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Protein

FAD-linked sulfhydryl oxidase ALR

Gene

Gfer

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

FAD-dependent sulfhydryl oxidase that regenerates the redox-active disulfide bonds in CHCHD4/MIA40, a chaperone essential for disulfide bond formation and protein folding in the mitochondrial intermembrane space. The reduced form of CHCHD4/MIA40 forms a transient intermolecular disulfide bridge with GFER/ERV1, resulting in regeneration of the essential disulfide bonds in CHCHD4/MIA40, while GFER/ERV1 becomes re-oxidized by donating electrons to cytochrome c or molecular oxygen (By similarity).By similarity

Catalytic activityi

2 R'C(R)SH + O2 = R'C(R)S-S(R)CR' + H2O2.

Cofactori

FADPROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei104FADBy similarity1
Binding sitei133FADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi92 – 100FADBy similarity9
Nucleotide bindingi164 – 176FADBy similarityAdd BLAST13
Nucleotide bindingi187 – 188FADBy similarity2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGrowth factor, Oxidoreductase
LigandFAD, Flavoprotein

Names & Taxonomyi

Protein namesi
Recommended name:
FAD-linked sulfhydryl oxidase ALR (EC:1.8.3.2)
Alternative name(s):
Augmenter of liver regeneration
Gene namesi
Name:Gfer
Synonyms:Alr
ORF Names:MNCb-0663
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:107757 Gfer

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002085491 – 198FAD-linked sulfhydryl oxidase ALRAdd BLAST198

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi88Interchain (with C-197)PROSITE-ProRule annotation
Disulfide bondi135 ↔ 138Redox-activePROSITE-ProRule annotation
Disulfide bondi164 ↔ 181PROSITE-ProRule annotation
Disulfide bondi197Interchain (with C-88)PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond

Proteomic databases

EPDiP56213
MaxQBiP56213
PaxDbiP56213
PeptideAtlasiP56213
PRIDEiP56213

PTM databases

iPTMnetiP56213
PhosphoSitePlusiP56213
SwissPalmiP56213

Expressioni

Tissue specificityi

Preferentially expressed in the liver and in testis.1 Publication

Gene expression databases

BgeeiENSMUSG00000040888 Expressed in 283 organ(s), highest expression level in primary oocyte
GenevisibleiP56213 MM

Interactioni

Subunit structurei

Homodimer; disulfide-linked. Interacts with CHCHD4/MIA40.By similarity

GO - Molecular functioni

Protein-protein interaction databases

CORUMiP56213
IntActiP56213, 2 interactors
MINTiP56213
STRINGi10090.ENSMUSP00000049186

Structurei

3D structure databases

ProteinModelPortaliP56213
SMRiP56213
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini88 – 188ERV/ALR sulfhydryl oxidasePROSITE-ProRule annotationAdd BLAST101

Phylogenomic databases

eggNOGiKOG3355 Eukaryota
COG5054 LUCA
GeneTreeiENSGT00390000001979
HOGENOMiHOG000195924
HOVERGENiHBG000235
InParanoidiP56213
KOiK17783
OMAiFWEERWR
OrthoDBiEOG091G0TRW
PhylomeDBiP56213
TreeFamiTF105271

Family and domain databases

Gene3Di1.20.120.310, 1 hit
InterProiView protein in InterPro
IPR039799 ALR/ERV
IPR036774 ERV/ALR_sulphydryl_oxid_sf
IPR017905 ERV/ALR_sulphydryl_oxidase
PANTHERiPTHR12645 PTHR12645, 1 hit
PfamiView protein in Pfam
PF04777 Evr1_Alr, 1 hit
SUPFAMiSSF69000 SSF69000, 1 hit
PROSITEiView protein in PROSITE
PS51324 ERV_ALR, 1 hit

Sequencei

Sequence statusi: Complete.

P56213-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAAPSEPAGF PRGSRFSFLP GGARSEMTDD LVTDARGRGA RHRDDTTPAA
60 70 80 90 100
APAPQGLEHG KRPCRACVDF KSWMRTQQKR DIKFREDCPQ DREELGRHTW
110 120 130 140 150
AFLHTLAAYY PDRPTPEQQQ DMAQFIHIFS KFYPCEECAE DIRKRIGRNQ
160 170 180 190
PDTSTRVSFS QWLCRLHNEV NRKLGKPDFD CSRVDERWRD GWKDGSCD
Length:198
Mass (Da):22,877
Last modified:December 16, 2008 - v2
Checksum:i4828C5D5B2F61054
GO

Sequence cautioni

The sequence AAD36987 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti40A → P in AAD10339 (PubMed:8900538).Curated1
Sequence conflicti49A → S in AAD10339 (PubMed:8900538).Curated1
Sequence conflicti49A → S in AAH23941 (PubMed:15489334).Curated1
Sequence conflicti49A → S in AAD36987 (Ref. 6) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U40494 Genomic DNA Translation: AAD10339.1
AB041561 mRNA Translation: BAA95045.1
AK146579 mRNA Translation: BAE27275.1
CH466606 Genomic DNA Translation: EDL22354.1
BC023941 mRNA Translation: AAH23941.1
AF148688 mRNA Translation: AAD36987.1 Different initiation.
CCDSiCCDS28492.1
RefSeqiNP_075527.2, NM_023040.3
UniGeneiMm.28124

Genome annotation databases

EnsembliENSMUST00000046839; ENSMUSP00000049186; ENSMUSG00000040888
GeneIDi11692
KEGGimmu:11692
UCSCiuc008axr.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U40494 Genomic DNA Translation: AAD10339.1
AB041561 mRNA Translation: BAA95045.1
AK146579 mRNA Translation: BAE27275.1
CH466606 Genomic DNA Translation: EDL22354.1
BC023941 mRNA Translation: AAH23941.1
AF148688 mRNA Translation: AAD36987.1 Different initiation.
CCDSiCCDS28492.1
RefSeqiNP_075527.2, NM_023040.3
UniGeneiMm.28124

3D structure databases

ProteinModelPortaliP56213
SMRiP56213
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

CORUMiP56213
IntActiP56213, 2 interactors
MINTiP56213
STRINGi10090.ENSMUSP00000049186

PTM databases

iPTMnetiP56213
PhosphoSitePlusiP56213
SwissPalmiP56213

Proteomic databases

EPDiP56213
MaxQBiP56213
PaxDbiP56213
PeptideAtlasiP56213
PRIDEiP56213

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000046839; ENSMUSP00000049186; ENSMUSG00000040888
GeneIDi11692
KEGGimmu:11692
UCSCiuc008axr.1 mouse

Organism-specific databases

CTDi2671
MGIiMGI:107757 Gfer

Phylogenomic databases

eggNOGiKOG3355 Eukaryota
COG5054 LUCA
GeneTreeiENSGT00390000001979
HOGENOMiHOG000195924
HOVERGENiHBG000235
InParanoidiP56213
KOiK17783
OMAiFWEERWR
OrthoDBiEOG091G0TRW
PhylomeDBiP56213
TreeFamiTF105271

Miscellaneous databases

PROiPR:P56213
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000040888 Expressed in 283 organ(s), highest expression level in primary oocyte
GenevisibleiP56213 MM

Family and domain databases

Gene3Di1.20.120.310, 1 hit
InterProiView protein in InterPro
IPR039799 ALR/ERV
IPR036774 ERV/ALR_sulphydryl_oxid_sf
IPR017905 ERV/ALR_sulphydryl_oxidase
PANTHERiPTHR12645 PTHR12645, 1 hit
PfamiView protein in Pfam
PF04777 Evr1_Alr, 1 hit
SUPFAMiSSF69000 SSF69000, 1 hit
PROSITEiView protein in PROSITE
PS51324 ERV_ALR, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiALR_MOUSE
AccessioniPrimary (citable) accession number: P56213
Secondary accession number(s): Q8CIF8, Q9JJE6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: December 16, 2008
Last modified: November 7, 2018
This is version 123 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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