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UniProtKB - P56373 (P2RX3_HUMAN)
Protein
P2X purinoceptor 3
Gene
P2RX3
Organism
Homo sapiens (Human)
Status
Functioni
Receptor for ATP that acts as a ligand-gated cation channel (PubMed:27626375).
Plays a role in sensory perception. Required for normal perception of pain. Required for normal taste perception (By similarity).
By similarity1 PublicationSites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 172 | ATPCombined sources1 Publication | 1 | |
Binding sitei | 275 | ATPCombined sources1 Publication | 1 | |
Binding sitei | 299 | ATPCombined sources1 Publication | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 63 – 65 | ATPCombined sources1 Publication | 3 | |
Nucleotide bindingi | 279 – 281 | ATPCombined sources1 Publication | 3 |
GO - Molecular functioni
- ATP binding Source: UniProtKB
- extracellularly ATP-gated cation channel activity Source: UniProtKB
- identical protein binding Source: Ensembl
- purinergic nucleotide receptor activity Source: UniProtKB
GO - Biological processi
- behavioral response to formalin induced pain Source: Ensembl
- cellular response to ATP Source: UniProtKB
- inorganic cation transmembrane transport Source: UniProtKB
- neuromuscular synaptic transmission Source: Ensembl
- neuronal action potential Source: Ensembl
- peristalsis Source: Ensembl
- positive regulation of calcium ion transport into cytosol Source: BHF-UCL
- positive regulation of calcium-mediated signaling Source: BHF-UCL
- positive regulation of sensory perception of pain Source: Ensembl
- protein homotrimerization Source: UniProtKB
- regulation of synaptic plasticity Source: Ensembl
- response to carbohydrate Source: Ensembl
- response to cold Source: Ensembl
- response to heat Source: Ensembl
- response to hypoxia Source: Ensembl
- response to mechanical stimulus Source: Ensembl
- sensory perception of taste Source: Ensembl
- signal transduction Source: ProtInc
- urinary bladder smooth muscle contraction Source: Ensembl
Keywordsi
Molecular function | Ion channel, Ligand-gated ion channel, Receptor |
Biological process | Ion transport, Transport |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
PathwayCommonsi | P56373 |
Reactomei | R-HSA-139853, Elevation of cytosolic Ca2+ levels R-HSA-418346, Platelet homeostasis |
Protein family/group databases
TCDBi | 1.A.7.1.6, the atp-gated p2x receptor cation channel (p2x receptor) family |
Names & Taxonomyi
Protein namesi | Recommended name: P2X purinoceptor 3Short name: P2X3 Alternative name(s): ATP receptor Purinergic receptor |
Gene namesi | Name:P2RX3 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:8534, P2RX3 |
MIMi | 600843, gene |
neXtProti | NX_P56373 |
VEuPathDBi | HostDB:ENSG00000109991 |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication
Endoplasmic reticulum
- rough endoplasmic reticulum Source: Ensembl
Golgi apparatus
- Golgi apparatus Source: Ensembl
Nucleus
- integral component of nuclear inner membrane Source: GO_Central
Plasma Membrane
- integral component of plasma membrane Source: UniProtKB
- integral component of presynaptic membrane Source: Ensembl
- plasma membrane Source: Reactome
Other locations
- dendritic spine Source: Ensembl
- hippocampal mossy fiber to CA3 synapse Source: Ensembl
- membrane raft Source: Ensembl
- neuronal cell body Source: Ensembl
- receptor complex Source: MGI
- Schaffer collateral - CA1 synapse Source: Ensembl
- terminal bouton Source: Ensembl
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 20 | Cytoplasmic1 PublicationAdd BLAST | 20 | |
Transmembranei | 21 – 43 | Helical; Name=11 PublicationAdd BLAST | 23 | |
Topological domaini | 44 – 322 | Extracellular1 PublicationAdd BLAST | 279 | |
Transmembranei | 323 – 341 | Helical; Name=21 PublicationAdd BLAST | 19 | |
Topological domaini | 342 – 397 | Cytoplasmic1 PublicationAdd BLAST | 56 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 5024 |
OpenTargetsi | ENSG00000109991 |
PharmGKBi | PA32863 |
Miscellaneous databases
Pharosi | P56373, Tchem |
Chemistry databases
ChEMBLi | CHEMBL2998 |
DrugBanki | DB01069, Promethazine |
DrugCentrali | P56373 |
GuidetoPHARMACOLOGYi | 480 |
Genetic variation databases
BioMutai | P2RX3 |
DMDMi | 259016295 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000161551 | 1 – 397 | P2X purinoceptor 3Add BLAST | 397 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 107 ↔ 153 | Combined sources1 Publication | ||
Disulfide bondi | 116 ↔ 137 | Combined sources1 Publication | ||
Disulfide bondi | 122 ↔ 147 | Combined sources1 Publication | ||
Glycosylationi | 139 | N-linked (GlcNAc...) asparagineCombined sources1 Publication | 1 | |
Glycosylationi | 170 | N-linked (GlcNAc...) asparagineCombined sources1 Publication | 1 | |
Glycosylationi | 194 | N-linked (GlcNAc...) asparagineCombined sources1 Publication | 1 | |
Disulfide bondi | 203 ↔ 213 | Combined sources1 Publication | ||
Disulfide bondi | 247 ↔ 256 | Combined sources1 Publication | ||
Glycosylationi | 290 | N-linked (GlcNAc...) asparagineCombined sources1 Publication | 1 |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
MassIVEi | P56373 |
PaxDbi | P56373 |
PeptideAtlasi | P56373 |
PRIDEi | P56373 |
PTM databases
GlyGeni | P56373, 4 sites |
iPTMneti | P56373 |
PhosphoSitePlusi | P56373 |
Expressioni
Gene expression databases
Bgeei | ENSG00000109991, Expressed in right testis and 75 other tissues |
ExpressionAtlasi | P56373, baseline and differential |
Genevisiblei | P56373, HS |
Organism-specific databases
HPAi | ENSG00000109991, Tissue enhanced (heart muscle, liver, testis) |
Interactioni
Subunit structurei
Homotrimer (PubMed:27626375). Functional P2XRs are organized as homomeric and heteromeric trimers (By similarity).
By similarity1 PublicationGO - Molecular functioni
- identical protein binding Source: Ensembl
Protein-protein interaction databases
BioGRIDi | 111063, 1 interactor |
STRINGi | 9606.ENSP00000263314 |
Chemistry databases
BindingDBi | P56373 |
Miscellaneous databases
RNActi | P56373, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | P56373 |
SMRi | P56373 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 378 – 397 | DisorderedSequence analysisAdd BLAST | 20 |
Sequence similaritiesi
Belongs to the P2X receptor family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502QUDE, Eukaryota |
GeneTreei | ENSGT01020000230351 |
HOGENOMi | CLU_034469_8_0_1 |
InParanoidi | P56373 |
OMAi | CPAEIDD |
OrthoDBi | 1128763at2759 |
PhylomeDBi | P56373 |
TreeFami | TF328633 |
Family and domain databases
Gene3Di | 2.60.490.10, 1 hit |
InterProi | View protein in InterPro IPR003046, P2X3_purnocptor IPR027309, P2X_extracellular_dom_sf IPR001429, P2X_purnocptor |
PANTHERi | PTHR10125:SF8, PTHR10125:SF8, 1 hit |
PIRSFi | PIRSF005713, P2X_purinoceptor, 1 hit |
PRINTSi | PR01310, P2X3RECEPTOR PR01307, P2XRECEPTOR |
TIGRFAMsi | TIGR00863, P2X, 1 hit |
PROSITEi | View protein in PROSITE PS01212, P2X_RECEPTOR, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
P56373-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNCISDFFTY ETTKSVVVKS WTIGIINRVV QLLIISYFVG WVFLHEKAYQ
60 70 80 90 100
VRDTAIESSV VTKVKGSGLY ANRVMDVSDY VTPPQGTSVF VIITKMIVTE
110 120 130 140 150
NQMQGFCPES EEKYRCVSDS QCGPERLPGG GILTGRCVNY SSVLRTCEIQ
160 170 180 190 200
GWCPTEVDTV ETPIMMEAEN FTIFIKNSIR FPLFNFEKGN LLPNLTARDM
210 220 230 240 250
KTCRFHPDKD PFCPILRVGD VVKFAGQDFA KLARTGGVLG IKIGWVCDLD
260 270 280 290 300
KAWDQCIPKY SFTRLDSVSE KSSVSPGYNF RFAKYYKMEN GSEYRTLLKA
310 320 330 340 350
FGIRFDVLVY GNAGKFNIIP TIISSVAAFT SVGVGTVLCD IILLNFLKGA
360 370 380 390
DQYKAKKFEE VNETTLKIAA LTNPVYPSDQ TTAEKQSTDS GAFSIGH
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketH0YDR6 | H0YDR6_HUMAN | P2X purinoceptor 3 | P2RX3 | 50 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 126 | R → P in CAA68947 (PubMed:9016352).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_034674 | 383 | A → V1 PublicationCorresponds to variant dbSNP:rs2276038Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y07683 mRNA Translation: CAA68947.1 AB016608 mRNA Translation: BAA76515.1 AP000781 Genomic DNA No translation available. BC074792 mRNA Translation: AAH74792.1 BC074793 mRNA Translation: AAH74793.1 BC109287 mRNA Translation: AAI09288.1 |
CCDSi | CCDS7953.1 |
RefSeqi | NP_002550.2, NM_002559.3 XP_011543371.1, XM_011545069.2 XP_011543372.1, XM_011545070.2 |
Genome annotation databases
Ensembli | ENST00000263314.3; ENSP00000263314.2; ENSG00000109991.10 |
GeneIDi | 5024 |
KEGGi | hsa:5024 |
MANE-Selecti | ENST00000263314.3; ENSP00000263314.2; NM_002559.5; NP_002550.2 |
UCSCi | uc001nju.4, human |
Similar proteinsi
Cross-referencesi
Web resourcesi
Wikipedia P2X receptor entry |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y07683 mRNA Translation: CAA68947.1 AB016608 mRNA Translation: BAA76515.1 AP000781 Genomic DNA No translation available. BC074792 mRNA Translation: AAH74792.1 BC074793 mRNA Translation: AAH74793.1 BC109287 mRNA Translation: AAI09288.1 |
CCDSi | CCDS7953.1 |
RefSeqi | NP_002550.2, NM_002559.3 XP_011543371.1, XM_011545069.2 XP_011543372.1, XM_011545070.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5SVJ | X-ray | 2.98 | A | 6-364 | [»] | |
5SVK | X-ray | 2.77 | A/B | 6-364 | [»] | |
5SVL | X-ray | 2.90 | A/B | 6-364 | [»] | |
5SVM | X-ray | 3.09 | A/B | 6-364 | [»] | |
5SVP | X-ray | 3.30 | A/B | 6-364 | [»] | |
5SVQ | X-ray | 3.25 | A | 6-364 | [»] | |
5SVR | X-ray | 3.13 | A | 6-364 | [»] | |
5SVS | X-ray | 4.03 | A | 6-364 | [»] | |
5SVT | X-ray | 3.79 | A | 6-364 | [»] | |
5YVE | X-ray | 3.40 | A | 6-364 | [»] | |
6AH4 | X-ray | 3.30 | A/B/C | 17-363 | [»] | |
6AH5 | X-ray | 3.82 | A/B/C | 17-363 | [»] | |
AlphaFoldDBi | P56373 | |||||
SMRi | P56373 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 111063, 1 interactor |
STRINGi | 9606.ENSP00000263314 |
Chemistry databases
BindingDBi | P56373 |
ChEMBLi | CHEMBL2998 |
DrugBanki | DB01069, Promethazine |
DrugCentrali | P56373 |
GuidetoPHARMACOLOGYi | 480 |
Protein family/group databases
TCDBi | 1.A.7.1.6, the atp-gated p2x receptor cation channel (p2x receptor) family |
PTM databases
GlyGeni | P56373, 4 sites |
iPTMneti | P56373 |
PhosphoSitePlusi | P56373 |
Genetic variation databases
BioMutai | P2RX3 |
DMDMi | 259016295 |
Proteomic databases
MassIVEi | P56373 |
PaxDbi | P56373 |
PeptideAtlasi | P56373 |
PRIDEi | P56373 |
Protocols and materials databases
Antibodypediai | 14096, 249 antibodies from 31 providers |
DNASUi | 5024 |
Genome annotation databases
Ensembli | ENST00000263314.3; ENSP00000263314.2; ENSG00000109991.10 |
GeneIDi | 5024 |
KEGGi | hsa:5024 |
MANE-Selecti | ENST00000263314.3; ENSP00000263314.2; NM_002559.5; NP_002550.2 |
UCSCi | uc001nju.4, human |
Organism-specific databases
CTDi | 5024 |
DisGeNETi | 5024 |
GeneCardsi | P2RX3 |
HGNCi | HGNC:8534, P2RX3 |
HPAi | ENSG00000109991, Tissue enhanced (heart muscle, liver, testis) |
MIMi | 600843, gene |
neXtProti | NX_P56373 |
OpenTargetsi | ENSG00000109991 |
PharmGKBi | PA32863 |
VEuPathDBi | HostDB:ENSG00000109991 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502QUDE, Eukaryota |
GeneTreei | ENSGT01020000230351 |
HOGENOMi | CLU_034469_8_0_1 |
InParanoidi | P56373 |
OMAi | CPAEIDD |
OrthoDBi | 1128763at2759 |
PhylomeDBi | P56373 |
TreeFami | TF328633 |
Enzyme and pathway databases
PathwayCommonsi | P56373 |
Reactomei | R-HSA-139853, Elevation of cytosolic Ca2+ levels R-HSA-418346, Platelet homeostasis |
Miscellaneous databases
BioGRID-ORCSi | 5024, 17 hits in 1065 CRISPR screens |
ChiTaRSi | P2RX3, human |
GeneWikii | P2RX3 |
GenomeRNAii | 5024 |
Pharosi | P56373, Tchem |
PROi | PR:P56373 |
RNActi | P56373, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000109991, Expressed in right testis and 75 other tissues |
ExpressionAtlasi | P56373, baseline and differential |
Genevisiblei | P56373, HS |
Family and domain databases
Gene3Di | 2.60.490.10, 1 hit |
InterProi | View protein in InterPro IPR003046, P2X3_purnocptor IPR027309, P2X_extracellular_dom_sf IPR001429, P2X_purnocptor |
PANTHERi | PTHR10125:SF8, PTHR10125:SF8, 1 hit |
PIRSFi | PIRSF005713, P2X_purinoceptor, 1 hit |
PRINTSi | PR01310, P2X3RECEPTOR PR01307, P2XRECEPTOR |
TIGRFAMsi | TIGR00863, P2X, 1 hit |
PROSITEi | View protein in PROSITE PS01212, P2X_RECEPTOR, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | P2RX3_HUMAN | |
Accessioni | P56373Primary (citable) accession number: P56373 Secondary accession number(s): Q6DK37, Q9UQB6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | September 22, 2009 | |
Last modified: | May 25, 2022 | |
This is version 173 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 11
Human chromosome 11: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families