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UniProtKB - P56414 (MOAA_HELPY)
Protein
GTP 3',8-cyclase
Gene
moaA
Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Functioni
Catalyzes the cyclization of GTP to (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate.
UniRule annotationCatalytic activityi
- AH2 + GTP + S-adenosyl-L-methionine = (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine + A + H+ + L-methionineUniRule annotationEC:4.1.99.22UniRule annotation
Cofactori
[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters. Binds 1 [4Fe-4S] cluster coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine and 1 [4Fe-4S] cluster coordinated with 3 cysteines and the GTP-derived substrate.UniRule annotation
: molybdopterin biosynthesis Pathwayi
This protein is involved in the pathway molybdopterin biosynthesis, which is part of Cofactor biosynthesis.UniRule annotationView all proteins of this organism that are known to be involved in the pathway molybdopterin biosynthesis and in Cofactor biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 14 | GTPUniRule annotation | 1 | |
Metal bindingi | 21 | Iron-sulfur (4Fe-4S) 1; 4Fe-4S-S-AdoMetUniRule annotation | 1 | |
Metal bindingi | 25 | Iron-sulfur (4Fe-4S) 1; 4Fe-4S-S-AdoMetUniRule annotation | 1 | |
Binding sitei | 27 | S-adenosyl-L-methionineUniRule annotation | 1 | |
Metal bindingi | 28 | Iron-sulfur (4Fe-4S) 1; 4Fe-4S-S-AdoMetUniRule annotation | 1 | |
Binding sitei | 64 | GTPUniRule annotation | 1 | |
Binding sitei | 68 | S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation | 1 | |
Binding sitei | 95 | GTPUniRule annotation | 1 | |
Binding sitei | 119 | S-adenosyl-L-methionineUniRule annotation | 1 | |
Binding sitei | 155 | GTPUniRule annotation | 1 | |
Binding sitei | 189 | S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenUniRule annotation | 1 | |
Metal bindingi | 249 | Iron-sulfur (4Fe-4S) 2; 4Fe-4S-substrateUniRule annotation | 1 | |
Metal bindingi | 252 | Iron-sulfur (4Fe-4S) 2; 4Fe-4S-substrateUniRule annotation | 1 | |
Metal bindingi | 266 | Iron-sulfur (4Fe-4S) 2; 4Fe-4S-substrateUniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 254 – 256 | GTPUniRule annotation | 3 |
GO - Molecular functioni
- 4 iron, 4 sulfur cluster binding Source: UniProtKB-UniRule
- GTP 3',8'-cyclase activity Source: UniProtKB-UniRule
- GTP binding Source: UniProtKB-UniRule
- metal ion binding Source: UniProtKB-KW
- S-adenosyl-L-methionine binding Source: UniProtKB-UniRule
GO - Biological processi
- Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-UniRule
Keywordsi
Molecular function | Lyase |
Biological process | Molybdenum cofactor biosynthesis |
Ligand | 4Fe-4S, GTP-binding, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding, S-adenosyl-L-methionine |
Enzyme and pathway databases
UniPathwayi | UPA00344 |
Names & Taxonomyi
Protein namesi | Recommended name: GTP 3',8-cyclaseUniRule annotation (EC:4.1.99.22UniRule annotation)Alternative name(s): Molybdenum cofactor biosynthesis protein AUniRule annotation |
Gene namesi | Name:moaAUniRule annotation Ordered Locus Names:HP_0768 |
Organismi | Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) |
Taxonomic identifieri | 85962 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000152967 | 1 – 321 | GTP 3',8-cyclaseAdd BLAST | 321 |
Proteomic databases
PaxDbi | P56414 |
Interactioni
Subunit structurei
Monomer and homodimer.
UniRule annotationProtein-protein interaction databases
STRINGi | 85962.C694_03945 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 5 – 233 | Radical SAM corePROSITE-ProRule annotationAdd BLAST | 229 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG2896, Bacteria |
OMAi | IEFMPIG |
PhylomeDBi | P56414 |
Family and domain databases
Gene3Di | 3.20.20.70, 1 hit |
HAMAPi | MF_01225_B, MoaA_B, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR006638, Elp3/MiaB/NifB IPR013483, MoaA IPR000385, MoaA_NifB_PqqE_Fe-S-bd_CS IPR010505, Mob_synth_C IPR007197, rSAM |
Pfami | View protein in Pfam PF06463, Mob_synth_C, 1 hit PF04055, Radical_SAM, 1 hit |
SMARTi | View protein in SMART SM00729, Elp3, 1 hit |
TIGRFAMsi | TIGR02666, moaA, 1 hit |
PROSITEi | View protein in PROSITE PS01305, MOAA_NIFB_PQQE, 1 hit PS51918, RADICAL_SAM, 1 hit |
i Sequence
Sequence statusi: Complete.
P56414-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLVDSFNRVI DYIRVSVTKQ CNFRCQYCMP ATPLNFFDNE ELLPLDNVLE
60 70 80 90 100
FLKIAIDEGV KKIRITGGEP LLRKGLDEFI AKLHAYNKEV ELVLSTNGFL
110 120 130 140 150
LKKMAKDLKN AGLAQVNVSL DSLKSDRVLK ISQKDALKNT LEGIEESLKV
160 170 180 190 200
GLKLKLNTVV IKSVNDDEIL ELLEYAKNRH IQIRYIEFME NTHAKSLVKG
210 220 230 240 250
LKEREILDLI AQKYQIIEAE KPKQGSSKIY TLENGYQFGI IAPHSDDFCQ
260 270 280 290 300
SCNRIRLASD GKICPCLYYQ DAIDAKEAII NKDTKNIKRL LKQSVINKPE
310 320
KNMWNDKNSE TPTRAFYYTG G
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE000511 Genomic DNA Translation: AAD07817.1 |
PIRi | H64615 |
RefSeqi | NP_207561.1, NC_000915.1 WP_001863441.1, NC_018939.1 |
Genome annotation databases
EnsemblBacteriai | AAD07817; AAD07817; HP_0768 |
KEGGi | hpy:HP_0768 |
PATRICi | fig|85962.47.peg.820 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE000511 Genomic DNA Translation: AAD07817.1 |
PIRi | H64615 |
RefSeqi | NP_207561.1, NC_000915.1 WP_001863441.1, NC_018939.1 |
3D structure databases
SMRi | P56414 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 85962.C694_03945 |
Proteomic databases
PaxDbi | P56414 |
Genome annotation databases
EnsemblBacteriai | AAD07817; AAD07817; HP_0768 |
KEGGi | hpy:HP_0768 |
PATRICi | fig|85962.47.peg.820 |
Phylogenomic databases
eggNOGi | COG2896, Bacteria |
OMAi | IEFMPIG |
PhylomeDBi | P56414 |
Enzyme and pathway databases
UniPathwayi | UPA00344 |
Family and domain databases
Gene3Di | 3.20.20.70, 1 hit |
HAMAPi | MF_01225_B, MoaA_B, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR006638, Elp3/MiaB/NifB IPR013483, MoaA IPR000385, MoaA_NifB_PqqE_Fe-S-bd_CS IPR010505, Mob_synth_C IPR007197, rSAM |
Pfami | View protein in Pfam PF06463, Mob_synth_C, 1 hit PF04055, Radical_SAM, 1 hit |
SMARTi | View protein in SMART SM00729, Elp3, 1 hit |
TIGRFAMsi | TIGR02666, moaA, 1 hit |
PROSITEi | View protein in PROSITE PS01305, MOAA_NIFB_PQQE, 1 hit PS51918, RADICAL_SAM, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MOAA_HELPY | |
Accessioni | P56414Primary (citable) accession number: P56414 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | July 15, 1998 | |
Last modified: | February 23, 2022 | |
This is version 123 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families