UniProtKB - P56221 (SCYD_MAGO7)
Protein
Scytalone dehydratase
Gene
SDH1
Organism
Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast fungus) (Pyricularia oryzae)
Status
Functioni
Scytalone dehydratase; part of the gene cluster that mediates the biosynthesis of dihydroxynaphthalene melanin, a bluish-green pigment and a structural component of the conidial wall (PubMed:9571787). Within the pathway, catalyzes the dehydration of scytalone as well as of vermelone (PubMed:9571787, PubMed:9466791, PubMed:9466792, PubMed:9539706, PubMed:10320327, PubMed:10386945, PubMed:10386946, PubMed:10694394, PubMed:10913266, PubMed:10636235, PubMed:11790103, PubMed:12413868, PubMed:15056895). Is also able to dehydrate the alternate substrate 2,3-dihydro-2,5-dihydroxy-4H-benzopyran-4-one (DDBO) to 5-hydroxy-4H-1-benzopyran-4-one (HBO) (PubMed:9466791, PubMed:9466792, PubMed:10320327, PubMed:10386945, PubMed:11790103).13 Publications
Catalytic activityi
- EC:4.2.1.9413 Publications
Activity regulationi
(N-phenoxypropyl)-carboxamides such as carpropamid and derivatives of norephedrine act as inhibitors of scytalone dehydratase activity.6 Publications
Kineticsi
- KM=15 µM for 2,3-dihydro-2,5-dihydroxy-4H-benzopyran-4-one (DDBO)2 Publications
- KM=33 µM for scytalone1 Publication
- KM=31 µM for vermelone2 Publications
: melanin biosynthesis Pathwayi
This protein is involved in the pathway melanin biosynthesis, which is part of Pigment biosynthesis.1 PublicationView all proteins of this organism that are known to be involved in the pathway melanin biosynthesis and in Pigment biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 30 | Substrate1 Publication | 1 | |
Binding sitei | 50 | Substrate1 Publication | 1 | |
Binding sitei | 53 | Substrate; via carbonyl oxygen1 Publication | 1 | |
Active sitei | 85 | 1 Publication | 1 | |
Active sitei | 110 | 1 Publication | 1 | |
Binding sitei | 131 | Substrate3 Publications | 1 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- scytalone dehydratase activity Source: UniProtKB-EC
GO - Biological processi
- melanin biosynthetic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Lyase |
Biological process | Melanin biosynthesis |
Ligand | Calcium, Metal-binding |
Enzyme and pathway databases
BRENDAi | 4.2.1.94, 3152 |
UniPathwayi | UPA00785 |
Names & Taxonomyi
Protein namesi | Recommended name: Scytalone dehydratase1 Publication (EC:4.2.1.946 Publications)Short name: SD1 Publication Short name: SDH1 Publication |
Gene namesi | Name:SDH1 ORF Names:MGG_05059 |
Organismi | Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast fungus) (Pyricularia oryzae) |
Taxonomic identifieri | 242507 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Magnaporthales › Pyriculariaceae › Pyricularia › |
Proteomesi |
|
Organism-specific databases
VEuPathDBi | FungiDB:MGG_05059 |
Subcellular locationi
Endosome
- Endosome By similarity
Endosome
- endosome Source: UniProtKB-SubCell
Keywords - Cellular componenti
EndosomePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 30 | Y → F: Results in a 9-fold decrease of activity with scytalone as the substrate and increases binding of salicylamide inhibitors. 2 Publications | 1 | |
Mutagenesisi | 31 | D → N: Reduces catalysis 5000-fold and substrate affinity about 4-fold with scytalone as the substrate. 1 Publication | 1 | |
Mutagenesisi | 50 | Y → F: Results in a 500-fold decrease of activity with scytalone as the substrate and increases binding of salicylamide inhibitors. 2 Publications | 1 | |
Mutagenesisi | 53 | F → A: Leads to significantly higher relative substrate specificities (DDBO/vermelone). 1 Publication | 1 | |
Mutagenesisi | 53 | F → L: Affects the binding of inhibitors and has significantly higher relative substrate specificities (DDBO/vermelone). 2 Publications | 1 | |
Mutagenesisi | 69 | M → L: Affects the binding of inhibitors. 1 Publication | 1 | |
Mutagenesisi | 75 | V → A: Affects the binding of inhibitors and reduces more than 200-fold inhibition by carpropamid. 2 Publications | 1 | |
Mutagenesisi | 75 | V → M: Reduces strongly inhibition by carpropamid. 1 Publication | 1 | |
Mutagenesisi | 85 | H → N: Greatly decreases catalytic efficiency and decreases binding to salicylamide inhibitors. 2 Publications | 1 | |
Mutagenesisi | 110 | H → N: Causes a 250-fold decrease of activity and a 6-fold increase in Km with scytalone as the substrate, decreases binding of salicylamide inhibitors, and has significantly higher relative substrate specificities (DDBO/vermelone). 3 Publications | 1 | |
Mutagenesisi | 129 | S → A: Results in a 80-fold decrease of activity and a 7-fold increase in Km with scytalone as the substrate. 1 Publication | 1 | |
Mutagenesisi | 129 | S → T: Results in a 120-fold decrease of activity and a 23-fold increase in Km with scytalone as the substrate. 1 Publication | 1 | |
Mutagenesisi | 131 | N → A: Decreases turnover by nearly 90-fold and increases Km 8-fold with scytalone as the substrate, decreases strongly binding of salicylamide inhibitors, and has significantly higher relative substrate specificities (DDBO/vermelone). 3 Publications | 1 | |
Mutagenesisi | 158 | F → L: Affects the binding of inhibitors. 1 Publication | 1 | |
Mutagenesisi | 162 | F → L: Affects the binding of inhibitors. 1 Publication | 1 |
Chemistry databases
ChEMBLi | CHEMBL2578 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000097639 | 1 – 172 | Scytalone dehydrataseAdd BLAST | 172 |
Expressioni
Inductioni
Interactioni
Subunit structurei
Protein-protein interaction databases
STRINGi | 318829.MGG_05059T0 |
Chemistry databases
BindingDBi | P56221 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P56221 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P56221 |
Family & Domainsi
Sequence similaritiesi
Belongs to the scytalone dehydratase family.Curated
Phylogenomic databases
eggNOGi | ENOG502SNND, Eukaryota |
HOGENOMi | CLU_101889_0_0_1 |
InParanoidi | P56221 |
OMAi | RKCIAPT |
OrthoDBi | 1377897at2759 |
Family and domain databases
InterProi | View protein in InterPro IPR032710, NTF2-like_dom_sf IPR004235, Scytalone_dehydratase |
Pfami | View protein in Pfam PF02982, Scytalone_dh, 1 hit |
PIRSFi | PIRSF024851, SCD1, 1 hit |
SUPFAMi | SSF54427, SSF54427, 1 hit |
i Sequence
Sequence statusi: Complete.
P56221-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGSQVQKSDE ITFSDYLGLM TCVYEWADSY DSKDWDRLRK VIAPTLRIDY
60 70 80 90 100
RSFLDKLWEA MPAEEFVGMV SSKQVLGDPT LRTQHFIGGT RWEKVSEDEV
110 120 130 140 150
IGYHQLRVPH QRYKDTTMKE VTMKGHAHSA NLHWYKKIDG VWKFAGLKPD
160 170
IRWGEFDFDR IFEDGRETFG DK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB004741 mRNA Translation: BAA34046.1 CM001233 Genomic DNA Translation: EHA52765.1 |
RefSeqi | XP_003712572.1, XM_003712524.1 |
Genome annotation databases
EnsemblFungii | MGG_05059T0; MGG_05059T0; MGG_05059 |
GeneIDi | 2675492 |
KEGGi | mgr:MGG_05059 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB004741 mRNA Translation: BAA34046.1 CM001233 Genomic DNA Translation: EHA52765.1 |
RefSeqi | XP_003712572.1, XM_003712524.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1IDP | X-ray | 1.45 | A/B/C | 1-172 | [»] | |
1STD | X-ray | 2.90 | A | 1-172 | [»] | |
2STD | X-ray | 2.10 | A | 1-172 | [»] | |
3STD | X-ray | 1.65 | A/B/C | 10-172 | [»] | |
4STD | X-ray | 2.15 | A/B/C | 10-172 | [»] | |
5STD | X-ray | 1.95 | A/B/C | 10-172 | [»] | |
6STD | X-ray | 1.80 | A/B/C | 10-172 | [»] | |
7STD | X-ray | 1.80 | A/B/C | 10-172 | [»] | |
SMRi | P56221 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 318829.MGG_05059T0 |
Chemistry databases
BindingDBi | P56221 |
ChEMBLi | CHEMBL2578 |
Genome annotation databases
EnsemblFungii | MGG_05059T0; MGG_05059T0; MGG_05059 |
GeneIDi | 2675492 |
KEGGi | mgr:MGG_05059 |
Organism-specific databases
VEuPathDBi | FungiDB:MGG_05059 |
Phylogenomic databases
eggNOGi | ENOG502SNND, Eukaryota |
HOGENOMi | CLU_101889_0_0_1 |
InParanoidi | P56221 |
OMAi | RKCIAPT |
OrthoDBi | 1377897at2759 |
Enzyme and pathway databases
UniPathwayi | UPA00785 |
BRENDAi | 4.2.1.94, 3152 |
Miscellaneous databases
EvolutionaryTracei | P56221 |
PROi | PR:P56221 |
Family and domain databases
InterProi | View protein in InterPro IPR032710, NTF2-like_dom_sf IPR004235, Scytalone_dehydratase |
Pfami | View protein in Pfam PF02982, Scytalone_dh, 1 hit |
PIRSFi | PIRSF024851, SCD1, 1 hit |
SUPFAMi | SSF54427, SSF54427, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SCYD_MAGO7 | |
Accessioni | P56221Primary (citable) accession number: P56221 Secondary accession number(s): A4QTI6, G4N445 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | July 15, 1998 | |
Last modified: | April 7, 2021 | |
This is version 125 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families