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Protein

DNA polymerase epsilon subunit 2

Gene

POLE2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Participates in DNA repair and in chromosomal DNA replication.

Miscellaneous

In eukaryotes there are five DNA polymerases: alpha, beta, gamma, delta, and epsilon which are responsible for different reactions of DNA synthesis.

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • DNA-directed DNA polymerase activity Source: ProtInc

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase
Biological processDNA replication

Enzyme and pathway databases

ReactomeiR-HSA-110314 Recognition of DNA damage by PCNA-containing replication complex
R-HSA-174430 Telomere C-strand synthesis initiation
R-HSA-5651801 PCNA-Dependent Long Patch Base Excision Repair
R-HSA-5656169 Termination of translesion DNA synthesis
R-HSA-5685942 HDR through Homologous Recombination (HRR)
R-HSA-5696397 Gap-filling DNA repair synthesis and ligation in GG-NER
R-HSA-5696400 Dual Incision in GG-NER
R-HSA-6782135 Dual incision in TC-NER
R-HSA-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER
R-HSA-68952 DNA replication initiation
R-HSA-68962 Activation of the pre-replicative complex

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase epsilon subunit 2 (EC:2.7.7.7)
Alternative name(s):
DNA polymerase II subunit 2
DNA polymerase epsilon subunit B
Gene namesi
Name:POLE2
Synonyms:DPE2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

EuPathDBiHostDB:ENSG00000100479.12
HGNCiHGNC:9178 POLE2
MIMi602670 gene
neXtProtiNX_P56282

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi5427
OpenTargetsiENSG00000100479
PharmGKBiPA278

Chemistry databases

ChEMBLiCHEMBL2363042
DrugBankiDB00242 Cladribine

Polymorphism and mutation databases

BioMutaiPOLE2
DMDMi3915676

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000715621 – 527DNA polymerase epsilon subunit 2Add BLAST527

Proteomic databases

EPDiP56282
MaxQBiP56282
PaxDbiP56282
PeptideAtlasiP56282
PRIDEiP56282
ProteomicsDBi56911
56912 [P56282-2]
56913 [P56282-3]

PTM databases

iPTMnetiP56282
PhosphoSitePlusiP56282

Expressioni

Gene expression databases

BgeeiENSG00000100479 Expressed in 134 organ(s), highest expression level in kidney
CleanExiHS_POLE2
ExpressionAtlasiP56282 baseline and differential
GenevisibleiP56282 HS

Organism-specific databases

HPAiHPA027555

Interactioni

Subunit structurei

Component of the epsilon DNA polymerase complex consisting of four subunits: POLE, POLE2, POLE3 and POLE4.1 Publication

Binary interactionsi

Protein-protein interaction databases

BioGridi111423, 37 interactors
ComplexPortaliCPX-2108 epsilon DNA polymerase complex
CORUMiP56282
IntActiP56282, 19 interactors
STRINGi9606.ENSP00000216367

Structurei

Secondary structure

1527
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP56282
SMRiP56282
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP56282

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3818 Eukaryota
ENOG410XP7N LUCA
GeneTreeiENSGT00390000012435
HOGENOMiHOG000265254
HOVERGENiHBG061290
InParanoidiP56282
KOiK02325
OMAiPNHFVKT
OrthoDBiEOG091G05Q6
PhylomeDBiP56282
TreeFamiTF103007

Family and domain databases

InterProiView protein in InterPro
IPR007185 DNA_pol_alpha/epsilon_bsu
IPR024639 DNA_pol_e_bsu_N
IPR016266 POLE2
PfamiView protein in Pfam
PF04042 DNA_pol_E_B, 1 hit
PF12213 Dpoe2NT, 1 hit
PIRSFiPIRSF000799 DNA_pol_eps_2, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: P56282-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAPERLRSRA LSAFKLRGLL LRGEAIKYLT EALQSISELE LEDKLEKIIN
60 70 80 90 100
AVEKQPLSSN MIERSVVEAA VQECSQSVDE TIEHVFNIIG AFDIPRFVYN
110 120 130 140 150
SERKKFLPLL MTNHPAPNLF GTPRDKAEMF RERYTILHQR THRHELFTPP
160 170 180 190 200
VIGSHPDESG SKFQLKTIET LLGSTTKIGD AIVLGMITQL KEGKFFLEDP
210 220 230 240 250
TGTVQLDLSK AQFHSGLYTE ACFVLAEGWF EDQVFHVNAF GFPPTEPSST
260 270 280 290 300
TRAYYGNINF FGGPSNTSVK TSAKLKQLEE ENKDAMFVFL SDVWLDQVEV
310 320 330 340 350
LEKLRIMFAG YSPAPPTCFI LCGNFSSAPY GKNQVQALKD SLKTLADIIC
360 370 380 390 400
EYPDIHQSSR FVFVPGPEDP GFGSILPRPP LAESITNEFR QRVPFSVFTT
410 420 430 440 450
NPCRIQYCTQ EITVFREDLV NKMCRNCVRF PSSNLAIPNH FVKTILSQGH
460 470 480 490 500
LTPLPLYVCP VYWAYDYALR VYPVPDLLVI ADKYDPFTTT NTECLCINPG
510 520
SFPRSGFSFK VFYPSNKTVE DSKLQGF
Length:527
Mass (Da):59,537
Last modified:December 15, 1998 - v2
Checksum:iAFA7AF7C2C0BFF15
GO
Isoform 2 (identifier: P56282-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     83-108: Missing.

Note: No experimental confirmation available.
Show »
Length:501
Mass (Da):56,418
Checksum:iECB4BFD1E2FDAE1C
GO
Isoform 3 (identifier: P56282-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     500-527: GSFPRSGFSFKVFYPSNKTVEDSKLQGF → VRM

Show »
Length:502
Mass (Da):56,784
Checksum:i0F3391E93AB14483
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
U3KQ22U3KQ22_HUMAN
DNA polymerase epsilon subunit 2
POLE2
96Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti11L → P in AAC51920 (PubMed:9405441).Curated1
Sequence conflicti359Missing in AAC51920 (PubMed:9405441).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_04437984H → P1 PublicationCorresponds to variant dbSNP:rs34857719Ensembl.1
Natural variantiVAR_044380456L → V. Corresponds to variant dbSNP:rs34574266Ensembl.1
Natural variantiVAR_044381514P → L1 PublicationCorresponds to variant dbSNP:rs45515094Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_04255183 – 108Missing in isoform 2. 1 PublicationAdd BLAST26
Alternative sequenceiVSP_043796500 – 527GSFPR…KLQGF → VRM in isoform 3. 1 PublicationAdd BLAST28

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF025840 mRNA Translation: AAC51920.1
AF036899 mRNA Translation: AAC39610.1
AF387034
, AF387021, AF387022, AF387023, AF387024, AF387025, AF387026, AF387027, AF387028, AF387029, AF387030, AF387031, AF387032, AF387033 Genomic DNA Translation: AAK72254.1
EF506887 Genomic DNA Translation: ABO43040.1
AK293163 mRNA Translation: BAG56707.1
AL139099 Genomic DNA No translation available.
AL591767 Genomic DNA No translation available.
BC112962 mRNA Translation: AAI12963.1
BC126218 mRNA Translation: AAI26219.1
BC126220 mRNA Translation: AAI26221.1
CCDSiCCDS32073.1 [P56282-1]
CCDS55914.1 [P56282-2]
CCDS55915.1 [P56282-3]
RefSeqiNP_001184259.1, NM_001197330.1 [P56282-2]
NP_001184260.1, NM_001197331.1 [P56282-3]
NP_001335313.1, NM_001348384.1
NP_002683.2, NM_002692.3 [P56282-1]
UniGeneiHs.162777

Genome annotation databases

EnsembliENST00000216367; ENSP00000216367; ENSG00000100479 [P56282-1]
ENST00000539565; ENSP00000446313; ENSG00000100479 [P56282-2]
ENST00000554396; ENSP00000451621; ENSG00000100479 [P56282-3]
GeneIDi5427
KEGGihsa:5427
UCSCiuc001wwu.4 human [P56282-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Web resourcesi

NIEHS-SNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF025840 mRNA Translation: AAC51920.1
AF036899 mRNA Translation: AAC39610.1
AF387034
, AF387021, AF387022, AF387023, AF387024, AF387025, AF387026, AF387027, AF387028, AF387029, AF387030, AF387031, AF387032, AF387033 Genomic DNA Translation: AAK72254.1
EF506887 Genomic DNA Translation: ABO43040.1
AK293163 mRNA Translation: BAG56707.1
AL139099 Genomic DNA No translation available.
AL591767 Genomic DNA No translation available.
BC112962 mRNA Translation: AAI12963.1
BC126218 mRNA Translation: AAI26219.1
BC126220 mRNA Translation: AAI26221.1
CCDSiCCDS32073.1 [P56282-1]
CCDS55914.1 [P56282-2]
CCDS55915.1 [P56282-3]
RefSeqiNP_001184259.1, NM_001197330.1 [P56282-2]
NP_001184260.1, NM_001197331.1 [P56282-3]
NP_001335313.1, NM_001348384.1
NP_002683.2, NM_002692.3 [P56282-1]
UniGeneiHs.162777

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2V6ZNMR-M1-73[»]
5VBNX-ray2.35A/E1-527[»]
ProteinModelPortaliP56282
SMRiP56282
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111423, 37 interactors
ComplexPortaliCPX-2108 epsilon DNA polymerase complex
CORUMiP56282
IntActiP56282, 19 interactors
STRINGi9606.ENSP00000216367

Chemistry databases

ChEMBLiCHEMBL2363042
DrugBankiDB00242 Cladribine

PTM databases

iPTMnetiP56282
PhosphoSitePlusiP56282

Polymorphism and mutation databases

BioMutaiPOLE2
DMDMi3915676

Proteomic databases

EPDiP56282
MaxQBiP56282
PaxDbiP56282
PeptideAtlasiP56282
PRIDEiP56282
ProteomicsDBi56911
56912 [P56282-2]
56913 [P56282-3]

Protocols and materials databases

DNASUi5427
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000216367; ENSP00000216367; ENSG00000100479 [P56282-1]
ENST00000539565; ENSP00000446313; ENSG00000100479 [P56282-2]
ENST00000554396; ENSP00000451621; ENSG00000100479 [P56282-3]
GeneIDi5427
KEGGihsa:5427
UCSCiuc001wwu.4 human [P56282-1]

Organism-specific databases

CTDi5427
DisGeNETi5427
EuPathDBiHostDB:ENSG00000100479.12
GeneCardsiPOLE2
HGNCiHGNC:9178 POLE2
HPAiHPA027555
MIMi602670 gene
neXtProtiNX_P56282
OpenTargetsiENSG00000100479
PharmGKBiPA278
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3818 Eukaryota
ENOG410XP7N LUCA
GeneTreeiENSGT00390000012435
HOGENOMiHOG000265254
HOVERGENiHBG061290
InParanoidiP56282
KOiK02325
OMAiPNHFVKT
OrthoDBiEOG091G05Q6
PhylomeDBiP56282
TreeFamiTF103007

Enzyme and pathway databases

ReactomeiR-HSA-110314 Recognition of DNA damage by PCNA-containing replication complex
R-HSA-174430 Telomere C-strand synthesis initiation
R-HSA-5651801 PCNA-Dependent Long Patch Base Excision Repair
R-HSA-5656169 Termination of translesion DNA synthesis
R-HSA-5685942 HDR through Homologous Recombination (HRR)
R-HSA-5696397 Gap-filling DNA repair synthesis and ligation in GG-NER
R-HSA-5696400 Dual Incision in GG-NER
R-HSA-6782135 Dual incision in TC-NER
R-HSA-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER
R-HSA-68952 DNA replication initiation
R-HSA-68962 Activation of the pre-replicative complex

Miscellaneous databases

ChiTaRSiPOLE2 human
EvolutionaryTraceiP56282
GeneWikiiPOLE2
GenomeRNAii5427
PROiPR:P56282
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000100479 Expressed in 134 organ(s), highest expression level in kidney
CleanExiHS_POLE2
ExpressionAtlasiP56282 baseline and differential
GenevisibleiP56282 HS

Family and domain databases

InterProiView protein in InterPro
IPR007185 DNA_pol_alpha/epsilon_bsu
IPR024639 DNA_pol_e_bsu_N
IPR016266 POLE2
PfamiView protein in Pfam
PF04042 DNA_pol_E_B, 1 hit
PF12213 Dpoe2NT, 1 hit
PIRSFiPIRSF000799 DNA_pol_eps_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiDPOE2_HUMAN
AccessioniPrimary (citable) accession number: P56282
Secondary accession number(s): A0AV55
, A4FU92, A4LBB7, A6NH58, B4DDE6, O43560
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: December 15, 1998
Last modified: October 10, 2018
This is version 157 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
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