |
ModBasei | Search... |
Protein-protein interaction databases
DIPi | DIP-3216N |
IntActi | P56452, 4 interactors |
MINTi | P56452 |
STRINGi | 85962.C694_06400 |
Proteomic databases
PaxDbi | P56452 |
Genome annotation databases
EnsemblBacteriai | AAD08285; AAD08285; HP_1241 |
KEGGi | hpy:HP_1241 |
PATRICi | fig|85962.47.peg.1330 |
Phylogenomic databases
eggNOGi | COG0013, Bacteria |
OMAi | YHHTMFE |
PhylomeDBi | P56452 |
Family and domain databases
Gene3Di | 3.30.930.10, 1 hit |
HAMAPi | MF_00036_B, Ala_tRNA_synth_B, 1 hit |
InterProi | View protein in InterPro IPR045864, aa-tRNA-synth_II/BPL/LPL IPR002318, Ala-tRNA-lgiase_IIc IPR018162, Ala-tRNA-ligase_IIc_anticod-bd IPR018165, Ala-tRNA-synth_IIc_core IPR018164, Ala-tRNA-synth_IIc_N IPR023033, Ala_tRNA_ligase_euk/bac IPR003156, DHHA1_dom IPR018163, Thr/Ala-tRNA-synth_IIc_edit IPR009000, Transl_B-barrel_sf IPR012947, tRNA_SAD |
Pfami | View protein in Pfam PF02272, DHHA1, 1 hit PF01411, tRNA-synt_2c, 1 hit PF07973, tRNA_SAD, 1 hit |
PRINTSi | PR00980, TRNASYNTHALA |
SMARTi | View protein in SMART SM00863, tRNA_SAD, 1 hit |
SUPFAMi | SSF101353, SSF101353, 1 hit SSF50447, SSF50447, 1 hit SSF55186, SSF55186, 1 hit SSF55681, SSF55681, 1 hit |
TIGRFAMsi | TIGR00344, alaS, 1 hit |
PROSITEi | View protein in PROSITE PS50860, AA_TRNA_LIGASE_II_ALA, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | SYA_HELPY | |
Accessioni | P56452Primary (citable) accession number: P56452 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | July 15, 1998 | |
Last modified: | February 23, 2022 | |
This is version 116 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Aminoacyl-tRNA synthetases
List of aminoacyl-tRNA synthetase entries - SIMILARITY comments
Index of protein domains and families