UniProtKB - P56329 (IF2B_SCHPO)
Probable eukaryotic translation initiation factor 2 subunit beta
tif212
Functioni
eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B (By similarity).
By similarityRegions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 273 – 297 | C4-typeSequence analysisAdd BLAST | 25 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- mRNA binding Source: GO_Central
- translation initiation factor activity Source: PomBase
- translation initiation factor binding Source: GO_Central
GO - Biological processi
- formation of cytoplasmic translation initiation complex Source: GO_Central
- formation of translation preinitiation complex Source: GO_Central
Keywordsi
Molecular function | Initiation factor |
Biological process | Protein biosynthesis |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-SPO-156827, L13a-mediated translational silencing of Ceruloplasmin expression R-SPO-72649, Translation initiation complex formation R-SPO-72695, Formation of the ternary complex, and subsequently, the 43S complex R-SPO-72702, Ribosomal scanning and start codon recognition R-SPO-72731, Recycling of eIF2:GDP |
Names & Taxonomyi
Protein namesi | Recommended name: Probable eukaryotic translation initiation factor 2 subunit betaShort name: eIF-2-beta |
Gene namesi | Name:tif212 ORF Names:SPAC32A11.04c, SPAC6B12.17c |
Organismi | Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) |
Taxonomic identifieri | 284812 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › |
Proteomesi |
|
Organism-specific databases
PomBasei | SPAC32A11.04c, tif212 |
VEuPathDBi | FungiDB:SPAC32A11.04c |
Subcellular locationi
Cytosol
- cytosol Source: PomBase
Other locations
- eukaryotic translation initiation factor 2 complex Source: PomBase
- multi-eIF complex Source: PomBase
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000137414 | 1 – 321 | Probable eukaryotic translation initiation factor 2 subunit betaAdd BLAST | 321 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 45 | Phosphoserine1 Publication | 1 | |
Modified residuei | 137 | Phosphoserine1 Publication | 1 | |
Modified residuei | 138 | Phosphoserine1 Publication | 1 | |
Modified residuei | 139 | Phosphothreonine1 Publication | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P56329 |
PaxDbi | P56329 |
PTM databases
iPTMneti | P56329 |
SwissPalmi | P56329 |
Interactioni
Subunit structurei
Heterotrimer composed of an alpha, a beta and a gamma chain.
GO - Molecular functioni
- translation initiation factor binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 279383, 6 interactors |
STRINGi | 4896.SPAC32A11.04c.1 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 117 | DisorderedSequence analysisAdd BLAST | 117 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 15 – 39 | Basic and acidic residuesSequence analysisAdd BLAST | 25 | |
Compositional biasi | 50 – 82 | Basic and acidic residuesSequence analysisAdd BLAST | 33 |
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 273 – 297 | C4-typeSequence analysisAdd BLAST | 25 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG2768, Eukaryota |
HOGENOMi | CLU_026663_0_3_1 |
InParanoidi | P56329 |
OMAi | YMFAEMG |
PhylomeDBi | P56329 |
Family and domain databases
InterProi | View protein in InterPro IPR045196, IF2/IF5 IPR002735, Transl_init_fac_IF2/IF5_dom IPR016189, Transl_init_fac_IF2/IF5_N IPR016190, Transl_init_fac_IF2/IF5_Zn-bd |
PANTHERi | PTHR23001, PTHR23001, 1 hit |
Pfami | View protein in Pfam PF01873, eIF-5_eIF-2B, 1 hit |
SMARTi | View protein in SMART SM00653, eIF2B_5, 1 hit |
SUPFAMi | SSF100966, SSF100966, 1 hit SSF75689, SSF75689, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MTETEAVVDQ IDLNGALPEK KKKPKKSVAF DDEVDAVSKD GPEASSAGAT
60 70 80 90 100
DEDDSERKSS AKDLTTPVTE GEVDELKDMF SSMKKKKKSK KSSASAEEQT
110 120 130 140 150
EDITTESGEL DFSSMKKKKK KKKSADLSAF EKELEASSTG DATSDLSKQT
160 170 180 190 200
FDSENMGEHA WLKSDRDYYY PELLNRFFTL LRTNNPELAG EKRKYTIVPP
210 220 230 240 250
SVHREGKKTI FANISDISKR MHRSLDHVIQ FLFAELGTSG SVDGSSRLII
260 270 280 290 300
KGRFQQKQIE NVLRRYIVEY VTCKTCKSPD TILTKENRIF FMTCEACGSV
310 320
RSVQAIKTGY QAQIGKRKHV S
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CU329670 Genomic DNA Translation: CAB11076.1 |
PIRi | T39024 |
RefSeqi | NP_593772.2, NM_001019202.2 |
Genome annotation databases
EnsemblFungii | SPAC32A11.04c.1; SPAC32A11.04c.1:pep; SPAC32A11.04c |
GeneIDi | 2542942 |
KEGGi | spo:SPAC32A11.04c |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CU329670 Genomic DNA Translation: CAB11076.1 |
PIRi | T39024 |
RefSeqi | NP_593772.2, NM_001019202.2 |
3D structure databases
SMRi | P56329 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 279383, 6 interactors |
STRINGi | 4896.SPAC32A11.04c.1 |
PTM databases
iPTMneti | P56329 |
SwissPalmi | P56329 |
Proteomic databases
MaxQBi | P56329 |
PaxDbi | P56329 |
Genome annotation databases
EnsemblFungii | SPAC32A11.04c.1; SPAC32A11.04c.1:pep; SPAC32A11.04c |
GeneIDi | 2542942 |
KEGGi | spo:SPAC32A11.04c |
Organism-specific databases
PomBasei | SPAC32A11.04c, tif212 |
VEuPathDBi | FungiDB:SPAC32A11.04c |
Phylogenomic databases
eggNOGi | KOG2768, Eukaryota |
HOGENOMi | CLU_026663_0_3_1 |
InParanoidi | P56329 |
OMAi | YMFAEMG |
PhylomeDBi | P56329 |
Enzyme and pathway databases
Reactomei | R-SPO-156827, L13a-mediated translational silencing of Ceruloplasmin expression R-SPO-72649, Translation initiation complex formation R-SPO-72695, Formation of the ternary complex, and subsequently, the 43S complex R-SPO-72702, Ribosomal scanning and start codon recognition R-SPO-72731, Recycling of eIF2:GDP |
Miscellaneous databases
PROi | PR:P56329 |
Family and domain databases
InterProi | View protein in InterPro IPR045196, IF2/IF5 IPR002735, Transl_init_fac_IF2/IF5_dom IPR016189, Transl_init_fac_IF2/IF5_N IPR016190, Transl_init_fac_IF2/IF5_Zn-bd |
PANTHERi | PTHR23001, PTHR23001, 1 hit |
Pfami | View protein in Pfam PF01873, eIF-5_eIF-2B, 1 hit |
SMARTi | View protein in SMART SM00653, eIF2B_5, 1 hit |
SUPFAMi | SSF100966, SSF100966, 1 hit SSF75689, SSF75689, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | IF2B_SCHPO | |
Accessioni | P56329Primary (citable) accession number: P56329 Secondary accession number(s): Q9UT89 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | August 14, 2001 | |
Last modified: | February 23, 2022 | |
This is version 140 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Schizosaccharomyces pombe
Schizosaccharomyces pombe: entries and gene names - Translation initiation factors
List of translation initiation factor entries - SIMILARITY comments
Index of protein domains and families