UniProtKB - P56395 (CYB5_MOUSE)
Protein
Cytochrome b5
Gene
Cyb5a
Organism
Mus musculus (Mouse)
Status
Functioni
Cytochrome b5 is a membrane-bound hemoprotein functioning as an electron carrier for several membrane-bound oxygenases. It is also involved in several steps of the sterol biosynthesis pathway, particularly in the C-5 double bond introduction during the C-5 desaturation.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 44 | Iron (heme axial ligand)PROSITE-ProRule annotation | 1 | |
Metal bindingi | 68 | Iron (heme axial ligand)PROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- electron transfer activity Source: GO_Central
- enzyme binding Source: MGI
- heme binding Source: GO_Central
- metal ion binding Source: UniProtKB-KW
- stearoyl-CoA 9-desaturase activity Source: MGI
GO - Biological processi
- fatty acid metabolic process Source: MGI
- response to cadmium ion Source: Ensembl
Keywordsi
Biological process | Electron transport, Transport |
Ligand | Heme, Iron, Metal-binding |
Enzyme and pathway databases
Reactomei | R-MMU-196836, Vitamin C (ascorbate) metabolism R-MMU-9609523, Insertion of tail-anchored proteins into the endoplasmic reticulum membrane |
Names & Taxonomyi
Protein namesi | Recommended name: Cytochrome b5 |
Gene namesi | Name:Cyb5a Synonyms:Cyb5 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1926952, Cyb5a |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane By similarity; Single-pass membrane protein By similarity; Cytoplasmic side By similarity
- Microsome membrane By similarity; Single-pass membrane protein By similarity; Cytoplasmic side By similarity
Cytosol
- cytosol Source: MGI
Endoplasmic reticulum
- endoplasmic reticulum Source: MGI
- endoplasmic reticulum membrane Source: MGI
Mitochondrion
- mitochondrion Source: MGI
Other locations
- integral component of membrane Source: UniProtKB-KW
- intracellular membrane-bounded organelle Source: MGI
- membrane Source: MGI
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 109 – 131 | HelicalSequence analysisAdd BLAST | 23 |
Keywords - Cellular componenti
Endoplasmic reticulum, Membrane, MicrosomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000166011 | 2 – 134 | Cytochrome b5Add BLAST | 133 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanine1 Publication | 1 | |
Modified residuei | 7 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 10 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 19 | N6-acetyllysineCombined sources | 1 |
Keywords - PTMi
AcetylationProteomic databases
CPTACi | non-CPTAC-3700 |
EPDi | P56395 |
jPOSTi | P56395 |
PaxDbi | P56395 |
PeptideAtlasi | P56395 |
PRIDEi | P56395 |
TopDownProteomicsi | P56395 |
2D gel databases
REPRODUCTION-2DPAGEi | IPI00230113 P56395 |
SWISS-2DPAGEi | P56395 |
PTM databases
iPTMneti | P56395 |
PhosphoSitePlusi | P56395 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000024646, Expressed in adipose tissue and 303 other tissues |
ExpressionAtlasi | P56395, baseline and differential |
Genevisiblei | P56395, MM |
Interactioni
GO - Molecular functioni
- enzyme binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 224948, 4 interactors |
IntActi | P56395, 5 interactors |
MINTi | P56395 |
STRINGi | 10090.ENSMUSP00000124480 |
Miscellaneous databases
RNActi | P56395, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 9 – 85 | Cytochrome b5 heme-bindingPROSITE-ProRule annotationAdd BLAST | 77 |
Sequence similaritiesi
Belongs to the cytochrome b5 family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0537, Eukaryota |
GeneTreei | ENSGT00940000156770 |
HOGENOMi | CLU_102602_3_3_1 |
InParanoidi | P56395 |
OrthoDBi | 1566561at2759 |
PhylomeDBi | P56395 |
TreeFami | TF314537 |
Family and domain databases
Gene3Di | 3.10.120.10, 1 hit |
InterProi | View protein in InterPro IPR001199, Cyt_B5-like_heme/steroid-bd IPR036400, Cyt_B5-like_heme/steroid_sf IPR018506, Cyt_B5_heme-BS |
Pfami | View protein in Pfam PF00173, Cyt-b5, 1 hit |
PRINTSi | PR00363, CYTOCHROMEB5 |
SMARTi | View protein in SMART SM01117, Cyt-b5, 1 hit |
SUPFAMi | SSF55856, SSF55856, 1 hit |
PROSITEi | View protein in PROSITE PS00191, CYTOCHROME_B5_1, 1 hit PS50255, CYTOCHROME_B5_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
P56395-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAGQSDKDVK YYTLEEIQKH KDSKSTWVIL HHKVYDLTKF LEEHPGGEEV
60 70 80 90 100
LREQAGGDAT ENFEDVGHST DARELSKTYI IGELHPDDRS KIAKPSDTLI
110 120 130
TTVESNSSWW TNWVIPAISA LAVALMYRLY MAED
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketG5E850 | G5E850_MOUSE | Cytochrome b5 | Cyb5a | 98 | Annotation score: | ||
E0CY88 | E0CY88_MOUSE | Cytochrome b5 | Cyb5a | 110 | Annotation score: | ||
A0A494B9D8 | A0A494B9D8_MOUSE | Cytochrome b5 | Cyb5a | 54 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC024341 mRNA Translation: AAH24341.1 |
CCDSi | CCDS29385.1 |
RefSeqi | NP_080073.1, NM_025797.4 |
Genome annotation databases
Ensembli | ENSMUST00000160180; ENSMUSP00000124480; ENSMUSG00000024646 |
GeneIDi | 109672 |
KEGGi | mmu:109672 |
UCSCi | uc008fuw.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC024341 mRNA Translation: AAH24341.1 |
CCDSi | CCDS29385.1 |
RefSeqi | NP_080073.1, NM_025797.4 |
3D structure databases
BMRBi | P56395 |
SMRi | P56395 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 224948, 4 interactors |
IntActi | P56395, 5 interactors |
MINTi | P56395 |
STRINGi | 10090.ENSMUSP00000124480 |
PTM databases
iPTMneti | P56395 |
PhosphoSitePlusi | P56395 |
2D gel databases
REPRODUCTION-2DPAGEi | IPI00230113 P56395 |
SWISS-2DPAGEi | P56395 |
Proteomic databases
CPTACi | non-CPTAC-3700 |
EPDi | P56395 |
jPOSTi | P56395 |
PaxDbi | P56395 |
PeptideAtlasi | P56395 |
PRIDEi | P56395 |
TopDownProteomicsi | P56395 |
Protocols and materials databases
Antibodypediai | 23308, 347 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000160180; ENSMUSP00000124480; ENSMUSG00000024646 |
GeneIDi | 109672 |
KEGGi | mmu:109672 |
UCSCi | uc008fuw.1, mouse |
Organism-specific databases
CTDi | 1528 |
MGIi | MGI:1926952, Cyb5a |
Phylogenomic databases
eggNOGi | KOG0537, Eukaryota |
GeneTreei | ENSGT00940000156770 |
HOGENOMi | CLU_102602_3_3_1 |
InParanoidi | P56395 |
OrthoDBi | 1566561at2759 |
PhylomeDBi | P56395 |
TreeFami | TF314537 |
Enzyme and pathway databases
Reactomei | R-MMU-196836, Vitamin C (ascorbate) metabolism R-MMU-9609523, Insertion of tail-anchored proteins into the endoplasmic reticulum membrane |
Miscellaneous databases
BioGRID-ORCSi | 109672, 1 hit in 17 CRISPR screens |
ChiTaRSi | Cyb5a, mouse |
PROi | PR:P56395 |
RNActi | P56395, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000024646, Expressed in adipose tissue and 303 other tissues |
ExpressionAtlasi | P56395, baseline and differential |
Genevisiblei | P56395, MM |
Family and domain databases
Gene3Di | 3.10.120.10, 1 hit |
InterProi | View protein in InterPro IPR001199, Cyt_B5-like_heme/steroid-bd IPR036400, Cyt_B5-like_heme/steroid_sf IPR018506, Cyt_B5_heme-BS |
Pfami | View protein in Pfam PF00173, Cyt-b5, 1 hit |
PRINTSi | PR00363, CYTOCHROMEB5 |
SMARTi | View protein in SMART SM01117, Cyt-b5, 1 hit |
SUPFAMi | SSF55856, SSF55856, 1 hit |
PROSITEi | View protein in PROSITE PS00191, CYTOCHROME_B5_1, 1 hit PS50255, CYTOCHROME_B5_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CYB5_MOUSE | |
Accessioni | P56395Primary (citable) accession number: P56395 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 10, 2021 | |
This is version 162 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families